• Title/Summary/Keyword: ABF1

Search Result 25, Processing Time 0.021 seconds

Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits

  • Gao, H.;Fan, B.;Zhu, M.J.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.23 no.1
    • /
    • pp.7-12
    • /
    • 2010
  • The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

Securing the Ecological Flow of Gam River through Basin Water Budget Analysis and Optimum Dam Operation (유역 물수지 분석과 댐 운영을 통한 감천의 생태유량 확보방안 연구)

  • Young-Jun Kim;Sang-Hyeok Park;Seo-Yeon Park;Joo-Heon Lee
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2023.05a
    • /
    • pp.237-237
    • /
    • 2023
  • 하천에 흐르는 유량을 활용하기 위한 인간의 요구는 끊임없이 증대되고 있으며, 댐 건설과 같은 대형 구조물의 건설은 인간의 수자원 이용에 대한 편의성을 증가시켜주었으나 본래 자연하천이 갖는 생태적 기능을 상실하게 만들고 다양한 생태 및 환경적 문제점을 초래하였다. 특히 유황의 변화는 수생태계의 구조와 기능에 커다란 변화를 야기하였다. 따라서 본 연구에서는 하천의 이·치수적 기능을 유지하면서 인간과 자연이 더불어 살 수 있는 친환경적인 하천 조성을 위해 적정량의 생태유량 산정 방안에 대해 분석하였다. 연구 대상유역은 댐 건설로 인해 유황변화가 발생하며, 하천유지유량이 고시된 낙동강 지류하천인 감천유역을 대상으로 하였다. 감천의 생태계에 필요한 생태유량 확보 방안 마련을 위해서 1) 생태계 필요유량의 산정, 2) 유역 물수지모형 구축, 3) 적정 생태유량의 공급 및 확보방안 시나리오 마련의 순서로 연구를 진행하였다. 첫 번째, 생태유량 산정을 위해 수문학적 방법과 서식처 모의 방법을 활용하였다. 수문학적 방법은 Tennant, Aquatic Base Flow (ABF) 및 Smakthin 방법으로 생태유량을 산정하였고, 서식처 모의 방법으로는 서식처 모의 모델 중 2차원 수리해석이 가능한 River 2D를 활용하여 대상어종의 생태유량을 산정하였다. 두 번째, 유역 물수지 분석을 위해 K-WEAP 모델을 활용하여 감천유역의 물수지 모형을 구축하였으며, 김천부항댐의 방류량과 감천유역 하류에 위치한 구미시(선주교)의 유량과 비교를 통해 물수지 모델 결과를 검증하였다. 마지막으로 여덟 가지 생태유량 공급 시나리오를 감천유역의 물수지 모형에 적용하여 생태유량의 확보 방안을 평가하고자 하였다. 본 연구에서는 하천 생태계의 건강성 유지를 위해서 필요한 생태유량을 다양한 학술적 방법으로 산정하여 연구 대상하천에 적용 가능성을 평가하였으며, 향후 생태유량을 보다 정량적으로 평가할 수 있는 기초 자료로 활용될 수 있을 것으로 기대된다. 또한 산정된 생태유량을 확보하기 위한 물수지모델의 적용방안을 제시함으로써 하천 및 유역의 수자원관리에 방향을 제시하고자 하였다.

  • PDF

Studies on Feed Values of Oak(Quercus serrata Thunb.) and Pine (Pinus densiflora) Browses (졸참나무지엽과 소나무지엽의 사료가치에 관한 연구)

  • 최순호;김원호;김맹중;허삼남;이성운
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.22 no.1
    • /
    • pp.59-68
    • /
    • 2002
  • Chemical composition, mineral compounds, cellular constituents, digestibility for dry matter, total digestible nutrients, and tannin constituents were investigated to evaluate feed value for oak and pine browses to use effectively browses as forest by products. The results of this study were as follows : Crude protein was 6.00∼7.89% for oak browse and 5.15∼6.06% for pine browse sampled through July to October, It gradually decreased in oak browse but not in pine browse as the growth of tree was progressed stages. Crude fat content of oak and pine browse were 1.90∼2.68% and 6.30∼6.33%. Crude fiber content was 33.3∼35.2% for oak browse and 33.7∼34.8% for pine browse and they tended to increase according to the growth stage of trees. Crude ash was 3.13∼2.78% for oak browse and 2.11 ∼1.93% for pine browse, respectively. Ca, Mg, Mn and Cu content were higher in oak browse than those in pine browse, but P, K, Na, Fe, and Zn content were similar each other. Mineral contents were decreased as the growth of tree was progressed(P<.05). Acid detergent fiber content of pine and oak browse were 47.7∼52.0% and 46.1∼48.1%, and they increased as the tree grew. Neutral detergent fiber content of pine and oak browse were 64.1∼67.9% and 65.0∼66.5%. NDF content was increased according to the tree growth in pine browse but it was the same in oak browse(P<.05). in vitro digestibility of dry matter was 51.7∼48.4% for pine browse that decreased according to the tree growth(p<.05) and 53.0∼5l.4% for oak browse. Total digestible nutrients of oak browse was 50.9∼52.4% and that of pine browse was 47.7∼51.1% which decreased as the tree grew. Oak browse showed the same relative feeding value(RFV) as pine browse, and it decreased as the growth of tree was progressed(P<.05). Tannin contents was 2.96% for oak browse, 6.27% for pine browse. Tannin contents decreased when browses were dried.

Analysis of Genetic Polymorphism by Bloodtyping in Jeju Horse (혈액형에 의한 제주말의 유전적 다형성 분석)

  • Cho Gil-Jae
    • Journal of Life Science
    • /
    • v.15 no.6 s.73
    • /
    • pp.972-978
    • /
    • 2005
  • The present study was carried out to investigate the blood markers of Jeju horses. The redcell cypes (blood groups) and blood protein types (biochemical polymorphisms) were tested from 102 Jeju horses by serological and electrophoretc procedure, and their phenotypes and gene frequencies were estimated. The blood group and biochemical polymorphism phenotypes observed with high frequency were $A^{af}\;(27.45\%$), $C^{a}\;(99.02\%$), $K^{-}\;(97.06\%$), $U^{a}\;(62.75\%$), $P^{b}\;(36.27\%$), $Q^{c}\;(47.06\%$), $D^{cgm/dghm}\;(13.73\%$), $D^{adn/cgm}\;(9.80\%$), $D^{ad/cgm}$\;(8.82\%$), $D^{dghm/dghm}(7.84\%$), $D^{cgm/cgm}(7.84\%$), $AL^{B}\;(48.04\%$), $GC^{F}\;(99.02\%$), $AlB^{K}\;(97.06\%$), $ES^{FI}\;(36.27\%$), $TF^{F2}\;(25.49\%$), $HB^{B1}\;(45.10\%$), and $PGD^{F}\;(86.27\%$) in Jeju horses, respectively. Alleles observed with high gene frequency were $A^{af}$ (0.3726), $A^{C}$ (0.2647), $C^{-}$ (0.5050), $K^{-}$ (0.9853), $U^{-}$ (0.6863), $P^{b}$ (0.4657), $Q^{c}$ (0.5294), $D^{cgm}$ (0.3039), $HB^{B1}$(0.6863), $PGD^{F}$ (0.9265), $AL^{B}$ (0.6912), $ALB^{K}$ (0.9852), $GC^{F}$ (0.9950), $ES^{I}$ (0.5000) and $TF^{F2}$ (0.4950) in Jeju horses, and sfecific alleles, $D^{cgm(f)}$ (0.0196), $HB^{A}$ (0.0147), $HB^{A2}$ (0.0196), $ES^{G}$ (0.0441), $ES^{H}$ (0.0098), $TF^{E}$TF'(0.0246), $TF^{H2}$ (0.0049) and $PGD^{D}$ (0.0098) were detected in Jeju horses. These preliminary results present basic information for detecting the genetic markers in Jeju horse. and developing a system for parentage verification and individuals identification in jeju horses.

Effect of Freezing and Thawing Condition on the Physical Characteristics of Blanched Bean Sprouts as Home Meal Replacement (냉.해동 조건에 따른 간편편이식 콩나물의 물리적 품질 변화)

  • Jang, Min-Young;Jung, You-Kyoung;Min, Sang-Gi;Cho, Eun-Kyung;Lee, Mi-Yeon
    • Culinary science and hospitality research
    • /
    • v.20 no.6
    • /
    • pp.235-244
    • /
    • 2014
  • The purpose of this study was to investigate the effect of freezing and thawing rate on the physical properties of soybean sprouts to improve the quality of processed soybean sprouts during distribution and storage. Cooked soybean sprouts were frozen by air-blast freezing (ABF) system at $-45^{\circ}C$ or natural air convection freezing (NCF) system at $-24^{\circ}C$, then thawed using microwave oven by varying output power (0, 400, 800 and 1,000 W) until $75^{\circ}C$. The quality of soybean sprouts was measured by the water content, hardness and springiness. In addition, the internal microstructure of soybean sprouts was observed by optical microscope. For results, water content of soybean sprouts thawed by 1,000 W in a microwave showed the lowest value after natural air convection freezing. Springiness of soybean sprouts thawed by all amounts of output power was decreased in comparison with control. Hardness was increased only in soybean sprouts thawed by 1,000 W after air-blast freezing. However the gaps between springiness and hardness were relatively small with control at 1,000 W thawing, after air-blast freezing. Internal microstructure of the soybean sprouts was more damaged as freezing and thawing time were increased. In conclusion, high freezing and thawing rate might improves the quality of soy bean sprout, and IQF freezing and 1,000 W of microwave thawing appears to be the optimum condition for frozen HMR production. From the results freezing and thawing process parameters might can be use as quality control parameters as various type of sprout products processing.