• Biomedical Science Letters
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• v.15 no.4
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• pp.309-317
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• 2009

Occupational exposure to inorganic dusts such as coal and silica has been identified as a chronic obstructive pulmonary disease (COPD) risk factor. This risk factor causes lung inflammation and protease-antiprotease imbalance. This abnormal inflammatory response of the lung induces parenchymal tissue destruction and leads to progressive airflow limitation that is characteristics of COPD. The aim of this study was to determine the relationship of proteases such as neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 and antiproteases such as alpha-1 antitrypsin (AAT) and tissue inhibitors of metalloproteinase (TIMP)-1 with lung function. The study population contained 223 retired workers exposed to inorganic dusts. We performed lung function test, including percent of forced expiratory volume in one second ($%FEV_1$) predicted and $%FEV_1$/forced vital capacity (FVC). We analyzed serum MMP-9, AAT, TIMP-1 and plasma NE concentrations by sandwich enzyme immunoassay. NE, AAT, and TIMP-1 concentrations in workers, who had $%FEV_1$<80% predicted, were higher than those of workers who had $%FEV_1{\geq}80%$ (P<0.05). Both AAT and TIMP-1 concentrations in workers with airflow limitation were higher than those of workers with normal airflow (P<0.05). $%FEV_1$ predicted showed significant negative correlation with AAT (r=-0.255, P<0.0l), TIMP-1 (r=-0.232, P<0.01), and NE (r=-0.196, P<0.01). $%FEV_1$/FVC predicted showed significant negative correlation with NE (r=-0.172, P<0.05). From the results of stepwise multiple regression analysis about $%FEV_1$ and $%FEV_1$/FVC, significant independents were NE (r=-0.135, P=0.001) and AAT (r=-0.100, P=0.013) in $%FEV_1$, and NE (r=-0.160, P=0.014) in $%FEV_1$/FVC. In the present study, there were significant correlations between airflow limitation and protease concentration and between airflow limitation and antiprotease concentration. Serum protease and antiprotease concentrations, however, may be affected by the biological and inflammatory responses. It is necessary to evaluate specimens more reflected the effects of proteases and antiproteases in the lung such as lung tissue, bronchoalveolar lavage fluid, and exhaled breath condensate (EBC).

• Journal of the Korean Society for information Management
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• v.20 no.3
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• pp.155-175
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• 2003

The purpose of the study was to construct a system based on the semantic web environment's ontology by utilizing the ontology schema derived from the facet-type Art and Architecture Thesaurus(AAT). The aforementioned ontology schema is based on the Web Ontology Language(OWL), which is being widely considered the standard ontology language for the W3C-centered semantic web environment. Also, the concepts were limited to terms within AAT'S Furniture Facet, and the system was tested using the Chair concept, which is a lower-level facet that has a diverse conceptual relationship and broad vocabulary base. The ontology system is capable of searching for concepts, while controlling the search results by always providing a 'Preferred term' for synonymous terms. In addition, the system provides the user with first, a relationship between the terms centered around the inquiry, and second, related terms along with their classification properties. Also, the system is presented as and application example of the ontology system that constructs a information system that intakes an Instance value and reproduces it into a RDF file. During this process, utilization of multiple ontologies was introduced, and the stored Instance value's meta-data elements were used.

• Journal of Biosystems Engineering
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• v.31 no.4 s.117
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• pp.342-348
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• 2006

Aerial application using an unmanned agricultural helicopter became necessary for both labor saving and timely spraying. In the previous paper, a rotor system was developed and lift capability was evaluated. The experimental results were compared with simulated predictions using the CFD-ACE program. From the simulation, the relative velocity on the top surface of the blade airfoil increased, resulting in the pressure drop. The CFD analyses were revealed that a drag resistance on the leading edge of the airfoil, a wake at the trailing edge, and a positive pressure underneath the bottom surface were observed. As the results of the simulation, total lifts of 56.8, 74.4 and $95.0kg_f$ were obtained at the 6, 8 and $10^{\circ}$ of AAT (angle of attack), respectively. The simulation results agreed reasonably up to $10^{\circ}$ of AAT. However, at a greater AAT $(<12^{\circ})$ the simulated total lift continuously increased to $105kg_f$, comparing with a decreasing experimental total lift due to the lack of engine power. At a stiff angle of $18^{\circ}$ AAT, a wake was observed at the trailing edge of the airfoil. A rated operating condition determined from the previous paper was also verified through the simulation.

• Journal of Food Hygiene and Safety
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• v.15 no.1
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• pp.51-54
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• 2000

In the 5'-flanking region of the D-AAT, AspAT and AlaDH gene, I found three or two pairs of sequences(designated as Pl, P2, P3) which show significant similarity to the E.coli consensus sequences of -35 and -10 for promoters. The spacing between -35 and -10 is 16 to 18bp in all the three putative promoters Pl, P2 and P3 which is in good agreement with the preferred spacer length in E.coli and in B.subtilis. Therefore, the putative promoters may also function to increase the efficiency of transcriptional initiation. The most stable, double-helical“Shine-Dalgarno”pairing is formed with a free energy change(ΔG) of -13.0 kcal/mol, -9.6 kcal/mol, -15.8 kcal/mol.

• Journal of Microbiology
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• v.40 no.1
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• pp.33-37
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• 2002

An enzymatic reaction for the production of D-alanine from D-aspartic acid and pyruvate as substrates by a thermostable D-amino acid aminotransferase (D-AAT) was investigated at various conditions In the temperature range of 40-70$\^{C}$ and pH range of 6.0-9.5. The D-AAT was produced with recombinant E. coli BL21, which hosted the chimeric plasmid pTLK2 harboring the D-AAT from the novel thermophilic Bacillus sp. LK-2. The enzyme reaction was shown to follow the Ping Pong Bi Bi mechanism. The K$\_$m/ values for D-aspartic acid and pyruvate were 4.38 mar and 0.72 mM, respectively. It was observed that competitive inhibition by D-alanine, the product of this reaction, was evident with the inhibition constant K$\_$i/ value of 0.1 mM. A unique feature of this reaction scheme is that the decorboxylation of oxaloacetic acid, one of the products, spontaneously produces pyruvate. Therefore, only a catalytic amount of pyruvate is necessary for the enzyme conversion reaction to proceed. A typical time-course kinetic study skewed that D-alanine up to 88 mM could be produced from 100 mM of D-aspartic acid with a molar yield of 1.0.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.235.1-235
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• 1994

• Journal of the korean veterinary medical association
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• v.40 no.3
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• pp.254-258
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• 2004

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1443-1452
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• 2021

This study aimed to investigate the effects of the multimodal group intervention that combined animal-assisted therapy (AAT) and integrated play therapy (IPT) on depression and self-esteem in undergraduate university students. The subjects were 40 students attending animal-related and social welfare departments of universities located in a metropolitan city. The multimodal intervention program was conducted for eight sessions (twice a week, 60 min each) in the experimental group. Data analysis was conducted using the independent sample t-test, ANCOVA, and paired sample t-test for pre- and post-test. Therefore, the multimodal intervention program applying AAT and IPT showed positive effects on depression and self-esteem in university students. Based on these results, this study proposed the operation of multidisciplinary education and practical and policy utilization methods to reduce depression among university students and help improve their self-esteem.

• The Journal of Engineering Geology
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• v.28 no.2
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• pp.247-258
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• 2018

In the demonstration-scale offshore $CO_2$ storage project, the monitoring team studies geophysical and geochemical monitoring of $CO_2$ injections in the Yeongil Bay, in which a $CO_2$ test injection (about 100t) was performed in January, 2017 and further injections in larger scales are planned for 2018 and 2019. In this study, the development status of the Korea-type Hydro-Geophone OBS (Ocean Bottom Sensor) and the geochemical baseline survey (focused on some anions of sediment pore water) are suggested as the preliminary results of the pre-injection test.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.109-117
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• 1984

Allozyme study for open pollinated seeds of forty nine pitch pine families in an $F_1$-hybrid seed orchard demonstrated that allozyme variants in aspartate aminotransferase(AAT), glutamate dehydrogenase(GDH) and leucine aminopeptidase(LAP) systems are encoded by at Least eight loci; five for AAT, one for GDH and two fur LAP. Allozyme variations showed polymorphisms at seven of the eight loci, except GDH. Average number of alleles examined over six loci were 2.33 and 2.67 for maternal and progeny groups, respectively. Average heterozygosity and genetic diversity computed over six loci were, respectively, 0.235 and 5.409 for maternal tree group, 0.238 and 5.569 for progeny group. The proportion of $F_1$-hybrid seeds estimated by allozyme analysis was 0.77%. The estimated proportion of $F_1$ hybrid seeds by allozyme study is in good agreement with the value 0.73% estimated by morphological study for the proportion of pitch ${\times}$ Loblolly $F_1$ hybrid seedlings at a nursery. Indications for Wahlund effect, high levels of self-fertilization and for non-random matings in the $F_1$ hybrid seed orchard call for cautions in estimating allele frequency changes and mating probabilities for the parental and progeny groups.