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The Study on the Dilution Time of Radioactive Tracer in Estradiol Measurement (Estradiol 검사 시 방사성 추적자의 희석시간에 대한 고찰)

  • Lee, Hae Yeon;Seo, Han Kyung;Jang, Yi Sun;Kim, Hee Jeoung
    • The Korean Journal of Nuclear Medicine Technology
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    • v.21 no.2
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    • pp.44-48
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    • 2017
  • Purpose Estradiol (E2) is a steroid hormone mainly produced in women and is a useful indicator for diagnosis of gynecological diseases, menstrual cycle, menopause, and precocious puberty. E2 measurement is performed by diluting the $^{125}I$ radioactive tracer and tracer buffer in the kit. However, It was not precisely specified when the period of tracer is available after activating. The purpose of this study was to determine the appropriate dilution time based on the measurement value with dilution time. Materials and Methods From December 2016 to February 2017, 60 E2 samples with concentrations ranging from 8 to 4577 pg/mL were divided into low, medium, and high concentrations. Dilution of the $^{125}I$ tracer was performed on a 230 RPM agitator for 30 minutes, 1 hour 30 minutes, and 2 hours 30 minutes, respectively. 24 hour dilution was gently shaken and refrigerated. To verify the difference and significance of the results according to the dilution time, a test of normality was performed using SPSS 18.0 and analyzed by Kruskal-Wallis test. The measured value according to the dilution time was compared with the interquartile range of the absolute error. Results The results of Kruskal-Wallis test were not significant (P>0.05). Measurement results are showed as interquartile range of absolute error. At low concentration, it is 0.052 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.105 between 30 minutes and 1 hour 30 minutes. At medium concentration, 0.062 between 30 minutes and 1 hour 30 minutes, and 0.038 between 1 hour 30 minutes and 2 hours 30 minutes. At high concentration, it is 0.029 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.06 between 2 hours 30 minutes and 24 hours. Conclusion There were no statistically significant differences. However, the change in the measured value is the smallest between 1 hour and 30 minutes to 2 hours and 30 minutes. Therefore, we recommend diluting time between 1 hour 30 minutes and 2 hours 30 minutes.

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Acute Ecotoxicity Evaluation of 3 Emulsifiable Concentrates Containing Garlic Extract, Zanthoxylum Extract, and Lemon Grass Oil Originated from Plant (식물추출물 마늘 추출액, 잔톡실럼 정유, 레몬그라스 정유 함유 유제 3종의 생태독성평가)

  • You, Are-Sun;Hong, Soon-Sung;Jeong, Mihye;Park, Kyung-Hun;Chang, Hee-Seop;Lee, Je Bong;Park, Jae-Yup
    • The Korean Journal of Pesticide Science
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    • v.16 no.4
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    • pp.376-382
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    • 2012
  • Environment-friendly agro-materials are are likely to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. This study was also conducted so as to assess ecotoxicity for Emulsifiable concentrate (EC) containing 30% of garlic extract or two plant essential oils (Zanthoxylum, Lemongrass) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The $EC_{50}$ values for of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC to Daphina magna were 3.3, 10, and $10mg\;L^{-1}$, respectively. The category of garlic extract 30% EC was moderately toxic, while those of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were slightly toxic according to standard of USEPA. $EC_{50}$ for both of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than $10mg\;L^{-1}$ then they were considered as slightly toxicity. In case of acute toxicity test to fish, $LC_{50}$ of garlic extract 30% EC was $3.3mg\;L^{-1}$. Zanthoxylum oil 30% EC and lemongrass oil 30% EC indicated $LC_{50}$ > $10mg\;L^{-1}$. Classification of acute toxicity to all test substances was in Korea criteria. Acute contact and oral toxicity test to Honeybee were conducted. As a result, $LD_{50}$ of all test substances were more than 100 a.i. ${\mu}g\;bee^{-1}$ in the acute contact test while $LD_{50}$ of garlic extract 30% EC was 4.4 a.i. ${\mu}g\;bee^{-1}$ and $LD_{50}$ of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 100 a.i. ${\mu}g\;bee^{-1}$. In case of acute toxicity test to earthworm, $LC_{50}$ of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC were 267, 592, and $430mg\;kg^{-1}$, respectively. In conclusion, if the safety for earthworm is confirmed, these substances are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

Influence of 30% Oxygen on Heart Rate and $SPO_2$ during Cycle Exercise in Healthy Subjects (30%의 고농도 산소가 정상 성인의 사이클 운동 시 심박동률과 혈중 산소 포화도에 미치는 영향)

  • You Ji-Hye;Yi Jeong-Han;Sohn Jin-Hun;Chung Soon-Cheol
    • Science of Emotion and Sensibility
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    • v.9 no.1
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    • pp.1-7
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    • 2006
  • The purpose of this study is to analyze the effects of the inhalation of 30% oxygen on heart rate and oxygen saturation ($SPO_2$) during cycle isokinetic exercise, in comparison with the inhalation of 21% oxygen. This study used oxygen supply equipment that can supply each of 21% and 30% oxygen constantly at a rate of 8 liter/min. Ten healthy male college students ($25.2{\pm}2.2$ years) participated in the experiment twice, one for 21% oxygen and the other for 30% oxygen. Each experiment was composed of three sections (a total of 18 minutes), which were composure (2 minutes), cycle isokinetic exercise at a speed of $20{\pm}1km/h$ (10 minutes) and recovery (6 minutes). 21% or 30% oxygen was supplied only during the sections of isokinetic exercise and restoration. Heart rate during isokinetic exercise and recovery was lower with the inhalation of 30% oxygen than with that of 20% oxygen but no difference was observed in $SPO_2$. $SPO_2$ was not different possibly because the same work load was applied to the group of 21% oxygen and that of 30% oxygen. Heart rate was reduced with the inhalation of 30% oxygen possibly because a larger quantity of oxygen was supplied at the same work load.

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Floral Visitors and Nectar Secretion of the Japanese Camellia, Camellia Japonica L.

  • Rho, Jeong-Rae;Choe, Chun-Choe
    • Animal cells and systems
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    • v.7 no.2
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    • pp.123-125
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    • 2003
  • We studied the nectar secretion of the Japanese Camellia (Camellia japonica L.), an evergreen tree and observed its floral visitors during the day. The mean volume of nectar secreted, during daylight (08:31 to 16:30 h) was 30.26$\pm8,29ml$ (SD) (n = 27). During the late afternoon and overnight (16:31 to 08:30 following day), 100.54$\pm54.85$(n = 27) of nectar was secreted. Total volume measured when flowers were sampled once every two hours for an eight-hour period was approximately one-half the volume which was measured when the flowers were sampled only once after eight hours. The mean nectar volume secreted was 8.55$\pm8.3ml$(n = 30) between 08:31 to 10:30, 4.38$\pm$6.1 ml (n = 30) between 10:31 to 12:30, 4.6$\pm5.4ml$(n = 30) between 12;31 to 14:30, and 4.02$\pm3.5ml$(n = 30) between 14:31 to 16:30 hours. During the day, Japanese Camellia flowers were principally visited by the Japanese white-eye (Zosterops erythropleura erythropleura 5.), a native bird, although insects and squirrels also visited to a lesser degree.

Physiological and molecular analysis of OsTPS30 by gamma irradiation

  • Kim, Se Won;Jung, In Jung;Kim, Sang Hoon;Choi, Hong-Il;Kang, Si-Yong;Kim, Jin-Baek
    • Journal of Plant Biotechnology
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    • v.46 no.2
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    • pp.88-96
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    • 2019
  • Terpenes constitute a large class of secondary metabolites in plants. The Oryza sativa terpene synthase is a vital gene in plant defense response. In this study, the molecular and physiological functions of Oryza sativa terpene synthase 30 (OsTPS30, LOC_Os08g07080) were investigated after exposure of the seeds and plants to gamma-rays. The OsTPS30 expression was slightly induced at 200 Gray (Gy), but was significantly induced at 400 Gy. The total terpenoid was synthesized more in OsTPS30-overexpressing (OX-OsTPS30) Arabidopsisthaliana plants than in wild-type (WT) plants. The OX-OsTPS30 plants exhibited resistance to gamma-rays, as compared to WT. The OX-OsTPS30 plants had significantly increased height and weight after gamma irradiation. Additionally, the activity of antioxidant enzymes was increased more in OX OsTPS30 plants than in WT plants after gamma irradiation. Furthermore, the OsTPS30-GFP fusion protein was mostly localized in the chloroplast, suggesting that OsTPS30 is putative MEP pathway-related terpene synthase.

A survey on the leggings wearing condition of down-aging women in their 30s and 40s (다운에이징 30-40대 여성의 레깅스 착용실태조사)

  • Eun Kyong Kim
    • Journal of the Korea Fashion and Costume Design Association
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    • v.24 no.4
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    • pp.41-56
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    • 2022
  • This study aims to investigate the actual condition of wearing leggings and the preferred design of down-aging women in their 30s and 40s who have young sensibility, but are starting to enter a middle-aged body type. By analyzing the problems experienced when wearing leggings in this age group, this study intends to provide basic data for the development of a middle-aged female leggings pattern with an improved fit. Therefore, a survey was conducted on 214 women in their 30s and 40s. The results of the survey are as follows. Among down-aging women who want to look younger, leggings are becoming an essential item, but most leggings brands are producing leggings with patterns suitable for women in their 20s. The results of this study also showed that down-aging women in their 30s and 40s mainly purchase and prefer body-fitting leggings. However, they are experiencing discomfort due to 'Y-zone accentuate', 'undergarment accentuate', 'cloth stucking', and 'waist band curling and tightening'. The results indicate that women in their 30s and 40s, who are starting to change to a middle-aged body type, have fit problems. Women in their 20s generally prefer functions that improve the body line, while those in their 30s and 40s prefer functions that improve movement and cover problems caused by the abdomen and the Y-zone area. Therefore, when designing leggings for down-aging women in their 30s and 40s, based on body-fitting leggings, a material with excellent elasticity must be selected. Also develop patterns that solves 'Y-zone accentuate', 'undergarment accentuate', 'cloth stucking', and 'waist band curling and tightening' problems.

Evaluation of Ammonia Emission from Liquid Pig Manure Composting System with Forced Aeration (돈분뇨의 호기적 액비화 과정에서 암모니아 휘산량 평가)

  • Kim, Tae-Young;Kim, Song-Yeob;Chang, Hong-Hee;Yun, Hong-Bae;Lee, Yong-Bok
    • Korean Journal of Environmental Agriculture
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    • v.32 no.4
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    • pp.366-368
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    • 2013
  • BACKGROUND: Composting is the most frequently used waste management process for animal manure in Korea's livestock industry. In the composting process, a large amount of nitrogen (N) is volatilized to the atmosphere as amonia ($NH_3$). However, quantitative information of $NH_3$ emission from composting of liquid manure is required to obtain emission factors for management of livestock manure in Korea. METHODS AND RESULTS: To evaluate the $NH_3$ emission from composting of liquid manure affected by aeration, we conducted composting of liquid pig manure with three forced aeration systems. The aeration conditions were continuous (A60), cycle of 30 min aeration and 30 min pause (A30S30) and without aeration(A0). All treatments were aerated 12 hour per day with these aeration systems. The total ratio of $NH_3$ volatilization loss to total N content in liquid manure throughout composting period was estimated to 19.9% for A0 treatment, 25.9% for A30S30 treatment and 36.3% for A60 treatment. The A30S30 and A60 aeration systems increased $NH_3$ volatilization by 30.2 and 82.3% compared with systems without forced aeration. CONCLUSION(S): Ammonia emission during liquid pig manure composting was highly affected by forced aeration. The development of liquid pig manure composting systems with forced aeration would be considered both reducing ammonia emission and efficiency of composting.

Effects of Liquid Culture of Agaricus blazei Murill on Lipid Metabolism in Rats Fed Cholesterol Diet. (신령버섯 균사체 액체배양액이 고콜레스테롤혈증 흰쥐의 지질대사에 미치는 영향)

  • 고진복;김재영
    • Journal of Life Science
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    • v.14 no.4
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    • pp.531-536
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    • 2004
  • The effects of liquid culture of Agaricus blazei Murill on the weight gains, serum and hepatic lipid concentrations were studied in male rats. Sprague-Dawley rats (7 weeks old) were given four different types of diets for 6 weeks, respectively: a normal diet group, a high fat control diet group (normal diet+15% lard +0.5% cholesterol), a 30% or 40% A. blazei diet groups (high fat control diet+30% or 40% A. blazei in water) according to the levels of A. blazei supplementation. The body weight gains, food intake, food efficiency ratios, and the liver, kidney, and epididymal fat pad weights of the rats fed 30% or 40% A. blazei diets were similar to those of the rats fed high fat control diet. The concentrations of serum total cholesterol, LDL-cholesterol and atherogenic index in rats fed the 30% or 40% A. blazei diets were significantly decreased compared with those of rats fed the high fat control diet. The HDL-cholesterol/total-cholesterol ratios of the rats fed 30% or 40% A. blazei diet were significantly increased compared with those of rats fed the high fat control diet. The fecal excretion of total lipid . in the rats fed 40% A. blazei diet was significantly increased compared with those of rats fed the high fat control diet. The concentrations of serum total lipid in the rats fed 40% A. blazei diet was significantly lower than that in the rats fed high fat control diet. But the concentrations of serum HDL-cholesterol, hepatic total cholesterol and triglyceride of rats fed the 30 or 40% A. blazei diets were similar to those of rats fed the high fat control diet. These results showed that the 30 or 40% A. blazei diets feeding decreased the total cholesterol, LDL-cholesterol and atherogenic index, and increased the HDL-cholesterol/total-cholesterol ratio in serum of rats.

A Research Study on the Wearing Conditions and Preferences of Slim Fit Tailored Jacket Consumers - Focused on the comparison by body shape among the women in their 30s - (슬림 핏 테일러드 재킷의 소비자 착용실태 및 선호 조사연구 - 30대 여성 체형별 비교 중심으로 -)

  • Jeong, JaeChul;Park, SunKyung
    • Fashion & Textile Research Journal
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    • v.17 no.4
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    • pp.588-596
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    • 2015
  • This study aims to provide the basic reference necessary in improving the fitness of a ready-to-wear brand jacket through a research on the wearing conditions and preferences regarding a tailored jacket by body shape of the women in their 30s. The following results were drawn by implementing the research method of the self-administered type survey targeting 201 women in their 30s from August 14~28, 2014. First, the female consumers in their 30s preferred one button slim fit jacket when purchasing a tailored jacket and chose a close-fitting size. Second, the slim fit tailored jacket consumers preferred a jacket with some extra portion in the bust and hip areas but tightly fit in the waist area. Third, when purchasing a jacket under the ready-to-wear size system, the women with a triangle shape similar to a standard body silhouette had less instances of mending a jacket while the women with a big or small rectangle shape different from a standard body silhouette had more experiences of mending a jacket. Accordingly, it is expected of the manufacturers to increase the fitness satisfaction of the consumers by identifying the problems concerning the fitness and suggesting the improvement plans to produce a jacket of a good fitness reflecting the properties of different body shapes through such research on the wearing conditions and preferences regarding a tailored jacket by body shape of the women in their 30s.

Corrosion Characteristics of Ti alloy for Removable Partial Denture (국소의치용 티타늄 합금의 부식 특성)

  • Kim, Jeong-Jae;Kim, Won-Gi
    • The Journal of the Korea Contents Association
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    • v.14 no.4
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    • pp.237-242
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    • 2014
  • In this study, surface characteristics and corrosion behaviors have been investigated in addition to Zr elements on the low elastic modulus Ti-30Ta alloy. Low elastic modulus Ti-30Ta-xZr(x : 3, 7 and 15 wt %) alloys were prepared by arc melting and then heat treated at $1000^{\circ}C$ for 24 hrs in an argon atmosphere. Microstructures of the alloys were examined by field emission scanning electron microscope(FE-SEM) and X-ray diffractometer(XRD). Electrochemical experiments were performed using a conventional three-electrode configuration with a sample working electrode, a high density carbon counter electrode and a saturated calomel reference electrode. According to the result of polarization behavior in the Ti-30Ta-xZr alloys, the current density of homogenized Ti-30Ta-15Zr in the passive region was lower than the other alloys.