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Development of an Official Analytical Method for Determination of Phorate and its Metabolites in Livestock Using LC-MS/MS (LC-MS/MS를 이용한 축산물 중 Phorate 및 대사산물 5종 동시분석법 개발)

  • Ko, Ah-Young;Kim, Heejung;Jang, Jin;Lee, Eun Hyang;Ju, Yunji;Noh, Mijung;Kim, Seongcheol;Park, Sung-Won;Chang, Moon-Ik;Rhee, Gyu-Seek
    • Journal of Food Hygiene and Safety
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    • v.30 no.3
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    • pp.272-280
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    • 2015
  • A simultaneous official method was developed for the determination of phorate and its metabolites (phorate sulfoxide, phorate sulfone, phorate oxon, phorate oxon sulfoxide, phorate oxon sulfone) in livestock samples. The analytes were quantified and confirmed via liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Phorate and its metabolites were extracted from beef and milk samples with acidified acetonitrile (containing 1% acetic acid) and partitioned with anhydrous magnesium sulfate. Then, the extract was purified through primary secondary amine (PSA) and C18 dispersive sorbent. Matrix matched calibration curves were linear over the calibration ranges (0.005-0.5 mg/L) for all the analytes into blank extract with $r^2$ > 0.996. For validation purposes, recovery studies were carried out at three different concentration levels (beef 0.004, 0.04 and 0.2 mg/kg; milk 0.008, 0.04 and 0.2 mg/kg, n = 5). The recoveries were within 79.2-113.9% with relative standard deviations (RSDs) less than 19.2% for all analytes. All values were consistent with the criteria ranges requested in the Codex guidelines. The limit of quantification was quite lower than the maximum residue limit (MRL) set by the Ministry of Food and Drug Safety (0.05 mg/kg). The proposed analytical method was accurate, effective and sensitive for phorate and its metabolites determination and it will be used to as an official analytical method in Korea.

Relationship of Oral Health Awareness to Oral Health Indexes among Adults (성인의 구강건강인식과 구강보건지수와 관계)

  • Shin, Myong-Suk;Hwang, Mi-Yeong;Kim, Soo-Kyung
    • Journal of dental hygiene science
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    • v.12 no.6
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    • pp.607-616
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    • 2012
  • The purpose of this study was to examine the self-rated oral health status and oral health concern of 6,094 adults over the age of 19, which were both related to subjective oral health awareness, based on the second-year (2008) raw data of the 4th National Health and Nutrition Survey. 1. As for subjective oral health awareness, 49.4 percent replied they were in bad oral health when they were asked about self-rated oral health status. Regarding oral health concern, 62.6 percent answered they were sort of concerned about oral health. 2. As to oral health indexes by sociodemographic characteristics, there were statistically significant differences in oral health indexes according to gender, age, academic credential, monthly mean household income, frequency of eating between meals and toothbrushing frequency. Smoking made no statistically significant differences to oral health indexes (p<0.000). 3. Concerning self-rated oral health status by sociodemographic characteristics, no significant differences were found according to gender, age and academic credential, and there were statistically significant differences according to monthly mean household income and smoking (p<0.000), frequency of eating between meals (p<0.018), toothbrushing frequency (p<0.003). 4. In relation to oral health concern by sociodemographic characteristics, gender and smoking made no significant differences, and statistically significant differences were found according to age (p<0.003), academic credential, monthly mean household income, frequency of eating between meals and toothbrushing frequency (p<0.000). 5. In regard to the relationship between subjective oral health awareness and oral health indexes, none of the oral health indexes had a significant relationship to self-rated oral health status, and there were statistically significant differences in oral health concern according to functioning teeth index (p<0.011) and community periodontal index (p<0.017).

Feeding Habits of the Largehead Hairtail, Trichiurus japonicus in the Yellow Sea of Korea (우리나라 서해에서 출현하는 갈치(Trichiurus japonicus)의 식성)

  • Seong, Gi Chang;Kim, Do-Gyun;Kang, Da Yeon;Jin, Suyeon;Kim, Hoseung;Soh, Ho Young;Baeck, Gun Wook
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.179-185
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    • 2022
  • The feeding habits of Largehead hairtail Trichiurus japonicus was studied using 377 specimens collected in the Yellow Sea of Korea. The specimens ranged from 4.5 to 33.7 cm in Anal length (AL). T. japonicus consumed mainly a piscivore, such as Engraulis japonicus [percent index of relative importance (%IRI) =74.1%]. We calculated the trophic level as 3.84 for T. japonicus. Fishes were the main prey items for all seasons. The main fish prey during autumn and winter was E. japonicus, whereas those during spring and summer was Larimichthys polyactis. Fishes were the main prey items for all size groups (<15 cm, 15~20 cm, 20~25 cm, ≥25 cm). T. japonicus also showed size-related dietary shift from Spratelloides gracilis and E. japonicus to L. polyactis and T. japonicus. As the anal length of T. japonicus increased, the mean number of preys per stomach (mN/ST) and the mean weight of preys per stomach (mW/ST) tended to increased (One-way ANOVA, P<0.05). Seasonal and size-related shifts in dietary composition were investigated by PERMANOVA analysis, which showed significant variations among size classes and seasons.

Changes in Mental Health Status of Patients in the Community Treatment Center during the Quarantine Period (생활치료센터 환자의 격리 치료 기간 중 정신건강 상태 변화)

  • Jeong-Wook Seo;Jeonga Yoo;Jin-Yong Jun;Jiho Lee
    • Health Policy and Management
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    • v.34 no.3
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    • pp.293-308
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    • 2024
  • Background: Assessing the change in mental health status of quarantined patients in community treatment centers at the time of admission and discharge, and inferring the influencing factors. Methods: The study was conducted on a sample of 1,941 quarantined patients from three community treatment centers. Changes in anxiety, psychological distress, post-traumatic stress, depression, and self-harm ideation between admission and discharge were categorized as either "improved" or "worsened." Inference was performed to determine the probability of worsening in mental health status. Results: The mental health status of quarantined patients, such as anxiety and depression, was relatively higher than that of the general population. Anxiety (84.3% improved) and psychological distress (79.0% improved) were reduced during quarantine treatment. However, some patients continued to experience moderate to severe levels of anxiety (11.2%) and psychological distress (11.0%) at discharge. As for depression, the depression of moderate or higher level was increased at the time of discharge (28.7%→36.7%) compared to admission. The deterioration of anxiety and psychological distress was found to be the most significant factor influencing the worsening of depression at discharge (odds ratio [OR] for anxiety deterioration, 2.04; OR for psychological distress deterioration, 3.56). These effects were also observed similarly in post-traumatic stress and self-injury ideation. Conclusion: Improving anxiety and psychological distress among quarantined patients in community treatment centers can reduce the worsening of post-traumatic stress, depression, and self-injury ideation at the time of discharge. These findings provide evidence for the need for active mental health management from the initial stages of quarantine treatment.

Dose Planning of Forward Intensity Modulated Radiation Therapy for Nasopharyngeal Cancer using Compensating Filters (보상여과판을 이용한 비인강암의 전방위 강도변조 방사선치료계획)

  • Chu Sung Sil;Lee Sang-wook;Suh Chang Ok;Kim Gwi Eon
    • Radiation Oncology Journal
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    • v.19 no.1
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    • pp.53-65
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    • 2001
  • Purpose : To improve the local control of patients with nasopharyngeal cancer, we have implemented 3-D conformal radiotherapy and forward intensity modulated radiation therapy (IMRT) to used of compensating filters. Three dimension conformal radiotherapy with intensity modulation is a new modality for cancer treatments. We designed 3-D treatment planning with 3-D RTP (radiation treatment planning system) and evaluation dose distribution with tumor control probability (TCP) and normal tissue complication probability (NTCP). Material and Methods : We have developed a treatment plan consisting four intensity modulated photon fields that are delivered through the compensating tilters and block transmission for critical organs. We get a full size CT imaging including head and neck as 3 mm slices, and delineating PTV (planning target volume) and surrounding critical organs, and reconstructed 3D imaging on the computer windows. In the planning stage, the planner specifies the number of beams and their directions including non-coplanar, and the prescribed doses for the target volume and the permissible dose of normal organs and the overlap regions. We designed compensating filter according to tissue deficit and PTV volume shape also dose weighting for each field to obtain adequate dose distribution, and shielding blocks weighting for transmission. Therapeutic gains were evaluated by numerical equation of tumor control probability and normal tissue complication probability. The TCP and NTCP by DVH (dose volume histogram) were compared with the 3-D conformal radiotherapy and forward intensity modulated conformal radiotherapy by compensator and blocks weighting. Optimization for the weight distribution was peformed iteration with initial guess weight or the even weight distribution. The TCP and NTCP by DVH were compared with the 3-D conformal radiotherapy and intensitiy modulated conformal radiotherapy by compensator and blocks weighting. Results : Using a four field IMRT plan, we have customized dose distribution to conform and deliver sufficient dose to the PTV. In addition, in the overlap regions between the PTV and the normal organs (spinal cord, salivary grand, pituitary, optic nerves), the dose is kept within the tolerance of the respective organs. We evaluated to obtain sufficient TCP value and acceptable NTCP using compensating filters. Quality assurance checks show acceptable agreement between the planned and the implemented MLC(multi-leaf collimator). Conclusion : IMRT provides a powerful and efficient solution for complex planning problems where the surrounding normal tissues place severe constraints on the prescription dose. The intensity modulated fields can be efficaciously and accurately delivered using compensating filters.

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Analytical Studies on Yield and Yield Components in Barley (대맥의 수량 및 수량구성요소에 관한 해석적 연구)

  • Chung-Yun Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.18
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    • pp.88-123
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    • 1975
  • To obtain useful fundamental informations for improving cultural practices of barley, an investigation was made on the influences of different fertilizer level and seeding rate as well as seeding date on yield and yield components and their balancing procedure using barley variety Suwon # 18, and at the same time, 8 varieties including Suwon # 18 were also tested to clarify the varietal responses in terms of their yield and yield components under different seeding date at Crop Experiment Station, Suwon, during the period of 1969 and 1970. The results obtained were summarized as follows; 1. Days to emergence of barley variety Suwon # 18 at Suwon, took 8 to 19 days in accordance with given different seeding date (from Sept. 21 to Oct. 31). Earlier emergence was observed by early seeding and most of the seeds were emerged at 15$0^{\circ}C$ cumulated soil temperature at 5cm depth from surface under the favorable condition. 2. Degree of cold injury in different seeding date was seemed to be affected by the growth rate of seedlings and climatic condition during the wintering period. Over growth and number of leaves less than 5 to 6 on the main stem before wintering were brought in severe cold damage during the wintering period. 3. Even though the number of leaves on the main stem were variable from 11 to 16 depending upon the seeding date. this differences were occurred before wintering and less variation was observed after wintering. Particularly, differences of the number of main stem leaves from September 21 to October 11 seeding date were occurred due to the differences of number of main stem leaves before wintering. 4. Dry matter accumulation before wintering was high in early seeded plot and gradually decreased in accordance with delayed seeding date and less different in dry matter weight was observed after wintering. However, the increment rate of this dry matter was high from regrowth to heading time and became low during the ripening period. 5. Number of tillers per $\m^2$ was higher in early seeding than late one and dense planting was higher in the number of tillers than sparse planting. Number of tillers per plant was lower in number and variation in dense planting, and reverse tendency was observed in sparse planting. By increasing seedling rate in early seeding date the number of tiller per plant was remarkably decreased, but the seeding rate didn't affect the individual tillering capacity in the late seeding date. 6. Seedlings were from early planting reached maximum tillering stage earlier than those from the late planting and no remarkable changes was observed due to increased seeding rate. However. increased seeding rate tends to make it earlier the maximum tillering stage early. 7. Stage of maximum tillering was coincided with stage of 4-5 main stem leaves regardless the seeding date. 8. Number of heads per $\m^2$ was increased with increased seeding rate but considerable year variation in number of heads was observed by increased fertilizer level. Therefore, it was clear that there is no difficulties in increasing number of heads per $\m^2$ through increasing both fertilizer level and seeding rate. This type of tendency was more remarkable at optimum seeding time. In the other hand, seeding at optimum time is more important than increasing seeding rate, but increasing seeding rate was more effective in late seeding for obtaining desirable number of heads per $\m^2$. 9. Number of heads per $\m^2$ was decreased generally in all varieties tested in late seeding, but the degree of decrease by late seeding was lower in Suwon # 18. Yuegi, Hangmi and Buheung compared with Suwon # 4, Suwon # 6, Chilbo and Yungwolyukak. 10. Highly significant positive correlations were obtained between number of head and tillers per $\m^2$ from heading date in September 21 seeding, from before-wintering in October 1 seeding and in all growth period from October 11 to October 31 seeding. However, relatively low correlation coefficient was estimated between number of heads and tillers counted around late March to early April in any seeding date. 11. Valid tiller ratio varied from 33% to 76% and highest yield was obtained when valid tiller ratio was about 50%. Therefore, variation of valid tiller ratio was greater due to seeding date differences than due to seeding rate. Early seeding decreased the valid tiller ratio and gradually increased by delaying seeding date but decreased by increasing seeding rate. Among the varieties tested Suwon # 18, Hangmi, Yuegi as well as Buheung should be high valid tiller ratio not only in late seeding but also in early seeding. In contrast to this phenomena, Chilbo, Suwon # 4, Suwon # 6 and Yungwolyukak expressed low valid tiller ratio in general, and also exhibited the same tendency in late seeding date. 12. Number of grains per spike was increased by increasing fertilizer level and decreased by increasing seeding rate. Among the seeding date tested. October 21 (1969) and October 11 (1970) showed lowest number of grains per spike which was increased in both early seeding and late seeding date. There were no definite tendencies observed along with seeding date differences in respective varieties tested. 13. Variation of 1000 grain weight due to fertilizer level applied, seeding date and seeding rate was not so high as number of grains per spike and number of heads per $\m^2$, but exhibited high year variation. Increased seeding rate decreased the 1000 grain weight. Among the varieties tested Chilbo and Buheung expressed heavy grain weight, while Suwon # 18, Hangmi and Yuegi showed comparatively light grain weight. 14. Optimum seeding date in Suwon area was around October 1 to October 11. Yield was generally increased by increasing fertilizer level. Yield decrease due to early seeding was compensated in certain extent by increased fertilizer application. 15. Yield variations due to seeding rate differences were almost negligible compare to the variations due to fertilizer level and seeding date. In either early seeding or law fertilizer level yield variation due to seeding rate was not so remarkable. Increment of fertilizer application was more effective for yield increase especially at increased seeding rate. And also increased seeding rate fairly compensated the decrease of yield in late seeding date. 16. Optimum seeding rate was considered to be around 18-26 liters per 10a at N-P-K=10.5-6-6 kg/10a fertilizer level considering yield stabilization. 17. Varietal differences in optimum seeding date was quite remarkable Suwon # 6, Suwon # 4. Buheung noted high yield at early seeding and Suwon # 18, Yuegi and Hangmi yielded higher in seeding date of October 10. However, Buheung showed late seeding adaptability. 18. Highly significant positive correlations were observed between yield and yield components in all treatments. However, this correlation coefficient was increased positively by increased fertilizer level and decreased by increased seeding rate. Significant negative correlation coefficients were estimated between yield and number of grains per spike, since increased number of heads per m2 at the same level of fertilizer tends to decrease the number of grains per spike. Comparatively low correlation coefficients were estimated between 1000 grain weight and yield. 19. No significant relations in terms of correlation coefficients was observed between number of heads per $\m^2$ and 1000 grain weight or number of grains per head.

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Dental and skeletal characteristics and growth in Class III malocclusion between skeletal ages of 10 and 14 (10세에서 14세 사이 III급 부정교합자의 치아안면두개의 형태학적 특성에 대하여)

  • Min, Seungki;Lee, Young-Jun;Park, Young-Guk;Rothstein, Ted
    • The korean journal of orthodontics
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    • v.33 no.6 s.101
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    • pp.419-435
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    • 2003
  • The purpose of this study was to evaluate growth changes and skeletal characteristics of Korean children with Class III malocclusions from 10 to 14 years of skeletal age. Radiographs of 60 children with Class III malocclusion and 60 normal controls were assessed. Both groups were subdivided into 6 samples according to sex and skeletal age. Skeletal age was assessed using handwrist X-rays using the Greulich and Pyle norms. The Krogman-Walker plane (occipitale-maxillon) through Sella was used as a reference plane in this study with x-axis perpendicular to the x-axis. Sir Student t-tests were conducted to compare the control group with the Class III group according to each gender a:nd age. The characteristics of Class III malocclusion group compared to the control group included shorter anterior and posterior cranial base, shorter and retrusive maxilla, forger mandible, increased molar-incisor distance, retroclined lower incisors, labially proclined upper incisors, and anteriorly located mandibular molar, smaller upper and middle facial depth, and larger lower facial depth. Landmarks representing facial depth, size of maxilla and mandible, and their AP relationship including anterior facial height indicate that growth characteristic was determined early in life. But growth Pattern of cranial base and some of the dental landmarks showed progressive divergence between Control and Class III groups with age. The position of the posterior border of the mandible was found to be significantly forward in both females and males by the age of 14 and at the anterior border in males and females at all ages. Hyperdivergent mandibular plane, changes in anterior segment of mandible, small anterior cranial base, and decrease in cranial base flexure was also noted.

Development of analytical method for determination of spinetoram residues in livestock using LC-MS/MS (LC-MS/MS를 이용한 축산물 중 Spinetoram 공정시험법 개발 및 검증)

  • Ko, Ah-Young;Kim, Heejung;Do, Jung Ah;Jang, Jin;Lee, Eun Hyang;Ju, Yun Ji;Kim, Ji Young;Chang, Moon-Ik;Rhee, Gyu-Seek
    • Analytical Science and Technology
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    • v.29 no.2
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    • pp.94-103
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    • 2016
  • An analytical method was developed to determine the amount of spinetoram (spinetoram J and spinetoram L) in livestock samples. The spinetoram was extracted with acetonitrile and purified through a primary secondary amine (PSA) sorbent. The spinetoram residues were then quantified and confirmed using a liquid chromatography–tandem mass spectrometer (LC-MS/MS) in the positive ion mode using multiple reactions monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005-0.5 mg/kg) into a blank extract with r2 > 0.994. The limits of detection and quantification were 0.002 and 0.01 mg/kg, respectively. The recovery results of spinetram ranged between 81.9-106.4% at different concentration levels (LOQ, 10LOQ, 50LOQ, n=5) with relative standard deviations (RSDs) less than 10%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL40, 2003). An interlaboratory study was conducted to validate the method. The proposed analytical method proved to be accurate, effective, and sensitive for spinetoram determination. The method will be used as an official analytical method in Korea.

Spatial Distribution of Pigment Concentration Around the East Korean Warm Current Region Derived from Satellite Data - Satellite Observation in May 1980 - (위성원격탐사에 의한 동한난류 주변 해역의 색소농도 공간적 분포 -1980년 5월 관측을 중심으로 -)

  • Kim Sang Woo;Saitoh Sei-ich;Kim Dong Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.3
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    • pp.265-272
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    • 2002
  • Spatial distribution of Phytoplankton Pigment Concentration (PPC) and Sea Surface Temperature (SST) around the East Korean Warm Current (EKWC) was described, using both Coastal Zone Color Scanner (CZCS) images and Advanced Very High Resolution Radiometer (AVHRR) images in May, 1980. Water mass in this region can be classified into five categories in the horizontal profile of PPC and SST, nLw (normalized water-leaving radiance) images: (1) coastal cold water region associated with concentrations of dissolved organic material or yellow colored substances and suspended sediments, (2) cold water region of thermal frontal occurred by a combination of phytoplankton absorption and suspended materials, (3) warm water overlay region by the phytoplankton absorption than the suspended materials; (4) warm water region occurred by the low phytoplankton absorption, and (5) offshore region occurred by the high phytoplankton absorption. In particular, the highest PPC (>2.0 mg/m^3) area appeared in the CZCS and AVHRR images with a band shaped distribution of the thermal front and ocean color front region, which is located the coastal cold waters alonB western thermal front of the warm streamer of the EKWC. In this region, the highest PPC occurred by a combination of the high absorption of the phytoplankton (443 nm) and highest reflectance of suspended materials (550 nm). Another high PPC ($\simeq$$6\;mg/m^3$) appeared in the warm water overlay region inside warm streamer. High phytoplankton pigment concentration of this region was corresponding to the short wavelength of 443 nm, which represented phytoplankton absorption of the CZCS image.

Detection of Multidrug Resistance Using Molecular Nuclear Technique (분자핵의학 기법을 이용한 다약제내성 진단)

  • Lee, Jae-Tae;Ahn, Byeong-Cheol
    • The Korean Journal of Nuclear Medicine
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    • v.38 no.2
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    • pp.180-189
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    • 2004
  • Although the outcome of cancer patients after cytotoxic chemotherapy is related diverse mechanisms, multidrug resistance (MDR) for chemotherapeutic drugs due to cellular P-glycoprotein (Pgp) or multidrug-resistance associated protein (MRP) is most important factor in the chemotherapy failure to cancer. A large number of pharmacologic compounds, including verapamil, quinidine, tamoxifen, cyclosporin A and quinolone derivatives have been reported to overcome MDR. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are available for the detection of Pgp and MRP-mediated transporter. $^{99m}Tc$-MIBI and other $^{99m}Tc$-radiopharmaceuticals are substrates for Pgp and MRP, and have been used in clinical studies for tumor imaging, and to visualize blockade of PgP-mediated transport after modulation of Pgp pump. Colchicine, verapamil and daunorubicin labeled with $^{11}C$ have been evaluated for the quantification of Pgp-mediated transport with PET in vivo and reported to be feasible substrates with which to image Pgp function in tumors. Leukotrienes are specific substrates for MRP and $N-[^{11}C]acetyl-leukotriene$ E4 provides an opportunity to study MRP function non-invasively in vivo. SPECT and PET pharmaceuticals have successfully used to evaluate pharmacologic effects of MDR modulators. Imaging of MDR and reversal of MDR with bioluminescence in a living animal is also evaluated for future clinical trial. We have described recent advances in molecular imaging of MDR and reviewed recent publications regarding feasibility of SPECT and PET imaging to study the functionality of MDR transporters in vivo.