• 제목/요약/키워드: A. indica

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Analysis of Components in the Parts of Opuntia ficus indica from Shinan Korea (신안산 손바닥선인장 부위별 성분 분석)

  • Cho, In-Kyung;Jin, Seong-Woo;Kim, Yong-Doo
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.742-746
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    • 2009
  • The proximate compositions of Opuntia ficus indica fruits from Shinan were moisture 71.18%, crude protein 1.69%, crude ash 0.93%, crude fat 0.55%, crude fiber 1.54% and 24.11% nitrogen free extracts. And the proxmate compositions of Opuntia ficus indica stem were moisture 74.85%, crude protein 3.48%, crude ash 0.54%, crude fat 0.48%, crude fiber 2.58% and 18.07% nitrogen free extracts. The crude protein and crude fiber content of Opuntia ficus indica stem from Shinan were higher than those of fruit. The contents of fructose, sucrose and glucose were higher than maltose. As the result of organic acid, the content of citric acid was higher than malic acid and tartaric acid. As a result of mineral analysis, the contents of potassium was the highest among the minerals in Opuntia ficus indica from Shinan. Analysis of total amino acids in Opuntia ficus indica from Shinan. The contents of total amino acid of fruit and stem were 1,604.64 mg% and 3,245.98 mg% and the free amino acid contents of fruit and stem were 556.02 mg% and 1,101.35 mg%, respectively. The major components of total amino acids were glutamic acid, proline and threonine. The levels of total amino acids and free amino acids in Opuntia ficus indica stem were higher than those in its fruit.

Study on Biological Activities of Extracts for Cosmeceutical Development from Lagerstroemia indica L. Branch (화장품 개발을 위한 배롱나무(Lagerstroemia indica Linnaeus) 가지 추출물의 생리활성에 관한 연구)

  • Lee, Byung-Guen;Kim, Jong-Hyeop;Ham, Sang-Gyeong;Lee, Chang-Eeon
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.29-34
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    • 2014
  • The aim of the study was to examine the cosmetic and biological activity of Lagerstroemia indica L. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. Lagerstroemia indica L. branch was extracted with 70% acetone in water. In the result of DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging radical activity, acetone extract of Lagerstroemia indica L. branch were higher than 73% at the 50 ppm concentration. ABTS radical cation decolorization activity by acetone extract were higher than 78% at the 50 ppm. Both examine of DPPH and ABTS showed high antioxidative activities at the 50 ppm. In the result of nitrite scavenging ability, acetone extract were higher than 63% at the 50 ppm. Collagenase inhibition activity by extract were higher than 85% at the 50 ppm. Extract is showed high collagenase inhibition more than comparison group EGCG at all concentration. These results suggest that Lagerstroemia indica L. has a great potential as a cosmeceutical raw material as well as anti-oxidant and anti-inflammatory and collagenase inhibition activity.

Studies on the Pharmacological Actions and Biologically Active Components of Korean Traditional Medicines(VII) -On the Anticancer Macromolecular Substances from Duchesnea indica Herba- (한국전통전통 생약의 약리작용과 활성물질에 관한 연구(VII) -사매의 항암성 고분자물질-)

  • Lee, Ihn-Rhan;Hahn, Seung-Hye;Han, Yong-Nam;Jeong, Ga-Jin
    • Korean Journal of Pharmacognosy
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    • v.21 no.2
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    • pp.186-192
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    • 1990
  • This study was conducted to further purify and characterize a polysaccharide fraction from whole parts of Duchesnea indica (Andr.) Focke, which was previously reported to show an antitumor activity by us. Mild acid-treatment of the fraction by 5% acetic acid yielded three subtractions (Fr. A,B and C) on a Sephadex G-25. Fr. A was composed of uronic acid, hexose, pentose and phenolic substance, Fr. C mainly phenolic substance, and Fr. B contained hexose. All three fractions exhibited colony stimulating factor activity.

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Inhibitive Effect of Fuyuziphine isolated from Plant (Pittapapra) (Fumaria indica) on Spore Germination of Some Fungi

  • Pandey, M. B.;Singh, Ashok K.;Singh, Anil K.;Singh, U. P.
    • Mycobiology
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    • v.35 no.3
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    • pp.157-158
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    • 2007
  • The alkaloid fuyuziphine was isolated from the whole plant of Fumaria indica. It had inhibitive effect against spore germination of some plant pathogenic fungi (Collectotrichum sp., C. gloeosporioides, C. falcatum, Curvularia maculans, C. lunata, Erysiphe cichoracearum, Helminthosporium pennisetti, Oidium erysiphoides, Ustilago cynodontis, Alternaria chieranthi, A. mel-ongenae, A. brassicicola and A. solam). Curvularia lunata, Oidium erysiphoides, Alternaria brassicicola and A. solani did not germinate at 750 and 1000 ppm and Colletotrichum gloeosporioides, C. falcatum, Curvularia maculans were inhibited at 1000 ppm for 24 hr incubation. Germination of most fungi was significantly inhibited at $100{\sim}750ppm$.

Biochemical Studies on Hypoglycemic Agents (I) Effect of Azadirachta indica leaf extract

  • El-Hawary, Z.M.;Kholief, T.S.
    • Archives of Pharmacal Research
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    • v.13 no.1
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    • pp.108-112
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    • 1990
  • It was confirmed that extracts of some plants were used in folklore medicine as hypoglycemic agents. Of these plants is Azadirachta indica ("neem") which grows in tropical regions. The present study deals with biochemical effects of the "neem" leaf water extract given orally to experimental aminals, especially the hypoglycemic characteristics. Normals as well as alloxan diabetic rats have been used in this work. The results showed that the "neem" leaf extract produced some hypoglycemia in normal rats when given in two doses, while in diabetic rats there was a decrease in blood sugar, but it could not alleviate the diabetic in body weight loss and high percentage mortality, especially with a high dose. It was observed increased with concomitant decrease in the liver fat as compared to normal levels. There was also a drop in liver proteins which was dose-related. The results were compared wiht those obtained with an oral hypoglycemic drug (Glibenclamide).glycemic drug (Glibenclamide).

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Studies on the Pharmacological Actions and Biologically Active Components of Korean Traditional Medicine (VI). -Tannins from Duchesnea indica- (한국 전통생약의 약리작용과 활성물질에 관한 연구(VI). -사매의 Tannin계 물질-)

  • Lee, Ihn-Rhan;Wee, Seung-Won;Han, Yong-Nam
    • Korean Journal of Pharmacognosy
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    • v.20 no.2
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    • pp.110-116
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    • 1989
  • This study was conducted to purify and characterize a polysaccharide fraction from whole parts of Duchesnea indica (Andr.) Focke, which was previously reported to show an antitumor activity by us. The plant was extracted with 5% ethanol for 3 days in the room temperature. The extract was dialyzed by 5% ethanol, and then concentrated by extraction with $excess\;_N-butanol$, followed by gel filtration named Fr. a and b. Each was composed of gallic acid, hexose, pentose, uronic acid and protein, indicating that both fractions were tannic polysaccharide containing protein. Heat treatment of them yielded gallic acid less polysaccharides. They showed a colony stimulating factor activity.

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Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten

  • Huh, Yoon-Hee;Ko, Young-Hwan
    • Journal of Applied Biological Chemistry
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    • v.50 no.4
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    • pp.221-226
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    • 2007
  • The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

Antimicrobial Activity of Natural Product Made by Opuntia ficus-indica var. saboten Against Salmonella spp. and Escherichia coli O157:H7 (백련초 (Opuntia ficus-indica var., saboten)의 Salmonella와 Escherichia coli O157 : H7에 대한 항균효과)

  • Kim, So-Hyun;Kwon, Nam-Hoon;Kim, J.Y.;Lim, J.Y.;Bae, W.K.;Kim, J.M.;Noh, K.M.;Hur, J.;Jung, W.K.;Park, K.T.;Lee, J.E.;Ra, J.C.;Park, Yong-Ho
    • Journal of Food Hygiene and Safety
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    • v.17 no.2
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    • pp.71-78
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    • 2002
  • With the incidence of antibiotic resistant bacteria there is increasing interest in natural products such as herb extract and probiotics to control antibiotic resistant bacteria. This study was focused on the determination of antimicrobial activity of Opuntia ficus-indica var. saboten against Salmonella enetrica serovar Enteritidis (S. enterifidis), S. enterica serovar Typhimurium (S. Typhimurium) DT 104 and Escherichia coli 0157:H7. Though bactericidal effect of 0. ficus-indica var. saboten was not observed, it had significant inhibitory activity against Salmonella spp. and E. coli O157:H7 on the Moulter Hinton agar containing its solution dissolved in deionized water. To investigate the antimicrobial activity in vivo, mice were challenged with 5. Typhimurium DT104 (3.7$\times$108 cfu/mouse) after pre-feeding 0. ficus-indica var. saboten solution. The fecal shedding of S. Typhimurium DT104 was more dramatically decreased and not detectable in feces and intestines 3 days after challenge in mice fed with 0. ficus-indica var. saboten. Antibody responses of the intestinal IgA were also significantly increased in mice fed with 0. ficus-indica var. saboten. These findings suggest that Opuntia ficus-indica var. saboten decreased the shedding of S. Typhimurium DT104 in vitro and also in the gastrointestinal tract in mice. In addition, administration of the product might enhance the mucosal immune response against S. Typhimurium DT 104. In conclusion, Opuntia ficus-indica var. saboten might be useful to control antibiotic resistant bacteria in vivo and in vitro.

Methanol Extract of Flacourtia indica Aerial Parts Induces Apoptosis via Generation of ROS and Activation of Caspases in Human Colon Cancer HCT116 Cells

  • Park, Ki-Woong;Kundu, Juthika;Chae, In Gyeong;Bachar, Sitesh Chandra;Bae, Jung-Woo;Chun, Kyung-Soo
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.17
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    • pp.7291-7296
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    • 2014
  • Different plant parts of Flacourtia indica have long been used in Ayurvedic medicine. Previous studies have demonstrated that the methanolic extract of F. indica possess anti-inflammatory properties. The present study was aimed at investigating the anticancer effects of methanol extract of Flacourtia indica (FIM) aerial parts in human colon cancer (HCT116) cells. Treatment of cells with FIM at a concentration of $500{\mu}g/ml$ for 24 hours significantly reduced cell viability and induced apoptosis, which was associated with the increased cytoplasmic expression of cytochrome c, activation of caspase-3, and the cleavage of poly-(ADP-ribose) polymerase. Incubation with FIM also inhibited the levels of Bcl-2, Bcl-xl and survivin, which are the markers of cell proliferation, whereas the expression of Bax remained unchanged. Treatment with FIM led to the generation of reactive oxygen species (ROS) in a concentration-dependent manner. Pharmacological inhibition of ROS generation by pretreatment of cells with N-acetyl cysteine abrogated FIM-induced apoptosis in HCT116 cells. Thus, these results demonstrate that FIM has anti-proliferative and pro-apoptotic effects in HCT116 cells and the effects are, at least in part, due to the ROS dependent activation of caspases.

Identification of Subspecies-specific STS Markers and Their Association with Segregation Distortion in Rice(Oryza sativa L.)

  • Chin, Joong-Hyoun;Kim, Jung-Hee;Jiang, Wenzhu;Chu, Sang-Ho;Woo, Mi-Ok;Han, Longzhi;Brar, Darshan;Koh, Hee-Jong
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.175-184
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    • 2007
  • Two subspecies, japonica and indica, have been reported in rice, which differ in several ecotypic traits. However, reproductive barriers in hybrid progenies between subspecies have been major obstacles in breeding programs using inter-subspecific hybridization. As the first step to elucidate the reproductive barriers, we developed subspecies-specific(SS) STS markers in this study. A total of 765 STS primers were designed through comparing DNA sequences at every $2{\sim}3$cM interval between japonica and indica rices, which are available at Web DBs such as IRGSP, NCBI, TIGR, and GRAMENE, and tested for subspecies-specificity using 15 indica and 15 japonica varieties of diverse origin. Of them, 67 STS markers were identified as SS STS markers and their subspecies-specificity scores were estimated. The SS markers were dispersed throughout the genome along chromosomes. Of them, 64 SS markers were mapped on an RIL population derived from a Dasanbyeo(indica)/TR22183(japonica) cross. Genomic inclination of RILs was evaluated based on the genotyping with different types of markers. Association test between markers and segregation distortion revealed that segregation distortion might not be the cause of generating SS markers. The SS markers will be applicable to estimate the genomic inclination of varieties or lines and to study the differentiation of indica and japonica, and ultimately to breed true hybrid rice varieties in which desirable characters from both subspecies are recombined.

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