• The Korean Journal of Malacology
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• v.29 no.4
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• pp.273-281
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• 2013

In order to evaluate how the effects of aquacultural environment, such as temperature, salinity, DO, SS, Chlorophyll-a, COD and nutritive salts on Cupped oysters, an investigational study was conducted between September to December of 2011. During the study, different intermediate cage farms on the surface of the sea were used to culture and compare the growth and survival rate of the Cupped oysters in different fishing environments. The different intermediate cage farms used were Wonsando-ri, Chang-ri, and Pado-ri. In January of 2012, these oysters were transplanted to a horizontal net farm in a mudflat until July of that year. The adaptation rates of the Cupped oysters were tested at differential exposure times at varying intertidal periods. Wonsando-ri showed the highest water temperature and Chlorophyll-a levels, while the salinity was found to be within range of stable conditions among all three intermediate cage farms. Once the Cupped oysters were at the horizontal net farm, the growth was measured at distinct tidal exposure time of 1, 3, and 5 hours, whereby the growth rate was highest at 3, 1, and 5 hours, respectively. In addition, the oysters cultivated in intermediate cage farms had longer shell lengths compared to shell heights, while oysters cultured in the horizontal net farm had larger shell heights than shell lengths.

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.197-202
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• 2016

This research reports the intersexuality of bivalves, such as Crassostrea gigas, Mytilus galloprovincialis, Rupiditapes philippinarum, Gomphina veneriformis and Barnea davidi discovered during the process of investigating the ecological health status of coastal waters of Korea. In intersex ovaries, the opposite germ cells were observed either individually or in groups in the interfollicular space and inside the oogenic follicle. Oocytes in the intersex testis were at the previtellogenic or initial vitellogenic stage. They were either scattered individually or in groups in the interfollicular space and inside the spermatogenic follicle. The intersexuality in C. gigas was 10.4% (n = 19/183), while female (12.2%, n = 6/49) exhibited a higher proportion than male (9.7%, n = 13/134). The intersexuality in M. galloprovincialis was 31.7% (n = 19/60), while female (36.4%, n = 12/33) exhibited a higher proportion than male (25.9%, n = 7/27). The intersexuality in R. philippinarum was 11.2% (n = 11/98), while male (16.7%, n = 7/42) exhibited a higher proportion than female (7.1%, n = 4/56). The intersexuality in G. veneriformis was 28% (n=30/107), while male (31.5%, n=17/54) exhibited a higher proportion than female (24.5%, n=13/53). The intersexuality in B. davidi was 18.4% (n = 7/38), while female (35.7%, n =5 /14) exhibited a higher proportion than male (8.3%, n = 2/24).

• Analytical Science and Technology
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• v.28 no.6
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• pp.361-369
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• 2015

The precise mechanism underlying the therapeutic efficacy of an extraction powder of Angelica gigas (AGE) for the treatment of degenerative osteoarthritis was investigated in primary cultured rabbit chondrocytes and in a monosodium-iodoacetate (MIA)-induced osteoarthritis rat model. The treatment with AGE (50 μg/mL) effectively inhibited NF-B activation. The anti-inflammatory mechanism was clarified by gelatin zymography and western blotting measurements of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities. The AGE (50 μg/mL) treatment significantly reduced MMP-9 activity. The constituents of AGE— decursinol, decursin, and decursinol angelate—were determined by LC-MS/MS after a 24 hr treatment of rabbit chondrocytes. The contents of the major products, decursin and decursinol angelate, were 3.62±0.47 and 2.14 ±0.36 μg/mg protein, respectively in AGE-treated (50 μg/mL) rabbit chondrocytes. An in vivo animal study on rats fed a diet containing 25, 50, and 100 mg/kg AGE for 3 weeks revealed a significant inhibition of the MMPs in the MIA-induced rat articular cartilage. The genetic expression of arthritic factors in the articular cartilage was examined by RT-PCR of collagen Type I, collagen Type II, aggrecan, and MMP (MMP3, MMP-9, MMP13). Specifically, AGE up-regulated the expression of collagen Type I, collagen Type II, and aggrecan and inhibited MMP levels at all tested concentrations. Collectively, AGE showed a strong specific site of action on MMP regulation and protected against the degeneration of articular cartilage via cellular regulation of MMP expression both in vitro and in vivo.

• Korean Journal of Food Science and Technology
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• v.51 no.4
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• pp.336-342
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• 2019

To elucidate structure-function relationship of polysaccharide from Angelica gigas, the AGE-2c-I was purified by two successive chromatography steps. AGE-2c-I showed a potent anti-complementary activity in a dose-dependent manner. AGE-2c-I with a molecular weight of 140 kDa comprised four monosaccharides and 13 glycosyl linkages, and strongly reacted with ${\beta}$-glucosyl Yariv reagent. For the fine structure analysis of AGE-2c-I, it was sequentially digested by exo-arabinofuranosidase and endo-galactanase. The results indicated that AGE-2c-I was a typical RG-I polysaccharide with side chains such as highly branched ${\alpha}$-arabinan, ${\beta}$-($1{\rightarrow}4$)-galactan and arabino-${\beta}$-3,6-galactan. To characterize the active moiety of AGE-2c-I, the anti-complementary activities of AGE-2c-I and its subfractions were assayed. It was observed that the anti-complementary activity of AGE-2c-I was due to the entire structure that resembled RG-I. In addition, arabino-${\beta}$-3,6-galactan side chain (GN-I) in AGE-2c-I probably plays a crucial role in the anti-complementary activity, whereas ${\alpha}$-arabinan side chain (AFN-I) consisting of 5-linked Araf and 3,5-branched Araf partially contributes to the activity.

• Journal of Life Science
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• v.31 no.11
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• pp.1004-1009
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• 2021

Angelica gigas Nakai (AGN) has been used in Korean herbal medicine for various pharmacological activities, such as to create antioxidant and skin whitening effects. Decursin and decursinol angelate of AGN extracts can be used as potential active drugs and cosmetic ingredients. This study investigated the possibility of topical delivery of AGN extracts using a manufactured emulsion system. Lotion was formulated by using Tefose^® and paraffin for the oil phase, Kolliphor RH 40 for the surfactant and solubilizing agent-which showed high solubility in water (0.82 mg/ml)-and a water phase with a carbomer. In vitro skin permeation of decursin and decursinol angelate was determined using a Strat-M^® membrane in Franz diffusion cells. Lotion samples as the experimental group (248.08±19.72 ug/cm²) significantly increased the permeation of decursin and decursinol angelate for up to 24 hr compared to the control group (119.18±19.23 ug/cm²). The permeability was also characterized by the flux (penetration rates) and Kp (permeability coefficient) values. The experimental group (17.20±1.23 ug/h/cm² and 5.73±1.39 cm/h^*10^-3) had higher flux and Kp than the control group (8.22±1.24 ug/h/cm² and 2.74±0.51 cm/h^*10^-3). Lotion with decursin and decursinol angelate of AGN extracts could be used for the topical application of drug and cosmetic products.

• Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.84-87
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• 2010

A peptide that inhibits HIV-1 protease was isolated from a hydrolysate of oyster (Crassostrea gigas) proteins digested with thermolysin. The peptide was using membrane filtration, gel permeation chromatography, ion exchange chromatography, and reverse-phase high performance liquid chromatography. Amino acid sequence of the peptide was determined to be Val-Phe-Glu-Leu. Chemically synthesized Val-Phe-Glu-Leu showed an $IC_{50}$ value of 106 ${\mu}M$.

• Korean Journal of Plant Resources
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• v.26 no.6
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• pp.701-708
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• 2013

To test the quality change of seedlings of three domestic medicinal plants raised in plug trays, a short term storage experiment was conducted. Seedlings were kept in growth chambers for two weeks at 4 or $8^{\circ}C$ temperature combined with 0 or $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. Quality of glasshouse-raised seedlings was assessed after two weeks of cold storage in the growth chamber and one week of acclimation in the greenhouse. After two weeks of storage in the growth chamber of Perilla frutescens var. acuta Kudo, plant height was the greatest in the treatment $8^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. Internode length of P. frutescens var. acuta Kudo was the greatest in the treatment of $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. After one week of acclimatization in a glasshouse, the growth and development, such as plant height, internode length and leaf size, were greater in the $8^{\circ}C$ combined with $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD than in the other treatments. After two weeks of storage in the growth chamber of Sophora tonkinensis, plant height increased more in the treatment of $4^{\circ}C$ than $8^{\circ}C$. After one week of acclimatization in a glasshouse, number of leaves did not change in the treatment of $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD, but it increased in the other treatments. Leaf width increased more under the dark than light condition. Leaf length did not observably change in any treatments. After two weeks of storage in the growth chamber, plant height of Angelica gigas Nakai was the greatest in the treatment of $8^{\circ}C$. Number of leaves was the greatest in the treatment of $8^{\circ}C$. Leaf growth was greater under dark than light condition. These results suggested that optimal storage environment was $8^{\circ}C$ combined with $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD for P. frutescens var. acuta Kudo, and $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD for S. tonkinensis and A. gigas Nakai. Hence, proper combination of temperature and PPFD were necessary for better storage, and acclimatization and growth, thereafter, of the plug seedlings of theses plant species.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.419-422
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• 2002

Korean angelica(Angelica gigas Nakai), a oriental medicinal plants is important and in great demand in Korea. This crop is a biennial which is to die after flower, so as could not harvest the root. A new variety 'Anpung' was developed from the selection of the local collecting population by medicinal crop team at NCES in 2001. This showed stable bolting during its cropping year and have high quality and yield potential. The color is greenish purple in stem and red purple in flower. It was longer in plant height and root length, thicker in root diameter, more leave and latheral roots comparing with the check variety 'Manchu'. The dry root was 72 g/plant showing heavier than check variety of 65 g/plant. It showed such low bolting rate as 0.5%, on the other hand 49.3% in landrace when it cultured by transplanting with heating nursery seedlings, and $0{\sim}15%$ with field nursery seedlings. The dry root yield of the variety was averaged 3,630 kg/ha out yielding the check variety by 11% at the three regional yield test from 1999 to 2001. Finally, 'Anpung' showed higher content of extracts up to 46.3% in 'Anpung' and 45% in check variety, decursin and decursinol contents was 3.48% and 2.79%, showing slightly higher than check variety.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.495-500
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• 2014

We examined chemical changes in oysters Crassostrea gigas and packing water that were sold after storage at 5, 10, and $20^{\circ}C$. The pH of oysters stored at $5^{\circ}C$ dropped to 5.81 after 10 days of storage, while that of oysters at $10^{\circ}C$ and $20^{\circ}C$ dropped to 5.37 after 8 days and to 5.04 after 4 days, respectively. The glycogen content of oysters stored at $5^{\circ}C$ decreased from 718.89 to 421.85 mg/100g during storage, while that of oysters at $10^{\circ}C$ decreased to 351.49 mg/100 g after 4 days. The turbidity and soluble protein in packing water increased slightly. The viable cell count of oysters did not exceed 6 log CFU/g after 10 days of storage at $5^{\circ}C$, but that of oysters at $10^{\circ}C$ did so after 8 days. Additionally, the viable cell count of packing water was lower than that of oysters. We performed a principal component analysis, where the first principal component (55.03%-57.24%) and second principal component (42.76%-44.97%) described most variation. The first principal component included the pH of oysters and packing water, and the glycogen content of oysters. A Pearson correlation between the first two principal components had a higher R value than that between other components. Freshness was evaluated using the pH of oysters and packing water, and glycogen. We found that soluble protein content was significantly associated with a lower pH and glycogen content.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.6
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• pp.876-882
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• 2015

The aim of this study was to investigate changes in the biochemical characteristics of the Pacific oyster Crassostrea gigas in response to boiling water bath ($85^{\circ}C$, $100^{\circ}C$) and autoclaving ($100^{\circ}C$) heat treatments for 5, 10, 15, and 20 min. The weight loss (7.6-9.5%) of oysters exposed to a $100^{\circ}C$ boiling water bath for 15 min was lower compared to those receiving no treatment. Oysters exposed to the $100^{\circ}C$ boiling water bath for 20 min lost 27.8% of their total weight. The $100^{\circ}C$ autoclaving treatment resulted in weight loss of 13.0-26.1%, with the highest weight loss occurring after 10 min of treatment (26.1%). Heat-treated oysters had significantly higher levels of crude lipid and crude protein relative to untreated oysters (P<0.05). In the heat-treated oysters, total amino acids (including proline and glutamic acid) were increased, but they were decreased by the 20-min $100^{\circ}C$ boiling water bath and autoclaving treatments. The fatty acid composition of oysters was not significantly affected by the water bath heat treatment, but oysters treated with $100^{\circ}C$ autoclaving for 20 min had decreased polyunsaturated fatty acids. Oysters exposed to the water bath heat treatment had significantly increased vitamin A content, but $100^{\circ}C$ autoclaving for 20 min was associated with reduced vitamin A content, as compared to all of the other heat treatments. Therefore, oysters were affected by heat treatment, undergoing metamorphosis and weight loss, but nutrient quality did not change significantly compared to controls, except with the 20-min $100^{\circ}C$ boiling water bath and autoclaving treatments.