• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.155-158
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• 2000

Angelica gigas Nakai 뿌리배양을 통한 decursinol angelate생산을 증대시키기 위하여 여러 가지 elicitor들을 처리하여 생산 증진을 도모하였다. 가장 효과적인 elicitor로는 hydrogen peroxide와 $CM-\;{\beta}\;-chitin$이었으며, 이때의 g당 생산되는 decursinol angelate 양은 각각 대조구에 비해 2.12, 1.62배 증대된 값이다.

• Molecular & Cellular Toxicology
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• 제5권2호
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• pp.153-159
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• 2009

In this study, we assessed the acute and subacute toxicity of Angelica gigas Nakai (A. gigas Nakai) extracts, which are comprised of decursin and decursinol angelate (D/DA) in rats. For the oral acute toxicity test, Sprague-Dawley (SD) male and female rats were gavaged with two doses of D/DA (200 and 2,000 mg/kg body weight) and then observed for any toxic symptoms for 2 weeks. The LD$_{50}$ value for the rats was greater than 2,000 mg/kg body weight for both male and female rats, which indicates that there were no toxic symptoms induced by doses of up to 2,000 mg/kg body weight. For the subacute toxicity study, rats were treated with D/DA at doses of 2 and 20 mg/kg body weight once a day for 30 days. There were no significant changes in body weight and food intake observed during the subacute toxicity study. In addition, no differences were observed between the control and treated groups when urinalysis was conducted or when hematology and biochemical parameters were evaluated. Finally, histopathological examination of the organs did not reveal any lesions in the control or treated groups. Taken together, these findings indicate that D/DA is safe and non-toxic.

• Plant Resources
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• 제6권3호
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• pp.183-187
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• 2003

Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica

• Fisheries and Aquatic Sciences
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• 제15권1호
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• pp.15-19
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• 2012

A new cyclic peptide (six-membered amino acid), gamakamide-E (L-Leu-L-Met (SO)-L-Me-Phe-L-Leu-D-Lys-L-Phe), was isolated as a strongly bitter tasting compound from cultured oysters, Crassostrea gigas. The molecular formula of $C_{43}H_{61}N_7O_8S$ was deduced from high resolution fast atom bombardment mass spectrometry (HR FAB-MS) ($[M+H]^+$ m/z 836.4356 ${\Delta}$= -2.4 mmu). Its unique structure including a hydantoin structure was firstly elucidated by nuclear magnetic resonance (NMR) analysis. Stereochemistries of constituent amino acids were determined by chiral high performanced liquid chromatography analysis of natural and synthesized peptides.

• Archives of Pharmacal Research
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• 제26권9호
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• pp.723-726
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• 2003

Five coumarins, isoimperatorin (1), pabulenol (2), isooxypeucedanin (3), oxypeucedanin hydrate (4) and osthol (5) were isolated from the MeOH extract of Angelica genuflexa in the course of searching for anti-platelet and anti-coagulant components from plants. Pabulenol (2) was isolated from A. genuflexa for the first time. The five compounds isolated from A. genuflexa, together with decursinol angelate (6), decursin (7) and nodakenin (8) from A. gigas were evaluated for their effects on platelet aggregation and blood coagulation. Compounds 2, 5, 6 and 7 were observed to be either equally effective or 2∼4 times more inhibitory than ASA in both arachidonic acid and U46619 ($TXA_2$ mimetic) induced platelet aggregations.

• 생약학회지
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• 제34권3호통권134호
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• pp.201-205
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• 2003

This paper is intended as an investigation of the analysis of high-performance liquid chromatography and the method of extraction of decursin and decursinol angelate in Angelica gigas roots. There are three kinds of extraction methods: distilled water, 50% EtOH and 100% EtOH. The condition of HPLC was obtained on a reversed-phase column $(Polarity\;dC_{18},\;4.6{\times}250 mm,\;5\;{\mu}m)$ using a phosphate buffer-acetonitrile-sodium lauryl sulfate as the mobile phase. Under these chromatographic conditions, UV detector was 230 nm, column temperature $30^{\circ}C$ and the speed of a current 1.0 ml/min, respectively. The results of extraction with distilled water, 50% EtOH and 100% EtOH in Angelica gigas roots were as follows. The concentrations of decursin and decursinol angelate were 182 and 153 ppm (distilled water), 3,142 and 2,547 ppm (50% EtOH) and 3,341 and 2,778 ppm (100% EtOH). There were high positive correlations between the concentrations of decursin and EtOH (r=0.8928, p<0.01) and decursinol angelate and EtOH (r=0.9009, p<0.01).

• 한국수산과학회지
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• 제51권6호
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• pp.647-655
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• 2018

This study collected basic data on two types of canned roasted oyster Crassostrea gigas. Oysters Crassostrea gigas were immersed at $105^{\circ}C$ for 6 min and then washed and dehydrated before pre-drying. Roasted oysters were prepared by baking boiled oysters at $140^{\circ}C$ for 20 min. The canned roasted oyster added with tomato sauce was prepared as follows. An aluminum can was filled with 50 g of roasted oyster and 40 g of mixed seasoning sauce, degassed at $90^{\circ}C$ for 3 min and vacuum-sealed using a double seamer under a 20 cmHg vacuum. The canned roasted oyster added with tomato paste sauce was prepared similarly by adding the same amount of tomato paste sauce instead of tomato sauce. Microbial growth, appearance, proximate composition, pH, volatile basic nitrogen (VBN), thiobarbituric acid (TBA) value, amino-N, salinity, color value, texture, free and total amino acids, and minerals were measured in the two products. A sensory evaluation indicated that the canned roasted oyster added with tomato paste sauce had preferable characteristics over the canned roasted oyster sauce added with tomato sauce.

• Biomolecules & Therapeutics
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• 제19권1호
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• pp.33-37
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• 2011

Decursin was purified from Angelica gigas Nakai, and its effects on the human Kv1.5 (hKv1.5) currents were recorded in mouse fibroblasts ($Ltk^-$ cells) by whole-cell patch-clamp technique. Decursin inhibited hKv1.5 current in a concentration-dependent manner, with an $IC_{50}$ value of $2.7\;{\mu}M$ at +60 mV. Decursin accelerated the inactivation kinetics of the hKv1.5 channel, and slowed the deactivation kinetics of the hKv1.5 current, resulting in a tail crossover phenomenon. Also, decursin inhibited the hKv1.5 current in a use-dependent manner. These results strongly suggest that decursin is a kind of open-channel blocker of the hKv1.5 channel.

• Animal cells and systems
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• 제12권4호
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• pp.305-311
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• 2008

Age and growth of the mudskipper, Scartelaos gigas were investigated using the second actinost bone of the pectoral girdle, based on an analysis of 560 individuals collected from the mud flats in south west Korea. Specimens were collected semimonthly from March to September of 2003. Actinost and ring radii of each ring group showed a direct one-to-one relationship, with ring radius increasing in tandem with actinost radius. Monthly change in the marginal indices showed that ring formation occurred between May and July(mainly June), being supported by the monthly change in the gonadosomatic(highest in June) and hepatosomatic(lowest in July) indices. Because the species has not been found since November, when air temperature was less than about $10^{\circ}C$, it is thought to enter to hibernation. Therefore, it was suggested that the two new actinost rings may appear a year: one during the spawning season(May-July) and another during hibernation(since November). Although the growth of S. gigas must be limited to short periods each year from March to April and from August to October, its growth rate was considerably fast. The von Bertalanffy growth parameters of S. gigas were $TL_{\infty}$=179.36 mm, K=0.78 $year^{-1}$, $t_0$=-0.7762, and age-length key were $TL_1$=134.3 mm, $TL_2$=158.6 mm, $TL_3$=169.1 mm, $TL_4$=175.0 mm.

• 해양환경안전학회지
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• 제23권6호
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• pp.684-689
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• 2017

본 연구는 참굴(Crassostrea gigas)의 패각운동을 이용하여, 연안역에서 발생하는 저염수에 대한 조기경보가능성을 살펴보았다. 30 psu와 20 psu에서 패각운동은 각각 $7.32{\pm}3.21$회/hr와 $7.11{\pm}3.90$회/hr였으며, 파형과 횟수는 차이가 없었다(t-test, p>0.001). 하지만 10 psu와 5 psu에서는 모든 개체가 폐각상태를 지속하였다. 수온과 염분의 복합실험결과, Group 1(수온 $15^{\circ}C$ ${\times}$ 염분 15 psu)은 20~30 psu에서 보인 패각운동 후(약 2~3시간), 장시간 폐각을 하였다. Group 2(수온 $30^{\circ}C$ ${\times}$ 염분 15 psu)에서는 Group 1의 패각 개폐운동보다 더 빠르고 자주 나타나, 참굴의 생리적인 위기상황에 대한 신호를 나타내었다. 따라서 이러한 파형은 하계 저염수 출현 시 나타낼 수 있는 조기경보 신호로 충분히 활용될 수 있을 것으로 보인다.