• Journal of Korean Society of Forest Science
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• v.90 no.3
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• pp.249-256
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• 2001

The objective of this study was to analyze characters of the vegetation structure, vegetation-environment relationships of Kyeryongsan forest community. Fifty plots were sampled in the valley forest of Donghaksa, Gabsa, Sanghasinri and Shinwonsa regions in Kyeryongsan. Field survey was carried out in August of 1999 to examine a vegetation type and measure a diameter. The classification by TWINSPAN, the DCCA ordination, and structure of stand were used to analyze data. The results of this study are as follows : 1. With the classification by TWINSPAN, the community was categorized into four groups, such as Carpinus laxiflora, Quercus variabilis-Quercus serrata, Zelkova serrata-Celtis jessoensis and Carpinus cordata communities. 2. The dominant species in Carpinus laxiflora community were Carpinus laxiflora, Quercus variabilis, Quercus serrata, Pinus densiflora and Styrax japonica. The dominant species in Quercus variabilis-Quercus serrata community were Quercus variabilis, Quercus serrata, Styrax japonica, Castanea crenata and Platycarya strobilacea. The dominant species in Zelkova serrata-Celtic jessoensis community were Quercus serrata, Castanea crenata, Lindera erythrocarpa, Zelkova serrata and Styrax japonica. The dominant species in Carpinus cordata community were Carpinus cordata, Carpinus laxiflora, Sapium japonicum, Cornus controversa and Zelkova serrata. 3. The results of the correlation between Kyeryongsan valley forest community and environmental factors by DCCA ordination method are as follows; The Carpinus laxiflora community was found in low elevation and steep slope area. The Quercus variabilis-Quercus serrata community was found in low elevation and lower slope area. The Zelkova serrata-Celtis jessoensis community was found in high elevation and lower slope area. The Carpinus cordata community was found in high elevation and midium slope area.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.2
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• pp.87-93
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• 2003

Litterfall and litter decomposition represent a major contribution to the carbon and nutrient inputs in a forest ecosystem. We measured litterfall quantity and nutrient dynamics in decomposing litter for two years at the Kwangneung broadleaf natural forest (DK site) in Korea. Litterfall was collected in circular littertraps (collecting area : 0.25 $m^2$) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30 cm ${\times}$ 30 cm nylon bags with 1.5 mm mesh size. Total annual litterfall was 5,627 kg/ha/yr and leaf litter accounted for 61 % of the litterfall. The leaf litter quantity was highest in Quercus serrata, fallowed by Carpinus laxiflora and C. cordata, etc., which are dominant tree species in the site. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. Lower mass loss rates of Q. serrata litter may be attributed to the difference of substrate quality such as lower nutrient concentrations compared with the other litter types. Nutrient concentrations (N, P, Mg) of three litter types except for potassium (K) increased compared with initial nutrient concentrations of litter over the study period. Compared with Q. serrata litter, nutrients (N, P, K, Ca, Mg) in C. laxiflora and C. cordata litter were released rapidly. The results suggest that litter mass loss and nutrient dynamic processes among tree species vary considerably in the same site conditions.

• Korean Journal of Veterinary Service
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• v.33 no.2
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• pp.185-197
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• 2010

This study was conducted to evaluate the alleviative effects of activated charcoal (AC) and Houttuynia cordata (HC) singly or in combination in broiler chickens during aflatoxicosis. Activated charcoal (1% or 0.5%) and H. cordata (1% or 0.5%) were mixed into the diets for the ability to reduced the deleterious effects of 2.4mg total aflatoxin $(AFB_1)kg^{-1}$ diet on growing broiler chickens from 1 to 21 days of age. A total of 160 1-day-old (Hyline Variety Brown) broiler chicks were housed in eight treatment groups [Control, $AFB_1$, AC 1%, HC 1%, $AFB_1$ plus AC 1% plus HC 1%, $AFB_1$ plus AC 1% plus HC 0.5%, $AFB_1$ plus AC 0.5% plus HC 1%, $AFB_1$ plus AC 0.5% plus HC 0.5%] each consisting of 20 chicks. Compared to control, 2.4mg $AFB_1$ alone treatment group significantly decreased body weight gains of chickens. The addition of mixed AC 1% and HC 1% including 6, 7 groups to the 2.4mg $AFB_1$-containing diet moderately reduced the adverse effects of $AFB_1$ on performances of chickens. The chickens consuming 2.4mg $AFB_1$ plus AC 0.5% and HC 0.5%-containing diet showed very slightly reduced the adverse effects on investigated parameters compared to the $AFB_1$ only treated group. Also, the single addition of AC or HC to the $AFB_1$-free diet had no adverse effects in chickens. These results suggest that AC and HC mixed can reduced the aflatoxicosis in broilers and may be contribute to a solution of the aflatoxicosis problem in poultry production.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.202-207
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• 2004

This study was performed to investigate the inhibitory effect of the water-extract from fermented compositions containing Inonotus obliquus with added Houttuynia cordata on the growth of either human AGS gastric and HCT-15 colon cancer cells or NIH3T3 normal mouse fibroblast cells. Cytotoxic activity on cancer cells was investigated by viable cell count, MTT assay and morphological observation. Mixtures of Inonotus obliquus with added Houttuynia cordata were fermented at $30{\sim}37^{\circ}C$, $50{\sim}60%$ humidity for 30 days, extracted with water, freeze dried, powered, and then dissolved in water for the experiment. In MTT assay, the fermented compositions exhibited inhibitory effects of 13, 25, 40, 67 and 78% for AGS and 22, 40, 50, 69 and 76% for HCT-15 at 0.16, 0.4, 0.8, 1.6 and 4.0 mg/ml, respectively. However, normal NIH3T3 cells were exhibited 86% survival under the same experimental condition. Fermented compositions showed highly inhibitory effect against human cancer cell line HCT-15 and AGS, but not on normal cell line NIH3T3.

• Journal of Korean Society of Forest Science
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• v.86 no.2
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• pp.214-222
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• 1997

Employing the relev$\acute{e}$ method of Muller-Dombois & Ellenberg and Braun-Blanquet, 100 plots were sampled in the Quercus mongolica community of Mt. Odae, Mt. Chumbong and Mt. Jungwang in Kangwon-do. To analyze the data, phytosociological method and TWINSPAN were used. 1. Quercus mongolica forests were recognized as Quercus mongolica-Acer pseudosieboldianum community group, and were classified into Quercus mongolica-Carpinus cordata community, Quercus mongolica-Acer pseudosieboldianum typical community, Quercus mongolica-Lespedeza maximocoiczii community and Quercus mongolica-Abies nephrolepis community by phytosociological method. Quercus mongolica-Carpinus cordata community was subdivided into Acer mandshuricum subcommunity and typical subcommunity. 2. According to the classification of TWINSPAN, it was categorized into five groups, such as Quercus mongolica-Acer mandshuricum community, Quercus mongolica-Carpinus cordata community, Quercus mongolica-Acer pseudosieboldianum community, Quercus mongolica-Lindera obtusiloba community, and Quercus mongolica-Abies nephrolepis community. 3. Since the results of phytosociological community classification, which identify five groups of the community, were consistent with that of TWINSPAN, it proved that these two methods could be complement when one do a community classification.

• Food Science and Preservation
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• v.17 no.6
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• pp.835-846
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• 2010

The manufacture of beverages prepared using hot-water extracts of Polygonatum odoratum var. pluriflorum, Houttuynia cordata, and Lycium chinensis was statistically optimized using response surface methodology. The total polyphenol content, electron donation activity, and reducing power of optimized beverages were measured to evaluate antioxidant properties. The coefficients of determination, the $R_2$ values, were 0.9887~0.9957 for color, taste and overall acceptability. Analysis of variance showed that the model fit was a statistically significant (p<0.05). The optimized compositions of processed beverages were: Polygonatum odoratum var. pluriflorum extract, 2.15%; Houttuynia cordata extract, 1.74%; and Lycium chinensis extract, 0.19% (all w/w). Total polyphenol content, electron donation activity, and reducing power of beverages of optimized composition were 3.61 mg/L, 23.78%, and 0.26, respectively.

• Journal of Radiation Industry
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• v.5 no.3
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• pp.273-277
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• 2011

This study evaluated the cytotoxicity and anti-inflammatory activities of natural herbal extracts including Houttuynia cordata and Eucommia ulmoides against human mast cell (HMC-1). Houttuynia cordata (HC) and Eucommia ulmoides(EU) were extracted with distilled water (at $75^{\circ}C$) and then freeze-dried for 5 days. Finally, the mixture of HC and EU were sterilized by ${\gamma}$-rays irradiation. Cytotoxicity of the mixture against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory mediator cytokines such as TNF-${\alpha}$, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showed that mixture of HC and EU had no cytoxicity and reduced TNF-${\alpha}$, IL-6, IL-8 response on HMC-1 cells.

• Korean Journal of Medicinal Crop Science
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• v.21 no.1
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• pp.20-26
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• 2013

This study was conducted to improve the managing and storing methods of the seeds of Aralia cordata var. continentalis (Kitagawa) Y.C.Chu, to examine the viability and the germination ability of seeds with different storing conditions and methods, and to develop new ways to propagate and have better healthy seedling. Therefore, the germination rate, days required for germinating seeds, and early growth responses of Aralia cordata var. continentalis (Kitagawa) Y.C.Chu were investigated with different storing temperatures, durations and methods. The germination rate was higher in stratified storage than that in dry storage condition. The highest germination rate was with outdoor temperature at 30 days after stratified storage. The days required for germinating seeds were less than 10 days with the treatment of $25^{\circ}C$ and outdoor temperature in stratified storage. In dry condition, they were shorter with $4^{\circ}C$ and $25^{\circ}C$ than those with $-20^{\circ}C$ and outdoor temperature. Leaf number of seedling was higher in stratified storage compared to that in dry condition, while it was not clearly different according to storage temperatures and durations. Leaf length and leaf width of seedling was not difference among the treatment of storage methods, temperatures, and durations. Stem length of seedling was higher in stratified storage than those in dry condition, while root length was not clearly different among the treatments. It would be assumed that temperatures, methods and durations of storage could affect much to the germination rate and the early seedling growth response.

• The Korean Journal of Ecology
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• v.19 no.6
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• pp.563-573
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• 1996

Macroinvertebrate contribution to the leaf litter breakdown of Carpinus cordata was estimated at headwater streams at Mt. Jumbong (38°03'N, 128°25'E) during spring and winter spring by using two types of litter bag. Coarse-mesh bags with 10 g of leaf letter were placed in a 1st-order stream in April (the spring experiment) and December 1995 (the winter-spring experiment). Fine-mesh bags with 5 g of leaf letter were placed in a nearby 3ed-order steam. The breakdown of Carpinus in coarse-mesh bags was rapid, and, in terms of season, leaf litter processed rapidly during spring. daily mass loss rates of leaf litter (-k±1 SE) were highest for coarse-mesh bags in the spring experiment (-0.0429±0.0048), followed by coarse-mesh bags in the winter-spring (-0.0146±0.0014), fine-mesh bags in the spring (-0.0078±0.0004), fine-mesh bags in the winter-spring experiment (-0.0054±0.0005). Macroinvertebrate contribution to the litter breakdown was estimated by the difference of % leaf letter remaining between coarse -mesh bage and fine-mesh bags. Although shredders were more abundant during the winter-spring, their contribution was greater during the spring (50%) than the winter-spring (22∼33%). This result appeared to be due to the change in the chemical composition of leaf letter during processing, and to the seasonal growth patterns of major shredder taxa.

• Journal of Life Science
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• v.25 no.3
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• pp.329-335
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• 2015

This study was performed to determine the optimal ratio of Petasites japonicus, Luffa cylindrica, and Houttuynia cordata, all of which are supposed to have anti-respiratory disease effects, such as against rhinitis. The experiment incorporated a mixture design and included 12 experimental points with center replicates for three different independent variables (Petasites japonicus 30~70%; Luffa cylindrica 10~30%; and Houttuynia cordata 10~30%). Based on this design, the mixture was extracted in hot water at 121℃ for 45 min and anti-allergy and anti-microbial activities were observed. The response surface and trace plot described for the anti-allergy activity showed Petasites japonicas was a relatively important factor. The correlation coefficient (R²) value 82.10% for the inhibition effect of degranulation was analyzed by the regression equation. The analysis of variance showed the model fit was statistically significant (p<0.05). The optimal ratio of the mixture was Petasites japonicus 0.75%, Luffa cylindrica 0.11%, and Houttuynia cordata 0.14%. The anti-microbial activity for each extraction of the mixture was valid on gram-positive, such as Staphylococcus aureus (KCCM 40881) and Staphylococcus epidermidis (KCCM 35494), while it was less effective on gram-negative, such as Escherichia coli (KCCM 11234) and Pseudomonas aeruginosa (KCCM 11328).