• 한국학교ㆍ지역보건교육학회지
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• 제16권2호
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• pp.101-114
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• 2015

Objectives: Find out how it affects anxiety situations and self-esteem targeting the critical period of development of self-esteem in the lower grades in elementary school based on laughter therapy in having a positive influence on physical health promotion and positive emotional cultivation. And to verify the effects of laughter activity was to provide preliminary data on mental health education and guidance of school life. Methods: By targeting Ulsan Y elementary school, second grade 7 class, 191 students conducted test self-esteem and anxiety. Homogeneous group of experimental and control groups were selected in each one class. Classified as experimental group carried out laughter activity programs and control group did not carried out. Also the separation of the application program before and after, then it were performed preliminary-post-further as same checklist. Program coverage period is 12 weeks and 30 minutes per session was conducted twice a week. Differences between the experimental group and the comparison group has conducted the independent samples t-test, and if the difference between the experimental group and the comparison group were verified by performing a variance analysis(Oneway ANOVA) and post multiple comparisons. Results: Analysis of the experimental group decreased anxiety in the post-test than the pre-test as a whole, in particular, sub-areas of concern, shame and shyness was shown to be effective, and decision of the difficulty was not significant by insignificant reduction. In addition, self-esteem was increased in overall score. The score of collective self-esteem and School life self-esteem were more increased, and domestic self-esteem was not significant by slight increases. In contrast, the comparison group showed no significant difference in all areas of anxiety and self-esteem of pre-test and post-test. Conclusions: In experiments on self-esteem and anxiety of lower grade in elementary school through laughter activity program, comparison group it did not show an effect by accident, and experimental group showed meaningful effects that self-esteem was increased and anxiety was reduced. Thus, laughter activity can be seen that improving self-esteem, anxiety reduction and etc, is very beneficial to mental health.

• Journal of Biosystems Engineering
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• 제41권4호
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• pp.294-303
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• 2016

Purpose: This study aimed to evaluate the profitability of four selective mechanization systems in rice cultivation. Methods: Field experiments were conducted in the farmers' field during the wet season (June to November) of 2015 in Bangladesh. Mechanization systems were applied to evaluate four different selective levels (treatment) in eleven consequent operations. Seedlings were raised in a traditional seedbed and trays for manual and mechanical transplanting, respectively. Land preparation, irrigation, fertilizer, pesticide, carrying, and threshing and cleaning operations were performed using the same method in all the experimental plots. The mechanical options in the transplanting, weeding, and harvesting operations were changed. The mechanization systems were $S_1$ = hand transplanting + hand weeding + harvesting by sickle, $S_2$ = mechanical transplanting + Bangladesh Rice Research Institute (BRRI) weeder + reaper, $S_3$ = mechanical transplanting + BRRI power weeder + reaper, and $S_4$ = mechanical transplanting + herbicide + reaper. This experiment was performed in a randomized complete block design with four replications. Power tiller, rice transplanter, BRRI weeder, BRRI power weeder, self-propelled reaper, BRRI open drum thresher, and BRRI winnower were used in the respective operations. Accordingly, the techno-economic performances of the different technologies were calculated and compared with those of the traditional system. Results: The mechanically transplanted plot produced 6-10% more yield than the hand transplanted plot because of the use of tender-aged seedlings. Mechanical transplanting reduced 61% labor and 18% cost compared to manual transplanting. The BRRI weeder, BRRI power weeder, and herbicide application reduced 74, 91, and 98% labor, respectively. The latter also saved 72, 63, and 82% cost, respectively, compared to hand weeding. Herbicide application reduced the substantial amount of labor and cost in the weeding operation. Mechanical harvesting also saved 96% labor and 72% cost compared to the traditional method of harvesting using sickle. Selective mechanization saved 15-17% input cost compared to the traditional method of rice cultivation. Conclusions: Mechanical transplanting with the safe use of herbicide and harvesting by reaper is the most cost- and labor-saving operation. The method might be the recommended set of selective mechanization for enhancing productivity.

• 한국철학논집
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• 제41호
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• pp.9-38
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• 2014

한훤당 김굉필(1454~1504)은 고려 말엽 정몽주가 단서를 열어놓은 도학을 하나의 학문 경향으로 승화시켰다. 김굉필은 '조선도학의 시조'로 일컬어졌으며, 이후 4백여 년 동안 도학자의 전형으로 받들어졌다. 김굉필이 씨를 뿌린 도학은 학문과 정치의 기준이 되었다. 조선의 선비들은 김굉필을 본받아 "소학"을 통해 근기(根基)를 배양하였고, 그 기운은 선비의 원기(元氣), 나아가 국가의 원기로 승화하여 국맥(國脈)을 유지하는 원동력이 되었다. 김굉필은 "소학"으로 학문 하는 방법을 제시하였고, "소학"에서 요구하는 강한 실천성을 바탕으로 도덕적 인간, 도덕적 이상사회를 추구하였다. 개인의 수양과 사회 개혁은 서로 다른 차원의 것이 아니다. 김굉필이 몸으로 보인 '자기 수양(律己)'의 정신은 조광조(趙光祖)의 단계에 이르러 도학적 이상국가의 추구로 발전하였다. 김굉필은 '경(敬)'을 통해 "소학"의 가르침을 달성할 수 있다고 보았다. '경'이라는 수양 방법을 통해 마음을 다스리는 것은 조선 성리학의 요체다. 김굉필은 조선의 유학을 '심학(心學)'으로 자리매김하였다. 김굉필 이전에는 심학의 모습을 제대로 선보인 학자가 없었다. 명종 선조 시기 이후로 조선의 유학은 심학으로 정초되어 갔다. 17세기 중엽 이후에는 '경'을 중심으로 한 심학 체계가 공고하게 구축되었다. 김굉필의 문인과 그들이 양성한 학인들은 16세기 중반 이후, 조선의 학계 정계를 이끌었다. 이들은 선조 즉위 이후 사림파(士林派) 집권의 주인공들이었다. 조선을 도덕적 이상국가로 승화시키고 '사림의 나라'로 만든 그 기초는 사실상 김굉필이 놓았다고 할 수 있다.

• 복식
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• 제46권
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• pp.17-30
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• 1999

The purpose of this study is to investigate the philosophy behind the Kasaya and its evolution through its clothing material colour structure and sewing. According to the study the results were as follows. 1. The philosophy behind the dressing of the Kasaya can be found in the Therefore wearing the Kasaya symbolizes the Buddhist philosophy of Jat'ailshisongbul ("Simultaneous attaining of Buddhahood for Everyone") 2. The material symbolizes endurance and contentiment by being satisfied with any kind of clothes may they be good or bad. The material of the kasaya comes to signify the harmony among the monk's community 3. The Kasaya uses peculiar colours and the prohibits the use of the five primary and seconary colous. This is to symbolize equality wherein there is no distinction between the noble and the mean. 4. The structure of the Kasaya according the takes the form of a field The Jo is cut lengthwise and divided in nine grades following the Mahayana tradition as written in the This idea reflects the philosophy of salvation of the Pure land thought. The Je has many Jang('long') and fex Dan('short') The meaning of this is that all living beings by self-cultivation can overcome the dirty law of the secular world and follow the way of the sage who have attained their Buddha nature. 5. With respect to the sewing after finishin the sewing the Jang and the Dan are combined together representing the interdependence between the holy person and the common person. Therefore the sewing ssymbolizes the interconnection among all living beings as it arise from the "conditional causation" law. As I have shown above the philosophy behind of the Kasaya developed and was enriched as it cross over China from India to the Original Buddhist thought with which was created in India was added the richness of the Mahayana buddhist thought. As I have shown above the philosophy behind of the Kasaya developed and was enriched as it cross over China from India to the Original Buddhist thought with which was created in India was added the richness of the Nahayana Buddhist thought with which was created in India was added the richness of the Mahayana buddhist thought. As a result the Dasaya came to signify the "field image". The field-image symbolized by the kasaya came to signify the "heart-field" of all living beings. The "heart-field" by its cultivation is field with blessings. Therefore from the sanghati the Nine Grade the evolution and changes in the designs of the kasaya through the different periods of history became clearly an expression how it is possible for anybody to attend the Buddha nature. This changes also show how the Buddhist precepts became reformed from the original Buddhism.ecepts became reformed from the original Buddhism.

• 농업과학연구
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• 제39권4호
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• pp.497-502
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• 2012

Since the year 2000, the production and consumption of broccoli have rapidly increased in Korea. And, the average production area and amount were about 1,700 ha and 29,000 ton for the past 5 years. Even with the increase of these cultivation and consumption, more than 95% of the broccoli seeds are currently imported from foreign countries such as Japan and Netherlands. Therefore, development of domestic broccoli varieties is needed to relieve Korean farmers' production cost for broccoli. In this situation, National Institute of Horticultural and Herbal Science (NIHHS) of Korea has tried to develop F1 hybrid varieties from elite lines that were obtained by microspore cultivation method from 2008. As the results, about 850 lines of broccoli were obtained and self-pollinated. Then their ploidy levels of the genome were confirmed to select double haploid (D.H.) lines. And the D.H. lines' horticultural traits were evaluated in open field. After the selection of 17 elite D.H. lines, they were cross-pollinated with a male sterile (MS) line to produce F1 hybrid seeds. After 2 to 3 years field trials of these F1 hybrid varieties at the area of Suwon, Gangneung, and Jeju respectively, two hybrid varieties such as 'Wongyo8011' and 'Wongyo8012' are selected for the application of variety protection. With these 4 years of research, we found that the microspore cultivation method is a powerful tool for the conventional breeding program, especially for the development of various inbred lines and even F1 hybrid varieties in short time.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.108-108
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• 2017

The objective of this study was to know prospect and present state of new variety breeding of Forage and Grassland in South Korea. The industry of forage in Korea is going up to expend through utilization of good quality forage, Italian ryegrass, forage corn and oat rather than rice straw. Especially, since 2007, Italian ryegrass(IRG) had been very important major winter forage crop in South Korea and developed 13 varieties including very early maturity variety (three varieties), early maturity variety (three varieties), medium maturity variety (one variety) and late maturity variety (six varieties). But the disadvantage of Italian ryegrass was weak winter hardness and drought but has good advantage of forage nutritive value, high-yielding and high sugar content, and like livestock as like hanwoo, dairy cattle, goat so on. The Ko-variety (Korea developed variety) of Italian ryegrass has high cold-tolerant and adaptability more than any other country developed variety, and expend to cultivation area from southern area (below Daejeon) to middle-northern area (upper Han river). Although the cultivation area of Italian ryegrass of South Korea was 21,700 ha in 2007, right now, that of Italian ryegrass is about 123,600ha due to expend cultivation area and know famer to good forage crop and have a various maturity varieties (very early, early, medium, late) according to local situation (before-crop harvesting stage or double cropping system). The seed market of Italian ryegrass in South Korea becoming extended to around 100 million Korean won and seed export get nearer to foreign country. We are going to develop of new variety for stress tolerant and high yield and quality forage variety, good adaptability to the Korean environmental conditions including reclaimed area, make self-sufficiency system for forage seed (Italian ryegrass), export our seed to foreign countries.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.681-688
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• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• Mycobiology
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• 제43권1호
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• pp.24-30
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• 2015

The effects of monokaryotic strains on fruiting body formation of Lentinula edodes were examined through mating and cultivation of the mated dikaryotic mycelia in sawdust medium. To accomplish this, monokaryotic strains of L. edodes were isolated from basidiospores of the commercial dikaryotic strains, Chamaram (Cham) and Sanjo701 (SJ701). A total of 703 matings (538 self-matings and 165 outcrosses) were performed, which generated 133 self-mates and 84 outcross mates. The mating rate was 25% and 50% for self-mating and outcross, respectively. The bipolarity of the outcross indicated the multi-allelic nature of the mating type genes. The mating was only dependent on the A mating type locus, while the B locus showed no effect, implying that the B locus is multi-allelic. Next, 145 selected dikaryotic mates were cultivated in sawdust medium. The self-mated dikaryotic progenies showed 51.3% and 69.5% fruiting rates for Cham and SJ701, respectively, while the fruiting rate of the outcross mates was 63.2%. The dikaryotic mates generated by mating with one of the monokaryotic strains, including A20, B2, E1, and E3, showed good fruiting performance and tended to yield high fruiting body production, while many of the monokaryotic strains failed to form fruiting bodies. Overall, these findings suggest that certain monokaryotic strains have traits enabling better mating and fruiting.

• 대순사상과 동아시아종교
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• 제2권2호
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• pp.33-50
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• 2023

'Fasting of the Mind (心齋, ch. xīn zhāi)' is arguably the most important concept within the practical approach to the spiritual cultivation formulated by the Daoist philosopher, Zhuangzi (莊子). Most scholars have interpreted 'Fasting of the Mind' as an apophatic practice centered around the aim of the 'Dissolution of the Self (喪我, ch. sàng wŎ).' The Korean new religious movement, Daesoon Jinrihoe (大巡眞理會), can be shown to instead consistently utilize cataphatic descriptions of spiritual cultivation based on the 'quieting of the mind (安心, kr. anshim)' and 'quieting of the body (安身, kr. anshin)' with the highest attainable state referred to as the 'Perfected State of Unification with the Dao (道通眞境, kr. Dotong-jingyeong).' While the language used by Zhuangzi and Daesoon Jinrihoe appears quite different on a superficial level, a deeper examination shows that these rhetorical framings are likely negativistic and positivistic descriptions of the same, or at least reasonably similar, phenomena. Zhuangzi, who focused primarily on the body, mind, and internal energy, cautioned practitioners that 'mere listening stops with the ears (聽止於耳, ch. tīng zhǐ yú ěr)' and 'mere recognition stops with the mind (心止於符, ch. xīn zhǐ yú fú).' He therefore encouraged cultivators of the Dao to 'listen with the spirit (聽之以氣 ch. tīng zhī yǐ qì).' The main scripture of Daesoon Jinrihoe states that "The mind is a pivot, gate, and gateway for gods; They, who turn the pivot, open, and close the gate, and go back and forth through the gateway, can be either good or evil (心也者, 鬼神之樞機也, 門戶也,道路也)," and the Supreme God of the Ninth Heaven (九天上帝, kr. Gucheon Sangje) even promises to visit anyone who possesses a 'singularly-focused mind (一心, kr. il-shim).' In both these approaches, there is a sense of what must be kept out of the mind (e.g., external disturbances, strong emotions, malevolent entities) and what the mind should connect with to attain spiritual progress (e.g., spirit, singular focus, the Supreme God). The observations above serve as the main basis for a comparison between the apophatic descriptions of cultivation found in Zhuangzi and their cataphatic counterparts in Daesoon Thought. However, the culmination of this nuanced comparative exploration reveals that while the leanings of Zhuangzi and Daesoon Thought generally hold true, ultimately, both systems of cultivation transcend the categories of apophatic and cataphatic.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.440-446
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• 2004

Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.