• Biomedical Science Letters
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• v.2 no.2
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• pp.199-209
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• 1996

A new test strip to detect leukocytes using the myeloperoxidase in urine was developed. The reagent strip contains tetramethylbenzine, glucose and glucose oxidase. The detection limit was between 10 cells per 1$\mu$l urine(5 cells/hpf), showing greenish yellow color in the range of 10-25 cells/$\mu$l, green color in the range of 75-250 cells/$\mu$l, greenish blue color in the range of 500 cells/$\mu$l. The result can be obtained within two minute. The performance of the new method was evaluated by comparing the results of microscopic examination and other commercial products. Good correlations were shown between the values obtained by our urine strip and those by other commercial products with 172 urine samples. The results were proven that new methods were useful as primary screening reagents to detect leukocytes in urine.

• Analytical Science and Technology
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• v.30 no.6
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• pp.303-311
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• 2017

A new method for obtaining the photoluminescence of footwear impression by using 1,2-indandione (1,2-IND) solution, which is a latent fingerprint-developing reagent, was studied. A binary complex of DL-alanine and 1,2-IND was prepared by spraying a DL-alanine solution and the 1,2-IND solution (an amino acid sensitive reagent) onto dry or wet origin footwear impression deposited on the surface of printed A4 paper. This binary complex reacts with the trace metal component in the footwear impression to form a ternary complex that exhibits photoluminescence. However, when 5-methylthioninhydrin (5-MTN) solution was used instead of 1,2-IND, no consistent photoluminescence was observed even under identical treatment conditions. In addition, when footwear impressions treated with DL-alanine and 1,2-IND solutions were stored under various temperature conditions (30, 40 and $50^{\circ}C$) and various humidity conditions (30 %, 40 %, 50 % and 60 % RH), the contrast between the footwear impression and the background decreased. Optimal footwear impression photoluminescence was obtained when the footwear impressions treated with DL-alanine and 1,2-IND solutions were stored at $30^{\circ}C$ and 30 % RH for 1 h. The sensitivity of the developed method was ccompared with the sensitivities of three known methods - black gelatin lifting, 2,2'-dipyridyl treatment, and 8-hydroxyquinoline treatment. The results showed that the sensitivity of the developed method was worse than that of the black gelatin lifting method but better than that of 2,2'-dipyridyl or 8-hydroxyquinoline treatment method.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.6 no.2
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• pp.75-91
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• 2020

[^99mTc]Tc-Hexamethylpropylene amine oxime (HMPAO) is currently used as a regional cerebral blood flow imaging agent for single photon emission computed tomography (SPECT). The HMPAO ligand exists in two isomeric forms: d,l and meso showing different properties in vivo. Later studies indicated that brain uptake patterns of ^99mTc-complexes formed from separated enantiomers differed. Separation of enantiomers is difficult by fractional crystallizations method. Usually, the substance is obtained in low chemical yield in a time-consuming procedure. Furthermore, the final product still contains some impurity. So we have developed new efficient route for synthesis of R,R- and S,S-HMPAO enantiomeric compounds in 6-steps. Nucleophilic substitution (S_N2) reactions of 2,2-dimethylpropane-1,3-diamine either with S- (1a) or R-methyl2-chloropropanoate (1b) were performed to produce compounds R,R- (2a) or S,S-isomer (2b) derivatives protected with benzylchloroformate (Cbz), respectively. And then Weinreb amide and methylation reaction using Grignard reagent, oxime formation with ketone group and deprotectiion of Cbz group by hydrogenolysis gave S,S- (7a) or R,R-HMPAO (7b), respectively. Entaniomeric compounds were synthesied with high yield and purity without any undesired product. The 7a or 7b kits containing 10 ㎍ SnCl₂-2H₂O were labeled with ^99mTc with high radiolabeling yield (90%).

• Analytical Science and Technology
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• v.26 no.4
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• pp.228-234
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• 2013

Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea. Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very important issue in the area of forensic drug testing. As an effort to develop new screening method, the reactions between three organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy) succinimide (FMOC-NHS) were performed on silica gel ($SiO_2$) TLC plates. Three reference compounds were synthesized and used for the identification, comparison and study of the limit of detection (LOD) of the products obtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generated compounds containing highly UV-active functional groups on the TLC plate after reacting with primary- and secondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL ($2{\mu}L/spot$), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL ($2{\mu}L/spot$). The LODs of the compounds tested were dependent on the concentration of the derivatizing reagent.

• Journal of the Korean Chemical Society
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• v.41 no.2
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• pp.88-97
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• 1997

The silyltriflates$(Ph_{3-n}SiH(OTf)_n)$have been produced by the reaction of triphenylsilane and triflic acid$(CF_3SO_3H)$at low temperature. These highly reactive compounds are a valuable reagent for the synthesis of numerous new functional substituted silane derivatives. The reaction of silyltriflates with alkenyl- and alkynylmagnesium bromide as well as organolithium compounds gave new silanes$Ph_2SiHR(R=\;C(CPh,\;CH=CH_2,\;CH_2CH=CH_2,\; (CH_2)_2CH=CH_2,\;(CH_2)_3CH=CH_2)$in high yields. The hydrosilation of prepared alkenyl- and alkynylsilanesPh_2SiHR$in the presence of a platinum catalyst(Pt/C) at high temperature$(200{\circ}C)$gave carbosilane polymers$((Ph_2SiCH=CPh)_n$and$(Ph_2Si(CH_2)m)n;\;m=2∼4, n{\ge}10)$along with five- and six-membered silaalkane ring compounds derived from intramolecular hydrosilation reactions. All of the prepared compounds are confirmed by NMR, UV, IR and mass spectroscopy as well as elemental analysis.

• IE interfaces
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• v.24 no.2
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• pp.156-163
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• 2011

The radiopharmaceutical synthesis for PET (positron emission tomography) is composed of chemical reactions using automated synthetical equipment. Due to the radioactive material, the automated equipment is being frequently developed to replace human operators who conduct dangerous, repetitive and dexterous operations. As to operation, the manipulating program is commonly coded using the spread sheet while the whole actuators are mapped in every step. The process map (program) is changed according to such parameters as temperature of reactor, keeping time, mixture sequence and amount of reagent. In cases of customizing the automated synthetical equipment or developing the new radiopharmaceuticals, lots of experiments should be conducted and the programming mistake is not allowed as it can lead abnormal control of the equipment to leak the radioactive materials. The exact process map has depended on trial and error manner. Thus, this study developed the methodology to tabulate the synthetical process to convert the process map automatically while the synthetical module formation is represented by a network model. The proposed method is validated using the actual radiopharmaceutical synthetical procedure.

• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.377-382
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• 1985

As an essential previous step towards the development of cell fusion to breed a new brewing yearst strain, several factors predicted to affect the protoplast formation of S. cerevisiae, C. tropicalis and E. fibuligera were investigated in order to obtain the protoplasts in high yields. The optimum pH and temperature for the protoplas formation were 7.5 and 35$^{\circ}C$, respectively. Pretreatment of the yeast cells with 2-mercaptoethanol stimulated the protoplast formation and 50mM of the reagent was found as effective. Among several osmotic stabilizers tested for their effect on protoplas formation, 0.6M KCI was comparatively favorable.

• YAKHAK HOEJI
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• v.46 no.2
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• pp.124-128
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• 2002

The nitrone-based free radical trapping reagent, $\alpha$-phenyl-n-tert-butyl nitrone (PBN) has been proposed as therapeutic agent for stroke. We used this for model drug of development of new drug for neuroprotection. The purpose of this study was to evaluate the blood-brain barrier (BBB) permeability of PBN in Sprague-Dawly (SD) rats. The BBB transport of PBN was investigated in SD rats using internal carotid artery perfusion (ICAP) method at a rate of 4 mι/min for 15 second. We also obtained pharmacokinetic parameters of PBN using single intravenous injection technique. When we estimated BBB permeability of PBN with ICAP method, the brain volume of distribution of PBN was 60.0 $\pm$ 12.0 $\mu\textrm{g}$/ι. The brain uptake of PBN after IV injection at 120 min was 0.15 $\pm$ 0.01%ID/g. The PBN was transported to the brain through the BBB well in rats, because PBN is small molecule (MW 177) and lipid-soluble (log P 1.23) compound.

• Journal of the Korean Society of Food Science and Nutrition
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• v.4 no.1
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• pp.97-100
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• 1975

Degree of polished rice so called 70 percentage of polished rice sold at the 19 places of rice dealer's store in Gwang - Ju city were surveyed through the New M.G.color reaction reagent, from 24th to 30th of January, 1975. The results were as follows ; 1. Nearly the 70 percentage polished rice sold at the rice dealer's store showed some differences in the degree of polishing of rice, with respect to color reaction in comparison with standard 70 percentage polished rice. 2. There have been no rice which are over excessive 70 percentage polished rice. 3. The place were more than 90 percentage of 70percentage polished rice amount, were 3 places in the 19 places rice dealer's store and 50 percentage of below 70 percentage polished rice amount were 8 places in the 19 places.

• Korean Journal of Clinical Pharmacy
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• v.22 no.2
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• pp.160-166
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• 2012

Purpose: The purpose of the present study was to investigate the pharmacokinetics of urea, a new potential diagnosis reagent of Helicobacter pylori infection. Methods: Considering the mechanism of urea breath test, we determined the excretion of urea in expired air after its oral administration in rats and beagle dogs at the dose of 2 mg/kg (including 50 mCi/mmol $^{14}C$-urea 50 ${\mu}Ci/kg$ for rats and 13.5 ${\mu}Ci/kg$ for dogs). Results: Urea was rapidly disappeared from the blood circulation by 1 hr after its i.v. bolus injection, followed by a slow disappearance by 24 hr. The half-lives at the distributive phase ($t_{1/2{\alpha}}$) and post-distributive phase ($t_{1/2{\beta}}$) were 2 min and 6 hr, respectively. The bioavailability of urea was 64.3% after its oral administration. The values of the volume of distribution ($V_{dss}$) and the total body clearance ($CL_t$) after the oral administration were compatible with those after i.v. administration. The recovery of urea in the bile was about 0.1% of the dose by 24 hr after its oral administration. Urea was extensively eliminated in the urine by 48 hr. The recovery ratios of urea in the urine and expired air were about 86.8% and 2.99% of the dose by 48 hr, respectively. Moreover, urea was mostly distributed from the blood circulation to the kidney, followed by being eliminated in the urine without metabolism. The concentration of urea in the kidney was 4.0 times higher than that of plasma at 40 min after its oral administration. Conclusions: These findings indicated that oral route appears to be available for the administration of urea. Orally administered urea, thus, was considered to be useful for the diagnosis of Helicobacter pylori infection.