• 한국식품영양학회지
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• 제22권3호
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• pp.416-420
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• 2009

A formula diet based on pork meat oligopeptides(pork meat protein hydrolysates) was designed for experimental hepatitic rats. The rats were given D-galactosamine for 6 days. During this period, the rats were provided with a 12% casein diet or the formula diet which was low in aromatic amino acids and rich in branched chain amino acids. The formula diet was prepared using pork meat oligopeptides as the principal nitrogen source. The hepatitic rats given the formula diet had lower plasma GOT and GPT concentrations. Additionally, the fischer ratio of the plasma was significantly lower in those rats. However, there was no significant difference in the plasma insulin-like growth factor-I concentration before and after acid-ethanol extraction among groups. These results suggest that the formula diet was better for the animals than the casein diet. Furthermore, these findings suggest that pork meat oligopeptides are an excellent material for preparation of formula diets for patients suffering from hepatitis.

• Journal of Nutrition and Health
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• 제29권6호
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• pp.569-577
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• 1996

This study was performed to determine the effect of maternal protein intake on 1) the concentration of immune substances in milk 2) degree of passive immunity to pups via lactation, and 3) specific antibody production to a specific antigen, $\beta$-lactoglobulin(BLG). 4) the effect of passive immunity that pups received from mother during lactation on the production of antibodies when the pups were challenged to the same antigen. Part of the female rats were immunized with BLG before and during pregnancy. The pregnant rats were placed into either 25% or 10% isolated soy protein diet throughout gestation and lactation. After weaning, pups from each group continued to be fed the same diet. At 18 weeks of age, all the pups were challenged with BLG. Total IgA and IgG, lysozyme, BLG-specific IgA and IgG were measured in dam's serum, dam's milk, and pup's serum. Total IgG, and lysozyme in dam's serum and milk were higher in high protein group. Total IgA and IgG in pup's serum remained higher in high protein group from 5 to 18 weeks of age. BLG-specific antibodies were found in the milk and serum of immunized dams, and in serum of pups born to immunized dams but not in the non-immunized group. BLG-specific IgA and IgG were again higher in high protein group and declined with time. The concentration decreased faster in the low proetein group than in the high protein groups. After immunization the pups with LBG, serum BLG-specific antibodies were not differ between rats born to immunized dams and those born to non-immunized dams. Therefore passive immunity rats received via milk as a pup had no effect on the BLG-specific antibody production later in life. This study shows the importance of protein status of mother and strongly support to the endorsement of breast feeding.

• Asian-Australasian Journal of Animal Sciences
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• 제10권3호
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• pp.274-276
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• 1997

The effects of cecectomy on nitrogen utilization and nitrogen excretion were examined in single comb white leghorn (SCWL) cockerels fed a 5% protein diet supplied with urea. The cecectomy tended to increase nitrogen balance and nitrogen utilization and significantly decreased uric acid excretion (p < 0.01). Urea and ammonia excretion tended to be about 60% increased and decreased by cecectomy in SCWL cockerels, respectively, but blood ammonia, urea and uric acid concentrations were not affected. The results are in good agreement with those obtained previously in cecum-ligated chickens. It is concluded that the improvement of nitrogen utilization and decreases in urinary uric acid excretion in cecectomized chickens do not result from the modification of cecal fermentation.

• 한국식품과학회지
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• 제8권4호
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• pp.263-272
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• 1976

According to the intercombined review of chemical and biological investigation it has been noted that the metabolizable energy per gram dietary protein of mixed diet of daily intake patterned by Korean population has been found 3.4-3.6 Cal., which entails 10-12% level of the protein calorie percentage of total metabolizable energy, the biological value being fallen within the scope 63-73. The structure of dietary protein has revealed that the lysine and isoleucine were primary limiting amino acids and threonine secondary limiting as a general trend, however, it is assumed that the ultimate nutritional effect of dietary protein might be restricted uniformly among regions by the amount of lysine, since the lysine availability has been yielded as low as 72-82% level. As for the net protein utillization NPUst falls in the range of 52-62 and the NPUop 47-58. In either part the mountainous region has demonstrated lowest value and the urban area highest, these trend being obviously associated with the ratio of animal protein relative to the vegetable origin. The net dietary protein calorie percentage (NDpCal %) has been found within the range of 5-7 that may be capable of meeting the requirement for the maintenance of adult, though for the growth it is insufficient. Present level of total caloric intake would not influence on the fate of protein value of prevailing regional diet in terms of caloric restriction, since the present intake of food energy is higher than the lower limit of caloric intake that would impair the biological performance of dietary protein fed ad libitum basis. Based on the protein efficiency, the adequacy of current level of protein intake was analyzed in terms of utilizable protein, and it has been demonstrated that the 37.8g of utilizable protein in the fishery region and 38.2g in the mountainous region were bellow the FAO recommendation. Accordin to the hematological study it may be interpreted that the anemic symptoms of the mountainous region has some possibility of being related to the inferior status of dietary protein in quality as well as in quantity.

• 대한가정학회지
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• 제36권12호
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• pp.201-216
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• 1998

This study was conducted to assess the nutrient intake and diet quality of college students in Seoul. Dietary survery data were obtained by 24-hour recall method from 774 students, 553 males and 191 females. Mean daily intake of energy was 2,480kcal with 56.4% of energy intake from carbohydrate, 14.6% from protein, and 25.5% from fat. Percentage of fat was higher than mean values of National Nutrition Survey(NNS), adults in rural area or younger students in previous studies. Mean daily intakes of calcium and vitamin A did not meet RDA levels in both sexes and intake of iron in females was insufficient to meet RDA level. The percentage of animal food to total food intake was 25% and consumption of meat, beverage and milk group was higher than those of National Nutrition Survey(NNS). When the consumption of 5 major food groups(grain, meat, milk, fruit, vegetable) were evaluated, 22% of students consumed all 5 groups and 47% consumed 4 groups, which show more balanced food group intake than adults in rural area. Food groups which were omitted frequently were milk and fruit. These results show that college students in Seoul consume better diet than subjects of National Nutrition Survey(NNS) or adults in rural area. However, high fat intake, low calcium and vitamin A intake and low iron level in women were observed. Majority(78%) of students had at least one food group omitted in their diet. Appropriate nutrition education may help to improve the quality of diet in these students.

• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1513-1520
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• 2009

Forty-six Holstein heifers were used in a completely randomized design and assigned to 1 of 2 treatments to evaluate the effects of 2 diets varying in ruminal fermentable carbohydrate sources, namely ground corn (GC) and rolled wheat (RW), on metabolism and performance of primiparous cows in the periparturient period. The heifers were fed diets as a total mixed ration (TMR) with similar energy and crude protein content including i) 18.57% GC, or ii) 18.57% RW from -24.13${\pm}$7.73 d relative to expected calving until calving. After calving, all animals received the same lactation diet until 28 d. Animals were group fed from the beginning of the study to -7 d relative to expected calving, fed individually from d -7 to 7 days in milk (DIM), and again group fed to 28 DIM. The pre-partum diets affected (p<0.05) dry matter intake (DMI), energy intake, energy balance (EB) and urinary pH during the last week pre-partum. There was no effect of pre-partum carbohydrate source on overall plasma concentration of glucose, nonesterified fatty acid (NEFA), $\beta$-hydroxybutyrate (BHBA), albumin, triglyceride (TG), cholesterol, aspartate aminotransferase (AST), insulin, and cortisol during the periparturient period. Cows fed the RW diet during the pre-partum period had greater calcium for the first week (p<0.05) and during 28 d (p = 0.08) of lactation compared with heifers fed the GC diet. Primiparous cows fed the RW diet produced greater milk protein content and yield (p<0.05). Primiparous cows fed the RW diet had lower milk urea nitrogen (MUN) and somatic cell count (SCC) than cows fed the GC diet (p<0.05). The results of this study show that feeding pre-partum diets with a rapidly fermentable source of starch but low energy content can improve animal metabolism and performance and smooth the transition of primiparous Holstein cows from gestation to lactation.

• Nutrition Research and Practice
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• 제13권6호
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• pp.529-534
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• 2019

BACKGROUND/OBJECTIVES: The leaves of Moringa oleifera (MO) and Moringa stenopetala (MS) commonly grown in Ethiopia possess potential nutritional and medicinal value. The aim of this study was to evaluate the nutritional and functional characteristics of the dried leaf powder from two Moringa species to develop sustainable nutritional supplements for Ethiopians from locally grown plant sources. MATERIALS/METHODS: Freshly harvested and air-dried MO and MS leaves were authenticated and the nutritional contents, such as protein, ash, lipids, and selected vitamins and minerals, were analyzed using standard analytical procedures. Amino acid compositions were also determined by an amino acid analyzer. Nine-week-old mice were randomly divided into four groups to investigate the anti-obesity effects of Moringa. The first group was fed a basal diet, the second group a high-fat diet, and the others were fed a high-fat diet containing 0.1% Moringa leaf powder from each species. After seven weeks, serum indices related to lipid profiles from each mouse were analyzed. RESULTS: The present study revealed high protein (28-29%) and ash (7-11%) contents. Glutamic acid, aspartic acid, proline, and leucine were the most abundantly found amino acids in both species. The predominant minerals in the leaf powder were calcium (826-1,530 mg/100 g), potassium (794-904 mg/100 g), and magnesium (286-431 mg/100 g). Pyridoxine (475.06 mg/100 g) and vitamin E (34.2 mg/100 g) were found only in MS. Niacin was found only in MO at 32.21 mg/100 g, whereas ascorbic acid was found in both species (3.89 and 6.19 mg/100 g dry weight for MO and MS, respectively). The results of the animal study showed that mice on a high-fat diet containing 0.1% MO leaf powder alleviated the elevation of cholesterol, triglycerides, and low-density lipoprotein cholesterol induced by the high fat diet. MO was more effective than MS in preventing hypercholesterolemia and fat deposition. CONCLUSION: The findings in this work confirmed that Moringa leaves of both MO and MS possessed high nutritional value but MO was better at preventing the harmful effects of the high-fat diet than MS.

• Journal of Nutrition and Health
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• 제12권3호
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• pp.9-23
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• 1979

The known risk factors for atherosclerosis include plasma low density lipoproteins (LDL)or Cholesterol, low PUFA in the diet, hypertension, and high Na intake, obesity, diabetes, lack of exercise, cigarette smoking, sugar, low fiber and nicotinic acid in the diet, sources of Protein foods, and Psychological factors. Among various dietary factors, fat is known as the most serious causative agent for atherosclerosis. The genetic factor is a18o known as an important one but is out of scope in this paper. Since atherosclerosis is a progressive disease which may develop for many years before showing any definitive symptoms, it is very important to develop preventive programs especially in the country like Korea that is not quite overdevelopted as some western countries. In this paper all the factors mentioned above were reviewed and the dietary suggestion were made on the basis of the content of polyunsaturated and saturated fat in the diet to prevent or/and to cure this disease. Most of the available data on diet therapy with emphasis. on P/S ratio were tabulated together. after tile patient's habitual dietary intake is analized the guidelines of personalized fat-controlled diet can be recommended. It is of utmost importance to develop Korean diets for beth prevention and cure of atherosclerosis emphasizing individual eating habit in the near future which can be more Practicaly used both at home and in the hospital.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.330-342
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• 2022

We aimed to investigate candidate proteins related to long-term caloric restriction and feed efficiency in bovine longissimus dorsi muscle (LM). A total of 31 Korean native steers were randomly distributed to ad libitum (n = 16) or caloric restriction group (n = 15) to conduct two feeding trials for 13 mon. In the first trial (10-18 mon of age), steers were fed with 100% ad libitum (NE_g = 0.63 Mcal/kg) or caloric restriction (80% of the previous day's feed intake of ad libitum group). In the second trial (18-23 mon of age), the energy value of 100% ad libitum diet was 1.13 Mcal/kg NE_g and those in caloric restriction group diet was 0.72 Mcal/kg NE_g. At the endpoint of this experiment, in each group, 6 animals were selected with high (n = 3) or low feed efficiency (n = 3) to collect muscle tissue samples (6 animals/group). From muscle tissues of 23 mo of age, we excavated 9 and 12 differentially expressed (two-fold or more) proteins in a nutritional group and feed efficiency group using two-dimensional electrophoresis, respectively. Of these proteins, heat shock protein beta-6 was up-regulated in both the caloric restriction and the low feed efficiency group. In bovine embryonic fibroblasts, the mRNA expression of heat shock protein beta-6 increased after adipogenic differentiation, however, decreased after myogenic differentiation. Our data provide that heat shock protein beta-6 may be an adipogenic protein involved in the mechanism of caloric restriction and feed efficiency in the LM of the steer.

• 한국가금학회지
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• 제29권3호
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• pp.195-204
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• 2002

산란계에 대한 오전사료와 오후사료의 영양수준 및 급여방법이 산란능력과 경제성에 미치는 영향을 구명하기 위해 2개의 실험을 실시하였는데, 실험 1에서는 ISA Broun 갈색산란계 384수를 4처리 3반복, 반복당 32수씩을 공시하여 28~38주령에 2주간의 예비사양기간을 거쳐 본 시험 8주간 실시하였다. 대조구(C)는 관행적인 산란계 단일 배합사료를 급여하였고, 시험구들(T1, T2 및 T3)은 고에너지-고단백질-저 Ca의 오전사료와 저에너지-저단백질-고Ca의 오후사료를 별도로 급여하였다. 실험 2는 “ISA Brown” 갈색 산란계 384수를 3처리 4반복, 반복당 32수씩을 공시하여 48 ~ 58주령에 2주간의 예비사양기간을 거쳐 본 시험 8주간 실시하였다. 대조구(C)는 관행적인 산란계 단일 배합사료를 급여하였고, T1은 고에너지-고단백질-저Ca의 오전사료와 저에너지-저단백질-고Ca의 오후사료를 별도로 급여하였고, T2는 오전사료를 분말로 하고 오후사료를 펠렛으로 가공하여 50：50으로 혼합급여 하였다. 실험 1에서 헨데이 산란율과 평균난중 및 1일 1수당사료 섭취량은 대조구와 시험구들(T1-T3)간에 유의적인 차이가 없었다. 1일 1수당 ME, CP 섭취량 및 사료비는 대조구에 비해 시험구들(T1-T3)이 유의적으로 적었고(P<0.05), 시험구들간에는 오후사료의 ME및 CP 수준이 감소하고 Ca수준이 높아질수록 감소하는 경향이 있었다. 산란 개당 및 산란 kg당 사료, ME CP요구량과 사료비도 모두 대조구에 비해 시험구(T1-T3)들이 유의적으로 적었으며(P<0.05), 시험구들 간에는 오후사료의 Ca함량이 대조구에 비해 4배가 많은 73처리가 사료요구량에서 71과 72보다 많았고(P<0.05), 오후사료의 ME와 CP수준이 감소하고 Ca수준이 증가할수록 감소하는 경향을 보였다. 계란의 비중과 난각강도 및 난각후도 등 난각질은 모두 대조구에 비해 시험구(71-73)들이 유의적으로 향상되었고(P<0.05),오후사료의 Ca수준이 증가할수록 향상되는 경향을 보였다. 실험 2에서 헨데이 산란율은 처리간에 유의적인 차이가 없었으나, 평균난중과 1일 1수당 사료섭취량은 대조구에 비해 오전사료와 오후사료의 별도급여구(T1)와 혼합급여구(T2)가 감소하는 경향을 보였다(P<0.05). 1일 1수당 ME, CP섭취량 및 사료비는 모두 대조구에 비해 T1과 T2구가 유의적으로 감소하였고(P<0.05), T1과 T2간에는 유의차가 없었다. 산란 개당 ME와 CP요구량 및 사료비는 모두 대조구에 비해 T1과 T2구가 유의적으로 감소하였으나(P<0.05), 산란kg 당 사료, ME, CP요구량 및 사료비는 모두 처리간에 유의적인 차이가 없었다. 계란의 비중과 난각후도는 대조구에 비해 T1과 T2구가 유의적으로 향상되었고(P<0.05), 난각강도는 처리간에 유의적인 차이가 없었으나, T1과 T2구가 대조구보다 향상되는 경향을 보였다. 본 시험 결과 오전용 사료는 관행적인 산란계 배합사료에서 Ca공급제를 제외한 것을 급여하고, 오후용 사료는 Ca공급제를 3배 첨가한 T2처리로 15:00~16:00시에 교체급여를 하면 사료섭취량 감소와 사료비 절감면에서 바람직할 것으로 사료되며, 고에너지-고단백질-저Ca의 분말사료와 저에너지-저단백질-고Ca의 펠렛사료를 혼합급여하면 산란계의 사료섭취 량과 사료비를 절감할 수 있었으며, 오전사료와 오후사료 별도급여의 번거로움을 피할 수 있는 사양체계로 기대가 되나 보다 더 많은 연구가 필요하다.