• Title/Summary/Keyword: A key technique

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Endophytic fungi harbored in Panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease

  • Zheng, You-Kun;Miao, Cui-Ping;Chen, Hua-Hong;Huang, Fang-Fang;Xia, Yu-Mei;Chen, You-Wei;Zhao, Li-Xing
    • Journal of Ginseng Research
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    • v.41 no.3
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    • pp.353-360
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    • 2017
  • Background: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy Panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of P. notoginseng. Methods: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of P. notoginseng. Results: A total of 89 fungi were obtained from the roots, stems, leaves, and seeds of P. notoginseng, and 41 isolates representing different morphotypes were selected for taxonomic characterization. The fungal isolates belonged to Ascomycota (96.6%) and Zygomycota (3.4%). All isolates were classified to 23 genera and an unknown taxon belonging to Sordariomycetes. The number of isolates obtained from different tissues ranged from 12 to 42 for leaves and roots, respectively. The selected endophytic fungal isolates were challenged by the root-rot pathogens Alternaria panax, Fusarium oxysporum, Fusarium solani, Phoma herbarum, and Mycocentrospora acerina. Twenty-six of the 41 isolates (63.4%) exhibited activity against at least one of the pathogens tested. Conclusion: Our results suggested that P. notoginseng harbors diversified endophytic fungi that would provide a basis for the identification of new bioactive compounds, and for effective biocontrol of notoginseng root rot.

An Enhanced Secure Health Data Transmission Protocol using Key Insulation in Remote Healthcare Monitoring System (원격 헬스케어 모니터링 시스템에서 키 격리기법을 이용한 개선된 건강정보 전송 보안 프로토콜)

  • Noh, Si-Wan;Park, Youngho;Rhee, Kyung-Hyune
    • Journal of Korea Multimedia Society
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    • v.19 no.12
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    • pp.1981-1991
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    • 2016
  • In recent, the advancement of wearable devices and wireless body area networking technologies motivate researchers to pay attention to remote healthcare system for monitoring patients health and disease progression effectively. However, in order to implement a practical remote healthcare system, we must consider the security and privacy of patient's personal health information transmitted to healthcare servers through the network. Hence, in this paper, we propose a secure health data transmission protocol in remote healthcare monitoring system to protect patient's health information and prevent privacy from eavesdropping on the network. To achieve our security goals, we design an efficient secure protocol based on the identity-based cryptography with key evolution technique, and then confirm the superiority and the efficiency of the proposed protocol as compared with the existing protocol of Yang et al.

Rational finite element method for plane orthotropic elastic problems

  • Mao, Ling;Yao, Weian;Gao, Qiang;Zhong, Wanxie
    • Structural Engineering and Mechanics
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    • v.51 no.6
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    • pp.923-937
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    • 2014
  • The rational finite element method is different from the standard finite element method, which is constructed using basic solutions of the governing differential equations as interpolation functions in the elements. Therefore, it is superior to the isoparametric approach because of its obvious physical meaning and accuracy; it has successfully been applied to the isotropic elasticity problem. In this paper, the formulation of rational finite elements for plane orthotropic elasticity problems is deduced. This method is formulated directly in the physical domain with full consideration of the requirements of the patch test. Based on the number of element nodes and the interpolation functions, different approaches are applied with complete polynomial interpolation functions. Then, two special stiffness matrixes of elements with four and five nodes are deduced as a representative application. In addition, some typical numerical examples are considered to evaluate the performance of the elements. The numerical results demonstrate that the present method has a high level of accuracy and is an effective technique for solving plane orthotropic elasticity problems.

An Analysis on Key Factors of Mobile Fitness Application by Using Text Mining Techniques : User Experience Perspective (텍스트마이닝 기법을 이용한 모바일 피트니스 애플리케이션 주요 요인 분석 : 사용자 경험 관점)

  • Lee, So-Hyun;Kim, Jinsol;Yoon, Sang-Hyeak;Kim, Hee-Woong
    • Journal of Information Technology Services
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    • v.19 no.3
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    • pp.117-137
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    • 2020
  • The development of information technology leads to changes in various industries. In particular, the health care industry is more influenced so that it is focused on. With the widening of the health care market, the market of smart device based personal health care also draws attention. Since a variety of fitness applications for smartphone based exercise were introduced, more interest has been in the health care industry. But although an amount of use of mobile fitness applications increase, it fails to lead to a sustained use. It is necessary to find and understand what matters for mobile fitness application users. Therefore, this study analyze the reviews of mobile fitness application users, to draw key factors, and thereby to propose detailed strategies for promoting mobile fitness applications. We utilize text mining techniques - LDA topic modeling, term frequency analysis, and keyword extraction - to draw and analyze the issues related to mobile fitness applications. In particular, the key factors drawn by text mining techniques are explained through the concept of user experience. This study is academically meaningful in the point that the key factors of mobile fitness applications are drawn by the user experience based text mining techniques, and practically this study proposes detailed strategies for promoting mobile fitness applications in the health care area.

A Design for Network Security System via Non-security Common Network (일반망과 보안망을 연계한 네트워크 보안체계 설계)

  • Cho, Chang-Bong;Lee, Sang-Guk;Dho, Kyeong-Cheol
    • Journal of the Korea Institute of Military Science and Technology
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    • v.12 no.5
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    • pp.609-614
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    • 2009
  • In this paper, we have proposed a design for security network system passing through the non-security network which is commonly used for various networking services. Based on the security requirements which are assumed that the large classified data are bi-transmitted between a server and several terminals remotely located, some application methods of security techniques are suggested such as the network separation technique, the scale-down application technique of certification management system based on the PKI(Public Key Infrastructure), the double encryption application using the crypto-equipment and the asymmetric keys encryption algorithm, unrecoverable data deleting technique and system access control using USB device. It is expected that the application of this design technique for the security network causes to increase the efficiency of the existing network facilities and reduce the cost for developing and maintaining of new and traditional network security systems.

A practical subcritical rod worth measurement technique based on the improved neutron source multiplication method

  • Jiahe Bai;Chenghui Wan;Ser Gi Hong;Hongchun Wu
    • Nuclear Engineering and Technology
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    • v.56 no.4
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    • pp.1398-1406
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    • 2024
  • The control rod worth is a key safety parameter required to be measured in commercial pressurized water reactors (PWRs). Conventionally, the control rod worth is measured after reaching the critical state, which occupies the considerable time in the zero-power physics test. In this study, an efficient control-rod worth measurement technique has been proposed based on the improved neutron-source multiplication method, which can be implemented with the source-range detector count rates in the subcritical states. Moreover, the noise reduction technique has been adopted to smooth the large fluctuation existing in the original signals. In order to verify the engineering performance of the proposed measurement technique, the measured source-range detector count rates during the rod withdrawal process before reaching critical state in a CNP1000 reactor have been employed. It demonstrated that almost all estimated results of control rod worth satisfy the engineering acceptance criteria, except one control rod with the relative difference over 10 %, which indicates the capability of the proposed method in estimating control rod worth.

Source Current Control Strategy of Active Power Filters for Unbalanced Load Compensation in Three-Phase Four-Wire Distribution Networks

  • Wang, Lei;Han, Xiaoqing;Meng, Runquan;Ren, Chunguang;Wang, Qi;Zhang, Baifu
    • Journal of Power Electronics
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    • v.18 no.5
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    • pp.1545-1554
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    • 2018
  • This paper proposes a modified control strategy to improve the performance of three-phase four-leg shunt active power filters (APFs) for the compensation of three phase unbalanced loads. Unbalanced current cannot be obtained accurately by a harmonic detector due to the lower frequency. The proposed control strategy eliminates conventional harmonic detectors by directly regulating the source current. Therefore, the computational complexity is greatly reduced and the performance of the APF is improved. A mathematic model has been developed based on the source currents. The corresponding controllers have been designed based on the sinusoidal internal model principle. The proposed control strategy can guarantee excellent compensation performance and stable operation after an extreme disturbance such as a short circuit fault. In addition, the proposed technique can selectively compensate specific harmonics. A 50kVA prototype APF is implemented in the laboratory to validate the feasibility and performance of the proposed control strategy.

A Rapid Preconcentration Method Using Modified GP-MSE for Sensitive Determination of Trace Semivolatile Organic Pollutants in the Gas Phase of Ambient Air

  • He, Miao;Xu, Qingjuan;Yang, Cui;Piao, Xiangfan;Kannan, Narayanan;Li, Donghao
    • Bulletin of the Korean Chemical Society
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    • v.35 no.10
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    • pp.2995-3000
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    • 2014
  • A sensitive concentration method utilising modified gas-purge microsyringe extraction (GP-MSE) was developed. Concentration (reduction in volume) to a microlitre volume was achieved. PAHs were utilised as semivolatile analytes to optimise the various parameters that affect the concentration efficiency. The injection rate and temperature were the key factors that affected the concentration efficiency. An efficient concentration (75.0-96.1%) of PAHs was obtained under the optimised conditions. The method exhibited good reproducibility (RSD values that ranged from 1.5 to 9.0%). The GP-MSE concentration method enhances the volume reduction (concentration factor), leading to a low method detection limit ($0.5-15ngL^{-1}$). Furthermore, this method offers the advantage of small-volume sampling, enabling even the detection of diurnal hourly changes in the concentration of PAHs in ambient air. Utilising this method in combination with GC-MS, the diurnal hourly flux of PAHs from the gas phase of ambient air was measured. Indeed, the proposed technique is a simple, fast, low-cost and environmentally friendly.

Strain demand prediction of buried steel pipeline at strike-slip fault crossings: A surrogate model approach

  • Xie, Junyao;Zhang, Lu;Zheng, Qian;Liu, Xiaoben;Dubljevic, Stevan;Zhang, Hong
    • Earthquakes and Structures
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    • v.20 no.1
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    • pp.109-122
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    • 2021
  • Significant progress in the oil and gas industry advances the application of pipeline into an intelligent era, which poses rigorous requirements on pipeline safety, reliability, and maintainability, especially when crossing seismic zones. In general, strike-slip faults are prone to induce large deformation leading to local buckling and global rupture eventually. To evaluate the performance and safety of pipelines in this situation, numerical simulations are proved to be a relatively accurate and reliable technique based on the built-in physical models and advanced grid technology. However, the computational cost is prohibitive, so one has to wait for a long time to attain a calculation result for complex large-scale pipelines. In this manuscript, an efficient and accurate surrogate model based on machine learning is proposed for strain demand prediction of buried X80 pipelines subjected to strike-slip faults. Specifically, the support vector regression model serves as a surrogate model to learn the high-dimensional nonlinear relationship which maps multiple input variables, including pipe geometries, internal pressures, and strike-slip displacements, to output variables (namely tensile strains and compressive strains). The effectiveness and efficiency of the proposed method are validated by numerical studies considering different effects caused by structural sizes, internal pressure, and strike-slip movements.

Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms

  • Fu, Yeqi;Liu, Yunqiu;Abuzeid, Asmaa M.I.;Huang, Yue;Zhou, Xue;He, Long;Zhao, Qi;Li, Xiu;Liu, Jumei;Ran, Rongkun;Li, Guoqing
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.9-15
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    • 2019
  • Melting temperature shift ($T_m-shift$) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a $T_m-shift$ method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5' end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of $T_m-shift$ was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The $T_m-shift$ method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of $T_m$- values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was $5.22{\times}10^{-6}$ and $5.28{\times}10^{-6}ng/{\mu}l$ samples of AceP and AtuP, respectively. The accuracy of $T_m-shift$ method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the $T_m-shift$ detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.