• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.1466-1467
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• 2008

We describe here a novel micro total analysis system for the purification and identification of the affinity-captured proteins. Also we demonstrated the mass analysis of the Carcinoembrionic antigen (CEA) and Alpha femtoprotein which were chosen as the target cancer marker. For MALDI-TOF analyses, the proteins should to be separated from a protein mixture and be concentrated when needed. This procedure usually takes a long time even before protease-digested samples are to be obtained from them. Here, we describe integrated and efficient micro chip for protein purification and digestion for MALDI-TOF analyses. At first, disease protein is purified by passing the micro chamber from a protein mixture or human whole serum and released from the micro affinity beads by thermal heating. Purified protein is then transfer to the hole for trypsin digestion. The final sample is analyzed by MALDI-TOF. All the processes could be finished successfully within one hour, which renders MALDI-TOF analyses of a target protein quite simple.

• Food Engineering Progress
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• v.22 no.4
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• pp.283-294
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• 2018

Food allergy is a chronic disease that is increasing all over the world, and it can even lead to a loss of life. To prevent any incidents resulting from food allergies, most countries keep strengthening their food allergen labeling requirements domestically and internationally, with a constant monitoring system against undeclared allergens and recall of offending products. In order to avoid economic losses to industry and damages to international relations from undeclared allergens, it is necessary to confirm each country's regulatory policy on food allergen labeling prior to exportation. Another required action is to try for a reduction of the cross-contamination risk of the allergens during manufacturing and storage, which should be verified by using an accurate and reliable analysis of food allergens. This paper is intended to provide an introduction to the regulation of food allergen labeling by country, allergen management methods to avoid cross-contamination, and allergen detection methods using ELISA, PCR, and LC/MS. Changes of allergenicity during thermal or nonthermal processing also will be investigated in our review. This review will be helpful for the food industry to better understand patients suffering from food allergies and to manage food allergens in food manufacturing.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.3
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• pp.448-452
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• 2004

Trauma from occlusion(TFO) is injury to the periodontal tissue as a result of occlusal forces. Mobility is a common clinical sign of occlusal trauma. In acute occlusal trauma, this may be accomanied by pain, tenderness to percussion, thermal sensitivity, and pathologic tooth migration. Chronic occlusal trauma may be marked by excessive wear and gingival recession. Radiographic finding include a widened periodontal ligament space, radiolucence and condensation of the alveolar bone and root resorption. TFO is related to the pathogenesis of periodontal disease. It can cause increased tooth mobility TFO itself does not initate or aggravate marginal gingivitis or initiate periodontal pockets. Active trauma can accelerate bone loss, pocket formation and gingival recession depending on the presence of local irritants and inflammation. Gingival recession associated with occlusal forces includes traumatic crescent, McCall's festoon and Stillman's cleft. TFO plays a minor role in the pathogenesis of early to moderate periodontitis. A 5-year-old male visited Yonsei University Pedodontics clinic with a chief complaint about gingival recession. Mobility, excessive wear, gingival recession were detected by clinical exam on the both mandibular deciduous ca nine. On the radiographic view, vertical alveolar bone loss was observed on both mandibular deciduous canine.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8619-8623
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• 2016

Background: Breast cancer is a common disorder in women, constituting one of the main causes of death all over the world. The purpose of this study was to determine the diagnostic value of the breast tissue diseases by the help of thermography. Materials and Methods: In this paper, we applied non-contact infrared camera, INFREC R500 for evaluating the capabilities of thermography. The study was conducted on 60 patients suspected of breast disease, who were referred to Imam Khomeini Imaging Center. Information obtained from the questionnaires and clinical examinations along with the obtained diagnostic results from ultrasound images, biopsies and thermography, were analyzed. The results indicated that the use of thermography as well as the asymmetry technique is useful in identifying hypoechoic as well as cystic masses. It should be noted that the patient should not suffer from breast discharge. Results: The accuracy of asymmetry technique identification is respectively 91/89% and 92/30%. Also the accuracy of the exact location of identification is on the 61/53% and 75%. The approach also proved effective in identifying heterogeneous lesions, fibroadenomas, and intraductal masses, but not ISO-echoes and calcified masses. Conclusions: According to the results of the investigation, thermography may be useful in the initial screening and supplementation of diagnostic procedures due to its safety (its non-radiation properties), low cost and the good recognition of breast tissue disease.

• Fisheries and Aquatic Sciences
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• v.19 no.10
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• pp.44.1-44.8
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• 2016

Rockfish, Sebastes schlegelii (mean length $14.53{\pm}1.14cm$ and mean weight $38.36{\pm}3.45g$), were exposed for 4 weeks with the different levels of ammonia in the concentrations of 0, 0.1, 0.5, and 1.0 mg/L at 19 and $24^{\circ}C$. The indicators of growth performance such as daily length gain, daily weight gain, condition factor, and hematosomatic index were significantly reduced by the ammonia exposure and high temperature. The ammonia exposure induced a significant decrease in hematological parameters, such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb), and hematocrit (Ht), whose trend was more remarkable at $24^{\circ}C$. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also notably decreased by the ammonia exposure. Blood ammonia concentration was considerably increased by the ammonia concentration exposure. In the serum components, the glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) were substantially increased by the ammonia exposure, whereas total protein was significantly decreased. But, the calcium and magnesium were not considerably changed.

• Journal of Radiation Industry
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• v.10 no.1
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• pp.21-27
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• 2016

Periodontitis is disease of damaged gum tissue that is not removed the plaque onto teeth. In case that the symptoms of disease get pain worse, it will have to extract tooth because of tumefy or bleeding at gums so treatment of drug was required to periodontitis. In this study, the hydrogel was prepared by including superior viscous, excellent elastic, and biocompatibility of Poly(vinyl alcohol, PVA) and antimicrobial drug of Metronidazole (MD). The 15 wt% PVA was dissolved in deionized water and then prepared PVA solution was irradiated using gamma-ray at 25 kGy ($10kGy\;hr^{-1}$). In addition, PVA hydrogel was immersed in each 0.1, 0.25 and 0.5 wt% MD solution using stirrer for 24 hr. The result of the gelation, 0.5 wt% MD loaded PVA hydrogel(76%) was lower than PVA hydrogel (88.2%). The swelling ration of 0.5 wt% MD loaded PVA hydrogel (294.8%) was higher than PVA hydrogel (105.2%). The compressive strength and thermal properties of MD loaded PVA hydrogel was gradually lower. The drug release test of 0.5 wt% MD loaded PVA hydrogel (61%) was higher than 0.1 wt% MD loaded PVA hydrogel (12%). Therefore, MD loaded PVA hygrogel may be a promising tool for periodontitis medicine by gamma-ray.

• Korean Journal of Pharmacognosy
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• v.50 no.4
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• pp.272-276
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• 2019

Corylopsis gotoana has been widely used as a traditional medicine for the treatment of lots of disease including cold, edema and vomiting. However pharmacological and phytochemical studies on the C. gotoana are extremely limited. Here in this study, the author investigated the anti-nociceptive effects of the methanolic extract of Corylopsis gotoana (MCG) using various pain models. In the present study, MCG exhibited strong and dose-dependent anti-nociceptive activities on various experimental pain models including thermal nociception and chemical nociception, compared to positive control such as tramadol and indomethacin. In addition, the result from combination test using naloxone, analgesic activity of MCG was slightly reduced, indicating that MCG acts as a partial opioid receptor agonist. These results demonstrated that MCG has potent analgesic potential and thus it may be possibly used as a valuable anti-nociceptive agent.

• Journal of Oriental Medical Thermology
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• v.6 no.1
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• pp.32-38
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• 2008

Objective: To investigate the changes of facial temperature by Miso Facial Rejuvenation Acupuncture treatment. Methods: One middle-aged women who has no other disease was recruited. The Miso Facial Rejuvenation Acupuncture was performed on only right face. We measured the facial temperature using the Digital Infrared Thermal Imaging (DOREX Inc. Spectrum 9000 MB, USA, D.I.T.I.) before, immediate after and 10 minutes after treatment. And we used the Wilcoxon signed rank test (P<0.05) to compare the difference of facial temperature at each time. Results: Right facial temperature (the area treated by acpuncuture) increased immediately from $30.02{\pm}1.87^{\circ}C$ to $32.24{\pm}1.03^{\circ}C$, the change of temperature is statistically significant. At 10 minutes after treatment, right facial temperature decreased little, but there was no statistical significance. Left facial temperature increased little, but there was no statistical significance. The difference between right and left face increased after Miso facial rejuvenation acupuncture treatment. Conclusion: Miso facial rejuvenation acupuncture could make the facial temperature increase.

• The Journal of Internal Korean Medicine
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• v.21 no.5
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• pp.813-817
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• 2000

Objectives : This study had been done to evaluate the effectiveness of DITI about hemiparesis in 55 patients with cerebral vascular disease who admitted to Dong-seo oriental hospital from November. 10. 1999 to October. 13. 2000, Methods : 55 stroke patients who had hemiparesis not only numbness nor quadriparesis were taken picture with DITI to measure infrared streamed from body's surface. And we compared DITI' results with hemiparesis to evaluate the effectiveness of DITl. Results : There was significant results between DITI and hemiparesis. Above 80%, we could find abnormality in the DITI pictures, which were exactly corresponding to symptom of hemiparesis. Conclusions : We thought that we can use DITI' results as a evaluation of stroke patients with hemiparesis.

• Journal of Acupuncture Research
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• v.20 no.4
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• pp.220-229
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• 2003

Objective : Cervical myelopathy is one of the most severe disease, as well as a common diesaes. However, its clinical study and report about efficiency of oriental treatment are absolutely insufficient. Therefore, we report the treatment about 1 case of Cervical myelopathy like followings. Methods : From November 27th, 2002 to January 11th, 2003, we observed patient hospitalized because of cervical myelopathy, and for its valuation, JOA score(Japanese Orthopedic Association score), Nutric grading, VAS(visual analog scale), and D.I.T.I(Digital Infrared Thermal Imaging)were used. Results : After being hospotalized for 50 days, the patients' JOA score, Nuric grading system, and VAS were improved each from 7 to 13, from grade IV to grade II, and from 8 to 2. Conclusions : We considered that oriental treatment has an useful effect on cervical myelopathy patients' treatment and recovery. But continuous studies, such as comparative studies accompanied by more treatment case.