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Effect of His192 Mutation on the Activity of Alginate Lyase A1-III from Sphingomonas Species A1

  • Yoon, Hye-Jin;Choi, Yong-Jin;Osamu Miyake;Wataru Hashimoto;Kousaku Murata;Bunzo Mikami
    • Journal of Microbiology and Biotechnology
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    • 제11권1호
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    • pp.118-123
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    • 2001
  • The alginate lyase A1-III gene of Sphingomonas species A1 is composed of 1,077 nucleotides, encoding a protein (359 amino acids) with a molecular mass of 40,322 Da. Recombinant A1-III expressed in Escherichia coli exhibited the same full enzymatic activity as native A1-III. In order to identify the critical residue for activity, a site-directed mutation was introduced into the A1-III gene (H192A, His192->Ala). Recombinant A1-III (H192A) exhibited a significant decrease in enzyme activity (one-thirty thousandth of that of A1-III), without any conformational change, as detected by the CD spectra in the far UV region. Also, the chemical modification of wild-type A1-III with methyl 4-nitro benzene sulfonate resulted in a 40% decrease from the initial activity, whereas the same modification of A1-III (H192A) produced no change in the activity. The role of His192 on the catalytic process was also explored based on a model of A1-III docked with mannuronic acid into the active site.

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관류홴의 설계인자 변화에 따른 리어가이더의 표면압력 특성에 관한 실험적 연구 (Experimental Study on the Surface Pressure Characteristics of a Rear-Guider for the Various Design Factors of a Cross-Flow Fan)

  • 김장권
    • 동력기계공학회지
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    • 제9권3호
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    • pp.50-57
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    • 2005
  • A cross-flow fan is strongly influenced by the various design factors of a rear-guider and a stabilizer. The purpose of this paper is to investigate the effects of a rear-guider and a stabilizer on the surface pressure of a rear-guider in an indoor room air-conditioner using a cross-flow fan. The design factors considered in this paper are a rear-guider clearance, a stabilizer clearance, and a stabilizer setup angle, respectively. The operating condition of a cross-flow fan was controlled by changing the static pressure and flowrate using a fan tester. All surface pressures of a rear-guider are differently distributed according to the stabilizer setup angle, and show a zero value in the flow coefficient, ${\Phi}{\fallingdotseq}0.5$ only of a stabilizer setup angle, $45^{\circ}$. Especially, they show a big negative value in the expansion angle larger than $34^{\circ}$ regardless of a rear-guider clearance, a stabilizer clearance, and a stabilizer setup angle. On the other hand, surface pressures for various stabilizer cutoff clearances are better than those for various rear-guider clearances.

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THE FORCING NONSPLIT DOMINATION NUMBER OF A GRAPH

  • John, J.;Raj, Malchijah
    • Korean Journal of Mathematics
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    • 제29권1호
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    • pp.1-12
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    • 2021
  • A dominating set S of a graph G is said to be nonsplit dominating set if the subgraph ⟨V - S⟩ is connected. The minimum cardinality of a nonsplit dominating set is called the nonsplit domination number and is denoted by ��ns(G). For a minimum nonsplit dominating set S of G, a set T ⊆ S is called a forcing subset for S if S is the unique ��ns-set containing T. A forcing subset for S of minimum cardinality is a minimum forcing subset of S. The forcing nonsplit domination number of S, denoted by f��ns(S), is the cardinality of a minimum forcing subset of S. The forcing nonsplit domination number of G, denoted by f��ns(G) is defined by f��ns(G) = min{f��ns(S)}, where the minimum is taken over all ��ns-sets S in G. The forcing nonsplit domination number of certain standard graphs are determined. It is shown that, for every pair of positive integers a and b with 0 ≤ a ≤ b and b ≥ 1, there exists a connected graph G such that f��ns(G) = a and ��ns(G) = b. It is shown that, for every integer a ≥ 0, there exists a connected graph G with f��(G) = f��ns(G) = a, where f��(G) is the forcing domination number of the graph. Also, it is shown that, for every pair a, b of integers with a ≥ 0 and b ≥ 0 there exists a connected graph G such that f��(G) = a and f��ns(G) = b.

Analysis of Double Stranded DNA-dependent Activities of Deinococcus radiodurans RecA Protein

  • Kim, Jong-Il
    • Journal of Microbiology
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    • 제44권5호
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    • pp.508-514
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    • 2006
  • In this study, the double-stranded DNA-dependent activities of Deinococcus radiodurans RecA protein (Dr RecA) were characterized. The interactions of the Dr RecA protein with double-stranded DNA were determined, especially dsDNA-dependent ATP hydrolysis by the Dr RecA protein and the DNA strand exchange reaction, in which multiple branch points exist on a single RecA protein-DNA complex. A nucleotide cofactor (ATP or dATP ) was required for the Dr RecA protein binding to duplex DNA. In the presence of dATP, the nucleation step in the binding process occurred more rapidly than in the presence of ATP. Salts inhibited the binding of the Dr RecA protein to double-stranded DNA. Double-stranded DNA-dependent ATPase activities showed a different sensitivity to anion species. Glutamate had only a minimal effect on the double-stranded DNA-dependent ATPase activities, up to a concentration of 0.7 M. In the competition experiment for Dr RecA protein binding, the Dr RecA protein manifested a higher affinity to double-stranded DNA than was observed for single-stranded DNA.

Three-dimensional evolution of a solar magnetic field that emerges, organizes and produces a flare and flare-associated eruptions of a flux rope and plasmoid

  • Magara, Tetsuya
    • 천문학회보
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    • 제40권1호
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    • pp.63.2-63.2
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    • 2015
  • Solar flare is one of the energetic phenomena observed on the Sun, and it is often accompanied with eruptions such as global-scale eruption of a flux rope (filament/prominence eruption) and small-scale eruption of a plasmoid. A flare itself is a dissipative phenomenon where accumulated electric current representing free magnetic energy is dissipated quickly at a special location called a current sheet formed in a generally highly conductive solar corona. Previous studies have demonstrated how a solar magnetic field placed on the Sun forms a current sheet when magnetic shear is added to the field. Our study is focused on a self-consistent process of how a subsurface magnetic field emerges into the solar atmosphere and forms a current sheet in the corona. This study also gives light to a relation among a flare and two types of flare-associated eruptions; flux-rope eruption and plasmoid eruption.

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자기 변형 작동기를 이용한 진동 절연 시스템 (The vibration isolating system using a magnetostrictive actuator)

  • 정학근;박기환
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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    • pp.276-279
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    • 1997
  • When a magnetostrictive material is exposed to a magnetic field, its geometry changes due to a magnetostrictive effect. The magnetostriction is analogous to the piezoeletricity. The displacement of the magnetostrictive material is proportional to the applied current while that of the piezoelectric material is proportional to the voltage. A magnetostrictive material generates large displacement and higher compressive force compared with a piezoeletric material. These advantages provide a good performance of a vibration isolation of a platform. In this work, it is applied to a driving actuator for vibration isolation of a platform. The properties of a magnetostrictive material are investigated in terms of hysteresis and displacement vs. applied current for a various preload. Modeling of the displacement of the vibration isolating actuator is performed as it behaves as a flow source. A sliding mode controller is designed to demonstrate the ability of the magnetostrictive actuator to reduce the vibration at the platform. The effectiveness of the proposed scheme is demonstrated through experimental works. The experimental results of the vibration of the platform axe presented in terms of time response and frequency response.

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ON A QUASI-SELF-SIMILAR MEASURE ON A SELF-SIMILAR SET ON THE WAY TO A PERTURBED CANTOR SET

  • Baek, In-Soo
    • 한국수학교육학회지시리즈B:순수및응용수학
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    • 제11권1호
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    • pp.51-61
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    • 2004
  • We find an easier formula to compute Hausdorff and packing dimensions of a subset composing a spectral class by local dimension of a self-similar measure on a self-similar Cantor set than that of Olsen. While we cannot apply this formula to computing the dimensions of a subset composing a spectral class by local dimension of a quasi-self-similar measure on a self-similar set on the way to a perturbed Cantor set, we have a set theoretical relationship between some distribution sets. Finally we compare the behaviour of a quasi-self-similar measure on a self-similar Cantor set with that on a self-similar set on the way to a perturbed Cantor set.

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NORMS FOR SCHUR PRODUCTS

  • Shin, Dong-Yun
    • 대한수학회논문집
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    • 제12권3호
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    • pp.571-577
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    • 1997
  • We first show that if $\psi : M_n(B(H)) \to M_n (B(H))$ is a $D_n \otimes F(H)$-bimodule map, then there is a matrix $A \in M_n$ such that $\psi = S_A$. Secondly, we show that for an operator space $\varepsilon, A \in M_n$, the Schur product map $S_A : M_n(\varepsilon) \to M_n(\varepsilon)$ and $\phi_A : M_n(\varepsilon) \to \varepsilon$, defined by $\phi_A([x_{ij}]) = \sum^{n}_{i,j=1}{a_{ij}x_{ij}}$, we have $\Vert S_A \Vert = \Vert S_A \Vert_{cb} = \Vert A \Vert_S, \Vert \phi_A \Vert = \Vert \phi_A \Vert_{cb} = \Vert A \Vert_1$ and obtain some characterizations of A for which $S_A$ is contractive.

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자동차 에어컨용 냉매인 HFC-134a와 OS-12a의 성능 특성에 관한 연구 (A Study on Characteristics of HFC-l34a and OS-l2a Refrigerant in Automobile Air-Conditioning System)

  • 이종인;하옥남
    • 한국자동차공학회논문집
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    • 제10권3호
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    • pp.136-142
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    • 2002
  • HFC-134a is currently used as the refrigerant in automobile air-conditioner, replacing the ozone depleting refrigerant CFC-12. Although HFC-l34a has no ozone depletion potential, it has a relatively high global warming potential, approximately 1300 tins that of CO$_2$ over a 100 year time horizon. Therefore, HFC- l34a does not seem to be a perfect alternative refrigerant due to high GWP. For this reason, non-azeotrope refrigerant mixture have been proposed as a long-term and drop-in alternative to HFC-l34a in the automobile air-conditioning system which has variable operating conditions with changes in RPM and pressure ratio. In this study,OS-l2a of which thermodynamic properties are similar to those of HFC-l34a is selected among the mixed refrigerant. HFC-l34a and OS-l2a are examined experimently by the performance test in the same automobile air-conditioning system.

SOME RESULTS ON THE LOCALLY EQUIVALENCE ON A NON-REGULAR SEMIGROUP

  • Atlihan, Sevgi
    • 대한수학회논문집
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    • 제28권1호
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    • pp.63-69
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    • 2013
  • On any semigroup S, there is an equivalence relation ${\phi}^S$, called the locally equivalence relation, given by a ${\phi}^Sb{\Leftrightarrow}aSa=bSb$ for all $a$, $b{\in}S$. In Theorem 4 [4], Tiefenbach has shown that if ${\phi}^S$ is a band congruence, then $G_a$ := $[a]_{{\phi}^S}{\cap}(aSa)$ is a group. We show in this study that $G_a$ := $[a]_{{\phi}^S}{\cap}(aSa)$ is also a group whenever a is any idempotent element of S. Another main result of this study is to investigate the relationships between $[a]_{{\phi}^S}$ and $aSa$ in terms of semigroup theory, where ${\phi}^S$ may not be a band congruence.