• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.240-247
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• 2001

The effect of microsparged aeration in mammalian cell bioreactor on the oxygen transfer rate and cell viability was studied. The microspargers with differ- ent micron-sized pores were used to supply oxygen to the medium. The oxygen transfer coefficients (k$_{L}$a) measured in the bioreactor were markedly increased, which is due to the increase of the contacting area between air bubbles and liquid medium when the pore size of microsparger decreases. When the impellers of two different types (square-pitch marine impeller and $45^{\circ}$ pitched flat blade impeller) were used for agitation, the k$_{L}$a values were slightly higher with the marine impeller than with the blade impeller. The detrimental effect of direct gas sparging with microsparger on mammalian cells was investigated in bubble columns with various air flow rates and different pore sized microspargers. The first-order cell death rate constant ($k_{d}$ /7) was shown to be directly proportional to the air flow rate and inversely proportional to the pore size. During the cultivation of hybridoma cells using microsparger with the pore size of $0.57\mu$m in the mammalian cell culture bioreactor, the continuous sparging caused the cell death and suppressed the cell growth. However, cells grew normally and cell viability was maintained above 90% in the logarithmic phase when the air was intermittently sparked in order to maintain the dissolved oxygen level above 20%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.456-461
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• 2018

Carotid artery stenting (CAS) has emerged as an alternative treatment for carotid stenosis in patients poorly suited for endarterectomy. Intracerebral hemorrhage following carotid revascularization (endarterectomy, angioplasty, artery stenting) is rare and thought to be related to reperfusion injury in most cases. Early experience suggests an increased incidence of hemorrhage following CAS as compared to endarterectomy. In this study, data were obtained through a case report on an 80-year-old male patient with cerebral infarction. The 80-year-old hypertensive man developed sudden monoparesis in the left arm. He underwent CAS for 90% stenosis of the left proximal internal carotid artery. Brain CT after procedure showed acute hematoma with left posterior cerebral artery territorial hemorrhage, including the upper thalamus with extended intraventricular hemorrhage (IVH). Since this hemorrhage occurred in vascular territory unlikely to have been supplied by the treated artery, this case suggests that the mechanism of intracerebral hemorrhage following CAS may in some cases be different from hyperperfusion hemorrhage classically described following endarterectomy.

• Journal of the Korean Arthroscopy Society
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• v.8 no.1
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• pp.26-30
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• 2004

Purpose: We analyzed the clinical results of ACL(anterior cruciate ligament) reconstruction of chronic anterior cruciate ligament injuries with a fresh frozen Achilles allograft, retrospectively. Methods: Thirty-eight patients(average age 32.4 years) who had an chronic injury of ACL reconstructed with an Achilles allograft between January 1999 and February 2001 were included. The mean follow-up was 19.2(12-36) months. The clinical evaluation was done by range of motion, Lachman and pivot-shift test, KT-1000 arthrometer, Lysholm knee score, and the modified Feagin score. Results: All patients recovered full range of motion of affected knees. With use of Lachman and pivot-shift test data, thirty seven(97.4%) were functional. When comparing KT-1000, we found mean maximum side-to-side differences less than 2.1 mm. According to Lysholm knee score, mean postoperative score was 90.4. Thirty four(89.5%) had good to excellent results in modified Feagin score. Conclusion: Results of ACL reconstruction using fresh frozen Achilles allografts were comparable to autografts. ACL reconstruction with Achilles allograft could be a reasonable altemative to autografts.

• The Journal of Korean Academy of Prosthodontics
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• v.52 no.3
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• pp.195-201
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• 2014

Purpose: This study was designed to compare the amount of polymerization shrinkage of dual-cure resin cements according to different polymerization modes and to determine the effect of light activation on the degree of polymerization. Materials and methods: Four kinds of dual-cure resin cements were investigated: Smartcem 2, Panavia F 2.0, Clearfil SA Luting and Zirconite. Each material was tested in three different polymerization modes: self-polymerization only, immediate light polymerization and 5 minutes-delayed light polymerization. The time-dependent polymerization shrinkage-strain was evaluated for 30 minutes by Bonded-disk method at $37^{\circ}C$. Five recordings of each material with three different modes were taken. Data were analyzed using one-way ANOVA and multiple comparison Scheffe′test (${\alpha}$=.05). Results: All materials, except Panavia F 2.0, exhibited the highest polymerization shrinkage-strain through delayed light-activated polymerization. No significant difference between light activation modes was found with Panavia F 2.0. All materials exhibited more than 90% of polymerization rate in the immediate or delayed light activated group within 10 minutes. Conclusion: As a clinical implication of this study, the application of delayed light activation mode to dual-cure resin cements is advantageous in terms of degree of polymerization.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.534-539
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• 2014

This study was carried out to establish seed propagation method of Hydrangea petiolaris Siebold & Zucc., one of the specific and protected plant species for the floral region. Seed size ranged $1.36{\times}0.84mm$, weight of thousand seeds was $2.3{\pm}0.02mg$. Observation of seed morphology characters showed wrinkled surface and elliptical shape, indicating amber color and fine seeds. Moisture contents of seeds, increased rapidly by water-soaking treatment, recording maximum moisture contents (31.9%) after 48 hours. Percent germination of seeds was higher under the light than dark condition. Seed germination was the best at $25^{\circ}C$ under the light condition (78.0%) among temperature and light conditions treated. Percent germination, germination energy and $T_{50}$ was greatly improved by soaking in $GA_3$ solutions for 48 hours. Especially, $100{\sim}500mg{\cdot}L^{-1}$ $GA_3$ treatment resulted in the highest germination rate as 90.0% and above.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.12
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• pp.883-888
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• 2013

Coagulation and sedimentation tests were conducted with clay and three different coagulants of soil origin (AC-A, AC-B, AC-C) to determine optimal coagulant types and doses to remove algae in stagnant water bodies such as reservoirs. Raw water had an algal density of 2,950 cells/mL and was dominated by Cyanobacteria. Removal rates of algal density by clay (50 mg/L) were 49% and 85% after 10 and 30 minutes sedimentation, respectively. Other natural coagulants achieved 80-90% removal in 10 minutes and 89-94% removal in 30 minutes of sedimentation after adding 20 mg/L each. AC-A was the optimal coagulant from this study considering algal removal rates and other water quality parameters such as turbidity and pH. For the same removal rates of algae, raw waters with higher algal densities required higher coagulant doses although no strong corelation was observed. The coagulants of soil origin did not impact orgnic contents and pH of raw water, but remove phosphate up to 70%.

• Clinics in Shoulder and Elbow
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• v.13 no.2
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• pp.181-187
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• 2010

Purpose: To determine clinical results for arthroscopic repair of a full-thickness rotator cuff tear using a suture bridge technique. Materials and Methods: Between November, 2007 and October, 2008, we evaluated 90 cases of arthroscopic middle, large rotator tear cuff repair. The mean follow-up period was 15 months (range, 12-23 months). Forty-three cases had medium-sized tears; 47 cases had large-sized tears. At the preoperative stage and again at last follow-up, functional results were assessed by the KSS, ASES, UCLA and the PVAS (Pain visual analogue score). Results: Pain score improved from 2.56 preoperatively to 0.96 at final follow-up; movement scores improved from 6.94 to 1.70. At. final follow-up, the average UCLA score improved from 17.08 to 31.17 with 31 excellent (34%), 49 good (54%) and 10 poor results (12%). The final UCLA score was 31.47 in the group less than 60 years of age and 30.69 in the group over 61 years of age (p=0.344). The UCLA score was 31.23 in those with medium-sized tears and 31.11 in those with large-sized tears (p=0.924). The UCLA score was 31.10 in non-trauma patients and 31.23 in trauma patients (p=0.929). Conclusion: Arthroscopic repair of a full-thickness rotator cuff tear using a suture bridge technique can produce excellent clinical results. These outcomes are not affected by age or trauma history.

• Journal of Hospice and Palliative Care
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• v.19 no.4
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• pp.310-321
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• 2016

Purpose: The purpose of this study is to examine the level of understanding of hospice palliative care (HPC) and shared decision making (SDM) among middle-aged adults. Methods: Data were collected from 90 middle-aged adults living in D city using a self-reported questionnaire. The SPSS program was used to analyze the data. Results: Among the participants, 76.7% were aware of the HPC while 82.2% of the participants were not aware of SDM. Among all, 85.6% responded positively for needs of HPC, and 77.8% of participants for SDM. Participants with Christian faith (Protestants and Catholics), high income level and present illness were better aware of HPC than others. The most needed services were nursing care and treatment along with systematic counseling and explanation provided by the medical staff. There was also a significant relationship between the HPC factors, gender, income, current health status and current illness status. Participants with high income level were well aware of SDM. Stronger SDM needs were observed among participants with professional jobs or current illness. Conclusion: For continued discussion on HPC and SDM, it is necessary to implement and promote various education programs for medical staff and the public.

• Korean Journal of Social Welfare
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• v.62 no.1
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• pp.343-365
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• 2010

It is a follow-up study on acculturation types and adaptation level to South Korea among North Korean defecting adolescents who participated in the study with the same purpose five years ago(2003). The study of 2003(1st year study) was done with 90 adolescents, out of whom 46 participated in this study(2nd year study). The data from 46 subjects who participated in both 1st and 2nd studies were analyzed. The results on individual acculturation type showed that 40% of the respondents who belonged to 'marginal' or 'separated' types in the 1st study were changed to 'assimilated' or 'integrated' types in the 2nd. Twenty percent changed from 'assimilated' or 'integrated' types in the 1st study to 'marginal' or 'separated' in the 2nd. Furthermore, the higher is the acceptance level of South Korean culture, the lower are their psycho-social problems and the higher is the satisfaction level of daily lives in South Korea. While the higher is the preservation level of North Korean culture, the higher is aggression. Based on the results, the study developed discussion on diverse alternative plans for helping North Korean defecting adolescents in successfully adjusting to the South Korean society.

• Journal of Dental Rehabilitation and Applied Science
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• v.29 no.4
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• pp.337-346
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• 2013

It is necessary to consider the median lingual foramen carefully to prevent the bleeding due to the damage of the sublingual artery for implant surgery. This study is to evaluate the frequency, location, diameter and the number of the Mandibular median lingual foramen regarding gender and age in the CBCT. Sixty two images of cone beam computed tomography (CBCT) whose visited the Chonnam National University Dental hospital from Sept. 2010 to Apr. 2011 were evaluated. Frequency, number, location and the diameter of Mandibular median lingual foramen shown in the CBCT image were evaluated. Sixty two patients (100%) had at least one median lingual foramen and fifty six patients (90.32%) had multiple foramens. Forty patients (66.13%) showed the median lingual foramen on the location between Mn. central incisors. The mean vertical position of the genial spine and the median lingual foramen was 24.21 mm and 14.52 mm, respectively. And the relative mean vertical dimension of median lingual foramen was 0.45. The mean diameter of the foramen was 0.93 mm. CBCT demonstrated the frequency, location, diameter and the number of median lingual foramen. It is necessary to take CBCT before implant placement to prevent the bleeding.