• Title/Summary/Keyword: 8-domain

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LIR motifs and the membrane-targeting domain are complementary in the function of RavZ

  • Park, Sang-Won;Jun, Yong-Woo;Jeon, Pureum;Lee, You-Kyung;Park, Ju-Hui;Lee, Seung-Hwan;Lee, Jin-A;Jang, Deok-Jin
    • BMB Reports
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    • v.52 no.12
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    • pp.700-705
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    • 2019
  • The bacterial effector protein RavZ is secreted by the intracellular pathogen Legionella pneumophila and inhibits host autophagy through an irreversible deconjugation of mammalian ATG8 (mATG8) proteins from autophagosome membranes. However, the roles of the LC3 interacting region (LIR) motifs in RavZ function remain unclear. In this study, we show that a membrane-targeting (MT) domain or the LIR motifs of RavZ play major or minor roles in RavZ function. A RavZ mutant that does not bind to mATG8 delipidated all forms of mATG8-phosphatidylethanolamine (PE) as efficiently as did wild-type RavZ. However, a RavZ mutant with a deletion of the MT domain selectively delipidated mATG8-PE less efficiently than did wild-type RavZ. Taken together, our results suggest that the effects of LIR motifs and the MT domain on RavZ activity are complementary and work through independent pathways.

Domain Mapping using Nonlinear Finite Element Formulation

  • Patro, Tangudu Srinivas;Voruganti, Hari K.;Dasgupta, Bhaskar;Basu, Sumit
    • International Journal of CAD/CAM
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    • v.8 no.1
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    • pp.29-36
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    • 2009
  • Domain mapping is a bijective transformation of one domain to another, usually from a complicated general domain to a chosen convex domain. This is directly useful in many application problems like shape modeling, morphing, texture mapping, shape matching, remeshing, path planning etc. A new approach considering the domain as made up of structural elements, like membranes or trusses, is developed and implemented using the nonlinear finite element formulation. The mapping is performed in two stages, boundary mapping and inside mapping. The boundary of the 3-D domain is mapped to the surface of a convex domain (in this case, a sphere) in the first stage and then the displacement/distortion of this boundary is used as boundary conditions for mapping the interior of the domain in the second stage. This is a general method and it develops a bijective mapping in all cases with judicious choice of material properties and finite element analysis. The consistent global parameterization produced by this method for an arbitrary genus zero closed surface is useful in shape modeling. Results are convincing to accept this finite element structural approach for domain mapping as a good method for many purposes.

2D Finite Difference Time Domain Method Using the Domain Decomposition Method (영역분할법을 이용한 2차원 유한차분 시간영역법 해석)

  • Hong, Ic-Pyo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.5
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    • pp.1049-1054
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    • 2013
  • In this paper, two-dimensional(2-D) Finite Difference Time Domain(FDTD) method using the domain decomposition method is proposed. We calculated the electromagnetic scattering field of a two dimensional rectangular Perfect Electric Conductor(PEC) structure using the 2-D FDTD method with Schur complement method as a domain decomposition method. Four domain decomposition and eight domain decomposition are applied for the analysis of the proposed structure. To validate the simulation results, the general 2-D FDTD algorithm for the total domain are applied to the same structure and the results show good agreement with the 2-D FDTD using the domain decomposition method.

Design of Sigma Filter in DCT Domain and its application (DCT영역에서의 시그마 필터설계와 응용)

  • Kim, Myoung-Ho;Eom, Min-Young;Choe, Yoon-Sik
    • Proceedings of the KIEE Conference
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    • 2004.11c
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    • pp.178-180
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    • 2004
  • In this work, we propose new method of sigma filtering for efficient filtering and preserving edge regions in DCT Domain. In block-based image compression technique, the image is first divided into non-overlapping $8{\times}8$ blocks. Then, the two-dimensional DCT is computed for each $8{\times}8$ block. Once the DCT coefficients are obtained, they are quantized using a specific quantization table. Quantization of the DCT coefficients is a lossy process, and in this step, noise is added. In this work, we combine IDCT matrix and filter matrix to a new matrix to simplify filtering process to remove noise after IDCT in spatial domain, for each $8{\times}8$ DCT coefficient block, we determine whether this block is edge or homogeneous region. If this block is edge region, we divide this $8{\times}8$ block into four $4{\times}4$ sub-blocks, and do filtering process for sub-blocks which is homogeneous region. By this process, we can remove blocking artifacts efficiently preserving edge regions at the same time.

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Object-Based Operating System (OBJECT에 의한 운영체제의 구성에 대한 연구)

  • 이창수
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.8 no.1
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    • pp.23-29
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    • 1983
  • This paper describes object-based operating system to suppeor relability and abstract data type. For reliability, all objects should be accessed through access rights in capability, and the protection domain is provided for all program modules such that efficient domain switching can be achieved. For abstract data type, type manager is provided.

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A Content Analysis of the test of the National Examination for Registration Nurses in Korea over 3 years (간호사 국가고시문제의 내용분석)

  • 서문자;윤순녕;유지수;송지호;최경숙
    • Journal of Korean Academy of Nursing
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    • v.26 no.1
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    • pp.73-93
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    • 1996
  • This study aimed to analyse the test contents of the national examination for the registered nurses (NERN) over 3 years from 1991 to 1993 in Korea. In recent years in Korea, the MCQ(multiple choice question) has been showing to be a highly recognized method for assessing the qualification of registered nurses. Unfortunately, nursing faculties have found NERN had some bad MCQs through having evaluation workshop for Some MCQs often provide so many unwriting clues which become a bias of the results, and some items fell into the category of the lower level of educational taxonomy such as isolated recall a fact or data. Frequently the stems of the questions are ambigous, unclear, disputable, esoterical or trivial. Considering those fallacies of the national examination, it is very critical to review the test items to see whether it is of high quality, is more fair, reliable and objective in depth. Therefore, this study was done to provide data for the improvement of the test contents as well as the teachers's assessment skill. For this study, the ad hoc committee was composed of 16 members, including 5 education board members of Korean Academic Nurses Association and 11 nursing faculty members. This committee had one day panel discussion and filled the checklist for this study. The process of analysing data was held over 10 times during 1992-1994. The analysis focussed on educational taxonomy such as cognitive domain(knowledge), psychmotor domain (skill), affective domain(attitude) and the level of learning such as recall, understanding, problems solving, and learning area of theory and practice, and the learning content categorised by nursing process and disease process. The test analysed using difficulty index and the structure of the test items was analysed. The conclusions and suggestion as follows : 1. In learning area, the average ratio of the theory and practice was 1 : 1.1 which was less than 1 : 2 suggested by Korean National Health Institute, and the ratio was different by the 8 leaning subjects of nursing. 2. In category of the educational taxonomy, the knowledge domain was emphasized mostly(79. 7%), the skill domain was 14.9%, and the attitude domain was 5.4% only. 3. In the level of learning, generally, the test items of the level of recall(45.5%) and the understanding(46.3%) were covered almost and the problem solving was 8.1%. 4. In the learning contents, generally, the test items related to nursing process was 67.2% and that of disease process was 32.8%. However, this proportion was different by the 8 leaning subjects. Even though the nursing diagnosis has been emphasized in nursing curricula recently, the test items of this was identified very few. 5. In the structure of the test item, some were not clear, incorrect grammar, unclear description and some have clues to answer. 6. In the item analysis, the non-acceptable level of the difficulty index (means too easy) was 65.7%, and the acceptable level was 33.9%. Considering the results we would like to suggest the followings, 1. Since the test items of knowledge domain was dominant, the test items of the practice domain and attitude domain should be emphasized more. 2. The regular review and analysis of NERN should be arranged in order to improve the quality of the test items which will give influence to the nursing education positively.

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Nucleotide Polymorphisms of Cytochrome P450 Genes in Domestic Garlic Cultivars (국내 재배마늘의 Cytochrome P450 유전자의 염기다형성 분포)

  • Kwon, Soon-Tae;Chung, Jinbo
    • Korean Journal of Plant Resources
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    • v.31 no.5
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    • pp.531-537
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    • 2018
  • This study was carried to survey distribution of the nucleotide polymorphisms in heme-binding (HB) domain, which is highly conserved region between 1,210 and 1,240 bp of cytochrome P450, in domestic garlic cultivars. 120 garlic cultivars collected from Korea were classified into seven HB domain variation based on the nucleotide sequence of the domain. Northern type garlic cultivars, collected from Kyungpook, Chungnam, Chungpook and Kangwon province, showed 51.3% of KP2 type nucleotide sequence, 5'-TTT/GGC/GGT/GGA/CGG/AGA/ATA/TGT/CCT/GGA-3' with coding amino acid FGGGRRICPG, 13.7% of KP1, 11.3% of CP, 8.8% of CM and 5% of KW2 types. Southern type cultivars, collected from Kyungnam province, showed 52.5% of KM type nucleotide sequence, 5'-TTT/GGC/GCA/GGA/CGG/AGA/ATT/TGT/CCT/GGA-3' with coding amino acid FGAGRRICPG, 22.5% of KP2, 5.0% of KW2 and 2,5% of CP type nucleotide sequence. These results showed that Korean garlics were cultivated in highly mixed condition even in the same region.

Comparison of structure, function and regulation of plant cold shock domain proteins to bacterial and animal cold shock domain proteins

  • Chaikam, Vijay;Karlson, Dale T.
    • BMB Reports
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    • v.43 no.1
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    • pp.1-8
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    • 2010
  • The cold shock domain (CSD) is among the most ancient and well conserved nucleic acid binding domains from bacteria to higher animals and plants. The CSD facilitates binding to RNA, ssDNA and dsDNA and most functions attributed to cold shock domain proteins are mediated by this nucleic acid binding activity. In prokaryotes, cold shock domain proteins only contain a single CSD and are termed cold shock proteins (Csps). In animal model systems, various auxiliary domains are present in addition to the CSD and are commonly named Y-box proteins. Similar to animal CSPs, plant CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. Cold shock domain proteins have been shown to play important roles in development and stress adaptation in wide variety of organisms. In this review, the structure, function and regulation of plant CSPs are compared and contrasted to the characteristics of bacterial and animal CSPs.

A Study on the Effective Algorithm by Fourier Transform for Numerical Conformal Mapping

  • Song, Eun-Jee
    • Journal of information and communication convergence engineering
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    • v.8 no.3
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    • pp.312-316
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    • 2010
  • Conformal mapping has been a familiar tool of science and engineering for generations. The methods of numerical mapping are usually classified into those which construct the map from standard domain such as the unit disk onto the 'problem domain', and those which construct the map in the reverse direction. We treat numerical conformal mapping from the unit disk onto the Jordan regions as the problem domain in this paper. The traditional standard methods of this type are based on Theodorsen integral equation. Wegmann's method is well known as a Newton-like efficient one for solving Theodorsen equation. An improved method for convergence by applying low frequency pass filter to the Wegmann's method was proposed. In this paper we propose an effective algorithm for numerical conformal mapping based on the improved method. This algorithm is able to determine the discrete numbers and initial values automatically in accordance with the given region and the required accuracy. This results come from analyzing the shape of given domain as seen in the Fourier Transform.