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Production of Glutathione by Candida sp. Mutant (Candida sp. 변이주에 의한 Glutathione 생산)

  • 김대선;유재홍;신원철;윤성식
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.435-439
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    • 1993
  • For the overproduction of glutathione, Candida sp. mutant was isolated by the treatment with U.V. light. The highest glutathione production of Candida sp. mutant was obtained after shaking culture for 48 hours in the cullture medium containing glucose 1.5%(w/v), yeast extract 4.0% (w/v), KH2PO4 0.04%(w/v), biotin 5 ng/ml, and L-cysteine 0.04%(w/v). The optimal pH and temperature for the glutathione production were pH 6.0 and 25C, respectively.

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Padded jacket patterns according to padding thickness for women in their 20s (패딩 두께에 따른 20대 여성용 패딩 재킷 패턴)

  • Lee, Hea-Seung;Suh, Mi-A;Uh, Mi-Kyung
    • The Research Journal of the Costume Culture
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    • v.21 no.5
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    • pp.755-764
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    • 2013
  • This study intends to use the basic patterns in 3oz bodice and 2oz sleeve padded jackets made for women in their 20s to research the proper ease of 2oz bodice and 2oz sleeve, 4oz bodice and 3oz sleeve padded jackets. The study also proposes a method of designing padded jacket patterns according to padding thickness. The results of this study are as follows: The 2oz bodice and 2oz sleeve padded jackets had the following sizes. The front and back bust, waist, and hip circumferences were calculated as B/4+2.5cm and B/4+3cm, W/4+2.6+3.3(D)cm and W/4+1.5+2.6(D)cm, and H/4+2.8cm and H/4+3cm, respectively. The length of the jacket was 62.4cm, and the sleeve length was calculated as 63.4cm. For the 4oz bodice and 3oz sleeve padded jackets, the front and back bust, waist, and hip circumferences were calculated as B/4+4cm and B/4+4cm, W/4+4.1+3(D)cm and W/4+2.5+3.6(D)cm, and H/4+4.3cm and H/4+4cm, respectively. The length of the jacket was 63.2cm, and the sleeve length was calculated as 64.2cm. The results of this study showed that padded jackets with thicker padding need more ease. For jackets with stitches, the decreased lengths must be added in the pattern length. The 2oz bodice and 2oz sleeve, 4oz bodice and 3oz sleeve padded jackets all scored 4 points or higher in the movement functionality assessment, thus showing outstanding movement functionality.

The Study on the Separation of the Subsidiary Elements in Iron and Steel by Using Ion Exchangers (II). The Separation of Anions (이온 교환수지에 의한 철 및 강의 분석에 관한 연구 (제2보). 음이온 성분의 분리)

  • Byoung-Cho Lee;Myon-Yong Park;Kee-Chae Park
    • Journal of the Korean Chemical Society
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    • v.17 no.6
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    • pp.428-433
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    • 1973
  • The quantitative separations of a mixture containing equal amount of each anion such as Si(IV), As(V), P(V), S(VI), W(VI) and Cr(VI) are carried out by the elution through 20${\times}3.14cm^2$ column of anion exchange resin, Dowex 1${\times}$8. The eluents are a mixture of 0.07 M hydrochloric acid and 0.03 M sodium chloride (pH = 1.30) for Si(IV), As(V) and P(V) species, a mixture of 0.6 M sodium chloride and 0.3 M sodium hydroxide for S(VI), W(VI) and Cr(VI) species, and 0.1 N sodium sulfite (pH = 3.48) for P(V) and As(V) species. The subsidiary anions in a standard mixture such as Si(IV), As(V), S(VI), P(V) and W(VI) are separated together from large amount of Fe(III) by the elution through 30cm${\times}3.14cm^2$ column of the resin, Dowex${\times}$50w${\times}$12, using a mixture of 0.1 M sodium nitrate and 2 percent dimethylsulfoxide aqueous solution as an eluent. Si(IV), As(V), S(VI), P(V) and W(VI) eluted together are separated quantitatively under the same conditions as in the separations of the anion mixture. By the conditions obtained in the separations of the standard mixture, Fe(III) and all of the subsidiary anions in steel are quantitatively separated.

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Studies of Oxo-Nitrosyl Complexes(Ⅱ) Synthesis and Properties of Tungsten Oxo-Nitrosyl Complexes with Butylamidoxime Derivatives (산소-니트로실 착물의 연구 (제2보) 부틸아미드옥심 유도체의 텅스텐 산소-니트로실 착물의 합성과 특성)

  • Roh, Soo Gyun;Oh, Sang Oh
    • Journal of the Korean Chemical Society
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    • v.39 no.11
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    • pp.856-862
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    • 1995
  • The tungsten oxo-nitrosyl complexes (n-Bu4N)2[W5O12(NO)2{RC(NH2)NHO}2{RC(NH)NO}2] (R=(CH3)2CH, CH3CH2CH2, CH3SCH2) have been synthesized by the reactions of polyoxotungsten complex (n-Bu4N)2[W6O19] and tungsten dinitrosyl monomeric complex [W(NO)2(acac)(CH3CN)2](BF4) with butylamidoxime derivatives. The prepared complexes have been characterized by elemental analysis, infrared, UV-visible, 1H NMR and 13C NMR spectroscopy. The pentanuclear species was formed by the interaction of the electron-withdrawing {W(NO)2}2+ unit between the two dinuclear tungsten {W2O5}2+ cores. We can estimate to exist large proton interactions viewed from the four doublet in 1H NMR spectrum of (n-Bu4N)2[W5O12(NO)2{(CH3)2CHC(NH2)NHO}2{(CH3)2CHC(NH)NO}2]. We also drew informations of the two different coordination mode and symmetry of the complexes because two ligands appear in 13C NMR spectra instead of four. The {W(NO)2}2+ unit has been cis-form and C2v symmetry in geometric structure.

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The Study on Compartment Fire Experiment According to Fire Load (화재하중에 따른 구획화재 실험 연구)

  • Kweon, Oh-Sang;Kim, Heung-Youl
    • Fire Science and Engineering
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    • v.31 no.6
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    • pp.16-22
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    • 2017
  • In Korea, performance-based fire safety designs are being discussed to deal with the various risks of fire in complex and diverse types of structure. However, performance-based fire safety designs are not actively employed because it is difficult to estimate the fire characteristics related to the various factors in buildings. In this study, real scale fire tests were conducted based on fire severity levels and fire loads provided in He New Zealand Building Code, in order to use the results as guidelines and fundamental data for performance-based designs. In the real scale fire tests conducted in a 10MW full-scale calorimeter, wood cribs were placed in a $2.4(L){\times}3.6(W){\times}2.4(H)m$ mock-up of a compartment which had one $0.8(L){\times}2.0(H)$ opening for different fire loads and heating was continued until all of the wood cribs were burned down. The heat release rate started to increase rapidly 90 seconds after the wood cribs caught fire. In the test with a fire load level 1, the maximum heat release rate of 4743.4 kW was reached at 244 second. In the test with fire load level 2, a maximum heat release rate of 5050.9 kW was reached at 497 second. In the test with fire load level 3, a maximum heat release rate of 4446.9 kW was reached at 677 second.

Effect of Monensin and Fish Oil Supplementation on Biohydrogenation and CLA Production by Rumen Bacteria In vitro When Incubated with Safflower Oil

  • Wang, J.H.;Choi, S.H.;Yan, C.G.;Song, M.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.221-225
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    • 2005
  • An in vitro study was conducted to examine the effect of monensin or fish oil addition on bio-hydrogenation of $C_{18^-} unsaturated fatty acids and CLA production by mixed ruminal bacteria when incubated with safflower oil. Commercially manufactured concentrate (1%, w/v) with safflower oil (0.2%, w/v) were added to mixed solution (600 ml) of strained rumen fluid and McDougalls artificial saliva (control). Monensin $Rumensin^{(R)}$, 10 ppm, w/v, MO), mixed fish oil (0.02%, w/v, absorbed to 0.2 g alfalfa hay, FO) or similar amounts of monensin and fish oil (MO+FO) to MO and FO was also added into the control solution. All the culture solutions prepared were incubated in the culture jar anaerobically at $39^{\circ}C$ up to 12 h. Higher pH (p<0.047) and ammonia concentration (p<0.042) were observed from the culture solution containing MO at 12 h incubation than those from the culture solutions of control or FO. The MO supplementation increased (p<0.0001-0.007) propionate proportion of culture solution but reduced butyrate proportion at 6 h (p<0.018) and 12 h (p<0.001) of incubations. Supplementation of MO or MO+FO increased (p<0.001) the proportions of $C_{18:2}$. The MO alone reduced (p<0.022-0.025) the proportion of c9,t11-CLA compared to FO in all incubation times. The FO supplementation increased the proportion of c9,t11-CLA. An additive effect of MO to FO in the production of c9,t11-CLA was observed at 6 h incubation. In vitro supplementation of monensin reduced hydrogenation of $C_{18^-}$UFAs while fish oil supplementation increased the production of CLA.

Isolation and Characterization of an Alkaline Cellulase Produced by Alkalophilic Bacillus sp. HSH-810 (알칼리성 Cellulase를 생산하는 호알칼리성 Bacillus sp. HSH-810의 분리 및 효소 특성)

  • 김지연;허성호;홍정화
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.139-146
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    • 2004
  • A bacterium producing alkaline cellulase was isolated from soil, leaf mold and compost, and was identified as alkalophilic Bacillus sp. HSH-810 by morphological, cultural and biochemical determination. The optimum cul-ture condition of Bacillus sp. HSH-810 for the growth and alkaline cellulase production was $30^{\circ}C$ and pH 10.0. The maximum alkaline cellulase production was obtained when 1.0%(w/v) CMC, 0.5%(w/v) peptone, 0.02%(w/v) $CaCl_2$ and 0.02(w/v) $CoCl_2$ were used as carbon source, nitrogen source and mineral source, respectively. The optimum pH and temperature of the enzyme activity were pH 10.5 and $50^{\circ}C$, respectively. This enzyme was fairly stable in the pH range of 6.0-13.0 and at $50^{\circ}C$. For the effect of surfactants, the activity of alkaline cellulase was stable in the presence of sodium-$\alpha$-olefin sulfonate (AOS), sodium dodecyl sulfonate (SDS), Tween 20 and Tween 80, but inhibited by the presence of 0.1 linear alkyl-benzene sulfonate (LAS) sig-nificantly.

OBSERVATIONS OF $C_3H_2 (2_{12}-1_{01})$ TOWARD THE SAGITTARIUS A MOLECULAR CLOUD

  • LEE C. W.;MINH Y. C.;IRVINE W. M.
    • Journal of The Korean Astronomical Society
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    • v.26 no.1
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    • pp.73-78
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    • 1993
  • We have mapped the $C_3H_2\;2_{12}-1_{01}$ transition line toward the Sgr A molecular cloud on a 1' grid spacing and derived $C_3H_2$ column densities of $3\~7\times10^{14}\;cm^{-2}$ for molecular clouds of Sgr A. The fractional abundances of $C_3H_2$ relative to $H_2$ are obtained to be $3\~6\times10^{-9}$, which are slightly lower than that for the cold dark cloud TMC-1 but are enhanced by factors of 5-60 compared to those for Sgr B2 and the Orion extended ridge. We also estimate from the $C_3H_2$ column densities total masses of $\~10^6\; M_\bigodot$ for two clouds (M - 0.13 - 0.08 and M - 0.02 - 0.07), which are thought to be close to the virial equilibrium. We suggest that the large abundance of $C_3H_2$ in Sgr A may be partly due to the activities of the Galactic center.

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Design and Fabrication of Triple Band Antenna Applicable to GPS/DCS/WLAN System (GPS/DCS/WLAN 시스템에 적용 가능한 삼중대역 안테나 설계 및 제작)

  • Kim, Min-Jae;Park, Sang-Wook;Yoon, Joong-Han
    • The Journal of the Korea institute of electronic communication sciences
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    • v.14 no.3
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    • pp.475-482
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    • 2019
  • In this paper, we propose a triple band antenna for GPS / DCS / WLAN system. The proposed antenna has the characteristics required by considering the interconnection of two strip lines and various slits on the ground place. The total substrate size is $31mm(W1){\times}50mm(L1)$, thickness (h) 1.6 mm, and the dielectric constant is 4.4, which is made of $22mm(W7+W12+W8){\times}43mm(L4+L3)$ antenna size on the FR-4 substrate. From the fabrication and measurement results, bandwidths of 340 MHz (1.465 to 1.805 GHz), 480 MHz (2.155 to 2.635 GHz) and 1950 MHz (4.975 to 6.925 GHz) were obtained on the basis of -10 dB. Also, gain and radiation pattern characteristics are measured and shown in the frequency triple band as required.

Design and fAbrication of Triple Band WLAN Antenna Applicable to Wi-Fi 6E Band with DGS (DGS를 갖는 Wi-Fi 6E 대역을 위한 삼중대역 WLAN 안테나 설계 및 제작)

  • Sang-Wook Park;Gi-Young Byun;Joong-Han Yoon
    • The Journal of the Korea institute of electronic communication sciences
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    • v.19 no.2
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    • pp.345-354
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    • 2024
  • In this paper, we propose a triple band WLAN antenna for Wi-Fi 6E band with DGS. The proposed antenna has the characteristics required frequency band and bandwidth by considering the interconnection of two strip lines and three areas on the ground place. The total substrate size is 31 mm (W) × 50 mm (L), thickness (h) 1.6 mm, and the dielectric constant is 4.4, which is made of 22 mm (W6 + W4 + W5) × 43mm (L1 + L2 + L3 + L5) antenna size on the FR-4 substrate. From the fabrication and measurement results, bandwidths of 340 MHz (1.465 to 1.805 GHz) for 900 MHz band, 480 MHz (2.155 to 2.635 GHz) for 2.4 GHz band and 1950 MHz (4.975 to 6.925 GHz) for 5.0/6.0 GHz band were obtained on the basis of -10 dB. Also, gain and radiation pattern characteristics are measured and shown in the frequency triple band as required.