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Correlation between Glycemic Index and in vitro Starch Hydrolysis of Cereals (곡류의 혈당지수와 전분 가수분해율과의 상관관계)

  • Lee, Jung-Sun;Shin, Hyun-Kyung
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1229-1235
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    • 1998
  • To see the correlation between the rate of in vitro starch hydrolysis and the glycemic index, an in vitro digestion was carried out by incubating the cereal samples for 2 hours with ${\alpha}-amylase$ in dialysis tubing. Also the levels of blood glucose were measured over 2 hours after feeding healthy volunteers with 50 g carbohydrate portions. Hydrolysis area, hydrolysis index (HI) and the dialysate content of carbohydrate throughout the digestion time for barley was significantly below those for other cereals (p<0.05), and unpolished glutinous rice was significantly above (p<0.05). The GI-glucose of barley $(57%{\pm}7)$ to glucose as standard was significantly (p<0.05) lower than those of other cereals whereas the GI-glucose of glutinous rice $(110%{\pm}8)$ was significantly higher (p<0.05) than other cereals. The GI-rice values to rice as standard were $122%{\pm}4$ for glutinous sorghum, $116%{\pm}13$ for job's tear, $115%{\pm}13$ for glutinous millet, $106%{\pm}6$ for unpolished glutinous rice, $102%{\pm}7$ for glutinous rice, $100%{\pm}0$ for rice, $90%{\pm}12$ for unpolished rice, $85%{\pm}6$ for foxtail millet, $79%{\pm}5$ for buckwheat and $63%{\pm}6$ for barley. The GI-rice was significantly correlated to hydrolysis area and HI (r=0.75, p<0.01). It suggests that the in vitro starch hydrolysis offers good potential to predict the in vivo glycemic response of starch foods.

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Evaluation of Perfusion and Image Quality Changes by Reconstruction Methods in 13N-Ammonia Myocardial Perfusion PET/CT (13N-암모니아 심근관류 PET/CT 검사 시 영상 재구성 방법에 따른 관류량 변화와 영상 평가)

  • Do, Yong Ho;Lee, Hong Jae;Kim, Jin Eui
    • The Korean Journal of Nuclear Medicine Technology
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    • v.18 no.1
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    • pp.69-75
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    • 2014
  • Purpose: The aim of this study was to evaluate changes of quantitative and semi-quantitative myocardial perfusion indices and image quality by image reconstruction methods in $^{13}N$-ammonia ($^{13}N-NH_3$) myocardial perfusion PET/CT. Materials and Methods: Data of 14 (8 men, 6 women) patients underwent rest and adenosine stress $^{13}N-NH_3$ PET/CT (Biograph TruePoint 40 with TrueV, Siemens) were collected. Listmode scans were acquired for 10 minutes by injecting 370MBq of $^{13}N-NH_3$. Dynamic and static reconstruction was performed by use of FBP, iterative2D (2D), iterative3D (3D) and iterative TrueX (TrueX) algorithm. Coronary flow reserve (CFR) of dynamic reconstruction data, extent(%) and total perfusion deficit (TPD) (%) measured in sum of 4-10 minutes scan were evaluated by comparing with 2D method which was recommended by vendor. The image quality of each reconstructed data was compared and evaluated by five nuclear medicine physicians through a blind test. Results: CFR were lower in TrueX 18.68% (P=0.0002), FBP 4.35% (P=0.1243) and higher in 3D 7.91% (P<0.0001). As semi-quantitative values, extent and TPD of stress were higher in 3D 3.07%p (P=0.001), 2.36%p (P=0.0002), FBP 1.93%p (P=0.4275), 1.57%p (P=0.4595), TrueX 5.43%p (P=0.0003), 3.93%p (P<0.0001). Extent and TPD of rest were lower in FBP 0.86%p (P=0.1953), 0.57%p (P=0.2053) and higher in 3D 3.21%p (P=0.0006), 2.57%p (P=0.0001) and TrueX 5.36%p (P<0.0001), 4.36%p (P<0.0001). Based on the results of the blind test for image resolution and noise from the snapshot, 3D obtained the highest score, followed by 2D, TrueX and FBP. Conclusion: We found that quantitative and semi-quantitative myocardial perfusion values could be under- or over-estimated according to the reconstruction algorithm in $^{13}N-NH_3$ PET/CT. Therefore, proper dynamic and static reconstruction method should be established to provide accurate myocardial perfusion value.

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Relationships of Plasma Insulin-like Growth Factor (IGF)-I and IGF-II Concentrations to Litter Size (Landrace와 Yorkshire 돼지에서 혈장의 Insulin-like Growth Factor (IGF)-I과 IGF-II 농도와 산자수 및 비유성적과의 관계)

  • Lee, C. Y.;Baik, K. H.;Lee, D. H.;Park, H. C.
    • Journal of Animal Science and Technology
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    • v.45 no.1
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    • pp.33-40
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    • 2003
  • The present study was undertaken to find relationships of plasma insulin-like growth factor (IGF)-I and IGF-II concentrations to litter size and lactation performance. Sixty pure-bred Landrace and Yorkshire pigs having similar farrowing weeks which had been selected from a large number of pregnant gilts and sows were divided into low- (<${\mu}$-0.5SD) and high-litter size (>${\mu}$+0.5 SD) lines under a 2 (breed)${\times}$2 (line) factorial arrange of treatments. After adjusting the litter size to nine piglets per sow at farrowing, total litter weight was measured at three weeks postpartum at weaning as an index of milk yield. Blood samples were obtained from the jugular vein at day (d)-90 pregnancy (Px) and at d-15 postpartum. The litter size or the number of piglets born during the present experiment and the average litter size during the entire parities up to the present one were greater in the high-line than in the low-line by 3.7 and 2.4 piglets, respectively (P<0.01); effect of the breed on litter size was not significant. Plasma IGF-II concentration at d-90 Px was greater in the high-line than in the low-line. Litter size and d-90 Px IGF-I concentration were negatively correlated in Landrace (r=-0.46; P<0.05) and tended to be negatively correlated in Yorkshire (r=-0.31; P=0.09), which resulted in a significant negative correlation between these two variables in total animals (r=-0.35; P<0.01). Litter weight at weaning was not different between the two breeds or lines. Relationships between the litter weight and IGF concentration were not consistent across the breed ${\times}$ physiological stage combinations. Results suggest that d-90 Px IGF concentrations may be indicative of the litter size at impending farrowing.

The Effects of Bark on Heavy Metal Adsorption I. The Effects of Pine and Oak Barks on Adsorption of $Fe^{++}$ and $Ni^{++}$ in Wastewater (수피(樹皮)에 의(依)한 중금속(重金屬) 흡착효과(吸着效果) I. 수피(樹皮)를 이용(利用)한 폐수(廢水)중 $Fe^{++}$$Ni^{++}$의 제거(除去) 효과(效果))

  • Kim, Kyung-Jig;Paik, Ki-Hyon
    • Korean Journal of Environmental Agriculture
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    • v.5 no.1
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    • pp.55-60
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    • 1986
  • The objective of this study was to investigate the physical and chemical factors of Pinus densiflora SIEB. et ZUCC. and Quercus mongolica Fisher barks affecting on the adsorption of heavy metals. The results obtained can be summarized as follows: 1. With decreasing the particle size of bark, the adsorption rate of two heavy metal ions were increased. In case of using same particle size, the adsorption of $Fe^{++}$, and $Ni^{++}$ by Quercus bark showed higher than by Pinus bark. 2. The effect of untreated bark on the adsorption of heavy metal was more or less 5% higher than that of HCHO-treated bark in both species. But the color absorbances of the filtrates from HCHO-treated Pinus and Quercus barks were 5.8 and 11.8 times smaller than those of the filtrate from untreated Pinus and Quercus barks, respectively. 3. The maximum adsorption of $Fe^{++}$, and $Ni^{++}$ by bark was shown after 30 min. of the reaction. 4. With increasing the concentration of heavy metal, the amount of adsorption by bark was increased, but the adsorption ratio were decreased. 5. The maximum adsorption of $Fe^{++}$, and $Ni^{++}$ appeared at final pH of $4{\sim}5$, and pH of $3.6{\sim}4.0$ in filtrate, respectively. 6. With increasing the bark weight per a given heavy metal solution, the adsorption ratio were increased, but the amount of adsorption per gram of bark was the highest on the reaction with 2g of bark in a economical sense showing the amount of adsorption of 21mg $Fe^{++}$/g and 7mg $Ni^{++}$/g of Pinus bark, 36mg $Fe^{++}$/g and 9mg $Ni^{++}$/g of Quercus bark, respectively.

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Material Balance and Properties of Compost during Composting of Household Food Wastes Blended with Waste Newspapers (신문지 첨가에 의한 음식쓰레기 퇴비화 과정 중 물질수지 및 퇴비의 성분)

  • Han, Jong-Phil;Park, Ju-Won;Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.51-57
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    • 2000
  • Waste newspapers was used as an amendment to adjust the moisture of household food wastes for composting. The mixture of household food wastes and waste newspapers was composted in an especially designed small home composter, where the early fed composting materials were discharged early. The temperature inside composting materials was influenced very much by that of outside, because the composter was not insulated. Accordingly, the higher the outside temperature was, the higher the decomposition rate was. The temperature inside composting materials did not reach to optimum, because the amount of composting materials added in the composter everyday was too little, and it caused too high water content of discharged compost after 15 weeks. Therefore, it was required that the composter must be insulated to maintain the higher temperature. The inorganic compounds$(K_2O,\;CaO,\;MgO,\;P_2O_5)$ and heavy metals(Zn, Cu, Cr, Cd, Hg, As) were accumulated obviously in produced compost, when dry recycled compost was reused as the amendment for adjusting the moisture.

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Eutrophication in the Upper Regions of Brackish Lake Sihwa with a Limited Water Exchange (물 교환이 제한적인 시화호 상류 기수역의 부영양화)

  • Choi, Kwnag-Soon;Kim, Sea-Won;Kim, Dong-Sup;Heo, Woo-Myoung;Lee, Yun-Kyoung;Hwang, In-Seo;Lee, Han-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.2
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    • pp.216-227
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    • 2008
  • To understand eutrophication in the upper regions of brackish Lake Sihwa with a limited water exchange, temporal and spatial distributions of pollutants in water and sediment were investigated from March to October in 2005 and 2006. Also, pollution levels of water and sediment were estimated by trophic state index (TSI) and sediment quality guideline (SQG). Total nitrogen (TN), total phosphorus (TP), organic matter (COD), and chlorophyll $\alpha$ (Chl-$\alpha$) concentrations in the surface waters were largely varied temporally and spatially, and the variations were highest in the middle areas where strong halocline was formed. Chl-$\alpha$ concentrations in the middle area were very high in April (>$900\;{\mu}g\;L^{-1}$) when algal blooms (red tides) occurred. The relationships between TN and Chl-$\alpha$ (r=0.31), and TP and Chl-$\alpha$ (r=0.65) indicated that the algal growth was primarily affected by phosphorus rather than nitrogen. The distribution of COD was similar to that of Chl-$\alpha$, indicating that the autochthonous organic matters may be a more important carbon source, especially in the middle areas. The brackish water regions were classified as eutrophic or hypertrophic based on their TSI values ($69{\sim}76$). In addition, the content of nutrients (especially TP) in surface sediments were classified as severe polluted state, except the upper areas. Major causes of the eutrophication observed were probably due to high nutrients loading from watersheds, the phosphorus release from anaerobic sediment, and long retention time by the limited water exchange through the sluice gates.

Study of Efficient Production of Cloned Embryos in Porcine 1. Effect of Ethanol, $Ca^{2+}$ Ionophore, 6-DMAP, and Cycloheximide Concentration and Exposure Time on Activation, Cleavage, and In Vitro Development of Porcine Oocytes (효율적인 돼지 복제수정란 생산에 관한 연구 1. Ethanol, $Ca^{2+}$ Ionophore, 6-DMAP, Cycloheximide의 농도와 노출시간이 돼지난자의 활성화와 발달에 미치는 영향)

  • 위갑인;김광현;강만종;문승주
    • Korean Journal of Animal Reproduction
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    • v.27 no.2
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    • pp.103-113
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    • 2003
  • This study was conducted to investigate the optimal condition for produce of large quantity recipient oocytes on porcine cloned embryos. In order to determined the optimum concentration and exposure time of ethanol, $Ca^{2+}$-ionophore, 6-DMAP and cycloheximide, in vitro matured oocytes were activated in TCM-199 containing various chemicals and 15% FBS. The activated oocytes were cultured in Whitten's medium containing 10% FBS at 5% $CO_2$. 1. When the porcine oocytes were activated with the ethanol, the best pronucleus formation, cleavage, and in vitro development rate were obtained in the 10% for 10 minutes, which was significantly higher than all of the other treatment(53.4%, 51.6% and 39.9%, respectively). 2. When the porcine oocytes were activated with the $Ca^{2+}$-ionophore, the pronucleus furmation, cleavage, and in vitro development rate were found significantly higher in the 25$\mu$M fur 2min. treatment than those of other concentration and exposure time(59.7%, 62.2% and 43.9%, respectively). 3. When the porcine oocytes were activated with the 6-DMAP, the best pronucleus formation, cleavage, and in vitro development rate were obtained in the 2mM for 2hr~4.5hr(57.3%, 58.4% and 29.0%, respectively). 4. When the porcine oocytes were activated with the cycloheximide, result showed that pronucleus formation, cleavage, and in vifro development rate were 52.1%, 47.7% and 31.8%, respectively, in the 5$\mu\textrm{g}$/ml for 4hr~6hr treatmrent, which was significantly higher than all of the other treatment. These results suggested that the active condition of porcine oocytes was established by optimum concentration and exposure time among different chemicals for produce of large quantity recipient oocytes.s.

Chemical Components and Antioxidative Effects of Eriobotrya japonica Lindl. Leaf (비파잎의 이화학적 성분과 항산화효과)

  • Hwang, Yun-Gyeong;Lee, Jae-Joon;Kim, Ah-Ra;Lee, Myung-Yul
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1625-1633
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    • 2010
  • This study was carried out to investigate the physicochemical compositions and antioxidative effects of Eriobotrya japonoca Lindl (Loquat). The proximate compositions of the loquat leaf on a dry matter basis were 8.78% moisture content, 6.74% crude protein, 7.87% crude fat, 6.99% crude ash, 43.61% dietary fiber and 26.01% carbohydrate. In analysis of free amino acids, 16 kinds total amino acid components, 17 kinds of components were isolated from loquat. The essential amino acids contained in loquat leaf accounted for 50.15% of total amino acids, while the non-essential amino acids accounted for 49.85%. In analysis of total fatty acids, only 5 kinds of acid were detected: lauric acid, myristic acid, pentadecanoic acid, stearic acid and oleic acid. The contents of vitamin A, vitamin E and vitamin C were 0.039 mg%, 0.096 mg% and 0.575 mg%, respectively. The mineral contents of loquat leaf were greater in order of Zn

Effects of Hepcidin Hormone on the Gene Expression of Ferroportin and Divalent Metal Transporter 1 in Caco-2 Cells and J774 Cells (Caco-2 소장세포와 J774 대식세포에서 Hepcidin 호르몬이 철분 수송체 Ferroportin과 Divalent Metal Transporter 1의 유전자 발현에 미치는 영향)

  • Chae, Sun-Ju;Chung, Ja-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.6
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    • pp.721-728
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    • 2008
  • Hepcidin is a peptide hormone produced by the liver, of which secretion is closely related to iron status in the body. However, little is known about the molecular mechanism(s) by which this peptide regulates body iron homeostasis. The purpose of this study was to determine the effects of hepcidin treatment within the physiological concentration range on the expressions of two different iron transporter proteins-ferroportin (FPN) and divalent metal transporter 1 (DMT1). Differentiated Caco-2 intestinal cells and macrophage J774 cells were treated with either synthetic hepcidin or hepcidin-rich fraction separated from human urine at the concentration of 10 nM and 100 nM for 24 hours. Results show that hepcidin treatment in differentiated Caco-2 cells or in J774 cells did not change the level of either FPN mRNA or DMT1 mRNA. On the other hand, hepcidin treatment at the dose of 100 nM significantly decreased the FPN protein levels and DMT1 protein levels in differentiated Caco-2 cells. Similarly, urinary hepcidin treatment (10 nM & 100 nM) also significantly decreased the levels of FPN and DMT1 proteins in J774 macrophage cells. These results showed that hepcidin might play an important role in the regulation of iron homeostasis by lowering the protein levels of iron transporter FPN and DMT1 both in enterocytes and in macrophage cells.

A DESCRIPTIVE STUDY ON THE CONTRIBUTING FACTORS OF EARLY CHILDHOOD CARIES OF 5 YEARS CHILDREN IN KANGNUNG CITY (강릉시 5세 아동의 "조기 유아기 우식증" 관련 추정요인의 기술 역학적 연구)

  • Park, Jin-A;Ma, Deuk-Sang;Park, Deok-Young;Park, Ho-Won;Lee, Gwang-Su
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.2
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    • pp.226-236
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    • 2002
  • The purpose of this study was to get descriptive statistics of the contributing factors for early childhood caries and to predict the relationship of dietary, behavior factors and health status factors of the mother and child at pregnancy and after birth. 411 first caregivers of 5-year-old children in 12 kindergartens in Kangnung city were selected by stratified random cluster sampling. They were asked to fill out questionnaires and 364 of them responded. The obtained results were as follow: 1. Over the three-Fourth of children used nursing bottle or had breast feeding habit beyond the age of 1 year. 2. 8.7% of respondents didn't recognize the necessity of the preventive measures immediate after eruption of primary tooth, and only 35.1% replied that they had begun tooth cleaning. 3. Over 90% of children brush the teeth more than once per day. But over half (614%) of them brush their teeth without parents instruction. Sixty percent of children eat between the meals as often as 1-3 time(s) a day and the remainder at any times. 4. The first time of dental visit was for most children (87%) at over 3 years, recommending the earlier dental visit. Notwithstanding the rate of routine dental visit experience was relatively high(40.2%), implicating positive parents' attitude about oral health at Kangnung area. 5. The relationships between oral health state of the parents and the variables such as the timing of the first tooth cleaning, the frequency of brushing, the time of first dental visit, and the reason of first dental visit were not statistically significant. Together, there was no statistically significant difference between rural and urban area, private and public kindergarten, and boy and girl($x^2-test$, p>.05 or Fisher's exact test, p>.05).

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