• Bulletin of the Korean Chemical Society
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• v.27 no.7
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• pp.991-994
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• 2006

Herein, insoluble single-walled carbon nanotube (SWNT) was successfully dispersed into water in the presence of a special kind of surfactant-dicetyl phosphate (DCP), subsequently, a SWNT-DCP composite film coated glassy carbon electrode (GCE) was fabricated. The electrochemical behaviors of 6-benzylaminopurine (6-BAP) at the unmodified GCE and SWNT-DCP modified GCE were examined. It is found that the SWNT-DCP modified GCE remarkably enhances the oxidation peak current of 6-BAP, indicating great potential in the determination of trace level of 6-BAP. Finally, a sensitive and simple voltammetric method with a good linear relationship in the range of ${\times}5.0\;\;10^{-8}\sim 2.5\;{\times}\;10^{-6}$ mol/L, was developed for the determination of 6-BAP. The detection limit is as low as $2.0\;{\times}\;10^{-8}$ mol/L for 3-min accumulation. This newly-proposed method was successfully demonstrated with practical samples.

• Journal of Pathology and Translational Medicine
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• v.52 no.6
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• pp.378-385
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• 2018

Background: BRCA1-associated protein 1 (BAP1) mutations are frequently reported in clear cell renal cell carcinoma (ccRCC); however, very few studies have evaluated the role of these mutations in other renal cell carcinoma (RCC) subtypes. Therefore, we analyzed BAP1 protein expression using immunohistochemistry in several RCC subtypes and assessed its relationship with clinicopathological characteristics of patients. Methods: BAP1 expression was immunohistochemically evaluated in tissue microarray blocks constructed from 371 samples of RCC collected from two medical institutions. BAP1 expression was evaluated based on the extent of nuclear staining in tumor cells, and no expression or expression in <10% of tumor cells was defined as negative. Results: Loss of BAP1 expression was observed in ccRCC (56/300, 18.7%), chromophobe RCC (6/26, 23.1%), and clear cell papillary RCC (1/4, 25%), while we failed to detect BAP1 expression loss in papillary RCC, acquired cystic disease-associated RCC, or collecting duct carcinoma. In ccRCC, loss of BAP1 expression was significantly associated with high World Health Organization (WHO)/International Society of Urological Pathology (ISUP) grade (p=.002); however, no significant correlation was observed between loss of BAP1 expression and survival in ccRCC. Loss of BAP1 expression showed no association with prognostic factors in chromophobe RCC. Conclusions: Loss of BAP1 nuclear expression was observed in both ccRCC and chromophobe RCC. In addition, BAP1 expression loss was associated with poor prognostic factors such as high WHO/ISUP grade in ccRCC.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.39-42
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• 2001

C6-Benzylamino purine (BAP) 400 ppm with 500 ppm Spreader, 500 ppm Cover, 250 ppm Spreadersticker, 350 ppm Siloxane, or 1% Tween 20 was sprayed and the effect of surface-active agents on the feathering of maiden apple trees was evaluated. Only Tween 20 showed additional effect on the feathering in comparison with the single BAP treatment. In the second year different concentrations of Tween 20 were added to 400 ppm BAP. Each BAP treatment added with 0.2, 0.5, 0.8, or 1.0% Tween 20 was more effective in feathering than BAP 400ppm alone, but no significant differences among the concentrations.

• Journal of Plant Biology
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• v.37 no.2
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• pp.175-181
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• 1994

해녀콩(Canavalia lineata) 유식물 자엽에서 N-$\alpha$-benzoyl-DL-arginine p-nitroanilide hydrolase(BApNAase)를 부분정제하여 그 성질을 밝혔다. 부분정제한 BApNAase는 purification fold가 77.5이었고 회수율은 7%이었으며 비활성도는 7.75 unit/mg이었다. BApNAase의 분자량은 200 kD이고 젤라틴분해효소 P3인 것으로 밝혀졌으며 최적 pH는 9.5이었다. BApNAase의 Vmax와 Km은 각각 15.5 unit/mg와 1.6 mM로 최대반응속도가 동물의 트립신보다 7배 가량 낮은 반면에 N-$\alpha$-benzoyl-DL-arginine p-nitroanilide(BApNA)에 대한 기질 친화성은 4배 가량 높았다. 또한, BApNAse는 1 mM의 phenylmethanesulfonyl fluoride(PMSF)에 의해 90%나 크게 억제된 반면 aprotinin에 의해서는 크게 억제되지 않아 트립신과는 다른 serine proteinase로 판단되었으며, 효소활성은 Ca2+과 Mg2+에 의해 다소 증가하나 Mn2+, Hg2+, Zn2+에 의해 크게 억제되었다.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.6
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• pp.109-114
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• 2013

The biological active points (BAP) are known as low resistance spots or good electro-permeable points. In this paper, a new method for BAP detection using the bio-impedance measurement system based on the adaptive frequency tracking filter (AFTF) and the transition event detector is presented. Also, the microcontroller process continuous time demodulation of the modulated signal by multi frequency components using the AFTF. The transition event detector based on the phase space method is applied about each frequency using the BAP equivalent model which is proposed.

• Journal of Korean Society for Atmospheric Environment
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• v.6 no.1
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• pp.22-30
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• 1990

The contents of benzo(a)pyrene (Bap.), Pyrene(Py) and Perylene(Pery) were determined by one-dimensional dual band thinlayer chromatogragphy and spectrofluorophotometer in soil samples from road at 36 places of Ulsan and 6 places of Seoul from April to May, 1984. The concents were as follows: 1. Ulsan city : Bap. 0.05-3.66 $\mu$g/g ; mean 1.07 $\mu$g/g Py. 0.60-11.01 $\mu$g/g ; mean 4.34 $\mu$g/g Pery. 0.94-5.48 $\mu$g/g ; mean 1.83 $\mu$g/g 1) Industrial area : Bap. 0.03-3.66 $\mu$g/g ; mean 5.28 $\mu$g/g Py. 2.34-9.07 $\mu$g/g ; mean 5.28 $\mu$g/g Pery. 1.01-3.69 $\mu$g/g ; mean 2.09 $\mu$g/g 2) Commercial area : Bap. 0.15-2.27 $\mu$g/g ; mean 0.29 $\mu$g/g Py. 0.06-11.0 $\mu$g/g ; mean 4.04 $\mu$g/g Pery. 1.03-5.48 $\mu$g/g ; mean 1.84 $\mu$g/g 3) Residential area : Bap. 0.05-0.05 $\mu$g/g ; mean 0.29 $\mu$g/g Py. 1.34-5.53 $\mu$g/g ; mean 2.74 $\mu$g/g Pery. 0.94-2.15 $\mu$g/g ; mean 1.18 $\mu$g/g 2. Commerical area in Seoul : Bap. 0.35-1.01 $\mu$g/g ; mean 0.74 $\mu$g/g Py. 9.37-24,44 $\mu$g/g ; mean 18.64 $\mu$g/g Peay. 1.47-2.24 $\mu$g/g ; mean 1.87 $\mu$g/g 3. The areas that had high contents of Benzo(a)pyrene had also high contents of Pyrene and Perylene. 4. The industrial area had the highest contents of polynuclear aromatic hydrocarbons, followed by commercial area and residential area. 5. The contents of Bap. and Pery. in the commercial area were similar in Seoul and Ulsan, while the content of Py. in Seoul was 4 times higher than in Ulsan.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.47-53
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• 2007

This study was carried out to identify the treatment methods for the inhibition of lateral roots and promotion of hypocotyl enlargement using plant growth regulators for the production of high quality soybean sprouts. Inhibition of lateral root formation was not significant from the $CA_3$ and NAA treatments. From the treatment of BAP, lateral roots were not occurred at all, and hypocotyl enlargement was promoted at the low concentration of $10\;mg{\cdot}L^{-1}\;and\;15\;mg{\cdot}L^{-1}$. Promotion of hypocotyl enlargement was higher as the BAP treatment time was shorter. The optimum time of BAP treatment was 6 hours. Occurrence ratio of lateral roots were low and hypocotyl enlargement was promoted from water supply every 24 hours after BAP treatment. In the indole B treatment, lateral roots occurrence was very low with the concentration of 1.1%, which is much lower than the optimum concentration of 4.2%.

• Journal of Korean Society of Forest Science
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• v.79 no.2
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• pp.109-114
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• 1990

The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (WPM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0/l BAP enhanced shoot development and shoot elongation, whereas addition of 0.5/l BAP was effective on MS medium. A better results were obtained from WPM with 1.0/l BAP and MS with 0.1/l BAP. Developed shoots were subcultured on each basal media but with 0.2/l BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 /l IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan's multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0/l BAP.

• Journal of Life Science
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• v.21 no.7
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• pp.1025-1031
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• 2011

The purpose of this study was to analyze the effects of aerobic exercise and black garlic intake on blood lipids, lipid peroxidation and BAP in rats. The subjects for the study were 25 male Sprague-Dawley rats composed of the control group (CG, n=6), exercise group (EG, n=6), which trained for 4 weeks (30-60 min/day, speed at 15 m/min), black garlic intake group (BGG, n=7), and exercise with black garlic intake group (EBGG, n=6). Blood lipids, lipid peroxidation, and BAP were measured in all the subjects after the end of the 4 week treadmill exercise period. The findings of this study were as follows; TC and TG were significantly lower in the EBGG compared to other groups, while there were no significant findings for HDL-C and LDL-C levels. TBARS was significantly lower in the EBGG compared to the CG, and the BAP from the EBGG and EG were significantly higher than in the CG and BGG after 4 weeks of treadmill exercise. Therefore, aerobic exercise with black garlic intake was effective in improving TC, TG, TBARS, and BAP. Consequently, aerobic exercise with black garlic intake will be effective for activating antioxidant defenses and decreasing cardiovascular diseases.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.32-39
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• 2007

This study was carried out to find the optimum treatment condition for the improving germination using the plant growth regulators with $GA_3$ and BAP, to Kentucky bluegrass and Tall fescue showing low germination. $GA_3$ treatment was more effective on the germination of Kentucky bluegrass than BAP single treatment or the combination treatment of $GA_3$ and BAP, regardless of their treatment concentration. Seed germinability was 6 days treatment was significantly better than that of 3 days treatment in the experiment for treatment period of the plant growth regulators. When seeds were treated with $1000{\mu}M$ of $GA_3$ for 6 days, on the percent germination was improved as much as between 10% and 38% comparing with the control. In Tall fescue seed, the percent germination was not significantly improved, although it was slightly increased when the plant growth regulators were treated. For the optimum condition, on percent germination was improved as much as 10% comparing with the control of Tall fescue treated with $1000{\mu}M$ of $GA_3$ for 3 days. Percent emergence fur the seeds of Kentucky bluegrass and Tall fescue treated $GA_3$ in the field was improved as much as $8{\sim}9%$ comparing with the control, and mean emergence time $(E_{50})$ was also faster for $1{\sim}2$ days than that of the control. However, dry weight, plant height and root length showed no significant effects, although those were slightly improved more than those of the control.