• Bulletin of the Korean Chemical Society
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• v.31 no.6
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• pp.1474-1478
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• 2010

An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag ($His_6$-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) ($NTA{\cdot}Ni^{2+}$) on the QD surfaces. We showed that the $NTA{\cdot}Ni^{2+}$-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted $His_6$-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.

• Journal of Institute of Control, Robotics and Systems
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• v.13 no.6
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• pp.560-567
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• 2007

The objective of this research is to optimize the designing parameters of the parallel manipulator with large orientation workspace at the boundary position of the constant orientation workspace (COW). The method uses a simple genetic algorithm(SGA) while considering three different kinematic performance indices: COW and the global conditioning index(GCI) to evaluate the mechanism's dexterity for translational motion of an end-effector, and orientation workspace of two angle of Euler angles to obtain the large rotation angle of an end-effector at the boundary position of COW. Total fifteen cases divided according to the combination of the sphere radius of COW and rotation angle of orientation workspace are studied, and to decide the best model in the total optimized cases, the fuzzy inference system is used for each case's results. An optimized model is selected as a best model, which shows better kinematic performances compared to the basis of the pre-existing model.

• Proceedings of the IEEK Conference
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• 2002.06a
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• pp.275-278
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• 2002

This paper proposes an efficient micro mobility scheme to support user mobility inside an access network which employs Ipv6. The proposed scheme, namely SMM(Sparse-mode Multicast for mobility), utilizes a class of multicast protocol, PIM-SM, in the visited access network to reduce signaling to the home agent as well as to the correspondent node and to minimize packet loss during the handoff when mobile user changes its point of attachment frequently. Through the simulation, the performance of the proposed SMM is analyzed and compared with those of Mobile nv6 and for the case which adpots PIM-DM. The result shows that SMM provides significantly better performance in terms of packet loss that is invariant to transmission rate and packet size.

• Geomechanics and Engineering
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• v.6 no.6
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• pp.545-560
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• 2014

For slope stability analysis, an alternative to the classical limit equilibrium method (LEM) of slices is the shear strength reduction method (SRM), which can be integrated into finite element analysis or finite difference analysis. Recently, probabilistic analysis of earth slopes has been very attractive because it is capable to take the soil uncertainty into account. However, the SRM is less commonly extended to probabilistic framework compared to a variety of probabilistic LEM analysis of earth slopes. To overcome some limitations that hinder the development of probabilistic SRM stability analysis, a new procedure based on recursive algorithm FORM with sensitivity analysis in the space of original variables is proposed. It can be used to deal with correlated non-normal variables subjected to implicit limit state surface. Using the proposed approach, a probabilistic finite element analysis of the stability of an existing earth dam is carried out in this paper.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.83.2-83.2
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• 2019

We study the stability of the magnetic structure in active region (AR) 12371 producing an M6.5 flare on June 22 2015. We first perform a nonlinear force-free fields (NLFFFs) extrapolation to derive three-dimensional (3D) magnetic fields based on time series of observed photospheric magnetic fields. The NLFFFs well describe an observed sigmoidal structure with the shape of a double arc magnetic configuration. Next, we examine three possible instabilities (kink, torus, and double arc) to investigate how the M6.5 flare is triggered in the double arc loops. Consequently, the double arc loops are stable against kink and torus instabilities, but possibly unstable against the double arc instability before the flare occurrence. Finally, we discuss a probable scenario for the M6.5 flare.

• Current Optics and Photonics
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• v.6 no.6
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• pp.634-641
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• 2022

A frequency stable and tunable optoelectronic oscillator (OEO) incorporating an optical phase shifter and a phase-shifted fiber Bragg grating (PS-FBG) is designed and analyzed. The frequency tunability of the OEO can be realized by using a tunable microwave photonic bandpass filter consisting of a PS-FBG, a phase modulator. The optical phase compensation loop is used to compensate for the phase variations of the RF signal from the OEO by adjusting an optical phase shifter. Simulation results demonstrate that the output RF signals of the OEO can be tuned in a frequency range of 118 MHz to 24.092 GHz. When the ambient temperature fluctuates within ±3.9 ℃, the frequency drifts of the output RF signals are less than 68 Hz, the side-mode suppression ratios are more than 69.39 dB, and the phase noise is less than -92.49 dBc/Hz at a 10 kHz offset frequency.

• Journal of Electrical Engineering and Technology
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• v.11 no.6
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• pp.1783-1786
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• 2016

The paper investigates the insulation properties of the supercritical $CO_2$ ($SCCO_2$) fluid as an insulating medium for electrical apparatuses. The insulating material is crucial for electrical apparatuses and $SF_6$ gas has been widely used for high power electrical apparatuses. There have been many research efforts to develop substituents for $SF_6$ gas because of high global warming potential. We obtained above 350 kV/mm insulation strength with 12.0 MPa $SCCO_2$. The positive and negative IEC standard pulses are applied between two 10 mm diameter spherical electrodes. The insulation strength of $SCCO_2$ is at least 2.5 times higher than that of $CO_2$ gas at 6.0MPa. The insulation strength of $SCCO_2$ fluid is about 10 times higher than that of $SF_6$ at 0.5MPa which is the ordinary operating pressure of electrical switchgears. Using the result, we expect that the time for switching and dielectric recovery could be reduced using $SCCO_2$ fluid as an insulating medium.

• Journal of Electrical Engineering and Technology
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• v.11 no.6
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• pp.1846-1856
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• 2016

This study proposes a novel feature point initialization method in order to improve the accuracy of feature point positions by fusing a vision sensor and a lidar. The initialization is a process that determines three dimensional positions of feature points through two dimensional image data, which has a direct influence on performance of a 6-DoF bearing-only SLAM. Prior to the initialization, an extrinsic calibration method which estimates rotational and translational relationships between a vision sensor and lidar using multiple calibration tools was employed, then the feature point initialization method based on the estimated extrinsic calibration parameters was presented. In this process, in order to improve performance of the accuracy of the initialized feature points, an iterative automatic scaling parameter tuning technique was presented. The validity of the proposed feature point initialization method was verified in a 6-DoF bearing-only SLAM framework through an indoor and outdoor tests that compare estimation performance with the previous initialization method.

• Journal of Electrical Engineering and Technology
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• v.6 no.6
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• pp.799-805
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• 2011

This study presents three different magnetization models for identifying unknown magnetization of the ferromagnetic thin plate of a ship. First, the forward problem should be solved to accurately predict outboard magnetic fields due to the magnetization distribution estimated at a certain time. To achieve this, three different modeling methods for representing remanent magnetization (i.e., magnetic charge method, magnetic dipole array method, and magnetic moment method) were utilized. Material sensitivity formulas containing the first-order gradient information of an objective function were then adopted for an efficient search of an optimum magnetization distribution on the hull. The validity of the proposed methods was tested with a scale model ship, and field signals predicted from the three different models were thoroughly investigated with reference to the experimental data.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1571-1576
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• 2016

Alteration of the acetylation status of chromatin and other non-histone proteins by HDAC inhibitors has evolved as an excellent epigenetic strategy in treatment of cancers. The present study was sought to identify compounds with positive pharmacological profiles targeting HDAC1. Analogues of Vorinostat synthesized by Cai et al, 2015 formed the test compounds for the present pharmacological evaluation. Hydroxamte analogue 6H showed superior pharmacological profile in comparison to all the compounds in the analogue dataset owing to its better electrostatic interactions and hydrogen bonding patterns. In order to identify compounds with even better high affinity and pharmacological profile than 6H and Vorinostat, virtual screening was performed. A total of 83 compounds similar to Vorinostat and 154 compounds akin to analogue 6H were retrieved. SCHEMBL15675695 (PubCid: 15739209) and AKOS019005527 (PubCid: 80442147) similar to Vorinostat and 6H, were the best docked compounds among the virtually screened compounds. However, in spite of having good affinity, none of the virtually screened compounds had better affinity than that of 6H. In addition SCHEMBL15675695 was predicted to be a carcinogen while AKOS019005527 is Ames toxic. From, our extensive analysis involving binding affinity analysis, ADMET properties predictions and pharmacophoric mappings, we report Vorinostat hydroxamate analogue 6H to be a potential candidate for HDAC inhibition in treatment of cancers through an epigenetic strategy.