• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.433-438
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• 1991

The plasmid pSY130-14 for the high production of phenylalanine is a temperaturecontrollable expression vector composed of the $P_R$ and the $P_L$ promoter and a temperature sensitive repressor, $cI_{857}$ of bacteriophage lambda. Strain AT2471 harbouring plasmid pSY13O- 14 is induced the phenylalanine production by shifting up the incubation temperaure to $38.5^{\circ}C$. Plasmid stability of E. coli AT2471 harbouring pSY130-14 was very low, it was about 30% after 48 h cultivation at $38.5^{\circ}C$ without kanamycin. The plasmid disappeared immediately at $40^{\circ}C$ without kanamycin, and at $40^{\circ}C$ adding kanamycin, the plasmid stability decreased at the beginning, but rose with the extension of the culture time. For the improvement of plasmid stability, the plasmid obtaind was designated as pSY15O-1 by changing origin region (ori) pACYC 177 of pSY130-14 for ori pSC101. E. coli AT2471 harbouring pSY150-1 was stable at $38.5^{\circ}C$ without tetracycline, and the plasrnid stability was about 40% after 48 h cultivation at $40^{\circ}C$.

• Journal of Korean society of Dental Hygiene
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• v.11 no.2
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• pp.197-211
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• 2011

Objectives : OHIP-14 and EQ-5D were used, targeting the residents of farming communities to identify the elements that influence oral cavity's health and quality of life due to health and to identify the importance of oral cavity's health in order to increase health of adults' oral cavity and quality of life via improved health. Methods : This research was conducted from July 17th, 2010 to August 16th, 2010 targeting 600 residents in Goryeong-gun, Gyeongsangbuk-do, aging over 40. The data has been analyzed using Mann-Whitney U test, Kruskal-Wallis test and hierarchical multiple regression through SPSS Win Program 18.0 version. Results : 1. OHIP-14 and EQ-5D based on general characteristics showed lower oral health-related quality of life and health-related quality of life on the following cases: women (p=0.004, p<0.001), older (p<0.001, p<0.001), lower scholastic ability (p<0.001, p<0.001), lower average of average spending money (p<0.001, p<0.001), higher number of chronic disease (p<0.001, p<0.001), less drinking (p=0.012, p=0.008), lower perceived oral health and health status (p<0.001, p<0.001) and non smoking showed only EQ-5D (p<0.001). 2. OHIP-14 and EQ-5D based on oral health behavior showed lower oral health-related quality of life and health-related quality of life on the following cases: no periodic oral check-up (p<0.001, p<0.001), less experience of oral health education (p<0.001, p<0.001), horizontal tooth-brushing method(p<0.001, p<0.001) and lower frequency of tooth-brushing showed only OHIP-14 (p=0.042). OHIP-14 and EQ-5D based on oral health status and subjective oral symptom showed lower oral health-related quality of life and health-related quality of life on following cases: number of existing tooth less than 20 (p<0.001, p<0.001), the number of missing teeth more than 9 (p<0.001, p=0.044), DMFT (Decay, Missing, Filling Teeth) index more than 18 (p<0.001, p<0.001), wears denture (p<0.001, p<0.001), edentulous (p<0.001, p=0.002), have xerostomia (p<0.001, p<0.001) and have chewing discomfort (p<0.001, p<0.001). 3. Factors affecting OHIP-14 were gender, age, perceived oral health status, perceived health status, number of existing teeth, dental status, xerostomia and chewing discomfort, and the of reliability (how well it explains) the final model was 48.7%. EQ-5D showed relevance on gender, age, presence of chronic disease, perceived health status, xerostomia, chewing discomfort and oral health-related quality of life, and the reliability of the final model was 42.9%. Conclusions : In order to improve the quality of life of ruralists, oral health needs to be improved or remained by increasing the rate of possession of the existing teeth and preventing the loss of teeth. In order to do so, improvement of accessibility of dental clinic, change of direction from treatment-centered to prevention-centered health care system, development of oral health education program and various oral health care policies which would vitalize continuous oral health care system are considered to be necessary.

• Journal of Genetic Medicine
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• v.14 no.1
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• pp.34-37
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• 2017

5p deletion syndrome, also known as Cri-du-Chat syndrome, is a chromosomal abnormality caused by a deletion in the short arm of chromosome 5. Clinical features of 5p deletion syndrome are difficult to identify prenatally by ultrasound examination, thus most cases of 5p deletion syndrome have been diagnosed postnatally. Here, we report eight cases of 5p deletion syndrome diagnosed prenatally, but were unable to find common prenatal ultrasound findings among these cases. However, we found that several cases of 5p deletion syndrome were confirmed prenatally when karyotyping was performed on the basis of abnormal findings in a prenatal ultrasound scan. Hence, it is necessary to carefully perform prenatal ultrasonography for detection of rarer chromosomal abnormalities as well as common aneuploidy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.4
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• pp.465-469
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• 1986

This study was conducted to investigate the proper fertilizer application level and top-dressing method of Lycium chinense MILL in 1984 and 1985. The results obtained were summerized as follows; Stem length was 4cm longer, leaf area was 45-490$\textrm{cm}^2$ larger, dry matter weight per plant was 1.6-5g heavier and average yield of dry fruit of two years was higher as 183kg per l0a in double amount of potassium( 14kg/l0a) application than in standard level of potassium(7kg/l0a). Highly positive correlation was found between absorption amount of inorganic elements of phosporus(l%), nitrogen, potassium(5%) and dry matter weight. Proper fertilizer application level was 13.8kg(N), 14.2kg(P) and 14.3kg(K) per l0a. Dry fruit yield of top-dress-ing plot was 20% heigher than that of basal dressing plot through increase of leaf area per plant and dry matter weight per plant.

• Journal of the East Asian Society of Dietary Life
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• v.14 no.1
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• pp.34-38
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• 2004

This study was carried out to evaluate the effects of Kimchi homogenate product(KHP) on dough characteristics and quality of bread. Breads were prepared by the addition of 0, 7, 14 and 21％ of KHP to the basic formulation. The pH of dough with KHP were ranged from 5.19 to 5.30 and pH of the control was 5.58. Loaf volume index of the bread prepared by adding 7∼21％ KHP increased by 5.4∼17.2％. Color L value of the bread crust and crumb with KHP decreased, color a value of the bread crumb with KHP increased, and b value of the crumb with KHP decreased. The hardness decreased with the addition of KHP, but the cohesiveness and springiness increased with the addition of KHP. Sensory quality of the Kimchi hamburger bread estimated by shape, flavor and overall quality was better than that of the control bread. Especially, the Kimchi hamburger bread quality with addition of 14％ KHP was the best.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.4
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• pp.567-578
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• 2018

In this study, to investigate the effect of the stress reduction of group piles by dynamic loading, a dynamic p-y curve was established and the dynamic p-multiplier was calculated. Dynamic numerical analysis was performed by input sinusoidal waves to the bottom of the pile - ground system for $2{\times}2$ group pile, single pile and $5{\times}5$ group pile, single pile in dry sandy soil, and the pile spacing was changed to 2.5 and 5.0 times of the pile diameter. By establishing and comparing the dynamic p-y curves of the single pile and group piles, the dynamic group pile effect of the piles according to the pile center spacing and row position of the group pile piles is analyzed. $5{\times}5$ showed symmetry of the dynamic P-multiplier value around the pile origin coordinate. The dynamic p-multiplier value at the single pile, $5{\times}5$ pile (pile spacing: 2.5D) is 0.26 ~ 0.30 at the pile number 3, pile number 23, 0.14 pile number 13, and 0.14 ~ 0.38 at the pile number 5, pile number 18. These values differed from the static p-multiplier, especially due to the different loading conditions. The dynamic p-multiplier ($P_{dm}$) estimation through various types of input dynamic loads is expected to be used for dynamic design and analysis of group pile-ground systems of civil foundation structures.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.12
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• pp.6146-6154
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• 2013

This study examined the status on job satisfaction and job performance of Advanced Practice Nurses (APNs) in Korea. The data was obtained from the 2009 APNs' job satisfaction survey of 452 nurses who received the certificate of APN between 2005 and 2008 conducted by the Korean Accreditation Board of Nursing Education. ANOVA and t-test analyses using SPSS version 14.0 were performed. The average job satisfaction level of the APNs was 2.94 out of 5.00. APNs who were performing jobs as a certified APN had a higher level of job satisfaction (t=.23, p<.001), higher wages (t=2.23, p<.05), promotion in rank (t=2.56, p<.05), more professional job performance (t=10.52, p<.001), greatest trust in their bosses and coworkers (t=5.31, p<.001), and higher confidence in their job performance (t=5.07, p<.001) than those who were not. The government and the nursing community need to provide the APNs' with the appropriate supply and demand polices, as well as a proper incentive system and job improvement.

• Animal Bioscience
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• v.35 no.3
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• pp.434-443
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• 2022

Objective: The study was conducted to investigate the impact of boron supplementation on nutrient digestibility, inflammatory responses, blood metabolites and diarrhea index, and their relevance to growth performance in weaned pigs housed in good and poor sanitary environments for 14 days after weaning. Methods: A total of 108 male pigs (Duroc×[Yorkshire×Landrace]) weaned at 21 days of age were used in a randomized complete block design with 2×3 factorial arrangement. Pigs were assigned to three boron treatments (0, 5, and 10 mg/kg) under two environments (good and poor sanitary) to give six replicates per treatment (3 pigs per replicate). On 0, 7, and 14 days, one pig per replicate was euthanized to collect, ileum tissue samples, and rectal fecal samples. Results: Boron supplementation quadratically influenced (p<0.001) feed intake and weight gain in pigs housed in good sanitary conditions from 1 to 14 days post-weaning where pigs offered 5 mg/kg boron optimized weight gain and feed intake. There is a quadratic interaction (p = 0.019) on feed intake for 1 to 14 days post-weaning where 5 mg/kg boron increased feed intake in good sanitary conditions. Pigs housed in the poor sanitary environment decreased (p<0.001) villus height and crypt depth in ileum at days 7 and 14. On day 7 and 14, crude protein digestibility was quadratically influenced (p<0.05) by boron supplementation. Boron supplementation linearly increased (p<0.05) plasma calcium and cholesterol levels whilst linearly (p = 0.005) reducing plasma triglyceride concentrations. Diarrhea index was quadratically influenced (p<0.05) by boron supplementations regardless of sanitary conditions where 5 mg/kg boron inclusion achieved the lowest diarrhea index. Conclusion: Pigs offered 5 mg/kg of boron increased weight gain which may be deduced by improved dry matter, crude protein, and energy digestibility regardless of the sanitary conditions.

• Applied Biological Chemistry
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• v.25 no.3
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• pp.126-134
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• 1982

When $^{14}C-2$, 6-Diethylaniline (2, 6-DEA) was incubated aerobically in soil, $^{14}CO_2$ evolved from non-sterile soil A and B was 6.5 and 10.1%, respectively, in the 21st week. Methanol could extract 3.1 and 13.5% of the radioactivity from soil A and B, respectively, 2, 6-Diethylacetanilide was detected as a degradation product in soil. Chaetomium globosum produced 2, 6-diethyl-p-benzoquinone as a degradation product in pure culture. A possible pathway was proposed to include p-hydroxylation of 2, 6-DEA, formation of quinoneimine, and the subsequent hydrolysis with the release of ammonia.

• Korean Journal of Food Science and Technology
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• v.22 no.2
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• pp.177-182
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• 1990

The changes in the partition coefficient of model proteins (lysozyme, myoglobin, conalbumin, bovine serum albumin) in an aqueous two·phase system formed by polyethylene glycol and dextran were examined in order to improve the capacity of counter current distribution (CCD) for the protein fractionation and concentration . The protein distribution pattern in CCD with 30 tubes varied with the pH (4.5, 5.5, 6.5, 9.0, 12.0) and KCl concentration (0mM, 50mM, 250mM, 500mM) of the system. From the mixture of model proteins, pure myoglobin was appeared at the upperphase of 14th tube having 50mM of KCl at pH 5.5 and the upper-phase of 13th tube having 250mM of KCl at pH 6.5. Similarly pure BSA was obtained at the 14th tube having KCl 250mM with pH 4.5, pure lysozyme at the 19th tube having 500mM of KCl at pH 4.5 and the upper-phase of 16th tube 50mM of KCl at pH 5.5.