• Title/Summary/Keyword: 5hmC

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The Effect of HCl Gas on Selective Catalytic Reduction of Nitrogen Oxide (질소산화물의 선택적 환원 제거시 염화수소기체가 촉매에 미치는 영향)

  • Choung, Jin-Woo;Choi, Kwang-Ho;Seong, Hee-Je;Chai, Ho-Jung;Nam, In-Sik
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.4
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    • pp.609-617
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    • 2000
  • This study is aimed at investigating an effect of HCl gas on selective reduction of NOx over a CuHM and $V_2O_5-WO_3/TiO_2$ catalyst. SCR process is the most effective method to remove NOx, but catalyst can be deactivated by the acidic gas such as HCl gas which is also included in flue gas from the incinerator. In dry condition of flue gas, the CuHM catalyst treated by HCl gas has shown higher NO removal activity than the fresh catalyst. The activity of the catalyst can be restored by treating at $500^{\circ}C$. On the contrary. $V_2O_5-WO_3/TiO_2$ catalyst is obviously deactivated by HCl and the deactivation increases in proportion to the concentration of HCl gas. The deactivated catalyst is not restored to it's original activity by heat treatment for regeneration. In wet flue gas stream, the CuHM catalyst has shown lower activity than fresh catalyst and $V_2O_5-WO_3/TiO_2$ catalyst was severely deactivated by HCl treatment. The activity loss of catalysts are mainly due to the decrease of Br$\ddot{o}$nsted acid site on the catalyst surface by $NH_3$ TPD. The change of BET surface area of CuHM catalyst after the reaction isn't observed but $V_2O_5-WO_3/TiO_2$ catalyst is observed. The amount of $Cu^{{+}{+}}$ and $V_2O_5$ is decreased after the reaction. From these results, it is expected that CuHM catalyst should be better than $V_2O_5-WO_3/TiO_2$ catalyst for its application to the incineration of flue gas.

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Development of Cryptosporidium parvum in cell culture (세포배양에서 Cryptosporidium parvum의 발육)

  • Kim, Bo-sook;Joo, Hoo-don;Wee, Sung-hwan;Kim, Tae-jong
    • Korean Journal of Veterinary Research
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    • v.35 no.2
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    • pp.317-326
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    • 1995
  • The purpose of this study was to establish a method for in vitro culture of C parvum isolated in Korea by determination of suitable cell model to complete development of this parasite. The result obtained were summerized as follows: 1. To determine the most suitable cell line, six types of cell line were examined by microscopy. All cell lines were infected with C parvum and showed the highest infection score in HmLu cells. 2. The staining methods including DMSO-modified acid-fast(A-F) stain, hematoxylin-eosin(H & E) stain and immunofluorescence antibody(IFA) stain were applied to examine the infection of C parvum in cell culture. These staining methods were possible to examine the infection of C parvum in cell culture. The most sensitive one was IFA staining technique. 3. Developmental stages of C parvum in HmLu cell were observed. After the initial 8 hour incubation period, some trophozoites were observed. The meronts and gametes were appeared at 24-48 hour post inoculation(PI), and oocysts were observed firstly at 48-72 hour PI. 4. In H & E stain, the parasite appeared as basophilic within parasitophorous vacuole membrane(PVM) and lying in cytoplasm at near the nucleus of the host cells. It was able to distinguish the type I, type II meronts and gametes. 5. In DMSO-modified acid-fast stain, specific stained parasites were appeared firstly after 48 hour PI. The parasites were showed with different degrees of staining bright red color within PVM. 6. The endogenous stages of parasites in HmLu cell recovered at 48, 96, 120 and 144 hour after inoculation were reacted with rabbit immunized serum in immunofluorescence antibody and avidin-biotin complex peroxidase staining technique.

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Shear-induced structure and dynamics of hydrophobically modified hydroxy ethyl cellulose (hmHEC) in the presence of SDS

  • Tirtaatmadija, Viyada;Cooper-white, Justin J.;Gason, Samuel J.
    • Korea-Australia Rheology Journal
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    • v.14 no.4
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    • pp.189-201
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    • 2002
  • The interaction between hydrophobically modified hydroxyethyl cellulose (hmHEC), containing approximately 1 wt% side-alkyl chains of $C_{16}$, and an anionic sodium dodecyl sulphate (SDS) surfactant was investigated. For a semi-dilute solution of 0.5 wt% hmHEC, the previously observed behaviour of a maximum in solution viscosity at intermediate SDS concentrations, followed by a drop at higher SDS concentrations, until above the cmc of surfactant when the solution resembles that of the unsubstituted polymer, was confirmed. Additionally, a two-phase region containing a hydrogel phase and a water-like supernatant was found at low SDS concentrations up to 0.2 wt%, a concentration which is akin to the critical association concentration, cac, of SDS in the presence of hmHEC. Above this concentration, SDS molecules bind strongly to form mixed micellar aggregates with the polymer alkyl side-chains, thus strengthening the network junctions, resulting in the observed increase in viscosity and elastic modulus of the solution. The shear behaviour of this polymer-surfactant complex during steady and step stress experiments was examined In great detail. Between SDS concentrations of 0.2 and 0.25 wt%, the shear viscosity of the hmHEC-polymer complex network undergoes shear-induced thickening, followed by a two-stage shear-induced fracture or break-up of the network. The thickening is thought to be due to structural rearrangement, causing the network of flexible polymers to expand, enabling some polymer hydrophobic groups to be converted from intra- to inter-chain associations. At higher applied stress, a partial local break-up of the network occurs, while at even higher stress, above the critical or network yield stress, a complete fracture of the network into small microgel-like units, Is believed to occur. This second network rupture is progressive with time of shear and no steady state in viscosity was observed even after 300 s. The structure which was reformed after the cessation of shear is found to be significantly different from the original state.

Changes in Color Intensity of Extract from Hololeion Maximowiczii Root by Drying Methods (건조방법에 따른 께묵뿌리 추출물의 색도변화)

  • Choi, Dong-Yeon;Do, Jae-Ho;Lee, Kwang-Seung;Yang, Cha-Bum
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.417-420
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    • 1993
  • Changes in color intensity of Hololeion maximowiczii(HM) roots which were treated with freeze drying (FD), $50^{\circ}C$ hot air drying (HAD) and $50^{\circ}C$ hot air drying after steam blanching (HADB) were investigated in this study. Color of HADB was yellow-green while other treatments showed bright brown color. In brown color intensity, optical density at 420 nm of FD treatment was highest but HADB treatment showed the highest value in ultra violet spectrum. Water soluble pigment of HM in state of aquous solution was more unstable for heat treatment than that in state of powder. Activation energy for decomposition of the pigment was 20.9 kcal/mole between $90{\sim}100^{\circ}C$ but 50.6 kcal/mole between $80{\sim}90^{\circ}C$.

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Structure of Antennal Sensilla on the Adult Asian Ladybird, Hamonia axyridis Pallas (Coleoptera: Coccinellidae) (무당벌레(Harmonia axyridis)성충 더듬이에 분포하는 감각기의 구조)

  • Park, Soo-Jin;Oh, Hyun-Woo;Youn, Young-Nam;Park, Ho-Yong
    • Applied Microscopy
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    • v.31 no.1
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    • pp.91-99
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    • 2001
  • The antennal sensilla of the adult male and female ladybird beetle, Harmonia axyridis (Coleoptera: Coccinellidae) were examined by scanning electron microscopy. Nine types of sensilla were characterized and grouped into 5 morphological classes: $B\ddot{o}hm$, basiconic, chetiform, coeloconlc, and trichoid sensilla. $B\ddot{o}hm$ sensilla were localized on the scape and pedicel, part of the ventral and dorsal proximal side. This placement suggests a role in proportion of antennal position and movement. Basiconic and chetiform sensilla were divided into 3 subtypes by morphology and length differences. Trichoid sensilla are confined to the last 2 flagellum segments on both sexes. Sexual dimorphism of antennal sensilla is limited to the three types of difference. The principal characteristics of this dimorphism are the following: a) The distribution of sensilla was differ from male and female in antennae. b) Males exhibit coeloconic sensilla not present in females; c) Females posses more trichoid sensilla than males. The possible role of specific dilference of the sensilla in intersexual communication is discussed.

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Cloning and Nucleotide Sequence Analysis of xylC Gene Encoding 5C-2HMS Dehydrogenase from Pseudomonas sp. S-47. (Pseudomonas sp. S-47로부터 5-Chloro-2-Hydroxymuconic Semialdehyde Dehydrogenase를 암호화하는 xylG 유전자의 클로닝 및 염기서열 분석)

  • Park, Song-Yi;Lee, Dong-Hoon;Kim, Young-Soo;Lee, Kyung;Kim, Chi-Kyung
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.8-14
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    • 2002
  • Pseudomonas sp. S-47 is capable of degrading 4-chlorobenzoate to produce 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by the enzymes encoding by xylXYZLTE cluster. In this study, the resulting 5C-2HMS was confirmed to be transformed to 5-chloro-2-hydroxymuconic acid (5C-2HMA) by 5C-2HMS dehydrogenase. The xylG gene encoding 5C-2HMS dehydrogenase was cloned from the chromosomal DNA of strain S-47. The nucleotide sequence of xylG showed to be composed of 1,600 base pairs with ATG initiation and TGA termination codons. A deduced amino acid sequence of the 5C-2HMS dehydrogenase (XylG) exhibited 98%, 93%, and 89% identity with those of the dehydrogenases from P. putida mt-2, P. putida G7, and Pseudomonas sp. CF600, respectively.

Effect of prenatal different auditory environment on learning ability and fearfulness in chicks

  • Zhao, Shuai;Xu, Chunzhu;Zhang, Runxiang;Li, Xiang;Li, Jianhong;Bao, Jun
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1454-1460
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    • 2022
  • Objective: Early environmental enrichment in life can improve cognition in animals. The effect of prenatal auditory stimulation on learning ability and fear level in chick embryos remained unexplored. Therefore, this study investigated the effect of prenatal auditory stimulation on the learning ability and fear level of chicks. Methods: A total of 450 fertilized eggs were randomly divided into 5 groups, including control group (C), low-sound intensity music group (LM), low-sound intensity noise group (LN), high-sound intensity noise group (HN) and high-sound intensity music group (HM). From the 10th day of embryonic development until hatching, group LM and group LN received 65 to 75 dB of music and noise stimulation. Group HN and group HM received 85 to 95 dB of noise and music stimulation, and group C received no additional sound. At the end of incubation, the one-trial passive avoidance learning (PAL) task and tonic immobility (TI) tests were carried out, and the serum corticosterone (CORT) and serotonin (5-HT) concentrations were determined. Results: The results showed that compared with the group C, 65 to 75 dB of music and noise stimulation did not affect the PAL avoidance rate (p>0.05), duration of TI (p>0.05) and the concentration of CORT (p>0.05) and 5-HT (p>0.05) in chicks. However, 85 to 95 dB of music and noise stimulation could reduce duration of TI (p<0.05) and the concentration of CORT (p<0.05), but no significant effect was observed on the concentration of 5-HT (p>0.05) and PAL avoidance rate (p>0.05). Conclusion: Therefore, the prenatal auditory stimulation of 85 to 95 dB can effectively reduce the fear level of chicks while it does not affect the learning ability.

Production of Foamed Glass by Using Hydrolysis of Waste Glass(III) - Heat Treatment for Stabilization and Scale-up Test - (폐유리의 가수분해 반응에 의한 발포유리의 제조(III) - 안정화 열처리공정 및 Scale-up Test -)

  • Lee, Chul-Tae;Um, Eui-Heum
    • Applied Chemistry for Engineering
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    • v.17 no.1
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    • pp.73-81
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    • 2006
  • Heat treatment condition for the stabilization of foamed glass block through the foaming process of the hydrolized waste glass was investigated and scale-up test for the manufacturing of foamed glass was also attempted for the actual foaming process. Proper heat treatment condition was quenching from the foaming temperature to $550{\sim}600^{\circ}C$ for stabilization, and then annealing from stabilization temperature to $200^{\circ}C$ and holding up at $200^{\circ}C$ for removal thermal stress, and then annealing to the room temperature with cooling speed of $0.3^{\circ}C/min$. Through this heat treatment conditions, foamed glass block with size of $250mm{\times}250mm{\times}90mm$ was produced successfully. The properties of this foamed glass block showed density of $0.28{\pm}0.06g/cm^3$, thermal conductivity of $0.048{\pm}0.005kcal/hm^{\circ}C$, moisture absorption of $0.5{\pm}0.09vol%$, linear expansion coefficient of $(8.6{\pm}0.2){\times}10^{-6}m/m^{\circ}C$($400^{\circ}C$), flexural strength of $15.0{\pm}0.6kg/cm^2$, and compression strength of $39.5{\pm}0.6kg/cm^2$.

AN EVALUATION OF MECHANICAL PROPERTIES OF LIGHT-CURED RESTORATIVE COMPOSITES BY INDENTATION AND COMPRESSION TEST (압입과 압축에 의한 광중합형 수복용 복합레진의 기계적 성질 평가)

  • Lee, Yong-Woo;Yu, Mi-Kyung;Cho, Young-Gon;Bae, Tae-Sung;Lee, Kwang-Won
    • Restorative Dentistry and Endodontics
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    • v.24 no.3
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    • pp.511-518
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    • 1999
  • This study was performed to evaluate the mechanical properties of light-cured restorative composites by compression and indentation tests. Five commercially available light-activated composites (Heliomolar : HM, Aelitefil : AF, Amelogen : AG, Clearfil AP-X : CF, Z100 ZH) were used Disc specimens of 12mm in diameter and 1 mm in thickness and Cylindrical specimens of 3mm in diameter and 6mm in length were prepared for the indentation and compressive test, respectively. All specimens were immersed in distilled water at $37^{\circ}C$ for 30 days. An indentation test of 68-degree trigonal diamond pyramid was made under 10 g load for 15 seconds and an Knoop indentation test was made under 50 g load for 15 seconds. Hardness numbers, characteristic indentation depths and permanent deformation were measured during indentation of the 68-degree trigonal diamond pyramid compressive test was carried out at a crosshead speed of 0.5mm/min. The results obtained were summarized as follows, 1. The highest hardness value was obtained in the CF group and the lowest value was obtained in the HM group. Hardness values showed no significant, difference between AG group and HM group but other groups showed the significant differences in each group(p<0.05). 2. Hardness number by Knoop pyramid were higher than those of 68-degree trigonal diamond pyramid. 3. Plastic deformation during the indentation of 68-degree trigonal diamond pyramid was the lowest in the CF group and the largest in the HM group. Results of Tukey test showed the significant difference between CF group and others; also between ZH and AF groups and AG and HM groups(p<0.05) 4. The highest compressive strength was obtained in the CF group and the lowest compressive strength was obtained in the ZH group. Compressive strength values showed no significant difference between CF group and ZH group but other groups showed the significant differences in each group(p<0.05).

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Transmethylation of Methylnaphthalene Over Beta, USY and Mordenite Zeolite Catalysts (제올라이트 Beta, USY, Mordenite 촉매를 이용한 Methylnaphthalene의 트랜스메틸화 반응)

  • Park, Jung-Nam;Park, Yong-Ki;Baeg, Jin-Ook;Hong, Suk-In;Lee, Chul Wee
    • Korean Chemical Engineering Research
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    • v.43 no.5
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    • pp.560-565
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    • 2005
  • Catalytic performances of zeolite catalysts such as H-mordenite(HM), H-Beta$(H{\beta})$, H-USY(HUSY) for the transmethylation between 1-methylnaphthalene(1-MN) and 2-methylnaphthalene(2-MN) were investigated in a fixed-bed flow reactor. $H{\beta}$ showed higher and more stable conversion than others to exhibit a high and stable 2-MN/1-MN ratio of 2.3 and 2,6-DMN/2,7-DMN ratio of 1.3 at the $1^{st}$ hour of time on stream under the reaction conditions as follows: reaction temperature of $350^{\circ}C$, reaction pressure of 1.5 MPa, WHSV of $2.7g_{feed}/g_{cat}{\cdot}h$ and the molar ratio of 1-MN and 2-MN of 1:1. The catalytic behavior has been discussed in relation with the catalyst pore structure and acidity.