• Title/Summary/Keyword: 5S rDNA

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A New Species of Hyphomycetes, Aspergillus coreanus sp.nov.,Isolated from Traditional Korean Nuruk

  • Yu, Tae-Shick;Yeo, Soo-Hwan;Kim, Hyun-Soo
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.182-187
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    • 2004
  • Strain NR $15-1^T$ isolated from traditional Korean Nuruk is described as a new species and named as Aspergillus coreanus NR $15-1^T$ sp. novo Strain NR $15-1^T$ grew rapidly to form yellow-green colonies whose surfaces were velvety on Czapek solution agar. Conidial heads were yellow to light and elliptical, whereas the conidiophore was colorless and typically long. In addition, vesicles were from flask-shaped to globose, and sterigmata are uniseriate. Conidia were spherical and deep yellow-green, and their surfaces were lightly roughened. The G+C content of strain NR $15-1^T$ was 51 mol% and strain NR $15-1^T$contained a dihydrogenated ubiquinone with Q9 (94.9%) as a major quinone. The nucleotide sequences of strain NR $15-1^T$ in the two Internal Transcribed Spacers (ITS 1 and 2) and 5.8S rDNA showed highest similarity when compared with that of A. tubingensis and A. phoenicis NRRL $365^T$. However, based on morphological and chemotaxonomic characteristics, this strain was different from A. tubingensis and A. phoenicis NRRL $365^T$. On the basis of the data presented, it is proposed that strain NR $15-1^T$ should be placed in the genus Aspergillus as a new species, Aspergillus coreanus sp. novo Therefore, the type strain of the new species is strain NR $15-1^T$ (=KCTC 18075P^T,=KCCM 80006^T$.

Isolation and Identification of Streptomyces sp. Producing Anti-vancomycin Resistant Staphylococcus aureus Substance (반코마이신 내성 Staphylococcus aureus 억제 물질 생산 Streptomyces sp.의 분리 및 동정)

  • Oh Se-Teak;Lee Jun-Jae;Lee Ji-Youn;Kim Jin-Kyu;Yang Si-Yong;Kim Yang-Soo;Song Min-Dong
    • Microbiology and Biotechnology Letters
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    • v.33 no.2
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    • pp.90-95
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    • 2005
  • An Actinomycetes producing an anti-VRSA (vancomycin-resistant Staphylococcus aureus) substance was isolated from soil. The cultural, morphological, physiological and phylogenetic analyses of an isolated strain were investigated for identification. Cultural characteristics based on ISP (International Streptomyces Project) were as follows: white aerial mycelium, yellow reverse side, and good growth on various medium. Also, the isolate did not produce the soluble pigment. Morphological characteristics were showed cylindrical spore chain and smooth spore surface by SEM (Scanning Electron Microscope). Physiological characteristics were showed LL-type by DAP isomer analysis and detected glycine, glutamic acid and alanine. A phylogenetic analysis of the 16S rDNA provided a clue that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces echinatus. The isolate was identified to be a genus of Streptomyces sp.. The optimal culture conditions for the maximum production of anti-VRSA substance by Streptomyces sp. were attained in a culture medium composed of $2.0\%$ (w/v) glucose, and $0.4\%$ (w/v) yeast extract. The anti-VRSA substance was highly produced after 5 days of culture. Optimal pH and temperature conditions for the production of anti-VRSA substance were pH 7.0 and $28^{\circ}C$, respectively.

First Morphological Description and the Distribution of Ophisurus macrorhynchos (Anguilliformes: Ophichthidae) Leptocephalus Collected from Southeastern Waters of Jeju Island (제주도 남동부해역에서 채집된 바다뱀(Ophisurus macrorhynchos) (뱀장어목: 바다뱀과) 엽상자어의 첫 형태기재 및 분포특성)

  • Ji, Hwan-Sung;Choi, Jung Hwa;Choi, Kwang Ho;Yoon, Sang Chul;Lee, Dong Woo;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.6
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    • pp.888-894
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    • 2014
  • Seventeen specimens of leptocephali [9.8-44.5 mm total length (TL)], of the family Ophichthidae, were collected from southeastern waters off Jeju Island and the Korea-Japan intermediate zone, and identified by means of morphology and genetics. These specimens were identified as belonging to the subfamily Ophichthinae based on various combinations of morphological characters: 211-215 total myomeres; 7 gut swellings; 2 liver lobes connected with the gall bladder on the second lobe; 6-7 postanal pigments present from anus to caudal margin. An analysis of the partial 12S rRNA sequences (849 base pairs) of mitochondrial DNA showed that our specimens must be Ophisurus macrorhynchos because their sequences were concordant with those of the adult O. macrorhynchos (genetic distance = 0.000). Furthermore, their total myomeres were consistent with those of the O. macrorhynchos adult. This is the first time that the morphological characteristics of O. macrorhynchos leptocephali have been described for Korean waters, and we suggest diagnostic characteristics for the genus Ophisurus leptocephali. We hypothesize that one of the spawning grounds of O. macrorhynchos is located in the southeastern part of Jeju Island.

Occurrence of Sweet-potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) and Its Response to Insecticide in Gyeonggi Area (경기지역에서 담배가루이의 발생 및 약제반응)

  • Lee, Young-Su;Kim, Jin-Young;Hong, Soon-Sung;Park, Jungan;Park, Hong-Hyun
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.377-382
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    • 2012
  • This study investigated the occurrence of sweet potato whitefly, Bemisia tabaci affecting cucumber, eggplant and red pepper, as well as sweet potato species, and its response to insecticides in Gyeonggi province from 2010 to 2011. Sweet potato whitefly is widespread throughout the southern part of Gyeonggi province. Most regional populations of B. tabaci belong to biotype Q having been reported in the south Korea since 2005, but in Goyang mixed populations of two biotypes (B and Q) were found. Survey results of Tomato Yellow Leaf Curl Virus (TYLCV) disease that was vectored by B. tabaci indicated that this virus disease was not spread throughout the Gyeonggi province. Biotype Q of B. tabaci was found to be resistant to neonicotinoid insecticides, whereas biotype B was highly susceptible to them.

Purification and Characterization of a Protease Produced by a Planomicrobium sp. L-2 from Gut of Octopus vulgaris

  • Liu, Qing;Sun, Shujing;Piao, Meizi;Yang, Ji Young
    • Preventive Nutrition and Food Science
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    • v.18 no.4
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    • pp.273-279
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    • 2013
  • Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease was obtained from the strain Planomicrobium sp. L-2 through ammonium sulfate precipitation, dialysis and enrichment, DEAE-Sephadex A50 anion-exchange chromatography, and Sephadex G-100 gel chromatography. The molecular weight and properties of the protease were characterized, including optimum temperature and pH, thermal stability, protease inhibitions and metal ions. According to our results, the protease from Planomicrobium sp. L-2 strain designated as F1-1 was obtained by three-step separation and purification from crude enzyme. The molecular weight of the protease was 61.4 kDa and its optimum temperature was $40^{\circ}C$. The protease F1-1 showed a broad pH profile for casein hydrolysis between 5.0~11.0. No residual activity was observed after incubation for 40 min at $60^{\circ}C$ and 60 min at $50^{\circ}C$. F1-1 protease was inhibited by $Mn^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ ions, as well as PMSF, indicating that the protease F1-1 was a serine protease. Additionally, research basis provided by this study could be considered for industrial application of octopus intestinal proteases.

Xanthomonas euvesicatoria Causes Bacterial Spot Disease on Pepper Plant in Korea

  • Kyeon, Min-Seong;Son, Soo-Hyeong;Noh, Young-Hee;Kim, Yong-Eon;Lee, Hyok-In;Cha, Jae-Soon
    • The Plant Pathology Journal
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    • v.32 no.5
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    • pp.431-440
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    • 2016
  • In 2004, bacterial spot-causing xanthomonads (BSX) were reclassified into 4 species-Xanthomonas euvesicatoria, X. vesicatoria, X. perforans, and X. gardneri. Bacterial spot disease on pepper plant in Korea is known to be caused by both X. axonopodis pv. vesicatoria and X. vesicatoria. Here, we reidentified the pathogen causing bacterial spots on pepper plant based on the new classification. Accordingly, 72 pathogenic isolates were obtained from the lesions on pepper plants at 42 different locations. All isolates were negative for pectolytic activity. Five isolates were positive for amylolytic activity. All of the Korean pepper isolates had a 32 kDa-protein unique to X. euvesicatoria and had the same band pattern of the rpoB gene as that of X. euvesicatoria and X. perforans as indicated by PCR-restriction fragment length polymorphism analysis. A phylogenetic tree of 16S rDNA sequences showed that all of the Korean pepper plant isolates fit into the same group as did all the reference strains of X. euvesicatoria and X. perforans. A phylogenetic tree of the nucleotide sequences of 3 housekeeping genes-gapA, gyrB, and lepA showed that all of the Korean pepper plant isolates fit into the same group as did all of the references strains of X. euvesicatoria. Based on the phenotypic and genotypic characteristics, we identified the pathogen as X. euvesicatoria. Neither X. vesicatoria, the known pathogen of pepper bacterial spot, nor X. perforans, the known pathogen of tomato plant, was isolated. Thus, we suggest that the pathogen causing bacterial spot disease of pepper plants in Korea is X. euvesicatoria.

Mineral Phosphate Solubilization by Wild Type and Radiation Induced Mutants of Pantoea dispersa and Pantoea terrae

  • Murugesan, Senthilkumar;Lee, Young-Keun;Kim, Jung Hun
    • Journal of Radiation Industry
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    • v.3 no.1
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    • pp.39-45
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    • 2009
  • Three mineral phosphate solubilizing (MPS) bacteria where isolated from rhizosphere soil samples of common bean and weed plants. 16S rDNA analysis indicated that the isolate P2 and P3 are closely related to Pantoea dispersa while isolate P4 is closely related to Pantoea terrae. Isolates P2 and P3 recorded $381.60{\mu}g\;ml^{-1}$ and $356.27{\mu}g\;ml^{-1}$ of tricalcium phosphate (TCP) solubilization respectively on 3 days incubation. Isolate P4 recorded the TCP solubilization of $215.85{\mu}g\;ml^{-1}$ and the pH was dropped to 4.44 on 24 h incubation. Further incubation of P4 sharply decreased the available phosphorous to $28.94{\mu}g\;ml^{-1}$ and pH level was raised to 6.32. Gamma radiation induced mutagenesis was carried out at $LD_{99}$ dose of the wild type strains. The total of 14 mutant clones with enhanced MPS activity and 4 clones with decreased activity were selected based on solubilization index (SI) and phosphate solubilization assay. Mutant P2-M1 recorded the highest P-solubilizing potential among any other wild or mutant clones by releasing $504.21{\mu}g\;ml^{-1}$ of phosphorous i.e. 35% higher than its wild type by the end of day 5. A comparative evaluation of TCP solubilization by wild type isolates of Pantoea and their mutants, led to select three MPS mutant clones such as P2-M1, P3-M2 and P3-M4 with a potential to release >$471.67{\mu}g\;ml^{-1}$ of phosphorous from TCP. These over expressing mutant clones are considered as suitable candidates for biofertilization.

Effect of Green Manure Crops Incorporation for Reduction of Pythium zingiberum in Ginger Continuous Cultivation (생강연작재배지에서 Pythium zingiberum 경감을 위한 녹비작물 재배효과)

  • Jung, Yu Jin;Nou, III Sup;Kim, Yong Kwon;Kang, Kwon Kyoo
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.271-278
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    • 2015
  • This experiment was carried out to evaluate the effects of green manure crops on the changes of chemical properties and microorganisms in soil of continuous ginger cultivation. The biomass and nitrogen absorption of green manure crop were the highest in crimson clover. After cropping, soil phosphate content was the lowest in orchard grass, however, T-N content was the highest of hairy vetch > crimson clover > orchard grass. Also real-time PCR analysis was conducted to measure density of Pythium zingiberum in soil of before and after incorporation of green manure crop. Density of P. zingiberum was the lowest of all the green manures. In this results are summarized that green manure cropping reduced salt accumulation and density of P. zingiberum in continuous ginger cultivation.

Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran (쌀겨와 밀기울의 토양 혐기발효 처리가 시설 재배지 토양의 미생물상에 미치는 영향)

  • Kim, Hong-Lim;Weon, Hang-Yeon;Sohn, Bo-Kyun;Choi, Young-Hah;Kwack, Young-Bum
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.341-347
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    • 2009
  • Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20 days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.

Characteristics of Potential Gamma-Aminobutyric Acid-Producing Bacteria Isolated from Korean and Vietnamese Fermented Fish Products

  • Vo, Thi Thu-Thao;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.29 no.2
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    • pp.209-221
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    • 2019
  • Gamma-aminobutyric acid (GABA) is a neurotransmitter that exerts several physiological functions and positive effects on human health. The aim of this study was to isolate and characterize the strains that had GABA-producing abilities from various fermented fish products. A total of 91 acid-producing strains were isolated from 41 samples of fermented fish products, and 27 strains showing GABA-producing abilities were identified by the 16S rDNA sequences. Among the strains, 31% strains tolerated at high-salt environment of 10-20% throughout the fermentation of fish sauces. The 27 isolates that produced GABA at various concentrations did so in the range of 5 to 454 mM. These GABA-producing isolates were identified as lactic acid bacteria of 14 strains, which included twelve Lactococcus lactis, one Enterococcus faecium, and one Lactococcus pentosus; eight Bacillus cereus group, which included seven B. thuringiensis and one B. cereus; and five Staphylococcus spp. Interestingly, with Vietnamese fish sauces, we mostly identified species of B. thuringiensis and Staphylococcus spp., while with Korean fermented fish products, the majority of the strains identified belonged to L. lactis. Among the strains, B. thuringiensis LH2134 produced the highest levels of GABA at 366 mM among the strains identified from Vietnamese fish sauces, whereas L. lactis LA43, a new strain isolated from Korean jeotgal (salted shrimp paste), produced the highest amount of GABA at 454 mM and the glutamate concentration in the medium was essential for GABA accumulation. Therefore, such the isolates might serve as good starters for development of more GABA-reinforced foods among fermented fish products.