• BMB Reports
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• v.35 no.3
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• pp.283-290
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• 2002

The glycogen-associated protein phosphatase (PP1G/$R_{GL}$) may play a central role in the hormonal control of glycogen metabolism in the skeletal muscle. Here, we investigated the in vivo epinephrine effect of glycogen metabolism in the skeletal muscle of the wild-type and $R_{GL}$ knockout mice. The administration of epinephrine increased blood glucose levels from 200±20 to 325±20 mg/dl in both wild-type and knockout mice. Epinephrine decreased the glycogen synthase -/+ G6P ratio from 0.24±0.04 to 0.10±0.02 in the wild-type, and from 0.17±0.02 to 0.06±0.01 in the knockout mice. Conversely, the glycogen phosphorylase activity ratio increased from 0.21±0.04 to 0.65±0.07 and from 0.30±0.04 to 0.81±0.06 in the epinephrine trated wild-type and knockout mice respectively. The glycogen content of the knockout mice was substantially lower (27%) than that of both wild-type mice; and epinephrine decreased glycogen content in the wild-type and knockout mice. Also, in Western blot analysis there was no compensation of the other glycogen targeting components PTG/R5 and R6 in the knockout mice compared with the wild-type. Therefore, $R_{GL}$ is not required for the epinephrine stimulation of glycogen metabolism, and rather another phosphatase and/or regulatory subunit appears to be involved.

• Natural Product Sciences
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• v.8 no.2
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• pp.35-43
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• 2002

Glycosides of herbal medicines, such as glycyrrhizin, ginsenosides, kalopanaxsaponins, rutin and ponicirin, were studied regarding their metabolic fates and pharmacological actions in relation to intestinal bacteria using germ-free, gnotobiotic and conventional animals. When glycyrrhizin (GL) was orally administered, $18{\beta}-glycyrrhetinic\;acid\;(GA)$, not GL, was detected in plasma and intestinal contents of gnotobiotic and conventional rats. However, GA could not be detected in germ-free rats. When GL was incubated with human intestinal bacteria, it was directly metabolized to GA (>95%) or via $18{\beta}-glycyrrhetinic\;acid-3-{\beta}-D-glucuronide$(>5%). Orally administered GL was effective in gnotobiotic and conventional rats for liver injury induced by carbon tetrachloride, but was not effective in germ-free rats. When ginseng saponins were orally administered to human beings, compound K in the plasma was detected, but the other protopanxadiol saponins were not detected. The compound K was active for tumor metastasis and allergy. When kalopanaxsaponins were incubated with human intestinal microflora, they were metabolized to kalopanaxsaponin A, kalopanaxsaponin I and hederagenin. These metabolites were active for rheumatoid arthritis and diabetic mellitus while the other kalopanxsaponins were not. When flavonoid glycosides were orally administered to animals, aglycones and/or phenolic acids were detected in the urine. The metabolic pathways proceeded by intestinal bacteria rather than by liver or blood enzymes. These metabolites, aglycones and phenolic acids, showed antitumor, antiinflammatory and antiplatelet aggregation activities. These findings suggest that glycosides of herbal medicines are prodrugs.

• Proceedings of the Korea Information Processing Society Conference
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• 2015.10a
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• pp.1632-1635
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• 2015

발전하는 IT 기술은 사회, 문화, 교육 등 전 산업 분야에 많은 영향을 미치고 있으며, e-book 및 콘텐츠 시장은 여러 산업분야에서 주목받고 있는 중요한 기술개발분야 중에 한 부분이라고 할 수 있다. 하지만 현재까지 e-book의 경우 비 표준화 확장, 단 방향성 비 상호작용, 2차원적인 콘텐츠 사용 등 여러가지 한계적 사용에 문제점을 가지고 있었다. 따라서 본 논문에서는 WebGL 3D 콘텐츠를 이용한 인터렉티브 가상 시뮬레이션 e-book 협업 저작도구를 설계하고 구현하였다. 본 연구에서는 표준화된 HTML5 기술과 IDPF에서 제안된 EPUB3를 통해 기존의 e-book 활용의 한계점들을 극복하고, WebGL 웹 표준 기술을 적용하여 인터렉티브 가상 시뮬레이션 사용이 가능한 전자책 그래픽 요소를 구현 하였다. 향후 본 연구를 통해 표준화된 e-book 콘텐츠 시장의 활성화와 효율적인 교육 도구의 발전을 기대한다.

• Journal of the Korean Wood Science and Technology
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• v.44 no.4
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• pp.477-493
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• 2016

A single process using hot water (0% green liquor) and green liquor (GL) was investigated for pre-pulping extraction on two types of raw material. The GL was applied at different alkali charges of 0-5% on a dry wood weight basis. The extractions were performed at an H-factor 900 at $180^{\circ}C$. The 0% and 3% GL extraction detected acetic acid (AA) at 10.02 and $9.94g/{\ell}$, extracts derived from hardwood, 2.46 and $3.76g/{\ell}$, extracts derived from softwood, respectively. The single liquid-liquid extraction (LLE) was studied using tri-n-alkylphosphine oxide (TAPO). Response surface methodology (RSM) was employed as an efficient approach for predictive model building and optimization of AA recovery conditions. The extraction of AA was evaluated with a three-level factorial design. Three independent variables, pH (0.5-3.5), temperature ($25-65^{\circ}C$), and residence time (24-48 min) were investigated. Applying the RSM models obtained, the optimal conditions selected of extracts derived from hard- and softwood with a 3% GL were approximately pH 1.4, $26.6^{\circ}C$, 43.8 min and approximately pH 0.7, $25.2^{\circ}C$, 24.6 min, respectively. The predicted and experimental values of AA recovery yield were similar whilst sugar retention was 100%.

• The Korean Journal of Mycology
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• v.41 no.3
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• pp.167-171
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• 2013

The goal of this study was to screen a useful alcohol fermentative yeast for Gugija leaf makgeolli (Gl makgeolli) brewing and establish its optimal fermentation condition. Gugija leaves with various alcohol fermentative yeasts were added into the mixture of cooked non-glutinous rice and koji, and then fermented at $25^{\circ}C$ for 7 days. Among several Gl makgeolli, ethanol contents was the highest in Gl makgeolli made by S. cerevisiae F-1. Therefore, we selected S. cerevisiae F-1 as suitable yeast for brewing of Gl makgeolli. Gl makgelli with the best total acceptability and high antihypertensive action was obtained when cooked non-glutinous rice (120 g), boiled D.W (100 mL) and JJ koji (60.5 g/300 sp) were mixed and fermented for 2 days at $30^{\circ}C$ with S. cerevisiae F-1 (5%), and added again cooked non-glutinous rice (150 g), glutinous rice (100 g), D.W (500 mL) and Gugija leave (0.1%/cooked rice) and further fermented for 5 days at $25^{\circ}C$.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.10a
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• pp.9-15
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• 1995

When quiescent cells ate stimulated to enter the cell cycle, the thymidine kinase(TK) gene is transcriptionally activated at the border of Gl and 5. In this report we show that the human TK promoter contains multiple protein-binding sites. By site-directed mutagenesis, we identified a protein-binding site on the human TK promoter requited for conferring Gl-S-regulated transcription to a heterologous promoter and dissociated it functionally from an adjacent protein-binding domain containing an inverted CCAAT motif requited for high basal level expression. Substitution-mutation of this site results in constitutive expression of the neo reporter gene in serum-stimulated fibroblasts, as well as in cells arrested in mid-Gl by a temperature-sensitive mutation. The regulatory domains for the human TK promoter exhibit interesting symmetrical features, including a set of CCAAT motifs and sites similar to the novel Yi protein-binding site recently discovered in the mouse TK promoter. Thus, components of the hTK complex is important for hTK gene regulation.

• Journal of Convergence for Information Technology
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• v.10 no.5
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• pp.232-238
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• 2020

This study evaluated the anti-aging activity with antioxidant, anti-inflammatory and moisture activity of Ganoderma lucidum spore oil(GLS). GLS increased DPPH radical scavenging activity in a dose-dependent manners. Anti-inflammatory assay measured the inhibitory effect of GLS on NO, TNF-α and IL-6 production in LPS-stimulated RAW264.7 cells. As a result GLS inhibited NO and pro-inflammatory cytokine, TNF-α, IL-6 production. Also using human fibroblast cell to the procollagen production analysis and COL1A1 mRNA expression level analysis for defining, and for AQP-3 mRNA expression level analysis, used human keratinocyte cell. GLS increased procollagen production and COL1A1, AQP-3 mRNA expression. Our results suggest that the GLS have potential anti-inflammatory and wrinkle improves, skin moisture effect.

• Korean Journal of Veterinary Research
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• v.59 no.4
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• pp.181-185
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• 2019

The traditional calculation of gestation length (GL) in Holstein cows relies solely on the insemination day and has a high error rate for actual calving dates. The present study was conducted to develop a more accurate method of estimating GL. Among 3,578 Holstein cows, the number of fetuses had a significant (p < 0.05) effect, with GLs of 282.4 ± 2.5 days for singletons and 276.0 ± 1.6 days for twins. Within the singleton group, the GLs were 284.6 ± 1.9 days for males and 280.3 ± 1.5 days for females, a significant difference (p < 0.05). However, within the twin group, there was no difference in GLs among twin males, twin females, or twins of both sexes. Gestation was significantly shorter for singleton males born from June to August but significantly longer for twin males born in the May to June period (p < 0.05). Neither parity nor housing type was associated with any significant difference in GL. In order to predict the calving dates of Holstein cows accurately and improve their management, calculations should consider the number and sex of fetuses as well as the insemination season.

• Korean Journal of Remote Sensing
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• v.23 no.5
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• pp.401-408
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• 2007

Animated anaglyph is the most cost-effective method for 3D stereo visualization of virtual or actual 3D geo-based data model. Unlike 3D anaglyph scene generation using paired epipolar images, the main data sets of this study is the multi-typed 3D feature model containing 3D shaped objects, DEM and satellite imagery. For this purpose, a prototype implementation for 3D animated anaglyph using OpenGL API is carried out, and virtual 3D feature modeling is performed to demonstrate the applicability of this anaglyph approach. Although 3D features are not real objects in this stage, these can be substituted with actual 3D feature model with full texture images along all facades. Currently, it is regarded as the special viewing effect within 3D GIS application domains, because just stereo 3D viewing is a part of lots of GIS functionalities or remote sensing image processing modules. Animated anaglyph process can be linked with real-time manipulation process of 3D feature model and its database attributes in real world problem. As well, this approach of feature-based 3D animated anaglyph scheme is a bridging technology to further image-based 3D animated anaglyph rendering system, portable mobile 3D stereo viewing system or auto-stereo viewing system without glasses for multi-viewers.

• The Journal of Engineering Geology
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• v.29 no.4
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• pp.541-552
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• 2019

In this study, landslide flume tests were performed to analyze characteristics of ground characteristics and landslide occurrence due to rainfall infiltration. As test materials, weathered granite soil and gneiss soil, the most frequent landslides in Korea, were used, and landslides were triggered by heavy rain (Intensity = 200 mm/hr). The measurement sensors were installed with 3 sets at toe, slope, top part and shallow (GL-0.2 m), middle (GL-0.4 m), and deep (GL-0.6 m) depth in the slope and measured at 10 second intervals. After landslide flume tests, the slope stability analysis was performed by applying the unsaturated soil theory based on the change of ground characteristics and compared with actual landslide occurrence from flume test. As a result of the analysis, factor of safety reflected the landslide occurrence from flume test and factor of safety decreased as rainfall infiltration, leading to slope failure. Finally we compared to the factor of safety below 1 and actual landslide occurrence time, the average difference was 1,600 seconds for weathered granite soil and 5,400 seconds for weathered gneiss soil.