• Journal of Korean Society for Geospatial Information Science
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• v.22 no.4
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• pp.21-30
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• 2014

Lately, new influentials have secured a large number of volunteers on social networks due to vitalization of various social media. There has been considerable research on these influential people in social networks but the research has limitations on location information of Location Based Social Network Service(LBSNS). Therefore, the purpose of this study is to propose a spatial detection methodology and application plan for influentials who make comments about diverse social and cultural issues in LBSNS using spatial statistical analysis methods. Twitter was used to collect analysis object data and 168,040 Twitter messages were collected in Seoul over a month-long period. In addition, 'politics,' 'economy,' and 'IT' were set as categories and hot issue keywords as given categories. Therefore, it was possible to come up with an exposure index for searching influentials in respect to hot issue keywords, and exposure index by administrative units of Seoul was calculated through a spatial joint operation. Moreover, an influential index that considers the spatial dependence of the exposure index was drawn to extract information on the influential areas at the top 5% of the influential index and analyze the spatial distribution characteristics and spatial correlation. The experimental results demonstrated that spatial correlation coefficient was relatively high at more than 0.3 in same categories, and correlation coefficient between politics category and economy category was also more than 0.3. On the other hand, correlation coefficient between politics category and IT category was very low at 0.18, and between economy category and IT category was also very weak at 0.15. This study has a significance for materialization of influentials from spatial information perspective, and can be usefully utilized in the field of gCRM in the future.

• IMMUNE NETWORK
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• v.13 no.6
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• pp.295-300
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• 2013

Der f 2 is the group 2 major allergen of a house dust mite (Dermatophagoides farinae) and its function has been recently suggested. To determine the optimal condition of sensitization to recombinant Der f 2 (rDer f 2) in murine model of asthma, we compared the effectiveness with different adjuvants in BALB/c and C57BL/6 mice. Mice from both strains sensitized with rDer f 2 by intraperitoneal injection or subcutaneous injection on days 1 and 14. The dosage was $20{\mu}g$. Freund's adjuvants with pertussis toxin (FP) or alum alone were used as adjuvants. On days 28, 29, and 30, mice were challenged intranasally with 0.1% rDer f 2. We evaluated airway hyperresponsivenss, eosinophil proportion in lung lavage, airway inflammation, and serum allergen specific antibody responses. Naive mice were used as controls. Airway hyperresponsiveness was increased in C57BL/6 with FP, and BALB/c with alum (PC200: $13.5{\pm}6.3$, $13.2{\pm}6.7$ vs. >50 mg/ml, p<0.05). The eosinophil proportion was increased in all groups; C57BL/6 with FP, BALB/c with FP, C57BL/6 with alum, BALB/c with alum ($24.8{\pm}3.6$, $20.3{\pm}10.3$, $11.0{\pm}6.9$, $5.7{\pm}2.8$, vs. $0.0{\pm}0.0$%, p<0.05). The serum allergen specific IgE levels were increased in C57BL/6 with FP or alum (OD: $0.8{\pm}1.4$, $1.1{\pm}0.8$, vs. $0.0{\pm}0.0$). C57BL/6 mice were better responders to rDer f 2 and as for adjuvants, Freund's adjuvant with pertussis toxin was better.

• Journal of Food Hygiene and Safety
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• v.34 no.5
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• pp.489-494
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• 2019

The appropriateness of introducing blockchain technology into food safety management systems was evaluated by conducting a survey of experts on the effectiveness and constraint evaluation indicators, and a portfolio analysis was conducted to set the priorities of blockchain application. The food safety management activities considered in this study were issuing documents on food import/export, food hygiene rating scheme, civil complaint management in the food sector, food- related certification, risk information management, and food traceability systems. The sectors that can be expected to be effective in the introduction of blockchain technology were food- related certification, food hygiene rating scheme, risk information management, and issuing documents on food import/export. In the case of food traceability systems and civil complaint management, the introduction of blockchain technology was not recommended due to ineffectiveness. From the evaluation of the constraints (e.g., technical limits, cost, legal amendment, personal information disclosure, timeliness, and ease of connection) to be overcome when introducing blockchain into food safety management, it was found that there are more than average constraints in all six areas. In particular, the food traceability system was evaluated to have the most constraints. Issuing documents on food import/export is very effective with the introduction of blockchain technology, but due to high cost and legal restrictions, it is necessary to improve the institutional system in order to introduce blockchain. Among the evaluation sectors, food- related certification, food hygiene rating scheme, and risk information management on foods were suitable for preferentially adopting blockchain technology since these areas might experience greatly improved reliability and transparency through the introduction of blockchain, with relatively low constraints.

• Journal of Korean Society of Water and Wastewater
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• v.33 no.5
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• pp.353-366
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• 2019

This research proposes an optimal flushing operation technique in an effort to prevent secondary water pollutions and accidents in aged pipes, and to improve the cleaning effect of unidirectional flushing. Water flow directions were analyzed using EPANET 2.0, while flushing and air scouring experiments in forward and reverse directions were performed in the field. In 42 experiments, average residual chlorine concentration and turbidity were improved after cleaning compared to before cleaning. It was found that even when the same cleaning method was used, further improvement of cleaning effect was possible by applying air injection and reverse direction cleaning techniques. By means of one-way ANOVA(Analysis of variance), it was also possible to statistically verify the need of actively utilizing air injection and reverse direction cleaning. Based on correlation between turbidity and TSS, the total amount of suspended solids removal was estimated for 874 flushing operations and 194 air scouring operations. The result showed that air scouring used more discharge water than flushing by an average of $4.9m^3$ yet with larger amounts of suspended solids removal by an average of 145.9 g. The result of analysis on turbidity values from 887 flushing operations showed low cleaning effect of unidirectional flushing for the pipes with diameters over 300 mm. In addition, the turbidity values measured during cleaning showed an increasing tendency as pipe age increased. The methodology and results of this research are expected to contribute to the efficient maintenance and improvement of water quality in water distribution networks. Follow-up research involving the measurement of water quality at regular time intervals during cleaning would allow a more accurate comparison of discharge water quality characteristics and cleaning effects between different cleaning methods. To this end, it is considered necessary to develop a standardized manual that can be used in the field and to provide relevant trainings.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.5
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• pp.114-126
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• 2021

This paper compared bridge maintenance in United Stated, United Kingdom, Japan, and Korea. Bridges play an essential role in transportation network and in the economic production process. To provide a desirable level of service to the public within limited budgets, it is required to provide effective bridge maintenance activities (e.g. inspection and repair/rehabilitation) at acceptable level of bridge service. A number of bridges are expected to age rapidly in Korea, which will be the excess burden of government. Since several countries have experienced a number of deteriorated bridges because of aging, the countries aforementioned in this study have already developed comprehensive bridge maintenance programs such as inspection practice and repair/rehabilitation techniques. Therefore, the goal of this paper is to synthesize and to compare useful knowledge on bridge maintenance and bridge crack repair/restoration of deteriorated concrete bridge in the four countries. Finally, recommendations that will serve as guidance to transportation agencies for potential enhancements to bridge maintenance and bridge repairs are presented.

• Journal of Animal Science and Technology
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• v.60 no.12
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• pp.32.1-32.10
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• 2018

Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

• IMMUNE NETWORK
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• v.1 no.3
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• pp.213-220
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• 2001

Background: A previous study has shown that Euonymus alatus (EA) has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to liver injury by an initial protection against drug-induced hepatocyte demage. The present study was undertaken to evaluate the protective effect of EA-extract on experimentally induced hepatitis in ICR mice and to investigate some mechanisms responsible for its action. Methods: Water EA extract was used in this experiments. The mice received i.p. a dose of 700 mg/kg galactosamine (GalN) together with $5{\mu}g/kg$ of endotoxin (LPS), or received i.v. 12 mg/kg of concanavalin A (Con A). EA (4 mg/mouse) was administrated on day -2, -1 and 0 before induction of liver injury. Liver injury was assessed by measurement of serum alanin amino-transferase (SGPT) levels on 9 hr after GaIN.LPS, or 8 hr after con A administration. Results: Treatment with either GaIN or LPS alone did not cause hepatitis. However, simultaneous administration of GalN and LPS to mice resulted in LPS-dose dependent fulminant hepatitis. GaLN/LPS-induced liver injury was reduced when mice were given EA for 3 days before induction. This preventive effect of Ea was more prominent when EA was given by intraperitoneal route rather then by oral route. Pretreatment of EA or dexamethasone inhibited significantly $TNF{\alpha}$ production in GalL/LPS-injured mice. However, EA-treatment did not influence $TNF{\alpha}$-induced hepatitis in GalN-sensitized mice, suggesting that $TNF{\alpha}$ is likely to act as one of final mediators of endotoxin action and the protective effect of EA might be manifested chiefly by inhibition of endotoxin-induced $TNF{\alpha}$ production, not by blocking the $TNF{\alpha}$-action. Injection of Con A into mice evoked remarkable liver injury in a dose dependent fashion. This liver damage was reduced by EA-pretreatment. Dexamethasone significantly reduced both GalL/LPS-induced and Con A-induced liver damages, showing synergism with EA. However, indomethacin reduced only GalN/ LPS-induced hepatitis, not for Con A-induced hepatitis. Conclusion: These results led to the conclusion that EA may be able to contribute at least in part to prevent the drug-induced hepatotoxicity, and that its anti-hepatitis effects might be manifested directly by modulation of endogenous mediators, such as leukotriese D4, $TNF{\alpha}$ and free radical, and indirectly by regulation of immune mediated responses. Also these results suggested that EA could be developed as a potential antidotic agent.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.1-8
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• 2013

COP9 signalosome (CSN), which is originally identified as the regulator of the photomorphogenic development in plant, is highly conserved protein complex in diverse eukaryotic organisms. Most eukaryotic CSN complex is composed of 8 subunits, which is structurally and functionally similar to the lid subunit of 26S proteasome and eIF3 translation initiation complex. CSN play important functions in the regulation of cell cycle and checkpoint response by controlling Cullin-Ring E3 ubiquitin ligases (CRL) activities. CSN exhibits an isopeptidase activity which cleaves the neddylated moiety of cullin components. In fission yeast, S-phase cell cycle progression was delayed and the sensitivity to g-ray or UV was increased in CSN1 and CSN2 deletion mutants, indicating that yeast CSN is also involved in the checkpoint regulation. CSN in fungal system more closely resembles that of the higher organisms in the structure and assembly of their components. Functionally, CSN is associated with the regulation of conidiation rhythms in Neurospora crassa and the sexual development in Aspsergillus nidulans. Recent studies also revealed that CSN functions as an essential cell cycle regulator, playing key roles in the regulation of DNA replication and DNA damage response in Aspergillus. Overall, CSN of microorganisms, such as fission yeast and fungi, share functionally common aspects with higher organisms, implying that they can be useful tools to study the role of CSN in the CRL-mediated diverse cellular activities.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1565-1569
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• 2008

지난 수십 년 동안, 아시아 태평양 지역 각국은 수자원 접근성 및 인프라의 확충을 포함한 물관리의 다양한 측면에서 현저히 발전하였다고 평가되고 있다. 그러나 급속한 인구 증가 및 경제 성장, 도시화, 기존 수자원의 고갈 등은 아태 지역의 물 수요가 여전히 충족시키지 못하는 원인이다. 또한 기후변화로 인한 환경적 변화를 고려하여 모든 수자원정책의 입안 및 계획이 이루어져야 한다. 경제적 성장률은 전반적으로 높았지만 빈곤 문제는 역시 도시나 농촌을 구분할 것 없이 만성적인 문제로 남아 있으며, 아태 지역 개도국 인구의 16%가 영양실조 상태이며, 2015년까지 그 수를 반감하자는 밀레니엄 개발목표(Millenium Development Goals, MDGs) 달성은 아직 갈 길이 멀다. 또한 아태 지역은 물 관련 재해에 대해 전 세계에서 가장 취약한 지역이며 그로 인한 지속가능한 발전이 지체되고 있다. 1960년부터 2006년까지 물 관련 재해로 인한 전 세계 사상자의 80%에 달하는 60만 명의 사상이 아태 지역에서 발생하였으며, 80억 달러의 경제적 손실이 있었다. 홍수, 가뭄, 쓰나미, 폭풍, 해일 등으로 인한 사회경제적 피해는 최근 들어 더욱 확산되고 있다. 지난 10년간의 급속한 인구 증가로 많은 인구가 범람지대 및 기타 재해 취약 지역에 거주하게 되었으며, 이는 물 인프라 확충을 위한 많은 투자를 요하고 있다. 2006년 3월 멕시코에서 개최된 제4차 세계물포럼의 지역별 추진과정에서 아태 지역은 생태계, 인구, 정치적 제도 및 사회경제적 환경 등 각국의 다양성이 이와 같은 공통된 물문제 해결에 기반이 될 수 있는 요소임에 공감하고 지속적인 수자원 확보와 올바른 수자원관리에 공동의 노력을 기울여 효율적인 메커니즘을 구성을 통해 아태 지역의 사회경제적 발전을 촉구하기로 하였다. 이에 아시아 태평양 물포럼(Asia-Pacific Water Forum)이라는 새로운 이니셔티브가 구성되었고 물 관련 재원 조달 및 인프라 확보를 통해 국경을 초월하는 협력을 증진하고 재해 관리와 조화로운 수자원 개발과 생태 보호등을 위한 일관된 수자원정책 수립과 프로그램 개발 등을 목표로 설정하여 활동을 시작하였다. 아시아 태평양 물정상회의(이하 아태 물정상회의, Asia-Pacific Water Summit(APWS))는 이러한 목표 달성을 구체화하기 위해 각국의 지도자 및 정책입안자에 밀레니엄 개발목표(MDGs) 달성을 위해 물 문제가 얼마나 중요한지 부각시키고 실질적인 정책 결정에 기여하기 위해 제안되었다. 제1차 아태 물정상회의가 2007년 12월 3-4일 양일에 걸쳐 일본 벳부에서 개최되었고, 2008년 G8 정상회담이나 2009년 제5차 세계물포럼에 직접적으로 연계되어 지역 협력을 주도한 가운데 아태 지역 뿐 아니라 전 지구적으로 인류 및 사회 발전을 위한 이정표로 새로운 수자원 네트워크 발돋움의 계기가 되었다.

• IMMUNE NETWORK
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• v.5 no.4
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• pp.237-246
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• 2005

Background: Little information is available on the identification and characterization of the upstream regulators of the signal transduction cascades for Mycobacterium tuberculosis (M. tbc)-induced ERK 1/2 activation and chemokine expression. We investigated the signaling mechanisms involved in expression of CCL3 /MIP-1 and CCL4/MIP-1 in human primary monocytes infected with M. tbc. Methods: MAP kinase phosphorylation was determined using western blot analysis with specific primary antibodies (ERK 1/2, and phospho-ERK1/2), and the upstream signaling pathways were further investigated using specific inhibitors. Results: An avirulent strain, M. tbc H37Ra, induced greater and more sustained ERK 1/2 phosphorylation, and higher CCL3 and CCL4 production, than did M. tbc H37Rv. Specific inhibitors for mitogen-activated protein kinase (MAPK) kinase (MEK; U0126 and PD98059) significantly inhibited the expression of CCL3 and CCL4 in human monocytes. Mycobactetia-mediated expression of CCL3 and CCL4 was not inhibited by the Ras inhibitor manumycin A or the Raf-1 inhibitor GW 5074. On the other hand, phospholipase C (PLC) inhibitor (U73122) and protein kinase C (PKC)specific inhibitors ($G\ddot{o}6976$ and Ro31-8220) significantly reduced M. tbc-induced activation of ERK 1/2 and chemokine synthesis. Conclusion: These results are the first to demonstrate that the PLC-PKC-MEK-ERK, not the Ras-Raf-MEK-ERK, pathway is the major signaling pathway inducing M. tbc-mediated CCL3 and CCL4 expression in human primary monocytes.