• BMB Reports
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• 제37권4호
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• pp.474-479
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• 2004

The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ~21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.

• 한국잠사곤충학회지
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• 제51권2호
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• pp.207-210
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• 2013

본 연구는 누에 숙잠 혈림프의 멜라닌 생성 억제 효과에 대해 알아보기 위해 수행되었다. 실샘을 제거한 누에 숙잠에서 혈림프를 정제하였고, 이의 항산화, 멜라닌 생성 억제, 티로시나아제 활성 억제 효과 여부를 실험한 결과는 다음과 같다. 1. 숙잠의 실샘을 제거한 후, 나머지 누에조직을 분쇄하여 원심분리 후 여과하였던 기존의 방식에서 연속식 원심분리로 정제 방법을 개선한 결과, 수율은 53% 증가하였고 정제기간은 5일 단축되었다. 2. 누에 숙잠 혈림프의 DPPH 자유라디칼 소거능을 측정한 결과 비타민 C 보다는 효과가 떨어졌지만 세리신 단일 물질보다 높은 효과를 나타내었다. 3. 숙잠 혈림프 단백질을 음이온 교환 크로마토그래피로 분리하여 활성산소 억제 효과를 측정한 결과, 70 kDa과 30 kDa 단백질이 모두 포함되어 있는 혈림프 단백질(상등액)이 대조군보다 2배, 70 kDa 단백질이 30 kDa 단백질에 비해 약 1.7배 활성산소 억제 효과를 나타냄을 확인하였다. 4. 누에 숙잠 혈림프의 멜라닌 생성 억제 효과는 미백화장품 소재로 널리 사용되는 상백피와 알부틴보다 각각 9.15%, 11.56% 더 우수함을 확인하였다(혈림프 1% 농도 기준). 5. 티로시나아제 활성 억제 효과는 대조인 상백피보다 누에 숙잠 혈림프의 티로시나아제 활성 억제 효과가 우수하였으나 단일 물질인 알부틴보다는 억제 효과가 떨어지는 결과를 나타내었다. 하지만 정제 혈림프 농도 $450{\mu}g/m{\ell}$에서는 90%에 이르는 티로시나아제 활성 억제 효과를 확인하였다.

• 전기전자학회논문지
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• 제17권4호
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• pp.407-417
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• 2013

본 논문에서는 LED 조명 통신을 위한 재구성형 주변장치유닛을 제안한다. 조명시스템을 위한 임베디드 장치에서는 다양한 통신 프로토콜이 요구된다. 이러한 통신 프로토콜로는 UART, SPI, IrDA 등의 직렬 통신, DALI나 DMX512와 같은 조명제어 통신이 있다. 다양한 통신 프로토콜 요구사항을 각 IP로 개별 구현하여 만족시킬 경우 비용 및 전력 효율이 떨어질 수 있다. 본 논문에서는 각 프로토콜의 신호 형식을 분석하여 UART, SPI, IrDA, DALI, DMX512의 통신 기능을 통합하는 방법을 제안한다. 제안한 재구성형 주변장치유닛은 개별 구현 방법에 비해 57% 감소된 양의 게이트수를 사용한다. 또한 본 논문에서는 조명 네트워크를 유연하게 구현하기 위해 사용될 수 있는 매핑테이블 기반의 DALI-ZigBee 인터페이싱 방법을 제안한다. 이 방법을 사용하면 DALI와 ZigBee를 혼용한 다양한 구성의 조명 네트워크 시스템을 효율적으로 구현할 수 있다. LED 조명시스템 플랫폼을 구성하여 유무선 조명 통신 네트워크의 동작을 검증하였다. 본 논문에서 제안한 재구성형 주변장치유닛과 DALI-ZigBee 인터페이싱 방법은 유무선 조명시스템을 효율적으로 구현하는 데에 사용될 수 있다.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.56-57
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• 2005

• KSBB Journal
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• 제25권3호
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• pp.257-264
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• 2010

Bone morphogenetic protein-7 (BMP-7) is one of important growth factors for skeletal development and bone growth. In this work, BMP-7 was efficiently expressed in recombinant Bacillus subtilis. The mature BMP-7 protein indicated molecular weight of 15.4 kDa by Western blot assay and was secreted into culture medium with 0.35 ng/mL. The extracellular and intracellular rhBMP-7 proteins were purified by using a FPLC system with an ion exchange column and a gel filtration column. The extracellular and intracellular rhBMP-7 proteins had finally a 57.1% purity and a 36.2% purity, respectively. The purified rhBMP-7 proteins showed an intact biological activity which stimulated alkaline phophatase (ALP) activity in MC3T3-E1 cells.

• Fisheries and Aquatic Sciences
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• 제5권1호
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• pp.21-27
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• 2002

Angiotensin I converting enzyme inhibitory activities of shelled krill (Euphausia superba) hydrolysates by autolysis and by hydrolysis with commercial proteases were analyzed. Among the proteases, Alcalase was the most effective protease for the hydrolysis of krill considering the degree of hydrolysis $(87.5\%)$ and the ACE inhibitory activity $(60\%)$. Four hour hydrolysis suggested as the most suitable and economic. In order to establish the optimum hydrolysis condition of krill, degree of hydrolysis and ACE inhibitory activity as affected by Alcalase concentration and water amount added were statistically analyzed by response surface methodology (RSM). The optimum hydrolysis condition was $2.0\%$ Alcalase hydrolysis in 2 volumes (v/w) of water at $55\% for 4 hr. The hydrolysate prepared from the optimum hydrolysis condition was fractionated by molecular weight. The lower molecular weight fraction showed the higher ACE inhibitory activity. $IC_{50}$ of the fraction under 500 Da was 0.57mg protein/mL.

• Mycobiology
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• 제48권1호
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• pp.51-57
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• 2020

A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.

• Journal of Korean Dental Science
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• 제7권1호
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• pp.25-30
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• 2014

Subcutaneous emphysema and pneumomediastinum is a relatively uncommon phenomenon. It may occur secondary to dental treatment using high-speed air turbine handpieces, especially after extraction of tooth. Subcutaneous emphysema is often limited only to the areas of head and neck, but also can involve deeper structures. Thorough examination and conservative treatment of these problems are essential in preventing life-threatening complications such as airway obstruction and mediastinitis. The subject of this report is a 57-year-old woman with subcutaneous emphysema and pneumomediastinum during the extraction of maxillary third molar using high-speed air turbine handpiece. If there isn't any appropriate measure, severe complications may occur. Therefore it is important to be well-informed of proper diagnosis and treatment. This article shall present a case report with literature review.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(D)
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• pp.54-57
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• 2011

안드로이드와 같이 패턴 잠금을 기본으로 하는 스마트폰 OS가 보급 되면서 패턴 잠금이 널리 사용되고 있다. 그러나 현재의 패턴 잠금 시스템은 패턴 입력의 실수와 악의적인 접근에 의한 입력에 대해 동일한 페널티를 부여하기 때문에 보안에 취약한 문제점을 가지고 있다. 본 논문에서는 입력 패턴의 분석 방법을 제안하고 분석을 통해, 패턴을 '일치', '부분 일치'와 '불일치'로 분류하여 그에 따른 페널티의 여부를 달리함으로써 보안을 강화하는 방법을 제시한다.

• 대한약리학회지
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• 제23권2호
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• pp.57-75
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• 1987

Two modalities of gonadotropin secretion, pulsatile gonadotropin and preovulatory gonadotropin surge, have been identified in the mammals. Pulsatile gonadotropin secretion is modulated by the pulsatile pattern of GnRH release and complex ovarian steroid feedback actions. The neural mechansim that regulates the pulsatile release of GnRH in the hypothalamus is called "GnRH pulse generator". Ovarian steroids, estradiol and progesterone, appear to exert thier feedback effects both directly on the pituitary to modulate gonadotropin release and on a hypothalamic site to modulate GnRH release; estradiol primarily affects the amplitude while progesterone decreases the frequency of the pulsatile GnRH. Steroid hormones are known to affect catecholamine transmission in brain. MBH-POA is richly innervated by NE systems and close apposition of NE terminals and GnRH cell bodies occurs in the MBH as well as in the POA. NE normally facilitates pulsatile LH release by acting through ${\alpha}-receptor$ mechanism. However, precise nature of facilitative role of NE transmission in maintaining pulsatile LH has not been clearly understood. Close apposition of DA and GnRH terminals in ME might permit DA to influence GnRH release. Action of DA transmission probably is mediated by axo-axonic contacts between GnRH and DA fibers in the ME. Dopamine transmission does not normally regulate pulsatile LH release, but under certain conditions, increased DA transmission inhibit LH pulse. Endogenous opioid acts to suppress the secretion of GnRH into hypophysial portal circulation, thereby inhibiting gonadotropin secretion. However, an interaction between endogenenous opioid peptides and gonadotropin release is a complex one which involves ovarian hormones as well. LH secretion appears to be most suppressed by endogenenous opioids during the luteal phase, at a time of elevated progesterone secretion. The arcuate nucleus contains not only cell bodies for GnRH and ${\beta}-endorphin$ but also a dense aborization of fibers suggesting that GnRH release is changed by the interactions between GnRH and ${\beta}-endorphin$ cell bodies within the arcuate nucleus. The frequency and amplitude of pulsatile LH release seem to be increased during the preovulatory gonadotropin surge. Estradiol exerts positive feedback action on the hypothalamo-pituitary axis to trigger preovulatory LH surge. GnRH is also crucial hormonal stimulus for preovulatory LH surge. It is unlikely, however, that increased secretion of GnRH during the preovulatory gonadotropin surge represents an obligatory neural signal for generation of the LH discharge in primates including human. Modulation of preovulatory LH surge by catecholamines has been studied almost exclusively in rats. NE and E may be involved in distinct way to accumulate GnRH in the MBH and its release into the hypophysial portal system during the critical period for LH surge on proestrus in rats. However, the mechanisms whereby augmented adrenergic transmission may facilitate the formation and accumulation of GnRH in the ME-ARC nerve terminals before the LH surge have not been clearly understood.