• Korean Journal of Microbiology
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• v.16 no.2
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• pp.79-89
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• 1978

The RK-temperate phage which infected with Bacillus cereus was isolated and the characters were investigated. The induction of RK-temperate phage from host bacterium attained by ultraviolet light irradiation (15W, 30cm, 30-120sec) and mitomycin C treatment (0.2-2 ug/ml). The host range of RK-temperate phage was not revealed with lysogenic and related strains of B. cereus. But B. cereus(PS) 352 which obtained by N-nitrosoguanidine treatment(1,000.$\mu$g/ml) to phage infected with host bacteria was sensitive bacteria of RK-temperate phage. RK-temperate phage was stabilized at the condition of nutrient broth (pH 7-8), Tris-buffer (pH 7-8) and ammonium buffer (pH 8-9) and Sorensen's phosphate buffer (pH 6-7), but unstabilized at other salt solutions and pH range. Also, thermostability was to 45.deg.C but unstabilized at above 50.deg.C. At RK-temperate phage, the measurment values of head, neck, mid tail and end tail were 59nm, 9*16nm, 10*189nm, and 10*14nm respectively. The morphology of head was regular polyhedron, and the end tail was coneate form. On the one hand, the number of capsid protein layer of tail were consist of 4, 35, and 1 at neck, mid tail, and end tail, respectively. RK-temperate phage was identified with DNA phage and G+C contents were 38.63. The latent time of RK-temperate phage was 30 minutes and the burst size was 70-80. And the host bacteria was lysed in case of multi-infection, above moi 1.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.291-291
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• 2010

Electron-beam lithography (EBL) process is a versatile tool for a fabrication of nanostructures, nano-gap electrodes or molecular arrays and its application to nano-device. However, it is not appropriate for the fabrication of sub-5 nm features and high-aspect-ratio nanostructures due to the limitation of EBL resolution. In this study, the precision assembly and alignment of DNA molecule was demonstrated using sub-5 nm nanostructures formed by a combination of conventional electron-beam lithography (EBL) and plasma ashing processes. The ma-N2401 (EBL-negative tone resist) nanostructures were patterned by EBL process at a dose of $200\;{\mu}C/cm2$ with 25 kV and then were ashed by a chemical dry etcher at microwave (${\mu}W$) power of 50 W. We confirmed that this method was useful for sub-5 nm patterning of high-aspect-ratio nanostructures. In addition, we also utilized the surface-patterning technique to create the molecular pattern comprised 3-(aminopropyl) triethoxysilane (APS) as adhesion layer and octadecyltrichlorosilane (OTS) as passivation layer. DNA-templated gold nanoparticle chain was attached only on the sub-5 nm APS region defined by the amine groups, but not on surface of the OTS region. We were able to obtain DNA molecules aligned selectively on a SiO2/Si substrate using atomic force microscopy (AFM).

• Analytical Science and Technology
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• v.16 no.6
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• pp.499-503
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• 2003

A high performance liquid chromatography gradient elution method with fluorescence detection for the determination of biotin in pure form and pharmaceutical preparations has been developed. BrMDMC gives intense fluorescence and the fluorescence was monitored with excitation at 360 nm and emission at 410 nm. The calibration curve for biotin shows good linearity over the range of 5 ~ 400 ng with correlation coefficient of 0.999. The detection limit of biotin was 2 ng and the result of recovery was 98.75% with relative standard deviation of 1.1%.

• Journal of the Korean Society for Heat Treatment
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• v.23 no.5
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• pp.245-248
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• 2010

Sn doped $In_2O_3$ (ITO) and ITO/Ni/ITO (INI) multilayer films were deposited on the glass substrates with a reactive magnetron sputtering system without intentional substrate heating and then the influence of the Ni interlayer on the methanol gas sensitivity of ITO and INI film sensors were investigated. Although both ITO and INI film sensors have the same thickness of 100 nm, INI sensors have a sandwich structure of ITO 50 nm/Ni 5 nm/ITO 45 nm. The changes in the gas sensitivity of the film sensors caused by methanol gas ranging from 100 to 1000 ppm were measured. It is observed that the INI film sensors show the higher sensitivity than that of the ITO single layer sensors. Finally, it can be concluded that the INI film sensor have the potential to be used as improved methanol gas sensors.

• Korean Journal of Optics and Photonics
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• v.10 no.2
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• pp.120-127
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• 1999

We discussed two main types-conformal and non-conformal (powered) - of holographic combiner. A theoretical model based on the Kogelnik's coupled wave theory was used to illustrate bandwidth and efficiency properties. Also we showed numerical values for the aberrations that are induced by a wavelength shift from construction to reconstruction and found optimum coordinates to reduce the chrolatic aberation of construction beams using aberration balancing techniques. The holographic combiner manufactured in conformal type with 60$^{\circ}$ incidence angle at 514.5 nm had narrow angular bandwidth (FWHM) of 4.1" and spectral bandwidth of 11.4 nm, while non-conformal one with 50$^{\circ}$, 30$^{\circ}$ incidence angle at 514.5 nm showed spectral and angular bandwidth of 10.7 nm and 5.5$^{\circ}$, respectively.vely.

• Journal of Environmental Science International
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• v.8 no.6
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• pp.699-704
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• 1999

The Photodegradation efficient of total organic compounds in the drinking water has been studied using the methods of photocatalytic reaction and laser beam irradation. The results are summarized as follows; 1. The photodegradation efficiency of total organic compounds shows as $50\%\;to\;80\%$ as within one hour and after this the efficiency is decreased slowly. 2. The photodegradation efficiency of total organic compounds shows as 65 to $90\%$ within 3.3min. when Nd : YAG beam is irradiated to the water layer. 3. An excellent observation of the organic compound removal efficiency gives revealed in that case of the longest wavelength of 532nm is irradiated among the three kinds of laser beam sources of 532nm, 355nm and 266nm. 4. The organic compound removal efficiency shows high in the case of UV beam irradiation in the thin layer of water. However the efficiency is not depended on the thickness of water layer severely. 5. The removal efficiency of the organic compounds in the direct irradiation shows higher than the indirect irradiation in the case of UV beam, but the efficiency is not depended on the direction of irradiation in the case of Nd : YAG beam irradiation.

• Journal of Environmental Science International
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• v.16 no.7
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• pp.793-798
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• 2007

During May of 2003, smoke from fires in the Yucatan Peninsula was transported across the Gulf of Mexico and into Texas where it caused significant enhancement in measured aerosol concentrations and reduced visibility. During this event, the formation and growth of aerosol particles has been observed by a differential mobility analyzer (DMA) / tandem differential mobility analyzer (TDMA) system to characterize the size distribution and size-resolved hygroscopicity of the aerosol. The most number concentration is by the particles smaller than 100 nm, but the integrated number concentrations for over 100 nm increased due to the aerosol growth. Hygroscopic growth factor increase from 1.2 to 1.4 for 25, 50, and 100 nm particles during the nucleating period. This distribution and the aerosol properties derived from the TDMA data were used to calculate the growth rate. Particle growth rates were in the range 1-12 nm/hr.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2008.10a
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• pp.205-210
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• 2008

Titanium-doped indium oxide (ITiO) films were prepared on soda-lime glass substrate using a magnetic null discharge (MND) sputter source. The ITiO thin films containing 10wt.% Ti showed the minimum resistivity of $\rho=5.5{\times}10^{-3}{\Omega}-cm$. The optical transmittance increases from 70% at 450 nm to 80% at 700 nm in visible spectrum. Photoelectron peaks for In 3d, Ti 2p, O 1s and C1s were detected for the ITiO film in the binding energy range of 0 to 1100 eV. The surface roughness of the sample showed a change from 10 nm to 50 nm. The ITiO film used for TCO layer of DSCs exhibited an energy conversion efficiency of about 3.8% at light intensity of 100 mW/$cm^2$.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.498-501
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• 2002

In this paper, we designed double gate(DG) MOSFET structure which has main gate(MG) and two side gates(SG). We have simulated using TCAD simulator. DG MOSFET have the main gate length of nm and the side gate length of 70nm. Then, we have investigated the pinch-off characteristics, drain voltage is changed from 0V to 1.5V at VMG=1.5V and VSG=3.0V. In spite of the LMG is very small, we have obtained a very good pinch-off characteristics. Therefore, we know that the DG structure is very useful at nino scale.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.5
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• pp.725-732
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• 2000

Laser induced incandescence (LII) method is frequently used to measure soot volume fraction in flames. In this study, experiments were performed to measure soot volume fraction in coaxial diffusion flame using LII method and calibrated with laser scattering/extinction method. The effects of laser intensity (>$1{\times}10^8W/cm^2$), laser wavelength (532nm, 1064nm) and detection wavelength (400nm, 600nm) on the LII signal were investigated. On the range of $4{\times}10^8{\sim}8{\times}10^8W/cm^2$ there were no effects of laser intensity on LII signal. Except these ranges, LII signal was increased with laser intensity. For the long gate width, the LII signals of the higher laser intensity (>${\vartheta}(GW/cm^2)$) cases had better correlation with soot volume fraction which were measured by laser extinction method compared with lower laser intensity cases. The errors of 2-dimensional cases at the calibration height were approximately 50% regardless of laser wavelength.