• Clinical and Experimental Reproductive Medicine
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• v.25 no.1
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• pp.65-70
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• 1998

This study was conducted to investigate the effects of Tissue Culture Medium 199 (TCM) and Dulecco's Modified Eagle Medium (DMEM) on the blastulation and grade of human oocytes on Vero cells in vitro. A cohort of 79 and 93 oocytes in metaphase II stage were used in TCM 199 and DMEM respectively. No differences were found in the numer of oocytes showing two-pronuclei between TCM (82.3%) and DMEM (86.0%). The number of fertilized oocytes reaching the blastocyst was not significant in TCM (60.0%) and DMEM (63.1%). A total of 89 blastocysts were categorized into the four grades (BG1, BG2, BG3 and early) depending on their morphology. The number of embryos achieving the blastocyst grade 1 (BG1) was significantly higher (p<0.05) in DMEM (50.8%) than TCM (15.0%). It is concluded that cultured oocytes in DMEM with glutamine on Vero cells should be significantly increased BG1.

• Journal of Pharmaceutical Investigation
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• v.26 no.3
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• pp.175-185
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• 1996

To inhibit the enzymatic degradation of leucine enkephalin (Leu-Enk) and its synthetic analog. $[D-ala^2]$-leucine enkephalinamide (YAGFL), in the nasal, rectal and vaginal mucosal and serosal extracts of rabbits, effects of enzyme inhibitors such as amastatin (AM), puromycin (PM), thiorphan (TP), thimerosal (TM), EDTA, N-carboxymethyl-Phe-Leu (CPL), phenylethyl alcohol (PEA), phenylmercuric acetate (PMA), benzalkonium chloride (BC) and modified cyclodextrins, alone or in combination, were observed by assaying the pentapeptides staying intact during incubation. Mucosa extracts were prepared by exposing freshly-excised mucosal specimens mounted on Valia-Chien cells to isotonic phosphate buffer while stirring. The degradation of Leu-Enk and YAGFL followed the apparent first-order kinetics. The half-lives (mean) in the nasal, rectal and vaginal mucosal extracts were found to be 1.07, 0.33 and 1.14 hr for Leu-Enk, and 16.9, 6.2 and 6.8 hr for YAGFL, respectively. AM or PM, which is an aminopeptidase inhibitor, did not show a sufficient inhibition of Leu-Enk $(50\;{\mu}g/ml)$ degradation in all kinds of extracts. $Dimethyl-{\beta}-cyclodextrin\;(DM-{\beta}-CyD)$ decreased the degradation rate constants of Leu-Enk about 2 or 3 times, comparing with no additive. However, the use of mixed inhibitors of AM $(50\;{\mu}M)$/TM (0.25 mM)/EDTA (5 mM) resulted in a full stabilization of Leu-Enk by decreasing the degradation rate constants 67.3, 161.3 and 113.8 times far the nasal, rectal and vaginal mucosal extracts, respectively, comparing with no inhibitor. With mixed inhibitors, Leu-Enk remained intact more than 90% after 6 hr-incubation. In the stabilization of YAGFL, hM, TP or CPL alone showed little efffct, and some additives demonstrated a considerable inhibition of YAGFL degradation in the rank order of TM > BC > EDTA. However, the addition of mixed inhibitors such as TM (0.5 mM) and EDTA (5 mM) into the extracts protected YAGFL from the degradation by more than 85% even after 24 hr-incubation, suggesting almost complete inhibition of YAGFL degradation in the extract. On the other hand, $DM-{\beta}-CyD\;or\;hydroxypropyl-{\beta}-cyclodextrin$ (10%) were also found to retard enzymatic degradation rates of YAGFL markedly, and resulted in staying intact more than 80% of YAGFL in the nasal and vaginal mucosal extracts, and more than 60% in the rectal mucosal extract after 16 hr-incubation.

• Journal of agricultural medicine and community health
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• v.21 no.2
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• pp.209-220
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• 1996

This study was performed to investigate the prevalence of chronic disease and utilization of medical facility in rural area. 5,797 persons were randomly selected from 28,883 rural residents who were over 30 years old and living in Moonkyung city in Kyungsangpook Province during the period October 1 1983 to February 28 1994. The person prevalence rate was 336 per 1,000. The prevalence rate in men(278 per 1,000) was higher than that(388 per 1,000) in women. The spell prevalence rate in men(367) was significantly higher than that(429) in women (p<0.05). The prevalence was increased with the age, 106 in the 30's. 223 in the40's, 336 in the 50's, 407 in the 60's, and 457 in the age group of 70 above. Of all chronic diseases, the prevalence of neuralgia ranked first(128), chronic gastrointestinal disorders(64), degenerative arthritis(54). hypertension (44) and diabetes (14) were followed in descending order. In men, the prevalence of neuralgia ranked first, chronic gastrointestinal disorders, degenerative arthritis, hypertension and bronchial asthma were followed in descending order. In women, the prevalence of neuralgia ranked first, degenerative arthritis, chronic gastrointestinal disorders, hypertension and diabetes were followed in descending order. The prevalences of neuralgia, degenerative arthritis and hypertension were significantly higher in women than those in men, however, those of cardiovascular disease, pulmonary tuberculosis and liver cirrhosis in men were significantly higher than those in women(p<0.05). Most patients with chronic diseases were more likely to utilize hospital and/or local clinics than public health facility or community health providers. Patients with neuralgia, degenerative arthritis and bronchial asthma tended to utilizing local clinics initially, however, they were changed to visit public health facility or community health providers afterwards. Patients with hypertension or pulmonary tuberculosis were more likely to visit public health facility, however, those with diabetes, cancer, heart failure, CVD, liver cirrhosis were more likely to visit hospital.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.24-29
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• 1999

The physicochemical properties of tagatose, a low calorie sweetener, was investigated. Rheological property of tagatose solution was found to be Bingham fluid. As the concentration of tagatose increased from 10 to 50% at $25^{\circ}C$, the viscosity increased from 1.65 to 5.14 cp. When the temperature of 40% tagatose solution increased from 15 to $55^{\circ}C$, the viscosity decreased from 4.59 to 2.33cp. The melting onset temperature and endothermic enthalpy of tagatose were $130.4^{\circ}C$ and -202.3 J/g, respectively, which were obtained from the analysis of differential scanning calorimetry. Tagatose showed higher water absorption than sucrose under $85{\sim}100%$ of relative humidity. Tagatose was less soluble than sucrose at $20{\sim}70^{\circ}C$. Water activity of tagatose in 60% concentration was 0.892, which was lower than 0.957 of sucrose solution. Tagatose solution adjusted from pH 2 to pH 12 was stable after 3 days. Amount of tagatose was not changed after heat treatment at $154^{\circ}C$ for 4 hours. But a browning reaction was found and absorbance of a tagatose solution increased with heat treatment.

• The Korean Journal of Rehabilitation Nursing
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• v.1 no.1
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• pp.51-60
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• 1998

The result of studying papers about management of lower back pain published in domestic and foreign nursing and medical magazines in these 10 years is as follows ; 1. General characteristic of lower back pain ; 1) In sex distribution, there were more men than women in 5 papers and more women than men in 4 papers among 9 papers surveryed. 2) In age distribution, thirties to forties of patients had more lower back pain as surveyed by general hospitals and fifties to sixties of patients had more lower back pain as surveyed by oriental medical hospitals. 3) In cause factor, there were 50 to 65% of sprain, 32 to 44% of herniated intervertebral disc and 13 to 29% of degenerative changes. 4) In symtom distribution, there were 26 to 57% of lower back pain, 42 to 65% of lower back pain with radiating pain and 34 to 99% of paravertevral muscle spasm. 5) In period of pain management distribution, 18 to 40% of patients experienced pain for less than 6 months and 59 to 82% of them experienced pain for more than 6 months in 3 papers among 4 papers. 6) In surveying the treatment, 66 to 88% of patients had conservative treatment and there were treatments of general hospital, oriental medicine, self remedy and traditional practice in conservative treatment. 7) In job distribution, 12 to 50% of them were housekeepers, 23 to 31% office workers, 4.6 to 36% blue color workers and 11 to 15% students. 2. As psychological character lower back pain paients had anxiety, depression, anger-hostility, phobic anxiety, neurasthenia, hypochondriasis, and interpersonal sensitivity. 3. To distinguish the cause of lower back pain, plain lumbar roentgenogram, straight leg rasing test, eletromyelogic findings, somatosensory evoked potentials CT and MRI were performed. 4. To relieve lower back pain. epidural adhesiolysis, epidulal injection of local anesthetic in mixture with steroid, lumbar spinal root block, low level laser therapy, acupuncture like transcutaneous nerve stimulation(AL TENS), topical capsaicin and lumbar orthotics were used in medical field, and relaxation technique was used in nursing field. 5. Mckenzie's extension exercise and William's flexsion exercise for lower back pain were used in medical field and Yoga exercise was applied in nursing field. 6. The more school education and self efficacy were high, the better they had active coping lower back pain positively and the less self efficacy was the more they had serious pain. As a result of studying the paper there have been very little research for lower back pain in nursing fields of Korea and foreign countries. Because 60 to 80% of population expeience lower back pain at least more than once, it is necessary to develop the study and clinical practice for management of lower back pain.

• Korean journal of food and cookery science
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• v.26 no.6
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• pp.707-716
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• 2010

Germinated brown rice is a useful material that contains natural nutrients and biologically active substances. This study was conducted to develop a optimal composite recipe for functional germinated brown rice cookie with added spinach (spinacia oleracea L) powder and with high preference in all age groups. The experimental design utilized herein was based on the Central composite design methodology of response surface, which included 16 experimental points, including 2 replicates for spinach, sugar, and butter. The physical, mechanical, and sensory properties of the test were measured, and these values were applied to the mathematical models. A canonical form and perturbation plot showed the influence of each ingredient on the mixed final product. The results of spread ratio decreased significantly with increases in spinach powder, sugar, and butter(p<0.01). L value shows that lightness decreased significantly with an increase in sugar(p<0.05), but the a and b values were not significant. Sensory evaluation found significant values for color(p<0.05), flavor(p<0.05), texture(p<0.05) and overall quality(p<0.05) in the predicted model. The optimum formulation by the numerical and graphical methods was calculated as follows: spinach powder 3 g, sugar 35.5 g butter 54.4 g.

• Journal of Korea Entertainment Industry Association
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• v.13 no.2
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• pp.279-287
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• 2019

This study is a descriptive study to dentify gerotranscendence, family support, social support, self-esteem, and degree of fear of death and the relationship between old age. Through this study, it provide basic data that can help target elderly people do better in their later years. From June 2018 to October 2018, residents' self-governing centers, senior citizens' centers, and life-long education centers were visited to explain the purpose of the research, and those who voluntarily agreed to the research were selected. The following results were obtained by surveying 50 adults aged 60 or older in Seoul using structured questionnaire. The difference in the level of gerotranscendence, family support, social support, self-esteem, and fear of death according to the general characteristics of the target was analyzed as t-test and ANOVA. The correlation of gerotranscendence, family support, social support, self-esteem, and fear of death in early years of life was analyzed as Pearson correlation coefficient. Among the common traits, age was related to social support and death anxiety, marital status was related to family support, and life satisfaction was related to family support. There was a correlation between old age and fear of death, a correlation between family support and self-esteem, and social support related to self-esteem and fear of death. In conclusion, this study found that early retirement in the old age was associated with fear of death, and it was found that family support in the old age affected self-esteem and satisfaction in life. Future studies need to be conducted on groups of adults in the later years, including the degree of elderly age, satisfaction with living, and relationship between the elderly and the elderly.

• Journal of Families and Better Life
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• v.32 no.1
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• pp.151-166
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• 2014

This study attempted to explore how middle aged married men and women prospected a Centenarian society and what implications their prospect cast for family policy. We conducted focus group interviews with five groups in order to identify their subjective prospects on marital relations, parent-child relations, caregiving from family or institutions, and alternative living arrangement. From those interviews, we found that married men and women in their 40s, 50s, and 60s possessed ambivalent attitudes toward their marital relationship, either acknowledging an importance of marital relationship or accepting long-standing disrespectful marital relationship. They also had a dualistic perspective on parent-child relationship, accepting parental responsibility for children and even grand-children but maintaining low expectations for children. What they needed was age appropriate opportunities for work or leisure and better community services and facilities. These results showed that the middle-aged was concerned experiencing unprecedented family situations. They needed family life education and services in order to adapt to the Centenarian society. Since family policy has viewed this age group out of service target, programs and services have been underdeveloped for this group. Expecting a Centenarian society however, we need to expand the boundary of family policy and take a new perspective. We need to develop and implement marital education programs, community-based self-care services, and age-appropriated opportunities for work, leisure, and social relations.

• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.11 s.158
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• pp.1626-1635
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• 2006

The purpose of this study was to search for comfortable protective clothing for waste incinerator workers. The experimental protective clothing came in two types: one whose outer side made use of polypropylene film, and the inner side, a non-woven rayon fabric processed with charcoal with a 10% density (CF): and one whose outer side made use of polyolefine and polyamide films, and the inner side, a non-woven polypropylene fabric (NNCF). Experiments were conducted on five healthy adult women whose average age was 21. These experiments were conducted at a climate chamber, in which the temperature and relative humidity were set below $28{\pm}1^{\circ}C\;and\;50{\pm}10%$, respectively. Measured were the rectal temperature, the skin temperature, the sweating rate, the weight loss, the heart rate, the blood pressure, the temperature, and the relative humidity of a microclimate and subjective sensation. These were measured within a period of 60 min, consisting of a 20-min stable period, a 20-min exercise period (walking exercise for 2 miles/hr on a treadmill), and a 20-min recovery period. Through this experiment, the differences between the human body's physiological reactions to CF and NNCF clothing, and the human body's comfort levels when wearing these, were determined.

• The Journal of Korean Physical Therapy
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• v.18 no.5
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• pp.13-23
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• 2006

Purpose: This study was to investigate two-point discrimination(TPD) and related factors in rural community residents. Methods: The sample consisted of 68 people who have been living in rural community in June 2005 studied. TPD was measured from the tips of the thumb, index, middle, ring, and little finger of each hand with the TPD esthesiometer. The research was designed to be a cross-sectional measured study. SAS statistical software was used for the analysis. The characteristics of the study sample were described by mean and standard deviation(SD) for continuous variables and by frequency and percentage for categorical variables. The Student's t-test and analysis of variance (ANOVA) were used to compare TPD in gender and diabetes mellitus. A Pearson's correlation analysis was conducted for relationship among values of 5 fingers. Multiple regression analysis was performed to determine the factors associated with TPD. Results: A total of 68 residents were measured, their average $age{\pm}SD$ was $54.2{\pm}23.2$ years(range: $12{\sim}88\;yr$). The mean TPD for 5 fingers tips in dominated side was 4.76mm(thumb 3.98mm, index 4.22mm, middle 4.79mm, ring 5.17mm, and little finger 5.65mm, respectively). 4.91mm for thumb finger of women was significantly higher than 3.54mm that of men's in difference between gender(p=.01l4), also women(4.39mm) was higher than men(3.71mm) in median nerver area(p=.0318). There was a statistically significant difference in age(p=.0022), which were under age of 20(3.36mm), 30 years(3.61mm), 40 years(5.38mm), 50 years(4.84mm), 60 years(5.28mm), 70 years(5.18mm), and over age of 80(5.29mm). Factors related to TPD of fiver fingers by multiple regressions were gender(${\beta}=.03$, p=.0001). Conclusions: Taken together, gender and age were significantly associated with TPD. We recommend that further research should measure TPD by using larger sample sizes and more sensitive measurement instruments.