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Variation of Fatty Acid Composition in Soybean'Pungsannamul' Mutation Population from EMS Treatment (EMS 처리에 의한 풍산나물 콩 돌연변이 집단에서 지방산 조성 변이)

  • Chae, Jong-Hyun;Dhakal, Krishna Hari;Asekova, Sovetgul;Song, Jong Tae;Lee, Jeong-Dong
    • Current Research on Agriculture and Life Sciences
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    • v.31 no.1
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    • pp.45-50
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    • 2013
  • Mutagenesis is used to study gene function and obtain new genetic resources for plant breeding. Soybean is an important oil crop in the world. Thus, to find new genetic resources, a mutation population was developed from the soybean cultivar Pungsannamul using 0.3% ethyl methane sulfonate. The variation of fatty acids was then evaluated among 892 M4 generation mutant lines selected from 3,774 mutant lines. While the wild type Pungsannamul showed 11.6, 3.4, 23.8, 53.3, and 7.8% for palmitic, stearic, oleic, linoleic and linolenic acid, respectively. the fatty acid variations in the mutant lines ranged from 7.4 to 19.7%, 2.2 to 13.0%, 14.7 to 49.0%, 31.8 to 63.9%, and 3.9 to 15.9% with an average of 10.8, 3.8, 25.3, 52.0, and 8.1% for palmitic, stearic, oleic, linoleic and linolenic acid, respectively. Thus, two mutation lines with higher plamitic acid, PE1542 (17.1%) and PE3058 (17.0%), one line with lower stearic acid, PE2166 (1.9%), one line with higher stearic acid, PE977 (12.7%), two lines with higher oleic acid, PE450 (44.4%) and PE2742 (47.7%), and two lines with lower linolenic acid, PE594 (4.6%) and PE1690 (3.7%), were selected from this study. The newly selected fatty acid variants will be good genetic sources for gene function analyses and breeding soybean varieties with altered fatty acids for various industrial and human food applications.

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Effects of Bosaengtang Administration in Pregnant Rats and Fetuses (보생탕이 랫드의 모체와 태자에 미치는 영향에 대한 연구)

  • Kim, Chang-Seok;Lee, Sun-Dong;Kim, Pan-Gyi;Lee, Jang-Woo;Park, Hae-Mo
    • Journal of Society of Preventive Korean Medicine
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    • v.9 no.2
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    • pp.59-75
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    • 2005
  • The experiments were undertaken to evaluate the effects of Bosaengtang in pregnant rats and fetuses. Female Sprague-Dawley rats were orally administered with Bosaengtang at the dose of 5mg/kg/day for 20 days. Pregnant rats were sacrificed at the 20th day of gestation, and observed internal and reproductive organs. Fetuses were randomly selected and fixed in 95% ethanol. Fetuses were stained with alcian blue and alizarin red S, and observed skeletal malformations. The results obtained were as follows : Bosaengtang administered group showed higher maternal body weight than the control group, but both groups showed increase in weight. Bosaengtang administered group showed lower than the control group, and higher liver and kidney weight than the control group, but the differences were minimal. There were no significant changes between the control and treated group in blood chemistry values and hematological values but all the groups were within in normal ranges. There were no significant changes in the number of corpus luteum, implantation, live fetus and implantation rate, delivery rate, late resorption rate, sex ratio, but Bosaengtang administered group showed higher early resorption rate than control group. comparing the control and Bosaengtang group, neonatal body weight and the number of fetuses were increased in Bosaengtang group. The fetuses of dams treated with Oriental medicine didn't showed external malformation. Vertebral and sternal variations were observed in Bosaengtang group, but the differences were not apparent compared to the control group. The number of ribs, cervical, thoracic and lumbar vertebrae were normal. The number of sacral was similar and the number of caudal was increased. Fetuses showed significant difference in the number of caudal vertebrae. (P<0.01) From these results, we can carefully conclude that Bosaengtang showed beneficial effects on maternal body weight, early resorption rate, number of live fetus. There were no significant changes in organ weight, hematoscopy, reproduction organs. External malformation wasn't visible. Skeletal variations were showed in vertebrae and sternum but compared to the control group, these variations weren't much different.

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Prospective for Successful IT in Agriculture (일본 농업분야 정보기술활용 성공사례와 전망)

  • Seishi Ninomiya;Byong-Lyol Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.6 no.2
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    • pp.107-117
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    • 2004
  • If doubtlessly contributes much to agriculture and rural development. The roles can be summarized as; 1. to activate rural areas and to provide more comfortable and safe rural life with equivalent services to those in urban areas, facilitating distance education, tole-medicine, remote public services, remote entertainment etc. 2. To initiate new agricultural and rural business such as e-commerce, real estate business for satellite officies, rural tourism and virtual corporation of small-scale farms. 3. To support policy-making and evaluation on optimal farm production, disaster management, effective agro-environmental resource management etc., providing tools such as GIS. 4. To improve farm management and farming technologies by efficient farm management, risk management, effective information or knowledge transfer etc., realizing competitive and sustainable farming with safe products. 5. To provide systems and tools to secure food traceability and reliability that has been an emerging issue concerning farm products since serious contamination such as BSE and chicken flu was detected. 6. To take an important and key role for industrialization of farming or lam business enterprise, combining the above roles.

Studies on the Mechanism of Varietal Resistance of Rice to the Brown Planthopper(Nilaparvata lugens $ST{\AA}L$ (벼멸구에 대한 수도품종(水稻品種)의 저항성기작(抵抗性機作)에 관(關)한 연구(硏究))

  • Kim, J.W.;Choi, S.Y.;Park, J.S.
    • Korean journal of applied entomology
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    • v.24 no.2 s.63
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    • pp.51-60
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    • 1985
  • This paper was performed to study the nature of varietal resistance of some Korean-new rice cultivars to the brown planthopper (BPH), Nilaparvata lugens ($ST{\AA}L$). The rice cultivars tested were Cheongcheong, Gaya, Hangangchal, Samgang, Nampoong and Yeongpoong which have been reported as having resistant genes for the BPH. The check varieties were Jinheung, Sangpoong and Chucheong for susceptible and IR-36 for resistant. The factors studied were referred to the seedling responses, preference in feeding and oviposition of BPH, antibiosis (nymphal development, adult emergence and sex ratio, adult body weight, population build-up, feeding amount and amylase activity), and chemical composition (inorganic components, chlorophyll contents, cell wall components, amino acids and esterase isozymes) of leaf- sheath and/or roots of rice plants. In conclusion, the natures of varietal resistance of rice cultivars to the BPH were not only correlated with the resistant reaction of rice plant, but also they were related with the non preference in feeding and oviposition and those resistant cultivars had the antibiotic effects to the insects. Their antibiotic effects of rice cultivars to the BPH would be related with some of the chemical components of rice plants, such as the contents of magnesium oxide (MgO), and chlorophyll and the different esterase isozymes.

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Adult Longevity and Life table analysis of Striped fruit fly, Bactrocera scutellata (Hendel) (Diptera:Tephritidae) (호박꽃과실파리 성충의 수명과 생명표분석)

  • Jeon, Sung-Wook;Kang, Teak-Jun;Cho, Myoung-Rae;Kim, Kwang-Ho;Lee, Sang Guei;Kim, Ji Soo;Park, Hae Woong
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.485-488
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    • 2012
  • The life table of the Striped Fruit Fly, Bactrocera scutellata, was analyzed by using adult longevity and fecundity of B. scutellata at seven constant temperatures (15, 18, 21, 24, 27, 30, $33{\pm}1.0^{\circ}C$) with 65% RH and 16L:8D in the laboratory. The highest values of longevity were observed at $21^{\circ}C$ with the values of 138.0 days for male and 131.2 days for female, respectively. Females of B. scutellata did not oviposit under $18^{\circ}C$ and larvae of B. scutellata could not survive to adulthood over $33^{\circ}C$. The highest value of total fecundity was observed at $24^{\circ}C$ (111.4 eggs) and daily fecundity per female was observed at approximately 1.0. The parameters of the life table were calculated by using the data from eggs to adults of B. scutellata at 4 different temperatures. Net reproduction rate ($R_o$) was highest at $21^{\circ}C$ (52.27). The intrinsic rate of increase ($r_m$) and the finite rate of increase per day (${\lambda}$) were highest at $27^{\circ}C$ (0.07 and 1.07), respectively. The doubling time ($D_t$) was shortest at $27^{\circ}C$ (with 10.02). The mean generation time (T) was shortest at $27^{\circ}C$(50.39).

Relationships between Dietary Variety and Activities of Daily Life in Elderly People Living in Rural Areas of Chungnam Province (충남 일부 농촌지역 노인들의 식품섭취 다양성과 일상생활기능과의 관련성)

  • Chi, Kyung-Hee;Cho, Young-Chae
    • Journal of agricultural medicine and community health
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    • v.30 no.1
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    • pp.75-88
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    • 2005
  • Objectives: This survey was intended to provide basic data which can be available as a baseline in the set up of dietary guidelines for assuring community-based self-support of the rural elderly, through investigation of the relationship of the various dietary consumptions with their ADL and IADL. Methods: The study subjects, 439 rural residents(male: 196, female:243) aged over 65 in Kumsan Kun and Chongyang Kun, Chungchongnamdo Province were interviewed, in June of 2004, about their sociodemographic characteristics, daily life styles, the variety of dietary consumption, ADL and IADL with the following major findings: Results: In terms of the scores' distribution to show variety of food consumption among all subjects, 68.3% got 1~3 points, 23.2% 4~6 points, and 8.4% 7~10 points with a decreasing proportion of subjects in higher points. In terms of their functional status, normal-range groups showed 93.2% of ADL and 72.9% of IADL whereas, impaired ADL group 6.8% of ADL and 27.1% of IADL, respectively. Concerning the relation of ADL and IADL with the variety of their consumed food, the greater scores for food variety was associated with the significantly higher proportion of normal ADL group and the lower proportion of impaired ADL group. Multiple logistic regression analysis with ADL and IADL as dependent variables, and food variety scores as explanatory variables, the relative risk of impaired-ADL group was 0.84 in the food variety group of 4~6 points, 0.63 in 7~10 points with no statistical significance. The relative risk of impaired- IADL group was 0.52(p<0.01) in the food variety group of 4~6 points, 0.41(p<0.05) in 7~10 points with statistical significance. Conclusions: These study results suggest that the lower dietary variety, the lower functional capacity of daily living, and the variety of dietary is associated with the functional capacity of daily living in rural elderly.

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Preparation of $SrTiO_3$ Thin Film by RF Magnetron Sputtering and Its Dielectric Properties (RF 마그네트론 스퍼터링법에 의한 $SrTiO_3$박막제조와 유전특성)

  • Kim, Byeong-Gu;Son, Bong-Gyun;Choe, Seung-Cheol
    • Korean Journal of Materials Research
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    • v.5 no.6
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    • pp.754-762
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    • 1995
  • Strontium titanate(SrTiO$_3$) thin film was prepared on Si substrates by RF magnetron sputtering for a high capacitance density required for the next generation of LSTs. The optimum deposition conditions for SrTiO$_3$thin film were investigated by controlling the deposition parameters. The crystallinity of films and the interface reactions between SrTO$_3$film and Si substrate were characterized by XRD and AES respectively. High quality films were obtained by using the mixed gas of Ar and $O_2$for sputtering. The films were deposited at various bias voltages to obtain the optimum conditions for a high quality file. The best crystallinity was obtained at film thickness of 300nm with the sputtering gas of Ar+20% $O_2$and the bias voltage of 100V. The barrier layer of Pt(100nm)/Ti(50nm) was very effective in avoiding the formation of SiO$_2$layer at the interface between SrTiO$_3$film and Si substrate. The capacitor with Au/SrTiO$_3$/Pt/Ti/SiO$_2$/Si structure was prepared to measure the electric and the dielectric properties. The highest capacitance and the lowest leakage current density were obtained by annealing at $600^{\circ}C$ for 2hrs. The typical specific capacitance was 6.4fF/$\textrm{cm}^2$, the relative dielectric constant was 217, and the leakage current density was about 2.0$\times$10$^{-8}$ A/$\textrm{cm}^2$ at the SrTiO$_3$film with the thickness of 300nm.

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The Effects of gl, gh and wx Gene on the Grain Yield and Yield Components of Rice Plant (수도의 gl, gh 및 wx유전자가 수도수량 및 수량구성요소에 미치는 영향)

  • 김현구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.1
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    • pp.83-109
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    • 1977
  • Isogenic lines for glabrous leaf (gl), hull color(gh) and glutinous endosperm (wx) were used to clarify the effects of the gl, gh and wx gene on the grain yield and yield components in rice plant. Eight genotypes: glgl ghgh wxwx, glgl ghgh WxWx, glgl GH-wxwx, glgl GH-WxWx, GL-ghgh wxwx, GL-ghgh WxWx, GL-GH-wxwx and GL-GH-WxWx were evalutated in the backcrossed $F_2$ generation. Significant effects were measured for gl genotypes on heading date, gh genotypes on grain fertility, and wxwx genotypes on number of panicles per hill and 1, 000 grain weight. The grain yield of straw hull and non glutinous lines were higher than gold hull and glutinous lines. The interaction of three genes, gl, gh and wx was significant for heading date, but not significant in other characters. The average value of isogenic lines which were combined with two recessive genes and one dominant gene were lower and the C.V. value was higher than the isogenic lines which were combined with two dominant genes and one recessive gene in all characters except plant height and number of grains per plant. The average value of isogenic line combined with three recessive genes was lower and the C.V. was higher than the isogenic line combined three dominant genes in all characters measured.

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Susceptibility tests of oral antibiotics including cefixime against Escherichia coli, isolated from pediatric patients with community acquired urinary tract infections (소아 원외 요로감염 환아에서 분리된 E. coli에 대한 cefixime을 포함한 경구 항생제의 감수성 연구)

  • Lee, Soo Young;Lee, Jung Hyun;Kim, Jong Hyun;Hur, Jae Kyun;Kim, Sun Mi;Ma, Sang Hyuk;Kang, Jin Han
    • Clinical and Experimental Pediatrics
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    • v.49 no.7
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    • pp.777-783
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    • 2006
  • Purpose : Urinary tract infection(UTI) is one of the most frequent infections in children. E. coli is the most frequent etiological micropathogen in pediatric community UTI, and E. coli has developed resistance to many antibiotics, highlighting the need for regular surveys of this organism resistant patterns in the community. The aim of this study was to determine the oral antibiotic susceptibility patterns of E. coli, isolated from pediatric patients with uncomplicated community acquired UTI. Methods : E. coli isolates, obtained from pediatric patients with uncomplicated community acquired UTI between October in 2004 to September in 2005. And minimal inhibitory concentrations(MICs) of oral aminopenicillins and beta-lactamase inhibnitors(ampicillin, amoxacillin, ampicillin-sulbactam), oral cephalosporins(cefaclor, cefixime) and sulfa drug(trimethoprime-sulfamethoxazole) were performed according to the National Committee for Clinical Laboratory Standards(NCCLS) guide line. Results : Total 211 organisms were isolated from pediatric out-patients with community UTI. E. coli was the most common organism(89 percent), followed by E. fecalis, Proteus species, S. aureus, M. morganii, and P. aeruginosa. The resistant rates of aminopenicillins and beta-lactamase inhibitors, cefaclor and sulfa drug to E. coli were very high. But, the resistant rate of cefixime was markedly low, and ESBL strains were isolated with small rates. Conclusion : Our study results suggest that aminopenicillins, cefaclor and sulfa drug may not be useful as first line empirical antibiotics to treat pediatric patients with community UTI in Korea. But, 3rd generation cephalosporin such as cefixime can be used as effective second line antibiotics after primary treatment failure, also may be useful as an empirical first line antibiotic. Finally, we conclude that a continuous surveillance study to monitor susceptibility patterns of E. coli in community UTI will be needed for the standard guide lines of empirical oral antibiotic treatment.

Life Table Analysis of the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae) on Rice of Resistant Cultivars (벼멸구 저항성 품종벼에서 벼멸구의 생명표 분석)

  • Choi, Nak Jung;Jeong, In-Hong;Kwon, Deok Ho;Choi, Man-Young;Baik, Chai-Hun
    • Korean Journal of Environmental Biology
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    • v.35 no.4
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    • pp.526-532
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    • 2017
  • Development, survival, and reproduction of brown planthopper (BPH) Nilaparvata lugens $St{\aa}l$ (Hemiptera: Delphacidae), were studied in laboratory at $25{\pm}2^{\circ}C$, $65{\pm}5%$ RH and a 16L : 8D hours photoperiodism on five rice cultivars of: Dongjin 1ho, Chungchungbyeo, Jangseongbyeo, Chinnongbyeo and Jungmo 1045. BPH nymphs successfully survived on all rice cultivars, although survival rate was lowest on Jangseongbyeo (36.0%). Developmental time of immature stages ranged from $11.7{\pm}0.59d$ on Jungmo 1045 to $12.8{\pm}0.59d$ on Chinnongbyeo. Reproductive period and female longevity were longest on Dongjin 1ho, Chinnongbyeo and Jungmo 1045 while highest fecundity of N. lugens being observed on these three rice cultivars. Highest and lowest net reproductive rates were calculated on rice cultivars, Jungmo 1045 and Jangseongbyeo, respectively. Mean generation time was the longest on rice cultivar Dongjin 1ho. Respective descending order of intrinsic rates of population increase were on Jungmo 1045, Chinnongbyeo, Dongjin 1ho, Chungchungbyeo and Jangseongbyeo. These population parameters showed that N. lugens can successfully survive and reproduce on Chinnongbyeo and Jungmo 1045.