• Title/Summary/Keyword: 5'-Fluoro-orotic acid

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A Positive Selection for Pyrimidine Auxotrophic Mutants from Basidiospores of Pleurotus sajor-caju Using 5'-Fluoro-orotic acid (5'-Fluoro-orotic acid를 이용한 여름느타리버섯의 pyrimidine 영양요구성 균주의 positive 선발)

  • Kim, Beom-Gi;Park, Soo-Chul;Jeong, Mi-Jeong;Yoo, Young-Bok;Ryu, Jin-Chang;Kwon, Suk-Tae
    • The Korean Journal of Mycology
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    • v.25 no.1 s.80
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    • pp.26-29
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    • 1997

  • Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5'-fluoro-orotic acid (5'-FOA) whereas pyrimidine auxotrophs grew normally. After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5'-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.

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