• 한국수소및신에너지학회논문집
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• 제22권5호
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• pp.625-633
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• 2011

Characteristics of a commercial metal hydride (MH) hydrogen supply system have been investigated and an operating strategy was developed based on the experimental data. As a prior step, charging/discharging capacity, thermal properties such as heat capacity, heat of reaction of MH system were experimentally measured. And then P-C-T data for various operating conditions were collected and a correlation between P, C and T predicting the behavior of MH was derived. Based on the basic experimental data, an operating strategy of MH system was developed, in which the hot water temperature supplied into the water jacket of MH was controlled depending on the pressure of MH, thereby the pressure of MH could be maintained at a suitable range. By adjusting the temperature of hot water from $40^{\circ}C$ to $60^{\circ}C$, the maximum discharging capacity of hydrogen could be increased by 4.7%, and consequently more stable hydrogen supply and longer operation time of fuel cell system could be achieved.

• 국제지역연구
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• 제13권2호
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• pp.770-788
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• 2009

본 연구는 여수세계박람회의 관람객 유치 활성화를 위해 마케팅의 핵심전략이라고 할 수 있는 STP를 엑스포라는 국제적 이벤트에 적용하고, 아울러 표적시장으로 선정된 집단들을 위해 차별화된 마케팅믹스를 제시하는 데 주요 목적이 있다. 엑스포의 경우, 마케팅믹스를 제품에서의 4P나 서비스에서의 3P와 정확하게 연결할 수는 없지만, 엑스포와 관련하여 다양한 상품 및 프로그램의 개발, 가격의 차별적 책정, 차별적 촉진활동, 접근수단의 효율화 등 제품과 서비스를 응용한 마케팅믹스를 개발함으로써 엑스포 마케팅에 효과적으로 활용할 수 있다. 본 연구는 예상 관람객을 세분화하기 위해 로지스틱회귀분석을 실시하였다. 분석결과, 관람객을 12개의 집단으로 세분화하였으며, 이들 가운데 교육 목적의 국내학생 집단, 비즈니스 목적의 국내전문직 집단, 교육 목적의 외국학생 집단, 비즈니스 목적의 외국전문직 집단, 일반관광 목적의 국내일반인 집단, 일반관광 목적의 외국일반인 집단 등 6개의 집단을 표적집단으로 선정하였다. 그리고 각각의 표적집단에 맞게 차별화된 마케팅믹스를 제시하였다. 특히 여수세계박람회의 차별적 특성을 체계적으로 분석한 후, 이들 차별점을 이용한 포지셔닝을 마케팅믹스와 연계함으로써 효율적인 엑스포 마케팅전략을 수립하고자 하였다.

• Molecules and Cells
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• 제45권10호
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• pp.718-728
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• 2022

Splicing factor B subunit 4 (SF3B4), a component of the U2-pre-mRNA spliceosomal complex, contributes to tumorigenesis in several types of tumors. However, the oncogenic potential of SF3B4 in lung cancer has not yet been determined. The in vivo expression profiles of SF3B4 in non-small cell lung cancer (NSCLC) from publicly available data revealed a significant increase in SF3B4 expression in tumor tissues compared to that in normal tissues. The impact of SF3B4 deletion on the growth of NSCLC cells was determined using a siRNA strategy in A549 lung adenocarcinoma cells. SF3B4 silencing resulted in marked retardation of the A549 cell proliferation, accompanied by the accumulation of cells at the G0/G1 phase and increased expression of p27, p21, and p53. Double knockdown of SF3B4 and p53 resulted in the restoration of p21 expression and partial recovery of cell proliferation, indicating that the p53/p21 axis is involved, at least in part, in the SF3B4-mediated regulation of A549 cell proliferation. We also provided ubiquitination factor E4B (UBE4B) is essential for p53 accumulation after SF3B4 depletion based on followings. First, co-immunoprecipitation showed that SF3B4 interacts with UBE4B. Furthermore, UBE4B levels were decreased by SF3B4 depletion. UBE4B depletion, in turn, reproduced the outcome of SF3B4 depletion, including reduction of polyubiquitinated p53 levels, subsequent induction of p53/p21 and p27, and proliferation retardation. Collectively, our findings indicate the important role of SF3B4 in the regulation of A549 cell proliferation through the UBE4B/p53/p21 axis and p27, implicating the therapeutic strategies for NSCLC targeting SF3B4 and UBE4B.

• Journal of Ginseng Research
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• 제46권2호
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• pp.248-254
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• 2022

Background: Zinc homeostasis is essential for human health and is regulated by several zinc transporters including ZIP and ZnT. ZIP4 is expressed in the small intestine and is important for zinc absorption from the diet. We investigated in the present study the effects of Panax ginseng (P. ginseng) extract on modulating Zip4 expression and cellular zinc levels in mouse Hepa cells. Methods: Hepa cells were transfected with a luciferase reporter plasmid that contains metal-responsive elements, incubated with P. ginseng extract, and luciferase activity was measured. Using ⁶⁵ZnCl₂, zinc uptake in P. ginseng-treated cells was measured. The expression of Zip4 mRNA and protein in Hepa cells was also investigated. Finally, using a luciferase reporter assay system, the effects of several ginsenosides were monitored. Results: The luciferase activity in cells incubated with P. ginseng extract was significantly higher than that of control cells cultured in normal medium. Hepa cells treated with P. ginseng extract exhibited higher zinc uptake. P. ginseng extract induced Zip4 mRNA expression, which resulted in an enhancement of Zip4 protein expression. Furthermore, some ginsenosides, such as ginsenoside Rc and Re, enhanced luciferase activity driven by intracellular zinc levels. Conclusion: P. ginseng extract induced Zip4 expression at the mRNA and protein level and resulted in higher zinc uptake in Hepa cells. Some ginsenosides facilitated zinc influx. On the basis of these results, we suggest a novel effect of P. ginseng on Zip4-mediated zinc influx, which may provide a new strategy for preventing zinc deficiency.

• Nutrition Research and Practice
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• 제15권5호
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• pp.639-654
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• 2021

BACKGROUND/OBJECTIVES: Many adolescents in developing countries skip breakfast. Innovative nutrition education (NE) strategies are needed to enhance knowledge and skills related to the breakfasts of adolescents in a low socioeconomic setting. The objective was to evaluate short- and long-term effects of a multi-strategy, school-based NE intervention on adolescents' breakfast-related personal influences and behaviors. SUBJECTS/METHODS: An intervention study with a cluster randomized controlled trial design was conducted in 4 senior high schools in Makassar, Indonesia. The multi-strategy NE intervention was delivered for 3 months. Data were collected using a self-administered questionnaire and a 3-day breakfast recall (face-to-face interview). Wilcoxon, McNemar, and Mann-Whitney tests were used to determine intra- and intergroup differences. RESULTS: Unlike knowledge, improvement was observed in attitude and self-efficacy scores in the intervention groups (IGs) (P < 0.01); however, no significant changes were observed in the control group (CG). More students showed improved motivation in the IG than in the CG (P > 0.05). Changes in breakfast frequency and macronutrient intake from breakfast were greater in the IG than in the CG (P < 0.05). CONCLUSIONS: A multi-strategy NE intervention is effective in producing positive changes in breakfast-related attitude, self-efficacy, and motivation of adolescents from a low-middle socioeconomic setting. The intervention improved breakfast frequency and nutrient intake. This intervention has promise for sustaining the observed changes over the long-term.

• 보건교육건강증진학회지
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• 제30권4호
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• pp.125-134
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• 2013

Objectives: The aim of this study is to develop the strategy of promoting participation on physical activity programs among the disabled through analysis of satisfaction on the Welfare Center for the Disabled-based program. Methods: The subjects were 151 disabled people who had physical disability or brain disorder. They had participated on the physical activity programs which were opened in 13 welfare centers for the disabled of Daegu and Gyeongbuk. The data were collected by the survey for 2 weeks in May, 2011. Results: There were no differences in the satisfaction on physical activity programs by general characteristics and health status. As for cognitive factors, it were significantly different on the satisfaction by the importance of physical activity and the fitness maintenance factor (p<0.05, p<0.01). Significant differences were found in the satisfaction by the participating attitude and the participation motives, such as goal-oriented and activity-oriented motives. It were significantly different on the satisfaction by the duration, frequency per week, time per once, and components in terms of the physical activity program. Conclusions: To revitalize the physical activity-for-all project for the disabled, it needs to develop the strategy and to enhance the health education to improve their health status.

• 한국간호교육학회지
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• 제18권2호
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• pp.239-247
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• 2012

Purpose: This study is to assess the level of student perceptions, academic achievement, satisfaction, and their relationships in a medical-surgical nursing course using team-based learning (TBL). Method: Four-hour TBL sessions were given in a structured three-phase sequence in a cohort of 261 second year nursing students. Results: Mean perceptions of TBL was $6.64{\pm}5.11$, and $8.30{\pm}4.11$ for perceptions of teamwork. On a 7-point scale, the mean satisfaction score was $4.85{\pm}1.41$, and 64.0% of nursing students were satisfied with TBL compared to lecture. Group readiness assurance test score was significantly higher than individual readiness assurance test score (t=-16.76, p<.001). Perceptions of TBL (F=1.40, p=.245), perceptions of team work (F=1.55, p=.202) and satisfaction (F=0.81, p=.489) was not different by the level of students' academic achievement on items related TBL. Conclusion: Results indicates that TBL was an effective instructional strategy including favorable perceptions and satisfaction for nursing students. TBL could be an adjunct educational strategy for undergraduate nursing education.

• 한국융합학회논문지
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• 제10권8호
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• pp.27-36
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• 2019

본 연구는 우리나라 50-60대 골관절염 환자에서 우울감 및 자살사고 위험의 관계를 알아보고, 이에 따른 영양소 섭취 실태를 파악하고자 하였다. 제 6기 국민건강영양조사 자료에서 의사로부터 골관절염 진단을 받은 4,112명을 분석하였다. 골관절염이 있는 경우에 골관절염이 없는 경우에 비해 불안 및 우울 수준, 통증 및 불편감 수준, 2주 이상 연속 우울감 경험률, 1년간 자살 생각률이 유의적으로 높았다(p<0.05). 일일 평균 영양소 섭취 차이에서 단백질, 지방, n-3계 지방산, n-6계 지방산, 칼슘, 인, 티아민, 리보플라빈 및 나이아신 섭취량이 공통적으로 정상군에서 각각 골관절염과 우울감 및 자살사고군에 비해 유의하게 높은 것으로 나타났다(p<0.05). 따라서 골관절염 환자의 정신적 건강과 추후 환자 건강 관리 향상을 위한 융합적 전략의 방향을 제시하고자 하였다.

• 재활간호학회지
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• 제4권1호
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• pp.46-57
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• 2001

This study was done to investigate of instrumental activities of daily living(IADL) and quality of life and their relationships between the elderly. Data were collected from 150 elderly home residents. The data were collected from December 1st to December 20th, 2000. Structured questionnaires developed by Lawton & Brody and by Ro were adopted to measure IADL and Quality of life. Data were analyzed for percentage, means, t-test, ANOVA and Parson correlation coefficients using the SPSS program. The results were as follows: 1. The average score of the level of IADL was $20.68{\pm}2.98$ ($M{\pm}SD$) with a range of 7-26. 2. The average score of the level of Quality of life was 2. $96{\pm}0.25$ ($M{\pm}SD$). In the average score of each lower area, highest score was 3.22 for neighbor relationship and family relationship and then 3.00 for self-esteem, 2.95 for economic status, 2.86 for emotional status, and 2.85, lowest score, for physical condition and function. 3. Comparing IADL and Quality of life with general characteristics, IADL showed that there were significant difference in age(t=2.927, p=0.036) and separated children contact frequency(t=2.482, p=0.046), while Quality of life showed that there were significant difference in spouse existence(t=2.334, p=0.021), religion(t=4.089, p=0.008), family style(t=3.285, p=0.040), children number living together(t=5.332, p=0.006), communication with separated children frequency(t=4.129, p=0.003), and separated children contact frequency(t=3.908, p=0.005). 4. There was also significant positive correlation between IADL and Quality of life. The above results show that neighbor relationship and family relationship which have greatly an influence on IADL and Quality of life are very important. The elderly should be helped to satisfy their basic desire and show their potential living together with the younger generation interdependently without being isolated from family and society. Therefore, the nursing strategy that enhance IADL and Quality of life are needed and the nursing strategy that can improve IADL and Quality of life of Elderly Home Residents should be developed.

• Journal of Korean Neurosurgical Society
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• 제59권4호
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• pp.379-384
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• 2016

Objective : Mechanical thrombectomy (MT) for acute intracranial internal carotid artery (ICA) occlusion is often complicated by difficult revascularization and non-involved territory embolization possibly related with larger clot-burden. This study aims to evaluate the efficacy of proximal aspiration thrombectomy (PAT) using a balloon-tipped guide catheter for clot-burden reduction in such cases with period-to-period analysis (period 1 : standard MT without PAT; period 2 : PAT first, then standard MT for the remaining occlusion). Methods : Eighty-six patients who underwent MT for acute intracranial ICA occlusion were included in this analysis from the prospectively maintained stroke registry (33 patients in period 1 and 53 in period 2). In period 2, 'responder' was defined as a case where some amount of clot was retrieved by PAT and the following angiography showed partial or full recanalization. Results : Fifteen of fifty-three patients in period 2 (28.3%) were 'responders' to PAT. There was a significantly higher incidence of atrial fibrillation in the 'responder' subgroup. Period 2 showed a significantly shorter puncture-to-reperfusion time (94.5 minutes vs. 56.0 minutes; p=0.002), a significantly higher Thrombolysis in Cerebral Infarction of 2b-3 reperfusion (45.5% vs. 73.6%; p=0.009), but only a trend for better 3-month favorable outcome (mRS 0-2; 36.4% vs. 54.7%; p=0.097). There was no increase in the incidence of procedure-related complications or intracranial hemorrhage in period 2. Conclusion : A strategy of PAT before standard MT may result in shorter puncture-to-reperfusion time and better angiographic outcome than a strategy of standard MT for acute intracranial ICA occlusion.