• Advances in nano research
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• 제12권6호
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• pp.639-652
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• 2022

The prolyl 3-hydroxylase family member 4 (P3H4), is associated with post-translational modification of fibrillar collagens and aberrantly activated in cancer leading to tumor progression. However, its role in clear cell renal cell carcinoma (ccRCC) is still unknown. Here we reported that P3H4 was highly expressed in renal cancer tissues and significantly positive correlated with poor prognosis. Knockdown of P3H4 inhibited the proliferation, migration and metastasis of renal cancer cells in vitro and in vivo, and also, overexpression of it enhanced the oncogenic process. Mechanistically, P3H4 depletion decreased the levels of GDF15-MMP9 axis and repressed its downstream signaling. Further functional studies revealed that inhibition of GDF15 suppressed renal cancer cell growth and GDF15 recombinant human protein (rhGDF15) supplementation effectively rescued the inhibitory effect induced by P3H4 knockdown. Moreover, decreased levels of MMP9 caused by inhibition of P3H4-GDF15 signaling constrained the expression of PD-L1 and suppression of P3H4 accordingly promoted anti-tumor immunity via stimulating the infiltration of CD4+ and CD8+ T cells in syngeneic mice model. Taken together, our findings firstly demonstrated that P3H4 promotes ccRCC progression by activating GDF15-MMP9-PD-L1 axis and targeting P3H4-GDF15-MMP9 signaling pathway can be a novel strategy of controlling ccRCC malignancy.

• 대한간호
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• 제36권4호
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• pp.88-99
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• 1997

Applying effective coping strategies and reducing powerlessness for the chronic pain adjustment of musculoskeletal patients were researched for basic data in the development of nursing intervention. The subjects were 99 musculoskeletal patients with chronic pain. Data was gathered with direct interview using a questionnaire. With the SAS tool. data were analyzed for percentage. Pearson correlation. t-test. and ANOVA according to characteristics of variables. Internal consistency(alpha) coefficients were .91 for coping scales and .71 for powerlessness scales. The results are as follows: 1. Pain duration was mainly 6 - 12 months(52. 5%). 2. Primary pain site was mainly lower limbs(58.6%) and pain severity was a moderate level. 3. The preference of coping strategies was decreased in the order named in resting. seeking social support. exercise or stretch. guarding. and asking for assistance. 4. The longer pain duration. the more the coping strategies of asking assistance used. This was stastically significant(F=3. 35. p<.05). 5. The group with the experience of hospital admission was more powerless than the group without that(F=3. as. p<.01). 6. Pain severity and powerlessness were significantly positively correlated(r=.444. p<.001). Coping strategy applying and powerlessness were significantly negatively correlated(r=-. 288. p<.01). In consequence. the nurse should playa role as the supporter of patient's maximal usage of his resources in pain relief. adjustment, and control. The nurses should also develop for the nursing intervention of physical therapy and educational programs.

• Journal of Periodontal and Implant Science
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• 제37권1호
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• pp.11-21
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• 2007

Heat shock protein (HSP) is one of cellular protein commonly present in major periodontopathogenic bacteria as well as mammalian cells. The protein may play a role in the immunopathogenesis by modulating autoimmune reaction due to its high level of sequence homology between bacteria and human counterpart. Hence, identifying immunodomiant epitope of bacteria HSP that is cross-reactive to periodontopathogenic bacteria with a specificity to human HSP may comprise a critical strategy for development of a periodontal vaccine. The present study was performed to establish clones producing monoclonal antibody reactive to Porphyromonas gingivalis (p. gingivalis) HSP with a specificity to human HSP. 4 different hybridomas were cloned producing monoclonal IgG antibodies to P, gingivalis HSP and evaluated for their reactivity and specificity to other periodontopathogenic bacteria as well as to human HSP. These four monoclonal antibodies reacted with p. gingivalis HSP only with specificities to other bacteria tested and human HSP as well. The antigenic epitopes producing the 4 monoclonal antibody may be potentially developed as vaccine candidates. Further investigations are under way to identify more clones producing monoclonal antibodies reactive to P, gingivalis HSP and to other periodontopathogenic bacteria as well, while maintaining specificities to human counterpart.

• Child Health Nursing Research
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• 제25권2호
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• pp.123-132
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• 2019

Purpose: A hand-washing program using role-playing was developed for preschool children and tested to verify its usefulness. Methods: The participants were 46 preschool children (experimental group, 23; control group, 23). Data were collected from August 7, 2017 to September 20, 2017. The first posttest was carried out on the day after the initial intervention was completed, and the second posttest was performed after a month. Data were analyzed using the $x^2$ test, Fisher exact test, Shapiro-Wilk test, Mann-Whitney test, and Friedman test. Results: In the experimental group, the frequency of practicing hand-washing was significantly higher than in the control group on the next day (z=-4.94, p<.001) and after a month (z=-3.15, p=.002). The accuracy of hand-washing was significantly higher in the experimental group than in the control group on the next day (z=-4.02, p<.001) and after a month (z=-3.86, p<.001). Conclusion: The hand-washing program developed in this study was found to be an effective intervention strategy for improving the frequency and accuracy of hand-washing among preschool children.

• Radiation Oncology Journal
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• 제35권4호
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• pp.332-339
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• 2017

Purpose: This study aimed to evaluate the prognostic effects of lymphovascular invasion (LVI) in triple-negative breast cancer (TNBC) patients who underwent surgical resection. Materials and Methods: A total of 63 non-metastatic TNBC patients who underwent surgical resection were retrospectively investigated from 2007 to 2016 in Inje University Busan Paik Hospital. Pathological tests revealed that 12 patients (19.0%) had LVI. Approximately 61.9% (n = 39) of the patients' samples stained positive for p53. Additional chemotherapy and radiotherapy (RT) were performed in 53 (84.1%) and 47 (74.6%) patients, respectively. Results: The median follow-up period was 39.5 months (range, 5.9 to 123.0 months). The pathological T stage (p = 0.008), N stage (p = 0.014), and p53 positivity (p = 0.044) were associated with LVI. Overall, the 3-year disease-free survival (DFS) rate and overall survival (OS) rate were 85.4% and 90.2%, respectively. Ten patients (15.9%) experienced relapse. LVI (n = 12) was associated with relapses (p = 0.016). p53 positivity was correlated with poor DFS (p = 0.048). Furthermore, LVI was related to poor DFS (p = 0.011) and OS (p = 0.001) and considered as an independent prognostic factor for DFS (p = 0.039). The 3-year DFS of patients with LVI (n = 12) was only 58.3%. Adjuvant RT minimized the negative prognostic effect of LVI on DFS (p = 0.068 [with RT] vs. p = 0.011 [without RT]). Conclusion: LVI was related to the detrimental effects of disease progression and survival of TNBC patients. Thus, a more effective treatment strategy is needed for TNBC patients with LVI.

• KSBB Journal
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• 제14권4호
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• pp.482-489
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• 1999

As host for the production of eucaryotic heterologous proteins, methylotrophic yeast Pichia pastoris and Hansenula polymorpha are the most highly developed of a small group of alternative yeast species chosen for their perceived advantages. This paper describes the method to enhance the recombinant protein productivity with P. pastoris and H. Plymorpha. In these experiments, the effects of methanol induction timing, induction method, pH, culture temperature and kinds of nitrogen sources on foreign protein production were tested with P. pastoris and compared with H. polymorpha.. In addition, optimum methanol concentration as inducer and the effects of carbon sources on AOX1 or MOX promoter repression and secretion efficiency were also studied in both cases.

• 동아시아식생활학회지
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• 제26권2호
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• pp.181-191
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• 2016

This study aimed to investigate current nutrition education and effective plans for nutrition teachers in schools. Data were collected by a face-to-face questionnaire. Surveyed schools were elementary, middle, and high schools at percentages of 32.4%, 35.1%, and 32.4%, respectively. Percentage of nutrition education was above 90%, whereas off-line education was very uncommon in middle (8.3%) and high schools (27.3%) compared with elementary schools (63.6%), (p<0.05). Satisfaction of nutrition education was also very low in middle (7.7%) and high schools (8.3%) when compared with elementary schools (41.7%), (p<0.05). The main reason for this was due to 'work overload of mealing service (44.4%)' and 'insufficient time of students (25.9%)'. Effective education plans were as follows: beginning of education and education scale were 'elementary school (67.6%)' and 'below 20 people (81.1%)', and the most preferred counterplan was 'development of diverse education programs (4.81 score)' > 'systematic education process (4.76 score)' > 'professional nutrition education (4.51 score)'. Most important duty was 'nutrition education and consultation (51.4%)', although it achieved the lowest satisfaction at a score of 2.46. Overall analysis, demand and awareness of nutrition teachers for nutrition education were very high. Therefore, an institutional strategy and environmental improvements are required by setting up curriculum subjects and developing diverse education programs for systematic nutrition education.

• 융합정보논문지
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• 제8권4호
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• pp.55-62
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• 2018

본 연구는 10회의 전학급동료교수학습이 간호대학생의 학업성취와 셀프리더십 및 대인관계능력에 미치는 효과 확인을 위한 원시실험연구로, 수집된 자료를 SPSS/WIN 19.0으로 Mann-Whitney U와 Kruskal-Wallis 검정 결과, 변수의 평균 순위값의 영향요인은 입학동기였다. Wilcoxon Signed Ranked Test 결과, 실험처치 전후 전학급동료교수학습은 학업성취에 유의한 효과가 있었고(p<.001), 셀프리더십의 하위영역 중 인지적 전략과, 대인관계능력의 하위영역 중 관계촉진의 평균순위값에 유의한 차이가 있는 것으로 나타났으며(p=.020; p=.040), 셀프리더십과 대인관계능력 간 양의 상관관계가 있었다(r=.296, p=.041). 본 연구 결과 전학급동료교수학습은 간호대학생들의 학업성취도를 향상시켰고, 셀프리더십과 대인관계능력에 효과가 있는 중재로 볼 수 있으나, 대상자 수를 확대하고, 대조군을 설정한 유사실험연구로 실험결과를 재확인하는 연구를 제언한다.

• 성인간호학회지
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• 제17권2호
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• pp.310-319
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• 2005

Purpose: The purpose of the study is to investigate the degree of fatigue and its related factors in patients with breast cancer. Method: The subjects of this study consisted of 62 breast cancer patients receiving chemotherapy. The Revised Piper Fatigue Scale, Disruption of Usual Activity Scale, Linear Analogue Self Assessment Scale and Self-Care Diary Scale were employed to measure fatigue, disruption of usual activity, mood state and physical symptom respectively. Collected data were analyzed using SPSS-PC to obtain summary statistics for the descriptive analysis, Pearson Correlation, and Stepwise Multiple Regression. Result: The results are as follows. 1. The mean score of the degree of fatigue was 4.82 points indicating moderate level. 2. The mean score of the degree of disruption of usual activity, mood state and physical symptom were 2.66, 5.00, and 4.69 points. 3. Fatigue of the subjects was significantly correlated with disruption of usual activity(r=.517, p<.01), mood state(r=.420, p<.01) and physical symptom(r=.463, p<.01). 4. With the result of stepwise multiple regression, disruption of usual activity, mood state and physical symptom being the three variables which could explain fatigue by 39.1%. The results of this study suggest that comprehensive intervention strategy for fatigue should be developed to maintain quality of life during and following chemotherapy considering these factors.

• 간호행정학회지
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• 제14권1호
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• pp.26-34
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• 2008

Purpose: This Study was performed to measure the moral judgment of nursing students, and to identify an effect of ethics education being provided to nursing students. Method: This study is a kind of nonequivalent control group pretest-posttest using the Korean version of DIT which can measure the level of human's moral development. Result: In experimental group, the posttest scores on 4 stage and P are higher than the pretest of them, but these gaps between posttest and pretest are not statistically significant. The posttest scores of lower group on 4 stage and P are higher than the pretest scores of them, and these gaps are statistically significant in experimental group. The posttest scores of higher group on 4 stage and P are lower than the pretest scores of them, and these gaps are statistically significant in control group. Conclusion: It is concluded that the ethics education being provided to nursing students has an effect in this study. And it is suggested that it may be a good strategy to use the various scores of DIT to verify an effect of ethics education.