• Journal of Ecology and Environment
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• 제42권3호
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• pp.111-119
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• 2018

Background: Juniperus chinensis L. populations are distributed locally on several areas including coastal cliffs which are difficult to access in the central eastern Korea. Wild populations inhabit relatively barren environments such as rocky areas and cliffs, which are very sensitive to even minor environmental disturbances including artificial interventions and natural disturbances, and thus demonstrate great fluctuations in the population size and density. This study aims to analyze the genetic diversity, differentiation, and genetic structure of each population in order to provide useful data required to establish a substantial conservation strategy of J. chinensis. Results: The genetic diversity of J. chinensis at the population level (P = 78.7%, h = 0.282, S.I. = 0.420) was somewhat higher compared with those measured in the same genus, Juniperus. The genetic differentiation degree among nine populations established naturally in central eastern Korea was 11.50% and that among sub-populations within the same area was 5.52%. On the other hand, genetic variation of individuals within the populations was 82.93%. But frequency of the main allele was different among loci. In particular, fixation of allele frequency and occurrence of rare allele in the highly isolated population suggest a likelihood that genetic drift would occur in populations of this plant. As the result of analysis on the genetic structure of nine populations, nearby populations and isolated populations tended to form separate clusters from each other as the hypothetical number of clusters (K) increase. Conclusions: This result implies that if the population size of J. chinensis is reduced due to environmental change and artificial and/or natural disturbances in the future, it could affect negatively on the genetic diversity of the plant species. In order to maintain and conserve genetic diversity of J. chinensis, ecological network, which can help genetic exchange among the local populations, should be prepared, and conservation strategies in situ as well as ex situ are also required with continuous monitoring.

• 한국콘텐츠학회논문지
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• 제19권10호
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• pp.568-580
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• 2019

MaaS의 영향력이 주목받고 있는 가운데, 공유 모빌리티를 중심으로 모빌리티 서비스 시장이 급격히 성장하고 있다. 시장 선점을 위해서 현재 초기 확산단계에 있는 퍼스널 모빌리티 서비스 이용자의 인식과 경험을 파악하고, 향후 확장될 시장의 잠재 소비자들의 특성을 이해하는 것이 중요하다. 따라서 이 연구는 주관성 연구방법인 Q 방법론을 사용하여 퍼스널 모빌리티 이용자 유형을 분류하고 UX(User experience) 전략을 수립하고자 하였다. 제품 및 서비스 특성과 이용자의 개인적 특성 요소들을 중심으로 41개 구성하고, 잠재 사용자와 실사용자를 포함하는 30명의 P샘플을 대상으로 데이터를 수집하였다. QUANL 소프트웨어를 사용하여 수집한 데이터를 분석하였으며, 그 결과 (1)효율적인 이동 우선형(Mobility Efficiency), (2)경계하는 안전추구형(Safety First), (3)합리적인 여가활용형(Rational Utilization), (4)호기심 많은 재미추구형(Sheer Riding Pleasure)의 네 가지 심성 모형이 도출되었다.

• 한국보건간호학회지
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• 제18권2호
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• pp.286-298
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• 2004

The purpose of this study was to survey the general characteristics, lifestyle include health habits, subjective health problems include physical, psychological and overall health complaints that occurring among Korean and Japanese nursing students. Data collected with the self-administered Okayama Medical Index Health Questionnaire(devised Cornell Medical Index) from 466 Korean and 688 Japanese Nursing Students were analyzed. The subjects were 18 to 24 years old in their 1st, 2nd, 3rd and 4th year-class of nursing department. The total O.M.I. score(section A-R) ranged from 1 to 110 with an average of 22.3(SD=11.5) in Korean nursing students and 18.3(SD=10.1) in Japanese nursing students. A statistically significant inverse correlation was observed between Korean nursing student and Japanese nursing students for the overall health complaints. Korean nursing students have more health problems than Japanese nursing students in terms of physical and overall health complaints excluding psychological problems. About $4\%$ of Korean nursing students were current smokers and $64.2\%$ of Korean nursing students were drinker. Korean nursing students in the drinker's group were more likely to eat fatty foods(p<.001), and take worst lifestyle and have more subjective health problems than non drinker's group of Korean nursing students. Korean nursing students were more likely to perform bad health behaviors than Japanese nursing students. The drink habit is one of the biggest problems among the nursing student, and then we have to have the mobilizing for 'Change on Alcohol' in our universities. This conclusion should provide grounds for future studies of strategies for health promotion and of prevention measures for psychological ill-health of nursing students.

• The Korean Journal of Physiology and Pharmacology
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• 제24권4호
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• pp.299-310
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• 2020

Alzheimer's disease (AD) is a multi-faceted neurodegenerative disease. Thus, current therapeutic strategies require multitarget-drug combinations to treat or prevent the disease. At the present time, single drugs have proven to be inadequate in terms of addressing the multifactorial pathology of AD, and multitarget-directed drug design has not been successful. Based on these points of views, it is judged that combinatorial drug therapies that target several pathogenic factors may offer more attractive therapeutic options. Thus, we explored that the combination therapy with lower doses of cilostazol and aripiprazole with add-on donepezil (CAD) might have potential in the pathogenesis of AD. In the present study, we found the superior efficacies of donepezil add-on with combinatorial mixture of cilostazol plus aripiprazole in modulation of expression of AD-relevant genes: Aβ accumulation, GSK-3β, P300, acetylated tau, phosphorylated-tau levels, and activation of α-secretase/ADAM 10 through SIRT1 activation in the N2a Swe cells expressing human APP Swedish mutation (N2a Swe cells). We also assessed that CAD synergistically raised acetylcholine release and choline acetyltransferase (CHAT) expression that were declined by increased β-amyloid level in the activated N2a Swe cells. Consequently, CAD treatment synergistically increased neurite elongation and improved cell viability through activations of PI3K, BDNF, β-catenin and α7-nicotinic cholinergic receptors in neuronal cells in the presence of Aβ_1-42. This work endorses the possibility for efficient treatment of AD by supporting the synergistic therapeutic potential of donepezil add-on therapy in combination with lower doses of cilostazol and aripiprazole.

• Journal of Ginseng Research
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• 제42권3호
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• pp.379-388
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• 2018

Background: Ginsenosides are the main ingredients of Korean Red Ginseng. They have extensively been studied for their beneficial value in neurodegenerative diseases such as Parkinson's disease (PD). However, the multitarget effects of Korean Red Ginseng extract (KRGE) with various components are unclear. Methods: We investigated the multitarget activities of KRGE on neurological dysfunction and neurotoxicity in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. KRGE (37.5 mg/ kg/day, 75 mg/kg/day, or 150 mg/kg/day, per os (p.o.)) was given daily before or after MPTP intoxication. Results: Pretreatment with 150 mg/kg/day KRGE produced the greatest positive effect on motor dysfunction as assessed using rotarod, pole, and nesting tests, and on the survival rate. KRGE displayed a wide therapeutic time window. These effects were related to reductions in the loss of tyrosine hydroxylase-immunoreactive dopaminergic neurons, apoptosis, microglial activation, and activation of inflammatory factors in the substantia nigra pars compacta and/or striatum after MPTP intoxication. In addition, pretreatment with KRGE activated the nuclear factor erythroid 2-related factor 2 pathways and inhibited phosphorylation of the mitogen-activated protein kinases and nuclear factor-kappa B signaling pathways, as well as blocked the alteration of blood-brain barrier integrity. Conclusion: These results suggest that KRGE may effectively reduce MPTP-induced neurotoxicity with a wide therapeutic time window through multitarget effects including antiapoptosis, antiinflammation, antioxidant, and maintenance of blood-brain barrier integrity. KRGE has potential as a multitarget drug or functional food for safe preventive and therapeutic strategies for PD.

• 한국간호교육학회지
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• 제9권2호
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• pp.212-221
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• 2003

The purpose of this study was to evaluate the effects and define the educational strategies of the cyber education offered to students for Bachelor of Science degree program(RN-BSN). The participants in this study were 67 students in two courses, Growth and Development, and Nursing Process. The main components of the cyber classes were the electronic board and on-line discussion board. The study was conducted from March 4, 2001 to July 23, 2001 at Y University in Seoul, Korea. To examine the effects of the cyber education, learning motivation, and learning satisfaction were measured by questionnaires to the students before the initial class and after completing the class. Learning achievement was measured by the grades for each course. The data were analyzed using frequencies, t-test, and paired t-test. The specific results of the course evaluation were compared and analysis was done to examine differences between traditional classroom teaching and cyber teaching. The study results are as follows: 1. There were no significant differences on total scores for learning motivation between pre-cyber education and post-cyber education. However, there were significant decreases in the post test compared to the pre test for the items, ' I'd like to get as high a score as possible compared to other students (t=-2.20, p=.03)' and ' I'm sure to acquire good grades(t=-5.22, p=.00) '. 2. The average score for learning satisfaction using cyber education was high at 3.52. 3. To define learning achievement using cyber education, grades for students taking cyber classes this year were compared to student grades for a class using traditional lassroom teaching last year. The score was significantly higher for classroom students in the 'Growth and Development' course (t=-3.5, p<.001), and the score was significantly higher for the cyber education students in the 'Nursing Process' course (t=4.3, p<.000). 4. The average of post scores on computer competency was significantly higher on six items, general knowledge about computers, data management and data research, ability to communicate using computer, and internet surfing. On the basis of the above findings, this study suggests that cyber education in nursing courses is effective and readily available. However, it is recommended that consideration be given to characteristics of the course when developing cyber education programs for nursing courses.

• 대한치과보철학회지
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• 제45권3호
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• pp.362-374
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• 2007

Statement of problem. The intial stability for osseointegration of implant has been an interesting factor. Especially, in the case of poor bone quality or immediately loaded implant, various strategies have been developed focusing on the surface of materials to improve implant fixation to bone. The microscopic properties of implant surfaces play a major role in the osseous healing of dental implants. Purpose. The aims of this study are to perform a histologic and histomorphometric comparison of the healing characteristics of three different surfaces and the comparison of resonance frequency analysis (RFA) values measured by $Osstell^{TM}$ and perio-test values (PTV) measured by Periotest. Material and methods. A total of 24 screw titanium implants (Dentium Co., Seoul, Korea) with 6mm in length and 3.4mm in diameter, were placed in the mandible of 4 beagle dogs. Implants were divided into three groups following the surface treatment methods: Group I is machined(control group). Group II is anodically oxidized. Group III is coated 500nm in thickness with hydroxyapatite(HA) by ion beam assisted deposition(IBAD) on the anodized oxidization. Bone blocks from 2 dogs were caught after 3 weeks of covered healing and another blocks from 2 dogs after 6 weeks. RFA values and PTV were measured right after insertion and at 3 and 6weeks. Histomorphometric analysis was made with Kappa Image Base System to calculate bone-to-implant contact (BIC) and bone area inside the threads. Pearson's correlation analyses were performed to evaluate the correlation between RFA and PTV, BIC and bone area ratio of three different surfaces at 3 and 6 weeks. Results. 1) In all surface treatment methods, the RFA values decreased and the PTV values increased until 6 weeks in comparison to initial values. 2) At 3 weeks, no significant difference was found from bone-to-implant contact ratio and bone area ratio of three different surface treatment methods(P>0.05). However, at 6 weeks, different surface treatment methods showed significantly different bone-toimplant contact ratio and bone area ratio(P<0.05). 3) In the implants with the IBAD on the anodic oxidization, significant difference was found between the 3 weeks and the 6 weeks bone area ratio(P<0.05). 4) Correlation was found between the RFA values and the bone area ratio at 3 and 6 weeks with significant difference(P<0.05). Conclusions. These results indicate that the implants with the IBAD on the anodic oxidization may have a high influence on the initial stability of implant.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.219-224
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• 2013

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7523-7527
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• 2015

Background: Pediatric acute lymphoblastic leukemia (pALL) patients at King Abdulaziz Medical City represent a pure Saudi Arabian population. ETV6-RUNX1 positive pALL patients have good prognosis as compared to ETV6-RUNX1 negative counterparts. Therefore, frequencies of these two patient groups have a huge consideration in treatment strategies of pALL in a given population. Different geographical locations have been reported to have different frequencies of ETV6-RUNX1 ranging from 10% in Southeast Asia to 30% in Australia. Aim: Therefore, the objective of this study was to establish the ETV6-RUNX1 status of Saudi Arabian pALL patients and its association with clinical parameters and early remission. Materials and Methods: Clinical parameters and ETV6-RUNX1 status (using FISH technique) of pALL patients attending the Pediatric Oncology Clinic, King Abdulaziz Medical City, Riyadh from 2006 to 2011 were studied. Comparisons between ETV6-RUNX1 positive and negative groups were accomplished using chi-square test or Fisher's exact test. All statistical analyses were performed using SAS version 9.2 (SAS Institute, Inc., Cary, NC). Results: Out of 54 patients, 33 were male and 21 were females (ratio 1.57:1). B- and T-cell lineages were found in 47 (87%) and 7 (13%) patients respectively. Only 5 (9.3%) patients were ETV6-RUNX1 positive while 49(80.7%) were ETV6-RUNX1 negative. All ETV6-RUNX1 patients (100%) were of B-cell lineage and 80% (4/5) were in the 3-7 year age group. None of the ETV6-RUNX11 patients had ${\geq}5%$ blasts (no remission) at day 14 as compared with 9% in the ETV6-RUNX1 negative group (Figure 1). Conclusions: Frequency of ETV6-RUNX1 positive patients (less than 10%) in our pALL patients is much lower than reported for most European countries, North America, Australia and Japan while it is in accordance with ETV6-RUNX1 frequencies from Egypt (11.6%), Pakistan (10%), Spain (2%) and India (5-7%). This shows ethnic differences in genetics of pALL as well as higher frequencies of ETV6-RUNX1 positive pALL mostly in more industrialized countries, probably due to some industrial pollutants or westernized lifestyle.

• 한국식품영양과학회지
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• 제29권4호
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• pp.701-711
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• 2000

본 연구는 우리나라의 식생활에 적절한 영양 평 가 방법을 제시하는데 기여하고자 하는 목적으로, 경북지역에 거주하는 성인 남녀 196명을 대상으로 24시간 회상법을 통해 개인별 영양소 섭취상태를 조사한 후 영양상태를 질적, 양 적으로 평가하였다. 본 연구의 결과는 다음과 같다. 경북지역 성인 남녀의 일일 평균 영양소 섭취량은 비교적 양호한 편이나, 칼슘과 비타민 A에서 영양 권장량보다 적은 83%, 77%를 섭취했으며, 칼슘을 제외한 모든 영양소에서 남자의 평균 섭취량이 여자의 평균 섭취량보다 많았다. INQ로 살펴본 영양소 섭취의 질적 평가는 비타민 C가 2.39로 가장 높게 나왔으며, 비타민 A가 0.84, 칼슘이 0.89의 점수를 보여 Ca과 비타민 A는 양과 질 측면에서 우려되는 것으로 조사되었다. 평균 영양소 적정도는 남자가 0.88, 여자가 0.86으로 남녀 간에 차이가 없었다. 영양소 섭취량과 각 용니과의 상관관계에서 남자의 경우에는 영양소 섭취량과 유의 한 관계를 나타내는 요인으로서 지방과 연령 사이에만 유의한 양의 관계가 나타난 반면 여 자의 경우에는 나이, 식사 섭취 횟수는 대부분의 영양소에서 유의적인 양의 상관관계를 보 였다. 남자는 가계 총수입이 높을수록 식사 중의 비타민 B2 영양밀도가 높았으며 여자의 경 우에는 INQ 중에서 인(p<0.050)과 철분(p<0.05)이 연령과 유의한 양의 상관관계를 보였다. MAR은 여자의 경우에 한하여 연령(p<0.05)과 양의 상관관계를, 학력(p<0.01)과 유의적인 음의 상관관계를 보였으며 하루식사횟수(p<0.001)와도 유의한 양의 관계를 나타내었다. 이 상을 종합해 볼 때 경북지역에 거주하는 성인의 경우 남자가 여자에 비해 영양섭취상태가 전반적으로 양호하다고 할 수 있으며 여자 20대의 경우에 영양섭취가 가장 저조하였고 특히 칼슘과 비타민 A의 섭취가 부족하였다. 이연령층은 임신, 출산과 연결되는 영양취약집단이 므로 이들을 대상으로 규칙적인 식사의 중요성과 다양한 식품의 섭취를 홍보하는 영양교육 이 보건소를 통해 강화되어야 하리라고 보여진다.