• Journal of Photoscience
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• v.12 no.3
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• pp.143-147
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• 2005

The simultaneous determination of harman, harmaline and norharman has been described using synchronous fluorescence technique. The method has been based on their natural fluorescence. It is difficult to analyze and determine their contents by conventional fluorescence method because of their similar molecular structures. The synchronous spectrum, maintaining a constant wavelength difference of ${\Delta}{\lambda}=185nm$ between the excitation and emission monochromators, was selected as optimum to perform the determination. The method was also performed in aqueous medium at pH 4.0 and in presence of sodium dodecyl sulfate (SDS), $1{\times}10^{-5}M$. Under the optimum conditions, each analyte has the linear determination range of $1{\times}10^{-7}M-\;1{\times}10^{-4}M$.

• Preventive Nutrition and Food Science
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• v.1 no.1
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• pp.106-110
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• 1996

Bromelain(EC 3.4.22.4), the collective name for the proteolytic enzymes found in tissues of the plant family Bromeliaceae(pineapple), has been used as a tenderizing agent in food processing, and as an antiinflammatory agent in pharmaceuticals. In this paper, we describe the simple purification method of bromelain using Korean pineapple fruit. After removing contaminants at 30% saturation of ammonium sulfate, the supernatant obtained was treated again with ammonium sulfate to 80% saturation. Wit the above salt fractionation, partially purified bromelain could be obtained. To get highly purified bromelain, the previous 30% to 80% ammonium sulfate treated precipitate was dialyzed against 25mM sodium acetate buffer(pH 5.0) followed by passing through a CM- cellulose cation exchange column. Fruit bromelain was eluted as a major peak at 0.5~0.8M NaCI gradient. The present method is simpler with high wield than the traditional purification method-acetone treatment and several consecutive chromatographic processes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.4
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• pp.253-259
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• 1996

Ammonia microdiffusion method and colorimetric measurement are described for the nitrogen determination. The diffusion of ammonia could be successfully induced by using a microdiffusion cell. It is a simple and rapid technique, which is suitable for transforming the nitrogen in digests into $NH_4CI$ for the colorimetric N determination with ammonia color reagent. Above 99% of N recovery were obtained with microdiffusion up to 15 hours. The coloration method of collected $NH_4CI$ for the colorimetric N determination was also estabilshed with a scanning in U.V. spectrophotometer. Under the proposed coloration method (0.5 mL of sample digest, 4.0 mL of $H_2O$ and 0.5 mL of ammonia color reagent), a maximal absorbance was observed at 410 nm. The kinetic measurement of absorbance showed a high stability from 5 to 45 minutes after color development. Absorbance was directly proportional to the amount of $NH_4^+-N$ present. The microdiffusion-ammonia coloration method was successfully applied to the nitrogen determination in the forms of protein-N or total -N in plant tissue. Comparing with Kjeldahl distillation method, the values obtained with described method were slightly higher and more reliable.

• Bulletin of the Korean Chemical Society
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• v.26 no.5
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• pp.781-785
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• 2005

A fast and reliable method for the selective separation and preconcentration of $Cu^{2+}$ ions using homogeneous liquid-liquid extraction was developed. A new $\alpha$-dioxime derivative (2H-1,4-benzothioazine-2,3(4H)dionedioxime, Dioxime I) was synthesized and investigated as a suitable selective complexing ligand for $Cu^{2+}$ ions. Zonyl FSA (FSA) was applied as a phase-separator agent under the slightly acidic pH conditions. Under the optimal experimental conditions ([FSA] = 3.2% w/v, [THF] = 19.5% v/v, [Dioxime I] = 1.9 ${\times}\;10^{-3}$ M, and pH = 4.7), 10 ${\mu}g\;of\;Cu^{2+}$ in 5.2 mL aqueous phase could be extracted quantitatively into 80 $\mu$L of the sedimented phase. The maximum concentration factor was 65-fold. The limit of detection of the proposed method was 0.005 ng $mL^{-1}$. The reproducibility of the proposed method, on the 10 replicate measurements, was 1.3%. The influence of the pH, type and volume of the water-miscible organic solvent, concentration of FSA, concentration of the complexing ligand and the effect of different diverse ions on the extraction and determination of $Cu^{2+}$ ions were investigated. The proposed method was applied to the extraction and determination of $Cu^{2+}$ ion in different synthetic and natural water samples.

• Journal of the Korean Home Economics Association
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• v.47 no.4
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• pp.61-72
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• 2009

The purpose of this research is to suggest a new way to approach measuring the waist line of slacks. The pattern formulated enables a construction method that optimizes motion. The method is based on the measurement on the length change of the body surface line. The research reveals: 1. The analysis of expansion and contraction by area showed that G8 markedly shrunk, whilst G15 maximally stretched during M4 motion. 2. The areas that stretched during M2 motion were, in order of size: G10, G17, G16, and G8. Conversely, the areas that shrunk are, in order, G9, G11, and G18. The areas that stretched during M3 motion were G10, G17, G16, G12, and G15; the areas that shrunk were G9, G11, G18, and G8. 3. In constructing the slacks pattern to allow for appropriate movement, we calculated the length between the knee and back of the waist, point (y), using Pythagoras’theorem and trigonometry. The equation was y = 1.005x. 4. In the two pattern N method and L method, y is equal or less than x, but for our research pattern, y was larger than x

• Analytical Science and Technology
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• v.36 no.4
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• pp.152-160
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• 2023

Adenosine and cordycepin are bioactive compounds with health benefits. Therefore, both substances are often used to assess the quality of Cordyceps products. Optimization and validation of the HPLC/DAD method for determining two nucleosides were studied. The samples were prepared using an ultrasound-assisted extraction (ultrasonic bath). The result was optimal conditions for aqueous extraction, an extraction time of 35 min, and an extraction temperature of 40 ℃. The Chromatographic separation was achieved using a reverse phase column (InfinityLab Poroshell 120 EC-C18, 4.6 × 250 mm, 2.7 ㎛) at 30 ℃ with a mobile phase gradient elution of water and methanol at a flow rate of 0.7 mL/min. The eluents were monitored via a diode array detector at 260 nm. Two nucleosides were separated by less than 12 min after injection. The developed method was found to be excellent linear (r² > 0.9999), accurate (% recovery 95.34-98.51), and precise (% relative standard deviation < 2.0). The limit of detection (LOD) and quantification (LOQ) were 0.45 and 1.38 mg/mL for adenosine and 0.47 and 1.43 mg/mL for cordycepin, respectively. This method was satisfactory for simultaneously quantitating two nucleoside contents, which were used to evaluate Cordyceps products.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.12 no.4
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• pp.1-7
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• 2016

This paper presents a design method for the horizontal straight ground heat exchanger (GHE) based on the Kavanaugh design method. In order to examine suitability of the suggested design method, a horizontal line type GHE was installed in a steel box of which the size was $5m{\times}1m{\times}1m$ filled with dried Joomunjin standard, and a thermal response test (TRT) was conducted for 21 hours. A numerical analysis was performed for a simulation of a peak month operation and for its verification by finite element method (FEM). According to the simulation results, it was concluded that the suggested design method for a horizontal straight GHE is reliable for the estimation of a design length.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.4
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• pp.524-532
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• 2012

In this paper, we used MOM-based BIM(Born Iterative Method) algorithm to implement the inverse scattering for the detection of cancer. We adopted two-dimensional breast structure, integral equations and two-dimensional Green's function is solved with MoM(Method of Moment) to analyzing electromagnetic scattering phenomena. In addition, verifying the calculation of developed inverse scattering algorithm and analyzing medical applicability and limitations of the algorithm.

• Ocean Systems Engineering
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• v.7 no.4
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• pp.399-412
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• 2017

The Gauss-Legendre integral method is applied to numerically evaluate the Green function and its derivatives in finite water depth. In this method, the singular point of the function in the traditional integral equation can be avoided. Moreover, based on the improved Gauss-Laguerre integral method proposed in the previous research, a new methodology is developed through the Gauss-Legendre integral. Using this new methodology, the Green function with the field and source points near the water surface can be obtained, which is less mentioned in the previous research. The accuracy and efficiency of this new method is investigated. The numerical results using a Gauss-Legendre integral method show good agreements with other numerical results of direct calculations and series form in the far field. Furthermore, the cases with the field and source points near the water surface are also considered. Considering the computational efficiency, the method using the Gauss-Legendre integral proposed in this paper could obtain the accurate numerical results of the Green function and its derivatives in finite water depth and can be adopted in the near field.

• Journal of the Korean Chemical Society
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• v.44 no.4
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• pp.322-327
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• 2000

A new absorption spectrometric method for the determination of the Cr$_2O_7^{2-}$ ionin acidic media has been developed. The new method is based on the oxidation-reduction reaction of the HCr$O_4^-$ ion with H$_2$O$_2$forming a deep blue CrO(O$_2$) $_2$ andis coupled with a technique of flow injection analysis(FIA). The new method provides a linear calibration curve which accurately follows the Beer's law over a wide range of the analytical concentrations(2.0 ${\times}$ $10^{-6}$M~8.0 ${\times}$ $10^{-3}$M) of K$_2$Cr$_2$O$_2$. The sensitivity of the new method is approximately two times greater than the current method and the effects of the interfering substances such as V, Co, Ni, Fe, and Mn are almost negligible except Cu.