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Motional kinematics of Frozen-thawed Korean native cattle semen use of computer aided semen analysis(CASA) system (컴퓨터 정액자동분석에 의한 동결융해 한우 정액의 운동특성 연구)

  • Lee, Kang-nam;Lee, Byeong-chun;Kim, Jung-tae;Park, Jong-im;Shin, Tae-young;Hwang, Woo-suk
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.898-908
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    • 1998
  • The aim of this experiments were to assess the time-interval change of motional characteristics in frozen-thawed semen of Korean native cattle (KNC) by using computer aided semen analysis (CASA) technology. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system (SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber (Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The measurement parameters in SIAS were as follows ; frame rate = 30 frames per sec, image capture = 1 sec, minimum motile speed = $10{\mu}m/s$, maximum countable sperm number = 400. Statistical analysis was done by Student t-test with use of the Sigma plot program on a IBM personal computer. The dancemean(DNM) and hyperactivated sperm(HYP) of frozen-thawed KNC semen kinematics were significantly decreased(p < 0.05) after 10 min of incubation at $37^{\circ}C$ water bath. But, wobble(WOB) of same sample semen was significantly increased(p < 0.05) after 10 min of incubation and significantly decrease(p < 0.05) after 60 min of same incubation. And, after 30 mim of incubation, significantly differences were found most of motion kinematics, motifity(MOT), curvilinear velocity(VCL), straight line velocity(VSL), average path velocity(VAP), amplitude of lateral head displacement(ALH), beat cross frequency(BCF), mean angular displacement(MAD), dance(DNC), on same sample semen. The DNM of KNC semen sample was variable kinematics after 30 min of incubation. Also, the linearity(LIN) and straightness(STR) was significantly decreased(p < 0.05) from 60 min of incubation. In conclusion, the AI within 30 min after thawing of frozen semen can be an effective method for obtaining high fertility rate in KNC reproductive program.

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Stochastic Programming Model for River Water Quality Management (추계학적 계획모형을 이용한 하천수질관리)

  • Cho, Jae Heon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.14 no.1
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    • pp.231-243
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    • 1994
  • A stochastic programming model for river water quality management was developed. River water quality, river flow, quality and flowrate of the wastewater treatment plant inflow were treated as random variables in the model. Withdrawal for water supply and submerged weir reaeration were included in the model itself. A probabilistic model was formulated to compute the expectation and variance of water quality using Streeter-Phelps equation. Chance constraints of the optimization problem were converted to deterministic equivalents by chance constrained method. Objective function was total annual treatment cost of all wastewater treatment plants in the region. Construction cost function and O & M cost function were derived in the form of nonlinear equations that are functions of treatment efficiency and capacity of treatment plant. The optimization problem was solved by nonlinear programming. This model was applied to the lower Han River. The results show that the reliability to meet the DO standards of the year 1996 is about 50% when the treatment level of four wastewater treatment plants in Seoul is secondary treatment, and BOD load from the tributary inflows is the same as present time. And when BOD load from Tanchon, Jungrangchon, and Anyangchon is decreased to 50%, the reliability to meet the DO standards of the year 1996 is above 60%. This results indicated that for the sake of the water quality conservation of the lower Han River, water quality of the tributaries must be improved, and at least secondary level of treatment is required in the wastewater treatment plants.

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Development of Internal Reforming Catalysts and Catalysts Forming Method using Homogeneous Precipitation (균일용액침전법을 이용한 내부개질촉매의 개발과 성형방법에 대한 연구)

  • Jung, Youshick;Rhee, Youngwoo;Koo, Keeyoung;Jung, Unho;Youn, Wanglai;seo, Yongseog
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.223.2-223.2
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    • 2010
  • 용융탄산염 연료전지(MCFC)는 $650^{\circ}C$에서 작동하는 고온형 연료전지 시스템이다. 이 시스템은 천연가스 등을 개질하여 생산된 수소를 바로 전기로 생산할 수 있는 시스템으로 열효율이 높으며, 현재 대체 발전시스템으로 각광을 받고 있다. MCFC는 개질방식에 따라 내부개질 방식과 외부개질 방식이 있다. 내부개질 방식은 수소를 생산하는 개질기가 스택내부에 장착된 형식으로 천연가스를 스택내부에서 개질하여 바로 전기를 생산하는 방식이다. 이 내부개질반응에 사용되는 촉매로는 알루미나에 담지된 니켈(Ni) 계열촉매이 주로 쓰이고 있다. 또한 내부개질촉매의 형태는 작은 원주형의 촉매형태로 성형되어 사용된다. 이 성형된 촉매의 크기가 바로 내부개질 스택의 크기를 결정하는 중요한 요소이다. 그래서 촉매의 크기는 되도록이면 작게 성형하는 것이 중요하다. 그러나 촉매의 크기가 너무 작으면 촉매를 성형하는데 큰 어려움이 생기게 된다. 본 연구에서는 니켈 촉매를 공침법이 아닌 균일용액침전법을 이용하여 제조하였으며, 이 촉매를 이용하여 지름이 약 2 mm 이하로 촉매를 압출성형하는 방법을 연구하였다. 먼저 요소(urea)를 이용한 균일용액침전법으로 촉매를 제조하였다. 최적의 촉매 합성조건을 살펴보기 위해서, 반응 온도를 80, 85, 90, 95, $100^{\circ}C$로 변화 시키면서 제조된 촉매의 특성을 살펴보았다. 그리고 촉매의 적절한 니켈 양을 알아보기 위해서 니켈의 양을 30, 40, 50, 60, 70 wt%로 변화 시켰으며, 조촉매로 사용되는 MgO 양을 5, 10, 15, 20 wt%로 변화 시켜서 제조된 촉매의 특성을 살펴보았다. 물성을 비교하기 위해서, X-선 회절분석(XRD) 및 TPR, 물리화학흡착을 하였다. 그 결과 침전반응온도가 $80^{\circ}C$에서 촉매가 가장 좋은 물성을 보였으며, 우수한 개질성능을 보였다. 그리고 촉매 활성물질인 니켈의 함량은 50 wt% 정도가 가장 적절한 함량이었으며, MgO의 함량이 15 wt%일 때 가장 우수한 물성과 개질 성능을 보여주었다. 이 촉매들은 공침법으로 제조된 상용촉매와 비교하였을 때, 보다 우수한 물성과 개질성능 보였다. 그래서 이 촉매를 균일침전법을 이용하여 대량으로 제조한 다음 압출성형 방법을 이용하여 촉매를 원주형으로 제조하였다. 먼저 제조된 촉매는 별도의 분쇄작업(볼밀 혹은 제트밀)을 거치지 않아도 입자사이즈가 약 $4{\mu}m$ 수준이 나오도록 촉매 제조조건을 조절 하였다. 그리고 소량의 Methyl cellulose(MC) 바이더와 물만 사용하여 촉매를 혼합한 다음 스크류 압출기를 이용하여 촉매를 성형하였다. 이 촉매는 지름이 약 2 mm 이하로 제조할 수 있었으며, 기계적 강도는 타정기로 성형한 상용촉매보다 우수하였다. 그리고 촉매 성능 또한 상용촉매와 비교하였을 때, 우수한 성능 보였다. MCFC용 내부개질 촉매로 균일용액침전법을 사용한 촉매가 적합하다고 판단되며, 압출성형에도 적합하다고 판단되었다.

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An Analysis of Suicidal Accidents on Psychiatric In-patients (입원중 정신병 환자의 자살사고 요인 분석)

  • 이평숙
    • Journal of Korean Academy of Nursing
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    • v.5 no.2
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    • pp.11-22
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    • 1975
  • Suicides have been considered to be one of the grave problems of modern societies. According to recent police statistics of Republic of Korea, 28.6 suicides in every 100, 000 were reported. Psychiatric Patients are believed to be predisposed to suicidal tendencies. This study was performed to investigate the characteristics of suicidal attempts and to analyse the environmental factors involved in the suicidal accidents of patients admitted to psychiatric hospitals. Records of 66 suicidal accidents from three psychiatric hospitals during the period of January 1971 through June 1971 were sampled. Data were analysed by percentile score. Results are as follows; 1. The age group of 21~30yrs. was revealed to be the highest in frequency of suicidal attempts (50.0%) Among the unsuccessful suicides; the age group of 31~40yrs. in men and the age group of 21~30 yrs. in women revealed to be the highest in frequency-Among the unsuccessful suicides; the age group of 21-30 yrs. in both sex revealed to be the highest in frequency. 2. Suicidal accidents occurred more frequently to the unmarried(63.6%)- Among the successful suicides; higher frequency was shown to unmarried in men and the frequency is contrasted in women. Among the unsuccessful; the unmarried in both sex were revealed to be highest in frequency. 3. Schizophrenia was revealed the highest of suicidal attempts in frequency (81.8%). 4, Suicides were most frequently attempted in the spring(46, 9%). Among the successful suicides; highest frequencies were shown in men in tile winter and in women in the summer season. Among the unsuccessful suicides :highest frequencies were shown in men in the winter and in women in the spring. 5. Suicidal attempts were most frequently occurred in hospital wards (40.9%), In women, unsuccessful attempts were found to be the highest on authorized leave at their homes. 6. The hanging was revealed to be the most frequently adopted methods for suicidal attempts (31.8%). Among the successful suicides; hanging was the most frequent method adopted in men white in women the drug over-dose, Among the unsuccessful suicides ; stabbing by sharp devices while in women drug-overdosage was adopted as well 7. The most frequently adopted instrument of different suicidal attempts were: house-hold wrapper (26.3%) in cases of hanging, knives (31.8%) in cases of stabbing, and drugs. 8. The suicidal attempts have occurred most frequently at dawn through early morning (2-6A. M.) (34.8%). Among the successful suicides i most frequent time of occurrence on week-days were revealed to be dawn, while on holidays the occurrence were in the evening as well Among the unsuccessful; the most frequent time of occurrence was the day hours while on holidays at dawn. 9. Suicidal attempts within the hospital ward were first noticed by nurses most frequently (42.2%). 10. Manifestations such as restlessness, depression, self-depreciation were revealed to be the most frequent pre-suicidal attempt behavior characteristics. 11. Among the successful suicides ; manifestations of physical damage were found on the neck while among unsuccessful attempts, the damages were found on exterminates.

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Xylanase Activity of Bacillus pumilus H10-1 Isolated from Ceratotherium simum Feces (흰 코뿔소 배설물로부터 분리한 Bacillus pumilus H10-1의 Xylanase 활성)

  • Yoon, Young Mi;An, Gi Hong;Kim, Jung Kon;Ahn, Seung-Hyun;Cha, Young-Lok;Yang, Jungwoo;Yu, Kyeong-Dan;Moon, Youn-Ho;Ahn, Jong-Woong;Koo, Bon-Cheol;Choi, In-Hoo
    • KSBB Journal
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    • v.29 no.5
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    • pp.316-322
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    • 2014
  • Xylanase have been used to convert the polymetric xylan into fermentable sugars from the production of ethanol and xylitol from plant biomass. The aim of this study was to isolate and identify xylanolytic bacterium from herbivore feces and was to used the xylanase for enzymatic hydrolysis of biomass. Xylanolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-do. The xylanolytic strains were selected by congo red staining and DNS method. Total 67 strains isolated from the herbivores feces were tested for xylanase activity. Among the strains, H10-1, which has the highest xylanase activity, was isolated from feces of Ceratotherium simum. The H10-1 strain was identified as Bacillus pumilus based on its morphological/biochemical characteristics and partial 16S rDNA gene sequences. Culture conditions of B. pumilus H10-1 such as initial medium pH, incubation temperature and incubation time were optimized for maximum xylanase production. And also xylanase produced by B. pumilus H10-1 was applied for the saccharification of Miscanthus sacchariflorus cv. 'Geodae 1', which was pretreated with 1.5M NaOH. The optimized culture conditions of B. pumilus H10-1 were pH 9, $30^{\circ}C$ incubation temperature, and 7 day incubation time, respectively. This xylanase activity under the optimized conditions was $20.4{\pm}3.3IU$. The crude xylanase produced by B. pumilus H10-1 was used for the saccharification of xylan derived from pretreated 'Geodae 1'. The saccharification conditions were $50^{\circ}C$, 200 rpm, and 5 days. Saccharification efficiency of pretreated 'Geodae 1' by B. pumilus H10-1 was 8.2%.

GQD layers for Energy-Down-shift layer on silicon solar cells by kinetic spraying method

  • Lee, Gyeong-Dong;Park, Myeong-Jin;Kim, Do-Yeon;Kim, Su-Min;Gang, Byeong-Jun;Kim, Seong-Tak;Kim, Hyeon-Ho;Lee, Hae-Seok;Gang, Yun-Muk;Yun, Seok-Gu;Hong, Byeong-Hui;Kim, Dong-Hwan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.422.1-422.1
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    • 2016
  • Graphene quantum dots (GQDs), a new kind of carbon-based photo luminescent nanomaterial from chemically modified graphene oxide (CMGO) or chemically modified graphene (CMG), has attracted extensive research attention in the last few years due to its outstanding chemical, optical and electrical properties. To further extended its potential applications as optoelectronic devices, solar cells, bio and bio-sensors and so on, intensive research efforts have been devoted to the CMG. However, the CMG, a suspension of aqueous, have problematic since they are prone to agglomeration after drying a solvent. In this study, we synthesized the GQDs from graphite and deposited on silicon substrate by kinetic spray. The photo luminescent properties of deposited GQD films were analyzed and compared with initial GQDs suspension. In addition, its carbon properties were investigated with GQDs solution properties. The properties of deposited GQD films by kinetic spray were similar to that of the GQDs suspension in water. We could provide a pathway for silicon-based silicon based device applications. Finally, the well-adjusted GQD films with photo luminescence effects will show Energy-Down-Shift layer effects on silicon solar cells. The GQD layers deposited at nozzle scan speeds of 40, 30, 20, and 10 mm/s were evaluated after they were used to fabricate crystalline-silicon solar cells; the results indicate that GQDs play an important role in increasing the optical absorptivity of the cells. The short-circuit current density (Jsc) was enhanced by about 2.94 % (0.9 mA/cm2) at 30 mm/s. Compared to a reference device without a GQD energy-down-shift layer, the PCE of p-type silicon solar cells was improved by 2.7% (0.4 percentage points).

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Antioxidative Effect of Extracts from Different Parts of Kohlrabi (콜라비 부위별 추출물의 항산화 효과)

  • Pak, Won-Min;Kim, Koth-Bong-Woo-Ri;Kim, Min-Ji;Kang, Bo-Kyeong;Bark, Si-Woo;Kim, Bo-Ram;Ahn, Na-Kyung;Choi, Yeon-Uk;Yoon, Sung-Ryul;Ahn, Dong-Hyun
    • Journal of Applied Biological Chemistry
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    • v.57 no.4
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    • pp.353-358
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    • 2014
  • Antioxidant activities of ethanol and water extracts of flesh, leaves, and peels of green and purple Kohlrabi were measured by rancimat method, total phenolic compound (TPC), DPPH radical scavenging effect, chelating effect, and reducing power analysis. The highest TPC was observed in ethanol extract from green peel and water extract from purple leaf with 12.00 and 11.70 mg/g of dry sample, respectively. The ethanol extracts from purple Kohlrabi leaf and peel exhibited strong DPPH radical scavenging effects with reducing power while water extracts from purple Kohlrabi leaf and peel also showed strong DPPH radical scavenging with chelating effects. Antioxidant index of ethanol and water extracts from green and purple Kohlrabi measured by rancimat was lower than butylated hydroxytoluene. These results suggest that extracts from purple Kohlrabi leaf and peel exhibited higher antioxidant activities than those of green Kohlrabi, and can be potentially used as proper natural antioxidants.

Biomechanical Analysisz of Varying Backpack Loads on the Lower Limb Moving during Downhill Walking (내림 경사로 보행시 배낭 무게에 따른 하지 움직임의 운동역학적 분석)

  • Chae, Woen-Sik;Lee, Haeng-Seob;Jung, Jae-Hu;Kim, Dong-Soo
    • Korean Journal of Applied Biomechanics
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    • v.25 no.2
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    • pp.191-198
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    • 2015
  • Objective : The purpose of this study was to conduct biomechanical analysis of varying backpack loads on the lower limb movements during downhill walking over $-20^{\circ}$ ramp. Method : Thirteen male university students (age: $23.5{\pm}2.1yrs$, height: $175.7{\pm}4.6cm$, weight: $651.9{\pm}55.5N$) who have no musculoskeletal disorder were recruited as the subjects. Each subject walked over $20^{\circ}$ ramp with four different backpack weights (0%, 10%, 20% and 30% of body weight) in random order at a speed of $1.0{\pm}0.1m/s$. Five digital camcorders and two force plates were used to obtain 3-d data and kinetics of the lower extremity. For each trial being analyzed, five critical instants were identified from the video recordings. Ground reaction force, loading rate, decay rate, and resultant joint moment of the ankle and the knee were determined by the inverse dynamics analysis. For each dependent variable, one-way ANOVA with repeated measures was used to determine whether there were significant differences among four different backpack weight conditions (p<.05). When a significant difference was found, post hoc analyses were performed using the contrast procedure. Results : The results of this study showed that the medio-lateral GRFs at RHC in 20% and 30% body weight were significantly greater than the corresponding value in 0% of body weight. A consistent increase in the vertical GRFs as backpack loads increased was observed. The valgus joint movement of the knee at RTO in 30% body weight was significantly greater than the corresponding values in 0% and 10% body weight. The increased valgus moment of 30% body weight observed in this phase was associated with decelerating and stabilizing effects on the knee joint. The results also showed that the extension and valgus joint moments of the knee were systematically affected by the backpack load during downhill walking. Conclusion : Since downhill walking while carrying heavy external loads in a backpack may lead to excessive knee joint moment, damage can occur to the joint structures such as joint capsule and ligaments. Therefore, excessive repetitions of downhill walking should be avoided if the lower extremity is subjected to abnormally high levels of load over an extended period of time.

Age Dependent Behaviors of Composite Girders Subjected to Concrete Shrinkage and Creep (건조수축과 크리프에 의한 합성형 거더의 재령종속적 거동)

  • Ahn, Sung-Soo;Sung, Won-Jin;Kang, Byeong-Su;Lee, Yong-Hak
    • Journal of the Korea Concrete Institute
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    • v.18 no.1 s.91
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    • pp.109-116
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    • 2006
  • An incremental approach to predict the time dependent flexural behavior of composite girder is presented in the framework of incremental finite element method. Age dependent nature of creep, shrinkage, and maturing of elastic modulus of concrete is prescribed in the incremental tangent description of constitutive relation derived based on the first order Taylor series expansion applying to the total from of stress-strain relation. The loop phenomenon in which age dependent nature of concrete causes stress redistribution and it causes creep in turn is taken into account in the formulation through the incremental representation of constitutive relation. The developed algorithm predicts the time dependent deflections of 4.8m long two span double composite box girder subjected to shrinkage, maturing of elastic modulus, and creep initially induced by self weight. Comparison shows a good agreement between the predicted and measured results.

Effect of Application Methods for Liquid Pig Slurry on Growth and Yield of Rice(Oryza sativa L.) (가축 액상분뇨의 시용방법이 벼의 생육과 수량에 미치는 영향)

  • Ryoo J. W;Hong M. Z.
    • Journal of Animal Environmental Science
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    • v.10 no.2
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    • pp.75-80
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    • 2004
  • Field experiments were conducted in 2002 on sandy loam soil under variable fertilizer management; swine slurry and chemical fertilizer. The effect of different fertilizer management and application method on the growth characteristics and yield of rice were determined. The rice was planted to examine the effect of swine slurry on the crop growth. As slurry manure was fertilized, yield of each group of rice was decreased from 3 to $5\%$. In conclusion, basal application of slurry was maintained productivity of rice, but to improve of productivity of rice, the rice should be fertilized with the basal application of animal slurry and supplementary application of chemical fertilizer. The results indicates that application of animal slurry improves quality of rice rather quantity, which means grain yield. Rice quality of the slurry+chemical and the slurry were significantly better than that of the chemical fertilizer. That indicates application of animal slurry could improve rice quality. Application of animal slurry seemingly has potentials for reducing pollution of animal slurry and improving environment of rural area as well as producing high-quality rice.

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