• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.20-25
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• 2009

Xenotransplantation of pig organs into humans is a potential solution for the shortage of donor organs for transplantation. However, multiple immune barriers preclude its clinical application. In particular, the initial type of rejection in xenotransplantation is an acute cellular rejection by host $CD8^+$ cytotoxic T lymphocyte (CTL) cells that react to donor major histocompatibility complex (MHC) class I. The human cytomegalovirus (HCMV) glycoprotein Unique Short (US) 2 specifically targets MHC class I heavy chains to relocate them from the endoplasmic reticulum (ER) membrane to the cytosol, where they are degraded by the proteasome. In this study we transfected the US2 gene into minipig fetal fibroblasts and established four US2 clonal cell lines. The integration of US2 into transgenic fetal cells was confirmed using PCR and Southern blot assay. The reduction of Swine Leukocyte Antigen (SLA)-I by US2 was also detected using Flow cytometry assay (FACS). The FACS analysis of the US2 clonal cell lines demonstrated a substantial reduction in SLA-I surface expression. The level (44% to 76%) of SLA-I expression in US2 clonal cell lines was decreased relative to the control. In cytotoxicity assay the rate of $CD8^+$ T cell-mediated cytotoxicity was significantly reduced to 23.8${\pm}$15.1% compared to the control (59.8${\pm}$8.4%, p<0.05). In conclusion, US2 can directly protect against $CD8^+$-mediated cell lysis. These results indicate that the expression of US2 in pig cells may provide a new approach to overcome the CTL-mediated immune rejection in xenotransplantation.

• 한국게임학회 논문지
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• 제10권5호
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• pp.51-63
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• 2010

본 연구에서는 온라인게임산업의 선도기업인 "엔씨소프트"의 혁신역량을 혁신경로 창출능력의 관점에서 핵심기술 관리역량 6개 지표, 연계/통합 역량 4개 지표, 기술혁신 전략수립/정책대응 역량 3개 지표를 토대로 분석하였다. 각 부분별 이 기업의 대표적 강점으로는 핵심기술관리의 측면에서 글로벌 선도게임개발에 대한 지속적인 R&D투자, 연계/통합전략의 측면에서 해외 R&D인력과 스튜디오에 대한 적극적 투자와 M&A전략, 그리고 기술전략/정책 부문에서 글로벌 기술리더십의 확보를 들 수 있었다. 반면 보완이 필요한 부분으로 핵심기술관리 측면에서 개방형/가치창출형 지식재산관리전략으로 전환과 중장기적인 원천기술개발에 대한 투자 증대, 연계/통합전략 측면에서 공공부문 R&D기관과의 상생적 보완/협력방안 마련 및 국내와 해외 스튜디오 간 신기술 지식 교류 활성화 전략 수립의 필요 그리고 기술전략/정책 부문에서 새로운 사회적 가치를 제공하는 기능성 게임에 대한 투자 증대를 꼽을 수 있었다. 이와 같은 선도기업의 분석결과는 업계 전반의 발전전략 수립에도 시사점을 지닌다고 할 수 있다.

• 비파괴검사학회지
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• 제35권3호
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• pp.200-205
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• 2015

현대의 컴팩트 반도체 소자들은 정확한 품질검사를 위해 비파괴, 고분해능의 검사 장비가 요구되고 있다. 검사 장비 중 고분해능 적외선 대물렌즈와 적외선 센서로 구성된 초정밀 열영상 현미경은 반도체 내부의 결함에서 발생되는 국소적 열원의 위치와 깊이 정보를 얻는데 유용하게 활용되고 있다. 본 연구에서는 위 상잠금기법이 적용된 적외선열영상 현미경을 이용하여 다층구조로 된 반도체 소자 내부 열원의 위치와 깊이 정보에 대해 분석하였다. 시편은 내부에 3개의 열원을 포함한 TSV(through silicon via technology) 기반 4단 적층구조로서 측정 표면으로부터 열원의 깊이는 $240{\mu}m$이다. 본 실험에서는 위상잠금기법을 통해 시편 내부열원의 위치와 깊이를 정확히 찾을 수 있는 초점면 위치, 노출시간 그리고 위상잠금주파수 등 최적의 조건을 찾고 그 조건에서 적외선 대물렌즈와 시편의 거리 변화에 따른 위상 변이와 깊이 정보에 대한 영향을 알아보았다. 이와 같은 반도체 내부결함에 의한 열원의 위치와 깊이 분석에 대한 연구는 품질검사용 열영상 분석장비 개발에 큰 도움을 줄 것으로 예상한다.

• 지능정보연구
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• 제15권4호
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• pp.141-158
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• 2009

다양한 정보자원들로부터 사용자가 요구하는 맞춤화된 정보를 추출해 내는 것은 더욱 어려워지고 있다. RSS를 비롯하여 개선된 다양한 정보 수집 방법들이 개발되었지만, 여전히 정보가공자인 사람의 도움 없이 필요한 정보들을 수집하여 정리 및 가공하는 작업이 쉽지는 않다. 따라서 본 연구에서는 정보사용자들이 사용 목적에 맞게 정보를 가공하는 부담을 줄여주기 위해 사례기반추론과 온톨로지에 기반한 맞춤형 통합정보생성 프레임워크를 제안한다. 본 프레임워크는 세 단계로 구성된다. 첫째, 수집된 웹 정보를 정보가공의 용이성을 위해 사례로 변환한다. 둘째, 동적 유사도 검색을 통해 수집된 사례들로부터 정보 사용자의 동적 요구사항에 적합한 사례를 검색한다. 셋째, 전 단계에서 추출된 사례를 정보사용자의 요구사항에 보다 적합한 지식으로 가공하기 위해 집중 유사도를 적용한다. 본 프레임워크는 여행자들의 정보수집을 위한 여행정보시스템에 적용되어 그 효과를 입증하였다.

• 한국과학교육학회지
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• 제19권2호
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• pp.204-216
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• 1999

이 연구에서는 자연계에 폭넓게 적용되는 기본 개념들을 몇 가지로 단순화시키고, 이것을 통합주제라고 정의하였다. 이것은 자연계를 관통하는 포괄적 개념 혹은 통합적 시각이라 할 수 있다. 관련 문헌을 분석하여 계(系), 상호작용, 조화와 균형, 구조와 기능, 그리고 순환의 다섯 가지 개념을 통합주제로 설정하고, 각 통합주제의 성격을 규명하였다. 그리고 설정된 통합주제의 성격에 부합하는 내용을 구성하여 각 영역별로 학습 주제와 학습 목표를 진술하였다. 제시된 통합과학 내용 구성은 그 형태에 있어서 간학문적 통합, 혹은 다학문적 통합이라 할 수 있으며, 현행 중학과학보다 통합의 심도가 깊다. 그리고 통합주제의 성격에 우선적으로 초점을 맞춰 구성한 것으로 기존의 4영역 안배 원칙에서 벗어났다. 이와 같은 통합과학 교육과정의 내용 구성 방안은 현재 우리 나라의 과학 교육과정에 새로운 관점을 제시하는 의미가 있다.

• 대한한의학회지
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• 제22권2호
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• pp.31-40
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• 2001

This experimental studies was to investigate location of labeled neurons in CNS following injection of pseudorabies virus(PRV), Bartha strain, into the uterus and Sanyinjiao(Sp6) of rats. After survival times of 4-5 days following the injection of PRV, the rats were perfused, and their brain and spinal cord were frozen sectioned($30\mu\textrm{m}$). These sections were stained by PRV immunohistochemical staining methods, and observed with light microscope. The results were as follows: 1. In the spinal cord, overlap areas of PRV labeled neurons projecting to uterus and Sp6 were observed in lamina VII, IX and X areas of cervical segments. In thoracic segments, overlap areas were observed in lamina IV, VII, X and intermediolateral n.. In lumbar segments, overlap area of PRV labeled neurons were observed in lamina I, V-VII, IX, X and intermediolateral n.. In sacral segments, overlap areas of PRY labeled neurons were observed in lamina N, V, VII, X and sacral parasympathetic n.. 2. In the brain, overlap areas of PR V labeled neurons projecting to the uterus and Sp6 were observed in lateral paragigantocellular n., rostroventrolateral reticular n., raphe obscurus n., raphe pallidus n., raphe magnus n., locus coeruleus n., Barrington's n., A5 cell group, central gray n., paraventricular hypothalamic n. and arcuate n. This results suggest that overlap areas of PRV labeled neurons of the spinal cord projecting to the uterus and Sp6 might be the first-order neurons related to the viscera-somatic sensory and sympathetic preganglionic neurons. PRV labeled neurons of the brain may be the second and third-order neurons response to the movement of smooth muscle of uterus. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory and motor system monitoring the internal environment. These overlap areas of spinal cord and brainmay be related to autonomic centers related to regulation of uterus.

• The Journal of Korean Physical Therapy
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• 제13권3호
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• pp.509-527
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• 2001

The Sun's ray is composed of Infrared(49%), Visible light(40%) and Ultra violet(11%), however the ray getting to the earth is FIR(Far infrared; 60%), IR(Infrared; 20%), and UV(Ultra Violet; 20%). Human beings has utilized FIR already from time immemorial. Hershel found out Infrared for the first time. in the Industrial Revolution the Infrared and FIR had been begun to use making products. In these days, with contemporary science FIR would be begun to clear up the implication in the human body and organic compound. IR classified by wavelength three parts NlR, MIR, FIR. There is FIR which is radiated from healthy human body the wave length is 8-l4m. The human body is composed of proteins which get easily changed by a thermal factor (about 42 $^{\circ}$C over). FIR with low temperature can deeply penetrate on the human body composed things without troublesomes, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). When FlR penetrated on the human body. it would inhibit the abnormal genes and cells expression, and then information of DNA and RNA would be reexpressed for arranging DNA and RNA abnormal state. As FlR's receptors in the body, it could be presumed that N-glycosyl linkage of purine and deoxyribose, RNA splicing process, and Heat shock protein. To take the FIR which was a optimized wavewlength and strength, at first, we induced the characteristic algorithm and the computerized programing. Then we formed that the formular of optimized FIR with physical, mathematical logic and theory. especially, Plank, Kirchhoff, Wien, Stefan-Boltzmann's logic and law. In the long run, the formular was induced with integration mathematical, since we had to know the molecular wavelength. Based on the induced formular as above, we programmed the optimized FlR radiating computerized program. In this research, we designed the eletronic circuit f3r interfacing with human body to diagnosis and treatment with FIR sensor which radiated FIR wavelength optimized.

• 대한의용생체공학회:의공학회지
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• 제31권4호
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• pp.292-301
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• 2010

To restore visual sensation of blind patients, we have proposed a fully implantable retinal prosthesis comprising an three dimensionally (3D) stacked retinal chip for transforming optical signal to electrical signal, a flexible cable with stimulus electrode array for stimulating retina cells, and coupling coils for power transmission. The 3D stacked retinal chip is consisted of several LSI chips such as photodetector, signal processing circuit, and stimulus current generator. They are vertically stacked and electrically connected using 3D integration technology. Our retinal prosthesis has a small size and lightweight with high resolution, therefore it could increase the patients` quality of life (QOL). For realizing the fully implantable retinal prosthesis, we developed a retinal prosthesis module comprising a retinal prosthesis chip and a flexible cable with stimulus electrode array for generating optimal stimulus current. In this study, we used a 2D retinal chip as a prototype retinal prosthesis chip. We fabricated the polymide-based flexible cable of $20{\mu}m$ thickness where 16 channels Pt stimulus electrode array was formed in the cable. Pt electrode has an impedance of $9.9k{\Omega}$ at 400Hz frequency. The retinal prosthesis chip was mounted on the flexible cable by an epoxy and electrically connected by Au wire. The retinal prosthesis chip was cappted by a silicone to pretect from corrosive environments in an eyeball. Then, the fabricated retinal prosthesis module was implanted into an eyeball of a rabbit. We successfully recorded electrically evoked potential (EEP) elicited from the rabbit brain by the current stimulation supplied from the implanted retinal prosthesis module. EEP amplitude was increased linearly with illumination intensity and irradiation time of incident light. The retinal prosthesis chip was well functioned after implanting into the eyeball of the rabbit.

• 대한원격탐사학회지
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• 제27권3호
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• pp.339-351
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• 2011

This study investigates the potentials of satellite, ground measurement data, and geo-spatial information within an urban area for the mapping of the Urban Environmental Quality (UEQ) parameters. The UEQ indicates a complex and various parameters resulting from both human and natural factors, which are greenness, climate, air pollution, the urban infrastructure, and etc. Multi-spectral remote sensing data from the Landsat ETM and TM sensors for the mapping of air pollution by the Haze Optimized Transform (HOT) technique, Urban Heat Island (UHO using the emissivity-fusion method in Seoul from 2000 to 2006 in fine resolution (30m) were analyzed for the estimation of UEQ index. Although the UHI values are similar ($8.4^{\circ}C{\sim}9.1^{\circ}C$) during these years, the spatial coverage of "hot" surface temperature (> $24^{\circ}C$) significantly increased from 2000 to 2006 due to the rapid urban development. Furthermore, high correlations between vegetation index and land surface temperature were achieved with a correlation coefficients of 0.85 (2000), 0.81 (2001), 0.84 (2002), and 0.89 (2006), respectively. It was found that the proposed method was successfully analyzed spatial structure of the UEQ and the scenarios of the best and worst areas within the city were also identified. Based on the quantifiable fine resolution satellite image parameters, UEQ can promote the understanding of the complex and dynamic factors controlling urban environment.

• Molecular & Cellular Toxicology
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• 제1권4호
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• pp.237-247
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• 2005

Several features of the implant surface, such as roughness, topography, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of various thin layer hydroxyapatite (HA) coatings on anodized Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on A (100 nm HA coating on anodized surface), B (500-700 nm HA coating on anodized surface), C ($1{\mu}m$ HA coating on anodized surface), and control (non HA coating on anodized surface) Ti. The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the four dental substrate types. MG63 cells cultured on A, C and control exhibited cell-matrix interactions. It was B surface showing cell-cell interaction. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.