• Title/Summary/Keyword: 4M Integration

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A Routine System for Generation of Fertile Transgenic Rice Plants Using Biolistic Method

  • Lee Soo-In;Kim Cha-Young;Lim Chae-Oh;Choi Young-Ju;Kim Ho-Il;Lee Sang-Yeol;Lee Sung-Ho
    • Journal of Plant Biotechnology
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    • v.5 no.3
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    • pp.163-168
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    • 2003
  • A routine system based on particle bombardment of embryogenic callus for recovery of fertile transgenic rice (Oryza sativa L.) plants was developed. Embryogenic callus was established within 2-3 months from calli derived from mature seeds of Korean rice cultivar, Nagdongbyeo. The callus was bombarded with the plasmid pRQ6 containing the $\beta$-glucuronidase gene (gusA) and hygromycin phosphotransferase gene (hph, conferring resistance to hygromycin B), both driven by CaMV 35S promoter. Placement of cells on an osmoticum-containing medium (0.2 M sorbitol and 0.2 M mannitol) 4 hrs prior to and 16 hrs after bombardment resulted in a statistically significant increase with 3.2-fold in transient expression frequency gusA. In five independent experiments, the average frequency of transformation showing GUS activities was $8.86\%$. A large number of morphologically normal, fertile transgenic rice plants were obtained. Integration of foreign gene into the genome of $R_0$ transgenic plants was confirmed by Southern blot analysis. GUS and HPT were detected in $R_1$ progeny and Mendelian segregation of these genes was observed in $R_1$ progeny.

Optical AND/OR gates based on monolithically integrated vertical cavity laser with depleted optical thyristor (집적화된 광 싸이리스터와 수직구조 레이저를 이용한 광 로직 AND/OR 게이트에 관한 연구)

  • Choi, Woon-Kyung;Kim, Doo-Gun;Kim, Do-Gyun;Choi, Young-Wan
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.43 no.12 s.354
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    • pp.40-46
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    • 2006
  • Latching optical switches and optical logic gates AND and OR are demonstrated, for the first time, by the monolithic integration of a vertical cavity lasers with depleted optical thyristor structure, which have not only a low threshold current with 0.65mA, but also a high on/off contrast ratio more than 50dB. By simple operating technique with changing a reference switching voltage, this single device operates as two logic functions, optical logic AND and OR. The thyristor laser fabricated using the oxidation process achieved a high optical output power efficiency and a high sensitivity to the optical input light.

Hydroacoustic Observations on the Diel Distribution and Activity Patterns of Fishes in the East China Sea II - Vertical Speed of Migration and Variation in Scattering Strength - (동중국해에 있어서 어족생물의 일주기적 여영행동특성에 관한 연구 II - 연직이동속도 및 산란강도의 변동 -)

  • 이대재
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.30 no.4
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    • pp.251-262
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    • 1994
  • The speed of vertical migration and the volume backscattering strength of the scattering layers during the evening and morning transitions between day and night were measured in November 1990-1992 in thermally stratified waters of the East China Sea. Acoustical measurements were carried out using a scientific echo-sounder operating at t재 frequencies of 25 and 100kHz, and using an echo-integration system connected with a micro-computer. Biological sampling was accomplished by bottom trawling to identify fish species recorded on the echo sounder, and the species and length compositions were determined. The values of scattering strength were allocated to group of fishes according to the fish traces on the echo recording paper and the species composition of trawl catches. The vertical velocities of migration derived from the changes in the depths and the values of peak scattering strength of the dense layer vertically migrating toward the bottom or toward the surface. The trawl data suggest that snailfish and fishing frog were the most abundant fishes in all research stations. As sunrise approached, the fish formed a strong concentration just above the thermocline. The the highest values of scattering strength in the entire water column appeared in the depth strata above the thermocline just before the begining of downward migration. As soon as the fish began to migrate downwards across the thermocline, the values of the scattering strength in the depth strata above the thermocline rapidly decreased, while the values for the scattering layer moving slowly toward the bottom gradually increased. During the 1992 surveys, the speed of the vertical migration was estimated to be 0.38m/min in the upward migration and 0.32m/min in the downward migration, respectively. That is the rate of vertical migration was slightly higher at dusk than at dawn. Similar migration patterns were observed on different stations and under different weather conditions during the surveys in 1990.

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An Information System Implementation Case Research on Korea Train eXpress (고속철도에 있어서의 정보시스템 구현 사례에 대한 연구)

  • Lee, Sung-Ho;Ahn, Joong-Ho
    • Information Systems Review
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    • v.7 no.2
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    • pp.1-21
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    • 2005
  • Integrated Railroad Information System(IRIS) Construction Project is one of the super projects in Korea required to invest over 100 billion won and 10,000 engaged manpower until Dec 30, 2004. Composition of this paper is as follows; firstly, examined individual contents and aspects of IRIS, and studied formality and contents that have been based to project methodology for successful propulsion of project in overall situation of SI project progress until present. Secondly, presented for construction background of IRIS as well as Project Management and Project Development Methodologies. Thirdly, described each characteristic and development substance for individual systems after present whole contents and system image of the system. Finally, presented successful propulsion result of this project and development directions. We presented development example laying stress on IRIS's Project contents and propulsion circumstances. Evaluation about operation results should be analyzed and studied in detail operating system from now on.

Breeding of Aspergillus oryzae for the Alkaline Pretense Overproducing Strain. (재조합 Alkaline Protease를 대량 생산하는 Aspergillus oryzae 균주개발)

  • 이병로;유기원;최원균;최동성;임한진;성창근
    • Microbiology and Biotechnology Letters
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    • v.26 no.5
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    • pp.450-455
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    • 1998
  • Aspergillus oryzae M-2-3 strain (argB$\^$-/) was transformed with pTAalp plasmid which was constructed for expression of the alkaline pretense gene, alpA, and 16 transformants were selected on arginine minus medium. When these transformants were tested for productivity of alkaline proteases using agar plate containing skim milk, the halo was observed around each colony of transformants, but not observed around the host strain in this condition. Southern analysis showed that the pTAalp plasmid having alpA gene was integrated into the chromosome of the host strain. The highest level of alkaline protease production was obtained in the culture filtrate of the transformant No. 14, which was estimated to 80-90% of total secreted proteins, and the enzyme activity was 64-450 times higher than those of host strain and industrial strain. Total nitrogen content and the digestion rate in soybean Koji extracts were also increased to 1.5 times in Aspergillus oryzae transformant No. 14.

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EFFECT OF VARIOUS INODIZING CHARACTERISTICS ON BONE INTEGRATION OF TITANIUM IMPLANT SURFACE DESIGN (다양한 양극산화막 처리방법이 임프란트 골유착에 미치는 영향)

  • Cha, Soo-Ryun;Lee, Jun;Min, Seung-Ki
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.30 no.5
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    • pp.417-427
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    • 2008
  • The aim of this study is to investigate the effect of anodizing surface to osseointegration of implant by using of resonance frequency analysis (RFA), quantitative and qualitative assessment of an anodically modified implant type with regard to osseous healing qualities. A total of 96 screw-shaped implants were prepared for this study. 72 implants were prepared by electrochemical oxidation with different ways. 24 (group 1 SP) were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid. 24 (group 2GC) were prepared at galvanostatic mode in calcium glycerophosphate and calcium acetate and 24 (group 3 CMP (Calcium Metaphosphate) Coating were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid followed by CMP coating. Rest of 24 (control group were as a control group of RBM surface. Bone tissue responses were evaluated by resonance frequency analysis (RFA) that were undertaken at 2, 4 and 6 weeks after implant placement in the mandible of mini-pig. Group 1 SP (anodized with sulfuric acid and phosphoric acid implants) demonstrated slightly stronger bone responses than control Group RBM. Group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) demonstrated no difference which were compared with control group. Group 3 GMP (anodized and CMP coated implants) demonstrated slightly stronger and faster bone responses than any other implants. But, all observation result of RF A showed no significant differences between experimental groups with various surface type. Histomorphometric evaluation demonstrated significantly higher bone-to-implant contact for group 2 GC. Significantly more bone formation was found inside threaded area for group 2 GC. It was concluded that group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) showed more effects on the bone tissue responses than RBM surface in initial period of implantation. In addition, CMP showed a tendency to promote bone tissue responses.

Internationalismen der Sprache-Eine Untersuchung der Lexemformen in $europ\"{a}ischen$ Sprachen (언어의 국제주의에 관한 고찰 - 유럽어에서의 어휘소 형태 분석)

  • Lee Jinhee
    • Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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    • v.1
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    • pp.229-245
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    • 1999
  • Mehrere Sprachen insgesamt oder in Ausschnitten miteinander zu vergleichen, hat die Sprachwissenschaft mit je unterschiedlichen Erkenntnisinteressen wenigstens seit dem 17. Jahrhundert unternommen. Bis in diese Zeit reichen die Versuche einer inhaltlichen, d.h. auf die Inhalte sprachlicher Formen bezogene Sprachtypologie zuruck. Im 19. jahrhundert entstand und entwickelte sich die historisch-vergleichende (komparatistische) Sprachwissenschaft mit dem vorrangigen Bestreben, Ursprung, Entwicklungsgeschichte und Verwandtschaftsbeziehungen einzelner Sprachen durch vergleichende Untersuchungen zu rekonstruieren. Die sprachtypologischen $Bem\"{u}hungen\;fr\"{u}herer$ Zeiten haben ihre Forsetzung in der neueren, etwa in den 60er Jahren $verst\"{a}rkt$ einsetzenden Unversalienforschung. In den meiseten $europ\"{a}ischen$ Sprachen gibt es nicht geringe und $auff\"{a}llige$ Lehnwortbestande. Diese $Wortbest\"{a}nde$ beruhen nicht auf $Zuf\"{a}lligkeiten$, sondern sie sind Ergebnisse internationaler Kontakte mit politischen, kulturellen, okonomischen und anderen Voraussetzungen. Diese sprachlichen Gemeinsamkeiten in verschiedenen Sprachen werden mit dem Begriff des Internationalismus $erfa{\ss}t$. Obwohl die $europ\"{a}ischen$ Sprachen sich immer mehr von den gemeinsamen Ursprungssprachen entfernt haben, verraten die $Wortbest\"{a}nde$ der Internationalismen Tendenzen der $Ann\"{a}herungen$ und der Gemeinsarnkeiten, vor allem auf der Ebene des Wortschatzes. Im Ausblick auf $M\"{o}glichkeiten$ der Verwertung von nationalen $Wortsch\ddpt{a}tzen$ kann man so fomulieren: Internatonalismen 1) $k\"{o}nnen$ die Alltags­kommunikation zwischen Menschen verschiedener Herkunftssprachen erleichtern; sie offenbaren, $da\ss$ viele Fremdsprachen gar nicht so fremdartig sind; 2) haben im Hinblick auf den Fremdsprachenerwerb und den Fremdsprachenunterricht multiplizierten Gebrauchwert: Viele Interlexeme $geh\"{o}ren$ zum festen Bestand $europ\"{a}ischer$ Sprachen; 3) geben $Aufschlu\ss\;\"{u}ber$ geschichtliche und kulturelle $Kontaktvorg\"{a}nge$ und Kontaktergebnisse in Europa; 4) $k\"{o}nnen\;als\;Wortsch\"{a}tze\;einer\;m\"{o}glichen\;europ\"{a}ischen$ Integration betrachtet werden'.

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Self-sustained n-Type Memory Transistor Devices Based on Natural Cellulose Paper Fibers

  • Martins, Rodrigo;Pereira, Luis;Barquinha, Pedro;Correia, Nuno;Goncalves, Goncalo;Ferreira, Isabel;Dias, Carlos;Correia, N.;Dionisio, M.;Silva, M.;Fortunato, Elvira
    • Journal of Information Display
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    • v.10 no.4
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    • pp.149-157
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    • 2009
  • Reported herein is the architecture for a nonvolatile n-type memory paper field-effect transistor. The device was built via the hybrid integration of natural cellulose fibers (pine and eucalyptus fibers embedded in resin with ionic additives), which act simultaneously as substrate and gate dielectric, using passive and active semiconductors, respectively, as well as amorphous indium zinc and gallium indium zinc oxides for the gate electrode and channel layer, respectively. This was complemented by the use of continuous patterned metal layers as source/drain electrodes.

The study of a novel SWRO-PRO hybrid desalination technology (SWRO-PRO 복합해수담수화 신공정기술의 연구)

  • Kim, Jisook;Yeo, Inho;Lee, Wonil;Park, Taeshin;Park, Yonggyun
    • Journal of Korean Society of Water and Wastewater
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    • v.32 no.4
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    • pp.317-324
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    • 2018
  • SWRO-PRO hybrid desalination technology is recently getting more attention especially in large desalination markets such as USA, Middle East, Japan, Singapore, etc. because of its promising potential to recover a considerable amount of osmotic energy from brine (a high-concentration solution of salt, 60,000 - 80,000 mg/L) and also to minimize the impact of the discharged brine into a marine ecosystem. By the research and development of the core technologies of the SWRO-PRO desalination system in a national desalination research project (Global MVP) supported by Ministry of Land, Infrastructure, and Transport (MOLIT) and Korea Agency for Infrastructure Technology Advancement (KAIA), it is anticipated that around 25% of total energy consumption rate (generally 3 to $4kWh/m^3$) of the SWRO desalination can be reduced by recovering the brine's osmotic energy utilizing wastewater treatment effluent as a PRO feed solution and an isobaric pressure exchanger (PX, ERI) as a PRO energy converter. However, there are still several challenges needed to be overcome in order to ultimately commercialize the novel SWRO-PRO process. They include system optimization and integration, development of efficient PRO membrane and module, development of PRO membrane fouling control technology, development of design and operation technology for the system scaling-up, development of diverse business models, and so on. In this paper, the current status and progress of the pilot study of the newly developed SWRO-PRO hybrid desalination technology is discussed.

Biolistic transformation of Moroccan durum wheat varieties by using mature embryo-derived calli

  • Senhaji, Chaimae;Gaboun, Fatima;Abdelwahd, Rabha;Diria, Ghizlane;Udupa, Sripada;Douira, Allal;Iraqi, Driss
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.246-254
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    • 2021
  • Environmental stresses are estimated to have reduced global crop yields of wheat by 5.5%. However, traditional approaches for the transfer of resistance to these stresses in wheat plants have yielded limited results. In this regard, genetic transformation has undoubtedly opened up new avenues to overcome crop losses due to various abiotic stresses. Particle bombardment has been successfully employed for obtaining transgenic wheat. However, most of these procedures employ immature embryos, which are not available throughout the year. Therefore, the present investigation utilized mature seeds as the starting material and used the calli raised from three Moroccan durum wheat varieties as the target tissue for genetic transformation by the biolistic approach. The pANIC-5E plasmid containing the SINA gene for drought and salinity tolerance was used for genetic transformation. To enhance the regeneration capacity and transformation efficiency of the tested genotypes, the study compared the effect of copper supplementation in the induction medium (up to 5 μM) with the standard MS medium. The results show that the genotypes displayed different sensitivities to CuSO4, indicating that the transformation efficiency was highly genotype-dependent. The integration of transgenes in the T0 transformants was demonstrated by polymerase chain reaction (PCR) analysis of the obtained resistant plantlets with primers specific to the SINA gene. Among the three genotypes studied, 'Isly' showed the highest efficiency of 9.75%, followed by 'Amria' with 1.25% and 'Chaoui' with 1%.