• 한국가축번식학회지
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• 제25권4호
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• pp.305-315
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• 2001

본 연구의 목적은 외래 EGFP 유전자를 분화이전의 웅성생식세포에 도입한 후 이를 난모세포내에 미세주입하여 형질전환동물을 생산하는 기술을 개발하는 데에 있다. 이를 위하여 반수체 정자세포에서 특이적으로 발현하는 생쥐의 mTP1과 햄스터의 hPrm2 유전자 발현 시기를 RT-PCR로 조사한 결과 그시기는 생쥐와 햄스터에서 각각 18일령과 20일령으로 확인되었다. 이에 따라 외래 유전자의 침입이 용이한 감수분열 직전단계인 17일령의 생쥐와 19일령의 햄스터 정자세포를 EGFP 유전자가 포함된 배양액에 부유시킨 다음, 전기자극을 부여한 결과 0.18 ㎸/cm의 전기자극을 가한 후 72시간 배양한 정자세포의 28.5%와 32.1%에서 EGFP 유전자가 발현되는 것으로 확인되었다. EGFP유전자가 도입된 반수체 정자의 수정 및 발달 능력을 검증하기 위하여, 이들 정자세포를 햄스터 난자 내에 미세주입 하였으나, 형광현미경하에서는 EGFP유전자의 발현은 관찰할 수 없었다. 이에 이들 난자를 공시하여 PCR분석을 실시한 결과, 약 44%의 수정란에서 EGFP 유전자의 존재가 확인되었다. 이러한 결과로 보아 반수체 정자세포는 외래 유전자를 난자 내에 도입하기 위한 운반체로 이용될 수 있을 것으로 생각된다.

• 대한후두음성언어의학회지
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• 제14권2호
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• pp.117-122
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• 2003

Background and Objectives : $VoCoM^{\circledR}$ is a commercialized set composed of prefabricated hydroxylapatite implants and shims of various sizes which are specially designed for the type I thyroplasty. Even though a previously published preliminary report showed that $VoCoM^{\circledR}$ is a convenient and safe product for the type I thyroplasty, further investigations or experiences are yet to be reported. Authors aimed to evaluate the efficacy of $VoCoM^{\circledR}$type I thyroplasty, and its advantage and/or disadvantage. Materials and Method : Twenty three consecutive patients with unilateral vocal cord palsy enrolled for the study, who received type I thyroplasty with $VoCoM^{\circledR}$ between July 2001 and June 2003. Acoustic, aerodynamic and stoboscopic analyses were performed prior to surgery and 1 to 3 months after surgery. Speech language pathologists evaluated their voice quality by GRBAS scale, and patients themselves reported subjective changes of their voice by visual analog scale. Results : The average time for the operation was 80 min, which is about 30 min less than other methods. Preoperative jitter was 3.25$\pm$1.65% and improved to 1.94$\pm$1.79% postoperatively (p<0.05). Preoperative shimmer was 9.72$\pm$6.56% and improved to 5.61 $\pm$3.76% (p<0.05), Maximal phonation time increased from 4.41$\pm$2.99 to 7.98$\pm$4.35 sec (p<0.05) The postoperative stroboscopy revealed an effective medialization in 91.3% of the patients. The subjective phonetic improvements were reported in 21 out of 23 patients. GRBAS scale improved from 2.71$\pm$0.46 to 1.47$\pm$1.12(p<0.05). Additional medialization with $Gore-Tex^{\circledR}$ was easily performed in two revision cases. Previously inserted $VoCoM^{\circledR}$ implant was hard to remove because of the tight integration of soft tissues around the implant. Side effects such as extrusion or foreign body reaction are not observed. $VoCoM^{\circledR}$ was relatively expensive and costed more than 10 times of $Gore-Tex^{\circledR}$. Conclusion : Prefabricated hydroxylapatitie implant($VoCoM^{\circledR}$) provides a convenient, safe and efficient way of vocal fold medialization. However it is relatively expensive and hard to remove.

• 한국ITS학회 논문지
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• 제18권6호
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• pp.164-175
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• 2019

사업용 차량은 교통안전법상 난폭운전 및 사고 방지를 위해 운행기록계, 블랙박스, ADAS를 의무적으로 장착하여야 한다. 한편 도로 위 위험요소들로 인한 피해는 2013년 대비 2016년에 1.5배로 증가하고 있으며 이에 국토교통부는 도로위험정보를 수집할 수 있는 센서를 개발하고 사업용 차량에 장착하여 프로브 차량으로 활용할 수 있는 기술을 개발 중에 있다. 본 연구는 이러한 기술개발을 대비하여 주행속도에 따른 GPS 오차 편차 발생 여부를 확인하고 이벤트 지점을 통합·분리할 적정 통합 범위(m)를 도출하는 분석을 수행하였다. 그 결과 주행속도가 100km/h일 때 평균오차는 9.72m로 주행속도가 40km/h일 때 평균오차 4.69m에 비해 오차가 약 2.1배 커지는 것으로 나타났다. 이벤트 지점 적정 통합·분리 범위(m)는 주행속도와 관계없이 동일지점 인식률 90% 이상인 20m로 분석되었다. 본 연구결과는 사업용 차량이 수집할 위치기반 정보 정확도 향상 및 정책개발의 기초자료로 활용될 수 있을 것이다.

• 한국실내디자인학회논문집
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• 제26권3호
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• pp.63-70
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• 2017

The purpose of this study is to analyze the inner space of detached houses from 1980 to 2016. It was used 'space syntax program' for analysis and analyzed with intervals of 10 years as 1980~1990, 1990~2000 and so on. The research results will be summarized as follows: 1) Overall, all inner space that was examined shows a trend that integration space tends to increase while segregating space tends to decrease. 2) In the case of the master bedrooms(M B) in 1980s, these were composed of 2 rooms as a front room(F R) and a master bedroom type, but as time went by, the F R which was attached room to the M B disappeared. While inside space of M B attached powder-room and wardrobe instead of the F R of the M B. And F R was an integrated space at first in 1980s, but it began to change to a segregating space to protect personal privacy. 3) The dining(D) and kitchen(K) spaces were strictly separated in the 1980s but began to change to D+K and L+D+K types after 1990. 4) In the 2000s, the most typical living space in every house is continuing to change from an separate space in the other space to an integrated space where it is clearly seen from the kitchen and the dining room in the types as L+D and L+D+K.

• 한국전문물리치료학회지
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• 제15권4호
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• pp.27-33
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• 2008

The purpose of this study was to investigate the changes of the center of pressure (COP) trajectory in healthy elderly subjects while crossing an obstacle before and after participation in Tai Chi training. Forty healthy elderly subjects participated either in a 12-week intervention of Tai Chi training or in a health education program. The participants were divided into two groups (the experimental group and the control group). Subsequently, the participants were pre- and post-tested on crossing over an obstacle from a quiet stance. Participants in the experimental group received Tai Chi training that emphasized the smooth integration of trunk rotation, a shift in weight bearing from bilateral to unilateral support and coordination and a gradual narrowing of the lower-extremity stance three times weekly. The participants in the control group attended a health education program one hour weekly and heard lectures about general information to promote health. Performance was assessed by recording the changes in the displacement of the COP in the anteroposterior (A-P) and mediolateral (M-L) directions using a force platform. Participants in the Tai Chi group significantly increased the A-P and M-L displacement of the COP after Tai Chi training (p<.05). No significant differences in the A-P and the M-L displacement of the COP between pre-testing and post-testing in the control group were found. This study has shown that participation in Tai Chi exercise increased the magnitude of the A-P and M-L displacement of the COP, thereby improving the ability of healthy elderly participation to generate momentum to initiate gait. These findings support the use of Tai Chi training as an effective fall-prevention program for the elderly.

• Journal of Advanced Research in Ocean Engineering
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• 제3권4호
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• pp.193-203
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• 2017

This study investigated the steady-flow effects present in the numerical computation of the resistance added to a ship in waves. For a ship advancing in the forward direction, a time-domain 3D Rankine panel method is applied to solve the ship motion problem, and the added resistance due to waves is calculated using a near-field method, with the direct integration of the second-order pressure on the hull surface. In the linear potential theory, the steady flow is approximated by the basis potential of a uniform flow or double-body flow in order to linearize the boundary conditions. By applying these two different linearization schemes, the coupling effects between steady and unsteady solutions were examined. Furthermore, in order to analyze the steady-flow effects on the hull geometry, the computation results for two realistic hull forms, a KVLCC2 tanker and DTC containership, were compared. In particular, the mj term, which represents the coupling effects under the body boundary condition, was evaluated considering the geometry of a non-wall-sided ship. Lastly, the characteristics of the linearization schemes were examined in relation to the disturbed waves around a ship and the components of added resistance.

• 전력전자학회논문지
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• 제23권4호
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• pp.273-280
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• 2018

This paper proposes a zero-voltage-transition pulse-width modulation (PWM) DC-DC converter that uses a new active-snubber-cell. The converter main switch can be turned on and off with ZVS, while the snubber switch is turned on with ZCS and turned off with ZVS. Other semiconductor devices are operated under the soft-switching condition. Normal PWM control can be used, the proposed active-snubber-cell does not impose any additional voltage and current stresses. The active-snubber-cell is suitable for high-power applications due to its easy integration into interleaved converters. This paper discusses the operation of the converter, presents some design guidelines, and provides the results of an experiment with a 100 kHz and 1 kW prototype. A peak efficiency of 97.8% is recorded.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2001년도 추계 학술대회논문집
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• pp.84-90
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• 2001

Secondary instability of flow past a circular cylinder is examined using direct numerical simulation at Reynolds number 220 and 250. The higher-order finite difference scheme is employed for the spatial distributions along with the second order Adams-Bashforth and the first order backward-Euler time integration. In x-y plane, the convection term is applied by the 5th order upwind scheme, and the pressure and viscosity terms are applied by the 4th order central difference. In spanwise, Navier-Stokes equation is distributed using Spectral Method. The critical Reynolds number for this instability is found to be about Re=190. The secondary instability leads re three-dimensionality with a spanwise wavelength about 4 cylinder diameters at onset (A-mode). Results of three-dimensional effect in wake of a circular cylinder are represented with spanwise and streamwise vorticity contours as Reynolds numbers.

• 제어로봇시스템학회논문지
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• 제18권4호
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• pp.375-383
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• 2012

Together with the GPS-based approach, geolocation through mobile communication networks is a key technology for location-based service. Since the Mobile WiMAX system is considered as a candidate for fourth-generation mobile systems, it is important to investigate its location capability. The geolocation of Mobile WiMAX can be realized when the preamble symbols in the down-link channel are appropriately used for a TDOA (Time-Difference-of-Arrival) approach. However, the cellular structure of Mobile WiMAX inevitably generates co-channel interference, and it is difficult for the mobile terminal to acquire distance measurements from multiple base stations. Therefore, for geolocation via multilateration using the Mobile WiMAX network, it is very important to increase hearability. This paper proposes a geolocation method for Mobile WiMAX which employs interference cancellation and preamble signal overlapping for the enhancement of hearability. A novel interference cancellation strategy for complex-valued Mobile WiMAX signals is presented which has an iterative structure. Simulation results show that the proposed geolocation method provides the user's position with an accuracy of less than 20 m through the Mobile WiMAX cellular network if there is no multi-path or NLOS (None-Line-of-Sight).

• Interaction and multiscale mechanics
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• 제3권4호
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• pp.309-320
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• 2010

With taking the geometric nonlinearity of bridge structure into account, a framework is presented for predicting the dynamic responses of a long-span suspension bridge subjected to running train and turbulent wind. The nonlinear dynamic equations of the coupled train-bridge-wind system are established, and solved with the Newmark numerical integration and direct interactive method. The corresponding linear and nonlinear processes for solving the system equation are described, and the corresponding computer codes are written. The proposed framework is then applied to a schemed long-span suspension bridge with the main span of 1120 m. The whole histories of the train passing through the bridge under turbulent wind are simulated, and the dynamic responses of the bridge are obtained. The results demonstrate that the geometric nonlinearity does not influence the variation tendency of the bridge displacement histories, but the maximum responses will be changed obviously; the lateral displacement of bridge are more sensitive to the wind than the vertical ones; compared with wind velocity, train speed affects the vertical maximum responses a little more clearly.