• Clinical and Experimental Reproductive Medicine
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• 제41권1호
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• pp.1-8
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• 2014

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.

• KIEE International Transactions on Electrophysics and Applications
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• 제4C권2호
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• pp.45-50
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• 2004

The field utilization factor (equation omitted) (the mean electric field / the maximum electric field) of standard sphere gaps was calculated by the charge simulation method, taking into account the ground plane and shanks. n changes mainly with g/r and slightly with 1$_1$, 1$_2$ and 1, where D=2r is the sphere diameter, g is the gap length, 1$_1$ and 1$_2$, respectively, are the lengths of the upper and lower shank, and t is the shank diameter. Generally, (equation omitted) increases as 1$_1$,1$_2$ and t each becomes larger. IEC standard 60052(2002) limits t$\leq$0.2D 1$_1$$\geq$1D and prescribes A=1$_2$＋D＋g where A is the height of the spark point on the upper sphere. Therefore, (equation omitted) is the largest when A=9D and the smallest when A=3D. The simple equation of a straight line, (equation omitted)=1- (g/3r), can generally be used as a representative value of (equation omitted) for a wide variety of sphere diameters that are permitted by the IEC standard. The maximum electric field E$_{m}$ at sparkover of standard air gaps has also been calculated by the relation E$_{m}$=V/(equation omitted)g). E$_{m}$ describes a U-curve for g/r, up to the sphere diameter of 1 m. Moreover, for 1.5-m and 2-m diameters and especially .for negative polarity, sparkover voltages have been calculated by integration of the ionization index.index.

• Journal of Computing Science and Engineering
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• 제4권4호
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• pp.291-312
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• 2010

The last decades a large amount of research has been done in the genomics domain which has and is generating terabytes, if not exabytes, of information stored globally in a very fragmented way. Different databases use different ways of storing the same data, resulting in undesired redundancy and restrained information transfer. Adding to this, keeping the existing databases consistent and data integrity maintained is mainly left to human intervention which in turn is very costly, both in time and money as well as error prone. Identifying a fixed conceptual dictionary in the form of a conceptual model thus seems crucial. This paper presents an effort to integrate the mutational data from the established genomic data source HGMD into a conceptual model driven database HGDB, thereby providing useful lessons to improve the already existing conceptual model of the human genome.

• 한국결정성장학회지
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• 제14권4호
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• pp.151-154
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• 2004

Issues of ferroelectric high-density memories (>64 Mb) indispensable for upcoming ubiquitous era have been on the cell integration less than $0.1\;\mu\textrm{m}^2$ and reliabilities. Thus nanoscale control of microstructures of ferroelectric films with large switching polarization has been one of the issues to obtain the uniform electrical properties for realization of high-density memories. In this study the grain orientations and distributions of BT-based films by spin-on coatings were examined by FEG-SEM/EBSD. Ferroelectric domain characteristics by PFM were also performed to study the dependence of reliabilities on the grain orientations and distributions. It is believed that understandings of the nucleation and growth mechanisms of the a- or b-axis oriented films during the thermal processes such as RTA and furnace annealing affecting on grain orientation and uniformity could be possible based on our experimental results.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.1205-1210
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• 2004

The 4-20mA analog signal used in the various industrial fields to interface sensor in distributed process control has been replaced with relatively simple digital networks, called "fieldbus, and recently by Ethernet. Significant advances in Internet and computer technology have made it possible to develop an Internet based control, monitoring, and operation scheduling system for heating, ventilation and air-conditioning (HVAC) systems. The seamless integration of data networks with control networks allows access to any control point from anywhere. Field compatible field devices become so-called "smart" devices, capable of executing simple control, diagnostic and maintenance functions and providing bidirectional serial communication to higher level controller. The most important HVAC of BAS has received nationwide attention because of higher portion of more than 40% in building sector energy use and limited resources. This paper presents the Internet-based monitoring and control architecture and development of LonWorks control modules for AHU (air handling units) of HVAC in viewpoint of configuring BAS network. This article addresses issues in architecture section, electronics, embedded processors and software, and internet technologies.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 하계종합학술대회 논문집(2)
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• pp.399-402
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• 2004

This paper presents a system-on-chip prototype implementing a full integration for a portable digital audio system. The chip is composed of a audio processor block to implements audio decoding and voice compression or decompression software, a system control block including 8-bit MCU core and Memory Management Unit (MMU) a low power 16-bit ${\Sigma}{\Delta}$ CODEC, two DC-to-BC converter, and a flash memory controller. In order to support other audio algorithms except Mask ROM type's fixed codes, a novel 16-bit fixed-point DSP core with the program-download architecture is proposed. Funker, an efficient power management technique such as task-based clock management is implemented to reduce power consumption for portable application. The proposed chip has been fabricated with a 4 metal 0.25um CMOS technology and the chip area is about 7.1 mm ${\times}$ 7.1mm with 100mW power dissipation at 2.5V power supply.

• 한국가축번식학회지
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• 제23권4호
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• pp.381-391
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• 1999

During the last 20 years, transgenic animal technology has provided revolutionary new opportunities in many aspects of agriculture and biotechnology. Several gene delivery systems including pronuclear injection, retroviral vectors, sperm vectors, and somatic cell cloning have developed for making transgenic animals. In the future major improvements in transgenic animal generation will be mainly covered by somatic cell cloning technology. Many factors affecting integration frequency and expression of the transgenes should be overcome to facilitate the industrial applications of transgenic technology. Transgenic animal technology has settled down in some areas of the biotechnology, especially the mass production of valuable human proteins and xenotransplantation. In the 21st century animal biotechnology will further contribute to welfare of human being.

• International Journal of Quality Innovation
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• 제4권1호
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• pp.175-190
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• 2003

This paper discusses the determinants of green practices and incorporates some empirical findings from a recent study in the petrochemical sector in the Republic of Trinidad and Tobago. The study was comprised of a survey and follow-up interviews with senior executives who participated in the survey. Of fourteen companies involved, the findings affirmed that the investigation of accidents, provision of an emergency response, employee training, decreasing the production of wastes, and pre-treating wastes before disposal should be stressed. Government requirement, the economic reasons and public pressures were the driving forces of green practices. The five most important determinants identified include performance evaluation, financial justification, impacts on company, management leadership and operations integration. Implementing environmental management systems as a feasible approach to green practices in the petrochemical sector was explored. The findings provide guidance that helps organisations to accommodate the determinants of green practices into achieving sustainable environmental goals.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.838-843
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• 2005

Brain-derived neurotrophic factor (BDNF) is a small secretory protein and a member of the nerve growth factor (NGF) gene family. We cloned the flounder BDNF gene from a flounder brain cDNA library. The nucleotide sequence of the cloned gene showed an open reading frame (ORF) consisting of 810 bp, corresponding to 269 amino acid residues. The tissue distribution of flounder BDNF was determined by reverse transcription-polymerase chain reaction (RT-PCR) in brain, embryo, and muscle tissues. To express fBDNF using a eukaryotic expression system, we constructed the vector mpCTV-BDNF containing the fBDNF gene and transformed this vector into Chlorella ellipsoidea. Stable integration of introduced DNA was confirmed by PCR analysis of genomic DNA, and mRNA expression in C. ellipsoidae was confirmed by RT-PCR analysis.

• 전기학회논문지
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• 제56권4호
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• pp.768-772
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• 2007

Integration of RF front end module(FEM) into the antenna has been investigated in USPCS band (1.88GHz-1.99GHz). The FEM consists of input filter, power amplifier, coupler, power detector, bias switch and duplexer. The antenna was designed in planar inverted F antenna(PIFA) structure to implement it inside the handset. In order to avoid strong coupling between the antenna and FEM, a shielding ground layer was placed between them. The antenna size is 19mm by 10mm by 6mm under which FEM whose size is 8mm by 5mm by 1.5m locates. The antenna impedance was selected to match to FEM having better efficiency rather than gain since FEM has enough gain whose system spec is minimum of 20dB. The antenna patterns are shown with and without FEM.