• Title/Summary/Keyword: 4CBP

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Physicochemical Properties of the Exopolysaccharides Produced by Marine Bacterium Zoogloea sp. KCCM10036

  • Lim, Dong-Jung;Kim, Jong-Deog;Kim, Min-Yong;Yoo, Sang-Ho;Kong, Jai-Yul
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.979-984
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    • 2007
  • The physicochemical properties of the exopolysaccharide (EPS) produced by marine bacterium Zoogloea sp. KCCM10036 were investigated. Two types of isolated EPSs were shown to have average relative molecular masses $(M_r)\;of\;4.07{\times}10^6$ of CBP (cell-bound polysaccharide) and $3.43{\times}10^6$ of WSP (water-soluble polysaccharide), respectively. When the CBP was utilized as an emulsifier, it stabilized the emulsifier, for up to 148 h. Compared with other commercially available hydrocolloids such as xanthan gum, the Tween series, and Triton, the CBP showed much better emulsifying capability on a water-in-oil system. Phase separation occurred in the Tween series after 24 h, whereas the emulsion was better stabilized by the CBP. The CBP thus has potential as an emulsifying agent in commercial emulsions. The flocculating activity was also greatest at 0.01% (w/v) and decreased at higher concentrations than the optimized concentration of the WSP and CBP. The results also showed that both types of expolysaccharides from Zoogloea sp. had excellent flocculating activity.

A Study on the Call Admission Control with Overflow and Preemption at Adaptive Moving Boundary in Cellular Mobile Communications (셀룰러 이동통신망의 적응성 가변경계에서 Overflow와 Preemption을 갖는 호 접속제어 방안 연구)

  • 노희정
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.18 no.4
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    • pp.171-180
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    • 2004
  • CDP(handoff Call Dropping Probability) and CBP(new Call Blocking Probability) have been used as two important call level QoS parameters in cellular mobile communications. But, many methods to reduce CDP without considering CBP have been studied, and hand-off call priority scheme has been introduced. But the use of hand-off call priority scheme increases CBP and decreases channel utilization rate depending on the number of reserved channel for priority. In this paper, we propose a CAC(Call Admission Control) algorithm with overflow and preemption to solve the problem caused by considering CDP and CBP in calculation of the number of channel reserved. The problem is the increase of CDP as the traffic load increases. In our CAC algorithm, hand-off call is permitted to use(overflow) unreserved and unused channel if there is no reserved and unused channel, and new call is permitted to use(preemption) the channel overflowed by hand-off call if there is no unreserved and unused channel. This mechanism of calculation of the number of reserved channel and CAC algorithm is expected to increase channel utilization rate, and can be applied to media-based QoS provision in cellular mobile communications.

Development of Fusant Degrading Aniline and 4-chlorobiphenyl by Spheroplast Fusion between Pseudomonas sp. and Flavimonas oryzihabitans (Flavimonas oryzihabitans와 Pseudomonas sp.간 원형질체 융합에 의한 Aniline과 4-chlorobiphenyl 분해균주 개발)

  • 박형수;박용근;김무훈;고범준;조미영;김치경
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.259-266
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    • 2000
  • Spheroplast cell fusions were performed with Flavimonas oryzihabitans degrading aniline and Pseudomonas sp. degrading 4-chlorobiphenyl to develope the new fusant degrading aniline and 4-CBP and its characters were investigated. F. oryzihabitans was induced to antibiotic marker ($Cm^r$ by NTG treatment for the fusants selection. The results of spheroplast formation and regeneration frequencies of the strains treated with lysozyme-EDTA were 99% and 5.0~6.6%, respectively. Fusion products were treated with 40% (v/v) PEG 6000 and fusion frequency was $3.16{\times}10^{-4} $. The DNA content of fusant, F22 was approximately 2-fold compared with parents. The fusant was stable, and showed the mixed biochemical characteristics of the parent strains. F22 was similar to parent for cell growth pattern and degrading capacity on 5 mM aniline but cell growth rate of F22 was 1.5-fold higher than that of the parent on 10mM aniline. However 4-CBP degrading ability of F22 was slightly lower than that of parental strain.

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Dietary Supplementation of Citrus and Fermented Citrus By-product for Juvenile Red Seabream Pagrus major at Low Water Temperature (저수온기(13-15℃) 치어기 참돔(Pagrus major) 사료 내 비타민 C 대체제로써의 감귤착즙박 및 발효감귤착즙박의 이용 가능성)

  • Lee, Chorong;Kim, Youjeong;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.4
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    • pp.454-458
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    • 2015
  • This study compared the effects of dietary supplementation of citrus by-product (CBP) and CBP fermented with Bacillus subtilis (F-CBP) on growth performance, feed utilization, intestinal histology and innate immunity of red seabream Pagrus major with three commercial immune-boosting products. The six experimental diets were supplemented with L-ascorbyl-2-polyphosphate (LAPP; the control diet), CBP or F-CBP at a concentration of 100 mg vitamin C equivalent/kg diet or one of three commercial immune boosters. The Experimental diets were fed to triplicate groups of 17 fish (initial body weight, 116 g) for 8 weeks. The water temperature during the feeding trial was maintained at $13-15^{\circ}C$. Growth and feed utilization did not differ significantly among the six dietary treatments, nor did the phagocytic activity, superoxide dismutase or total immunoglobulin concentrations. However, myeloperoxidase activity was significantly higher in the CBP groups. For the intestinal histology, the intestine diameter, villi and enterocyte heights and number of goblet cells did not differ significantly among groups. Therefore, CBP or F-CBP can be used as a valuable eco-friendly byproduct in diets for fishes including red seabream to maintain their normal growth and health.

Production Conditions of Two Polysaccharides from Marine Bacterium Zoogloea sp. (해양세균 Zoogloea sp.로부터 두 종류의 polysaccharide 생산조건)

  • 장명웅;강양순
    • KSBB Journal
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    • v.10 no.5
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    • pp.518-524
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    • 1995
  • Marine bacterium, as a microbial source producing polysaccharides, was newly isolated from the eastern and western sea of Korea and was identified as Zoogloea sp. (KCCM 10036). It produced two different types of polysaccharides, especially: WSP (water-soluble polysaccharide) and CBP (cell-bound polysaccharide). The former was isolated from the supernatant of centrifuged broth by acetone precipitation, and the latter was isolated from the pellet by acetone and CPC (cetylpyrldinium chloride) precipitation. The productivity of polysaccharides were increased with the addition of promoting agents such as biotin, ampicillin and surfactant. After batch fermenting, the productivity of WSP and CBP were reached to maximum values of $9.Og/\ell$, $2.5g/\ell$ in the culture medium containing 1% of glucose as a carbon source.

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Divergence of the cbp Genes in 4-Chlorobiphenyl Catabolizing Bacteria (4-Chlorobiphenyl 분해 세균에서 cbp 유전자군의 상이성)

  • 윤덕중;한재진;김치경;김영수
    • Korean Journal of Microbiology
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    • v.30 no.1
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    • pp.53-59
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    • 1992
  • Four bacterial strains capable of catabolizing 4-chlorobiphen!;l (4CB) were isolated from the industrial waste waters. The bacterial isolates designated as PO$. P20, P27, and P1242. respectively, were examined for their catabolic activities. And in order to examine molecular homology of the 4CB catabolizing genes of these bacterial isolates. Southern hybridization was conducted with bphABC genes of P. p.srudoalculigrnrs KF707 as a DNA probe. The metabolites of 2-hydroxy-6-0x0-6-(4'-chlorophenyl)hexa-2 .4-dienoic acid and Cchlorobenzoate were detected to be produced by the isolatc:~ in the MM2 liquid cultures. But Cchlorobenzoate was further catabolized to produce 4.-hydroxybenzoate by DJ-12, P08. and P27. but not by P20 and P1242. As results of hybridization, homologous regions were commonly observed in Xhol fragments of 2.2 and 1.8 kb and in EcoRl fragment of 11 kb in the DJ- 12. P08, and P27 isolates. But in any restriction enzyme digests ot the P20 and PI242 isolates. homologous region was not detected. The cbp genes of the bactcria capable of catabolizing 4CB in nature could be divided into two groups by divergence<

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나노크기의 폭을 가진 우물 형태의 이중 발광층을 사용한 청색 유기발광소자의 색안정성과 색순도 향상 메카니즘

  • Go, Yo-Seop;Seo, Su-Yeol;Bang, Hyeon-Seong;Chu, Dong-Cheol;Kim, Tae-Hwan;Seo, Ji-Hyeon;Kim, Yeong-Gwan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.425-425
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    • 2010
  • 유기 발광 소자는 차세대 디스플레이 소자와 조명 광원으로서 많은 응용성 때문에 활발한 연구가 진행되고 있다. 하지만 청색 유기 발광 소자는 적색과 녹색 유기발광소자들에 비해 상대적으로 발광효율이 낮고 색 순도가 떨어지며 수명이 짧기 때문에 전색 유기발광소자를 구현하는데 문제가 있다. 이런 문제점을 해결하기 위하여 청색 유기 발광소자의 재료 개발, 다층 이종구조 및 형광/인광성 물질의 도핑에 대한 연구가 진행되고 있다. 이와 더불어 색안정성과 색순도가 향상된 진청색 고효율 청색 유기발광소자는 백색유기발광소자의 응용성 때문에 이에 대한 연구가 주목을 받고 있다. 본 연구에서는 청색 유기 발광 소자의 발광효율을 높이고 색안정성과 색순도를 향상하기 위해 4,4'-Bis (2,2'-diphenyl-ethen-1-yl)biphenyl (DPVBi) 와 4,4'-Bis(carbazol-9-yl) biphenyl (CBP)로 구성된 나노크기의 폭을 가진 우물 형태의 이중 발광층 구조를 사용한 청색 유기발광소자를 제작하였다. 제작된 청색유기발광소자의 전기적 성질과 광학적 성질을 조사하여 색안정성 및 색순도 향상 메카니즘을 관찰하였다. DPVBi/CBP 이중 발광층을 가지는 청색 유기발광소자에서 CBP의 HOMO 에너지 준위의 값이 3.2 eV로 매우 크기 때문에 정공을 막는 정공 장벽층의 역할을 하게 되어 정공이 발광층에 머무르게 된다. 또한 DPVBi의 LUMO 값의 크기 5.8 eV, CBP의 LUMO 값의 크기는 6.3 eV이므로 상대적으로 CBP의 전자에 대한 주입장벽이 크기 때문에 발광층에 머무르는 전자의 양이 증가된다. 청색 발광층에 사용된 이중 발광층은 단일 발광층에 비해 더 많은 전자와 정공이 존재하기 때문에 전자-정공 재결합 확률을 높였으며 재결합 영역이 발광층 중심의 이중발광층 계면으로 이동하여 발광 영역이 국소화되어 전압변화에 따른 색의 변화가 적고 색순도가 더욱 향상되었다.

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Catabolic Degradation of 4-Chlorobiphenyl by Pseudomonas sp. DJ-12 via Consecutive Reaction of meta-Cleavage and Hydrolytic Dechlorination

  • Chae, Jong-Chan;Kim, Eunheui;Park, Sang-Ho;Kim, Chi-Kyung
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.6
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    • pp.449-455
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    • 2000
  • Pseudomonas sp. strain DJ-12 is a bacterial isolate capable of degrading 4-chlorobiphenyl (4CBP) as a carbon and energy source. The catabolic degradation of 4CBP by the strain DJ-12 was studied along with the genetic organization of the genes responsible for the crucial steps of the catabolic degradation. The catabolic pathway was characterized as being conducted by consecutive reactions of the meta-cleavage of 4CBP, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, and meta-cleavage of protocatechuate. The pcbC gene responsible for the meta-cleavage of 4CBP only showed a 30 to 40% homology in its deduced amino acid sequence compared to those of the corresponding genes from other strains. The amino acid sequence of 4CBA-CoA dechlorinase showed an 86% homology with that of Pseudomonas sp. CBS3, yet only a 50% homology with that of Arthrobacter spp. However, the fcb genes for the hydrolytic dechlorination of 4CBA in Pseudomonas sp. DJ-12 showed an uniquely different organization from those of CBS3 and other reported strains. Accordingly, these results indicate that strain DJ-12 can degrade 4CBA completely via meta-cleavage and hydrolytic dechlorination using enzymes that are uniquely different in their amino acid sequences from those of other bacterial strains with the same degradation activities.

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The Optimal Composition of Cold Bonded Pellet for Recycling EAF Dust Directly to the Furnace

  • Lee, Kwang-Keun;Kim, Tai-Dong
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.370-374
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    • 2001
  • The degree of iron recovery from dust agglomerates was investigated experimentally to determine the optimum mixing ratio of coke in cold bonded pellet(CBP) which is fed into electric arc furnace(EAE) in the minimill plant. From the XRD analysis for EAF dust, magnetite(Fe$_3$O$_4$) and franklinite(ZnFe$_2$O$_4$) was identified as major components. Maximum iron recovery was obtained for the solid carbon content of approximately 18 weight percent. From plant trials of CBP composed of this optimal mixing condition, it was observed that electric power consumption and sulfur content increased with increasing the quantity of CBP.

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Dosimetric Evaluation of Amplitude-based Respiratory Gating for Delivery of Volumetric Modulated Arc Therapy (진폭 기반 호흡연동 체적변조회전방사선치료의 선량학적 평가)

  • Lee, Chang Yeol;Kim, Woo Chul;Kim, Hun Jeong;Park, Jeong Hoon;Min, Chul Kee;Shin, Dong Oh;Choi, Sang Hyoun;Park, Seungwoo;Huh, Hyun Do
    • Progress in Medical Physics
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    • v.26 no.3
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    • pp.127-136
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    • 2015
  • The purpose of this study is to perform a dosimetric evaluation of amplitude-based respiratory gating for the delivery of volumetric modulated arc therapy (VMAT). We selected two types of breathing patterns, subjectively among patients with respiratory-gated treatment log files. For patients that showed consistent breathing patterns (CBP) relative to the 4D CT respiration patterns, the variability of the breath-holding position during treatment was observed within the thresholds. However, patients with inconsistent breathing patterns (IBP) show differences relative to those with CBP. The relative isodose distribution was evaluated using an EBT3 film by comparing gated delivery to static delivery, and an absolute dose measurement was performed with a $0.6cm^3$ Farmer-type ion chamber. The passing rate percentages under the 3%/3 mm gamma analysis for Patients 1, 2 and 3 were respectively 93.18%, 91.16%, and 95.46% for CBP, and 66.77%, 48.79%, and 40.36% for IBP. Under the more stringent criteria of 2%/2 mm, passing rates for Patients 1, 2 and 3 were respectively 73.05%, 67.14%, and 86.85% for CBP, and 46.53%, 32.73%, and 36.51% for IBP. The ion chamber measurements were within 3.5%, on average, of those calculated by the TPS and within 2.0%, on average, when compared to the static-point dose measurements for all cases of CBP. Inconsistent breathing patterns between 4D CT simulation and treatment may cause considerable dosimetric differences. Therefore, patient training is important to maintain consistent breathing amplitude during CT scan acquisition and treatment delivery.