• 환경생물
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• 제22권
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• pp.71-77
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• 2004

광양만 및 주변 하천에 존재하는 페놀류 화합물과 sterol 화합물의 분포특성을 알아보기 위하여 alkylphenols (8종), chlorophenols (2종), bisphenol A (1종), coprostanols (2종) 및 cholesterol (1종) 등 화합물들을 분석대상물질로 선정하여 표층수와 표층퇴적물 시료를 분석하였다. 모든 분석대상물질 중에서 nonylphenol과 dihydrocholesterol 화합물이 가장 높은 검출빈도를 보였으며 일부 하천퇴적물 시료에서 t-octylphenol, bisphenol A, coprostanols이 검출되었다. 분석대상물질은 하천시료에서 비교적 높게 검출되었으며, 또한 광양만 내해에서 외해보다 높게 검출되었다. 표층해수와 표층해양퇴적 물에서의 nonylphenol농도는 각각 4.0∼74.0 ng $l^{-1}$ 및 3.1∼74.3 ng $g^{-1}$ dry wt.이었다. 해양표층퇴적물에 의한 nonylphenol 평균 농축배수(Log $K_{oc}$ )는 4.8이었다. 하천표층퇴적물에서의 nonylphenol농도는 4.6∼808.6 ng $g^{-1}$ dry wt.이었으며 dihydrocholesterol 농도는 78.4∼1133.6 ng $g^{-1}$ dry wt.이었다 공단지 역을 경유하는 하천시료에서는 상대적으로 높게 nonylphenol이 검출되었으며 주민 거주지역을 경유하는 하천퇴적물에서는 dihydrocholesterol 화합물이 상대적으로 높은 노출농도를 보이였다. 섬진강 표층수에서의 nonylphenol 농도는 염분도의 증가에 따라 낮아지는 경향을 보였다.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1740-1746
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• 2006

Enrichment techniques led to the isolation of a Pseudomonas sp. strain P2 from municipal waste-contaminated soil sample, which could utilize different isomers of a commercial mixture of 4-nonylphenol when grown in the presence of phenol. The isolate was identified as Pseudomonas sp., based on the morphological, nutritional, and biochemical characteristics and 16S rDNA sequence analysis. The ${\beta}$-ketoadipate pathway was found to be involved in the degradation of phenol by Pseudomonas sp. strain P2. Gas chromatography-mass spectrometric analysis of the culture media indicated degradation of various major isomers of 4-nonylphenol in the range of 29-50%. However, the selected ion monitoring mode of analysis of biodegraded products of 4-nonylphenol indicated the absence of any aromatic compounds other than those of the isomers of 4-nonylphenol. Moreover, Pseudomonas sp. strain P2 was incapable of utilizing various alkanes individually as sole carbon source, whereas the degradation of 4-nonylphenol was observed only when the test organism was induced with phenol, suggesting that the degradation of 4-nonylphenol was possibly initiated from the phenolic moiety of the molecule, but not from the alkyl side-chain.

• 한국환경과학회지
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• 제2권4호
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• pp.271-278
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• 1993

Nonylphenol ethoxylates-30을 분해할 수 있는 Pseudomonas sp.를 분리, 동정하였다. Nonylphenol ethoxylates-30 의 최적분해조건은 탄소원으로 nonylphenol ethoxylates-30 1.0 g/ι, 질소원으로 ammonium nitrate 0.02 g/ι, pH 7.5, 30였다. 최적분해 조건에서 nonylphenol ethoxylates-30은 배양 30시간 후 89%가 분해되었다. 최적분해조건에서 nonylphenol ethoxylates-30의 초기농도가 각각 100 ppm, 500 ppm, 1000 ppm, 5000 ppm일 때 시간에 따른 분해율을 수식으로 설명할 수 있는 가장 적합한 분해 kinetics는 각각 first order model, Monod no growth model, Monod with growth model로 나타났다.

• 생태와환경
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• 제41권4호
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• pp.519-529
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• 2008

Recent studies reveal one of the representative endocrine disrupters of nonylphenol affects on the composition of a planktonic community. Since nonylphenol is sometimes discharged into eutrophic waters, we monitored planktonic community composition of a eutrophic pond after receiving nonylphenol when cyanobacterium Microcystis aeruginosa mainly dominated. The experiment was carried out two times using small-scale microcosms in a laboratory. In both two experiments, ciliate abundances significantly decreased when nonylphenol was added. On the seventh day, the ciliate abundances in $10{\mu}g\;L^{-1}$ added treatments decreased by 36.9% in the first experiment and 33.6% in the second, when compared to the control. The response of other planktonic groups was less obvious to nonylphenol addition. In particular, in the first experiment, Chl. b/Chl. $\alpha$ and Chl. c/Chl. $\alpha$ significantly increased with the addition of nonylphenol, while total Chl. $\alpha$ concentration did not change. Indeed, bacillariophyceae and chlorophyceae abundances tended to increase with nonylphenol dosing. From these results, we tentatively hypothesized that nonylphenolloading positively affects on abundances of edible phytoplankton such as Scenedesmus spp. and diatoms by releasing from grazing pressure due to decrease in ciliate abundances. The present study emphasizes that the indirect effect of endocrine disrupters should be paid more attention when freshwater resources are polluted by them.

• 생태와환경
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• 제41권4호
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• pp.449-456
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• 2008

Recent studies reveal that the endocrine disrupter nonylphenol can also influence the growth of planktonic organisms. To clarify the effect of nonylphenol on the whole planktonic community, we monitored planktonic abundances after addition of nonylphenol using small-scale microcosms in a laboratory. Nonylphenol was added at final concentrations of 1.25 and $2.5{\mu}g\;L^{-1}$, close to the EC50 for the growth of the rotifer, Brachionus calyciflorus. Chlorophyll $\alpha$ concentration increased significantly between 2 to 5 days after nonylphenol treatment compared to the control. The abundance of the predominant phytoplankton, Stephanodiscus hantzschii, followed the same pattern as chlorophyll a concentration. While there was no negative effect on the abundance of ciliates and rotifers, crustacean zooplankton abundance was higher in nonylphenol treatments. Although the relationship did not reach significance, the growth rate of rotifers tended to decline with increasing nonylphenol dosing. It is likely that the decreased rotifer grazing on S. hantzschii caused significant increase in their abundance. This study emphasizes the importance of considering indirect effects of environmental pollutants when predicting the response of biological community to toxicant exposure.

• Environmental Analysis Health and Toxicology
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• 제20권4호통권51호
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• pp.279-286
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• 2005

Earthworm (Eisenia fetida) acute toxicity test was carried out and ecological risk assessment in soil was performed with national monitoring data. 14 day - $LC_{50}$ of nonylphenol and bisphenol A were 288.1 mg/kg and 90.1 mg/kg, respectively. And NOECs of nonylphenol and bisphenol A were 250 mg/kg and 50 mg/kg, respectively. Significant weight decrement was appeared at 70 mg/kg of bisphenol A, however, nonylphenol at concentrations tested did not severe adverse effect on the weight decrement. The environmental monitoring has been carrying out by NIER since 1999. Exposure levels of nonylphenol in soil were ND$\sim$10.55 $\mu$g/kg and those of bisphenol A were ND$\sim$15.50$\mu$g/kg in National Monitoring data which had been performed from 2000 to 2004. The measured soil exposure level was applied to evaluate the environmental risk assessment. The values of PNEC for bisphenol A and nonylphenol were determined as 0.5 mg/kg and 2.5 mg/kg, respectively using the safety factors which were suggested in EU and OECD. The values of HQ (PEC/PNEC) were determined to be below I for bisphenol A and nonylphenol when the maximum exposure levels for bispheol A (15.50$\mu$g/kg) and nonylphenol (10.55$\mu$g/kg) were applied. Conclusively, the environmental risk assessment of bisphenol A and nonylphenol was not critical in soil.

• 환경생물
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• 제33권4호
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• pp.468-475
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• 2015

노닐페놀에 물벼룩을 노출하여 급성독성수치를 산출하고 만성독성을 조사하여 생존, 생식능, 성장에 미치는 영향을 관찰하였다. 그 결과, 노닐페놀에 노출된 물벼룩의 급성독성 수치 $EC_{50}$은 용량의존적인 증가를 보였으며, 물벼룩의 일령 증가에 의한 $EC_{50}$의 증가 폭은 크지 않았다. 만성독성시험에 의한 생존률은 최고농도인 $10{\mu}g\;L^{-1}$에서 90%로 관찰되어 대조군과 유의한 차이를 보이지 않았으나, 대조군과 비교하여 고농도에서 총 출산한 새끼수, 출산횟수가 감소하며, 첫 출산까지 걸리는 시간이 늘어나는 경향이 관찰되었고 NOEC와 LOEC는 각각 $3.2{\mu}g\;L^{-1}$, $5.6{\mu}g\;L^{-1}$로 산출되었다. 또한 체장 측정 결과 대조군에 비해 노닐페놀 노출군에서 농도가 증가할수록 성장이 저해되는 양상이 관찰되었다. 이러한 결과는 노닐페놀이 물벼룩의 생식을 저해시키는 물질로 작용했음을 알 수 있다.

• 환경생물
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• 제25권1호
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• pp.66-71
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• 2007

실내에서 사육된 C. riparius를 대상으로 내분비계 교란물질인 BPA와 4-nonylphenol을 처리하여 형태적인 변화를 살펴보았다. 약제에 노출된 개체의 하순기절은 부드러워지거나(smooth) 손실(loss)이 가장 많았다. 처리물질에 따른 기형의 정도를 살펴보면, BPA는 $31{\sim}90%$, 4-nonlyphenol은 $40{\sim}80%$의 범위를 보였다. BPA는 처리 농도가 증가할수록 기형도 증가하였으나 4-nonylphenol은 노출농도 증가와 기형발생의 비례적으로 나타나지 않았다. 처리 물질별 하순기절의 기형 부위를 살펴보면, MIX타입의 기형이 가장 많이 나타났으며 그 다음으로 LT, MLT 타입의 기형이 나타났다. MLT에서는 부드러워지는 형태가 가장 많았고 LT에서는 teeth가 손실되는 loss 타입의 기형이 가장 많이 나타났다. 또한 LT에서는 부드러워지는 마모와 손실이 복합적인 타입이 그 다음으로 빈번하였다. MIX는 부드러워지거나 손실 타입의 기형이 가장 다수 관찰되었다.

• 공업화학
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• 제5권1호
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• pp.121-126
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• 1994

초산의 반응추출에 미치는 영향을 규명하기 위하여 추출제로 2급과 3급 아민 및 용매화 추출제, 용매로 n-butylacetate, modifier로 4-nonylphenol, TBP 및 isodecanol을 이용하였다. 그외에 수용액상의 pH와 온도의 영향을 연구하였다. 실험결과 초산의 추출에서 3급 아민인 tril-n-octyl과 tri-n-decylamine의 50% 혼합 추출제인 MODA가 추출도 및 선택도에서 가장 좋았다. Modifier로서는 4-nonylphenol이 우수하였다. 이외에도 수용액의 pH와 추출계의 온도가 낮을수록 추출도가 높았다.

• 한국환경과학회지
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• 제23권2호
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• pp.313-322
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• 2014

To investigate the effects of octylphenol and nonylphenol on the reproduction of the striped field mouse, the mice were subcutaneousely injected with 4-t-octylphenol 800 mg/kg (OP800), nonylphenol 900 mg/kg (NP900) or OP800 + NP900 (OPNP), respectively, in two times a week for 2 months. As the results, there were no differences between the control and the compound-treated groups in the body weight in both sexes, and the GSI (gonadosomatic index) and SVI (seminal vesicles index) in the adult males. But histological abnormalities of the reproductive organs in the mice seems to be related to the compound. This suggestion is supported by the facts that most mice treated with the compounds had only a small number of spermatozoa in the shrunken epididymal tubules. In addition, the shrunken reproductive organ in one mouse treated with the NP, suggests that the abnormalities in the wild striped field mice might be induced by the compound. Furthermore, it is indicated that the compounds must be toxicants to inhibit pregnancy, judging from the fact that all the mice treated with the compounds had failed to deliver.