• Title/Summary/Keyword: 4-hydroxyproline

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Studies on the Chemical Composition of Grape Juices in Korea (한국산(韓國産) 포도의 과즙성분(果汁成分)에 관(關)한 연구(硏究))

  • Kim, Sung-Yeol;Choi, Woo-Young;Kang, Jin-Hyung
    • Korean Journal of Food Science and Technology
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    • v.2 no.1
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    • pp.72-80
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    • 1970
  • The chemical composition of the of grape juices produced in Korea in 1969 were analyzed and the amino acids and sugars in these juices were detected by means of paper partition chromatography. The results are summarized as follows: 1. Less sugar and amino-N and more acid were contained in Korean grape juices than those of foreign's, and tannin content was not so different as foreign's. 2. More sugar, amino-N and tannin and less acid were contained in the juices of Campbell Early obtained in Anyang district than in Taejon and Pohang districts. 3. Twenty-one amino acids detected in grape juices were distributed as following frequency. Aspartic acid, serine, glycine, asparagine, lysine, arginine, threonine, alanine ${\gamma}-amino$ butyric acid, valine, leucine, proline (in 11 varieties), glutamine, tyrosine(10), cystine(9), glutamic acid, Hydroxyproline(8), isoleucine(4), phenylalanine, unknown(3). 4. Alanine was mostly abundant in all varieties and ${\gamma}-amino$ butyric acid was next and the decreasing order were arginine, valine, leucine, proline, glutamine, threonine, etc. in the amount. 5. Number of amino acid detected in grape juices of each varieties were 20 sorts in Delaware(Anyang), 19 in Black Hamburg, and Schuyler, 18 in Campbell Early(three districts), Delaware(seedless, Taejon) and Alden, 17 in Niagara, 16 in Muscat Hamburg, and 15 in Golden Muscat. 6. Number of essential amino acids contained in Delaware(Anyang) and Black Hamburg were 6 sorts and in Campbell Early (Anyang), Niagara and Muscat Hamburg were 5 and in others 4. 7. The same number of amino acid were detected in the juices of Campbell Early obtained from three districts, but hydroxyproline was detected in that of Anyang only, while isoleucine was appeared in those of Taejon and Pohang. 8. Glucose, fructose were detected in all grape juices.

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Proteolytic Conditions for the Hydrolysate of Flounder Skin Gelatin (효소에 의한 가자미피 젤라틴 가수분해물의 제조 조건)

  • 강태중;양현필;김세권;송대진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.4
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    • pp.398-406
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    • 1992
  • In order to develop a new flavourant using the fish skin gelatin, the proteolytic renditions for the gelatin hydrolysate of the alkali (B-type) and Alcalase (E-type) pretreated flounder (Limanda aspera) skin gelatin were investigated, and some physical properties, molecular weight and amino acid compositions of the hydrolysates were, also, compared with each other. The proteolytic conditions of the gelatins (B-type and E-type) by trypsin were as follows : reaction temperature, 55$^{\circ}C$ : pH, 9.0 : enzyme concentration, 0.1% : re-action time, 4hrs for B-type and 1 hr for E-type. The degrees of hydrolysis of the B-type and E-type gelatin un-der the renditions stated above were 63% and 82%, respectively. The rnajor molecular weights of the hydrolysates were 15,000 dalton for B-type and 12,400 dalton for E-type. Among the amino acids in the hydrolysates, glycine, alanine, proline, hydroxyproline and serine having a sweet taste were responsible for 57% of the total amino acid. But valine, leucine, phenylalanine, tyrosine, methionine, arginine and histidine having a bitter taste were only 18%.

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Inhibition of Prolyl 4-Hydroxylase by Oxaproline Tetrapeptides In Vitro and Mass Analysis for the Enzymatic Reaction Products

  • Moon Hong-sik;Begley Tedhg P.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.1
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    • pp.61-64
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    • 2000
  • A series of 5-oxaproline peptide derivatives was synthesized and evaluated for its ability to inhibit the prolyl 4-hydroxylase in vitro. Structure-activity studies show that the 5-oxaproline sequences, prepared by the 1,3-dipolar cycloaddition of the C-methoxycarbonyl-N-mannosyl nitrone in the presence of the ethylene, are more active than the corresponding proline derivatives. Prolyl 4-hydroxylase belongs to a family of $Fe^{2+}-dependent$ dioxygenase, which catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in -Gly-Xaa-Pro-Gly- of procollagen chains. In this paper we discover the more selective N-Cbz-Gly-Phe-Pro-Gly-OEt $(K_m\;=\;520\;{\mu}M)$ sequences which are showed stronger binding than others in vitro. Therefore, we set out to investigate constrained tetrapeptide that was designed to mimic the proline structure of pep tides for the development of prolyl 4-hydroxylase inhibitor. From this result, we found that the most potent inhibitor is N-Dansyl-Gly-Phe-5-oxaPro-Gly-OEt $(K_i\;=\;1.6\;{\mu}M)$. This has prompted attempts to develop drugs which inhibit collagen synthesis. Prolyl 4-hydroxylase would seem a particularly suitable target for antifibrotic therapy.

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Pharmacological activity of extracts Artermisia iwayomogi : liver regeneration and chronic hepatotoxicity

  • Lee, Soon-Bok;Cheol Jeong;Jeong, Seong-Hak;Lee, Sun-Mee;Cho, Tai-Soon
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.200-200
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    • 1996
  • 간재생 및 만성 간질환모델에 대한 인진호 추출분획의 간장 약효검색 방법 1. 간세포 재생능력에 대한 효과 : SD계 수컷 흰쥐(체중 300-350g)를 마취하에서 복부정중선을 따라 개복후 간의 중앙엽 및 좌측엽을 절제하여 봉합하고 수술조작을 끝낸 추 4시간, 익일부터 7일간 오후 5시에 약물을 경구투여하고 마지막투여 후 20시간 절식, 부검하여 간재생률 및 BSP 정체율을 측정하였다. 2. $CCl_4$ 만성간장해 모델에 대한 효자 SD계 수컷 흰쥐(체중 200-250g)에 $CCl_4$ : olive oil 40%용액을 0.5 $m\ell$/kg b.wt. 용량으로 6일간 결구투여 한 후 7일째부터 매일 시험약물을 $CCl_4$ 혼합액 투여 4시간전 및 4시간후에 2회 경구투여하였으며 이 조작을 8일간 하고 마지막 투여 24시간 후에 부검하여 혈액내 ALT 및 AST치, 간조직내 total protein, triglyceride 및 hydroxyproline 양을 측정하였으며, 간중량도 측정하였다. 3. 약물투여 음성대조 : 1% CMC-Na 용액(10$m\ell$/kg b.wt.) 양성대조 : silymarin(25mg/kg), DDB(37.5mg/kg) 인진호추출분획 : KP(180mg/kg), PS-1(300mg/kg), PS-2(300mg/kg), EE (500mg/kg ), HH(640mg/kg), BE(1500mg/kg)

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Tungtungmadic Acid Isolated from Salicornia herbacea Suppresses the Progress of Carbon Tetrachloride-induced Hepatic Fibrosis in Mice

  • Chung, Young-Chul;Choi, Jae-Ho;Oh, Kyo-Nyeo;Chun, Hyo-Kon;Jeong, Hye-Gwang
    • Toxicological Research
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    • v.22 no.3
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    • pp.267-273
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    • 2006
  • Tungtungmadic acid(3-caffeoyl, 4-dihydrocaffeoyl quinic acid: CDCQ) is a new chlorogenic acid derivative isolated from the Salicornia herbacea. The suppressive effects of CDCQ on the progress of acute carbon tetrachloride($CCl_4$)-induced hepatic fibrosis were investigated in mice. CDCQ significantly suppressed $CCl_4$-induced hepatic necrosis and inflammation, as determined by serum enzymatic activities of alanine and aspartate aminotransferase and serum TNF-$\alpha$ levels in a dose-dependent manner. In addition, increased hepatic lipid peroxidation and fibrosis after acute $CCl_4$ treatment were suppressed by the administration of CDCQ. CDCQ also significantly prevented the elevation of hepatic hydroxyproline and collagen content and ${\alpha}$-smooth muscle actin(${\alpha}$-SMA) expression in the liver of $CCl_4$-intoxicated mice. These results suggest that the suppressive effects of CDCQ against the acute $CCl_4$-induced hepatic fibrosis possibly related to its ability to block both hepatic inflammation and the activation of hepatic stellate cells.

Effect of Solanum lyratum Extract on Dimethylniatrosamine-Induced Liver Damage in Rats (Dimethylnitrosamine에 의해 유도된 흰쥐 간독성에 대한 배풍등 추출물의 보호효과)

  • Shin, Mi-Ok;Park, Jong-Hee;Moon, Jeon-Ok
    • Korean Journal of Pharmacognosy
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    • v.34 no.1 s.132
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    • pp.60-64
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    • 2003
  • Solanum lyratum(Solanaceae) has been used as a traditional analgesic, antipyretic and hepatoprotective agents in Korea. In this study, we investigated the hepatoprotective effect of ethylacetate extract of Solanum lyratum (SL) on the dimethylnitrosamine (DMN)-induced liver damage in rats. Oral administration of SL (150, 300 mg/kg daily for 4 weeks) into the DMN-treated rats remarkably prevented the elevation of serum alanine transaminase, aspartate transaminase and alkaline phosphatase levels. SL also increased serum protein level and reduced the hepatic level of malondialdehyde in DMN-treated rats. Furthermore, DMN-induced elevation of hydroxyproline content was reduced by the treatment of SL. In conclusion, these results demonstrated that SL exhibited in vivo hepatoprotective effect against DMN-induced liver injury, and suggest that SL may be useful in the prevention of liver damage.

General Components and Sensory Evaluation of Hot Water Extract from Liriopis Tuber (맥문동 열수 추출물의 일반성분과 관능평가)

  • 김순동;구연수;이인자;김일두;윤광섭
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.1
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    • pp.20-24
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    • 2001
  • This study was conducted to analyze the general components and to investigate sensory evaluation of hot water extract prepared by boiling at 10$0^{\circ}C$ for 3 hr with 4-fold water. The contents of total soluble soild, non-reducing sugar, total saponin, ash and total protein were 15.95%, 6.54%, 1.735, 0.33% and 0.40%, respectively. The contents of succinic, malic and acetic acid in the extracts were 111.48 mg%, 23.67mg% and 18.36mg%, respectively. The major free amino acids and minerlas of the extract were hydroxyproline 1,290.0 $\mu\textrm{g}$%, glutamic acid 456.2$\mu\textrm{g}$% and potassium 151.35 mg%, respectively. Bitter taste in the extract was not observed, whereas sweet, sour and astringent taste were observed.

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EFFECTS OF TEETH DEVELOPMENT ON THE COLLAGEN AND MINERALS IN RAT DENTIN (치아발육에 있어서 Collagen 및 무기질 변동에 미치는 영향)

  • Jeong, Tae-Yeong
    • The Journal of the Korean dental association
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    • v.13 no.6
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    • pp.563-567
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    • 1975
  • This present investigation was concerned with the content of collagen and minerals of incisor and molar dentin in rats of differednt ages. The results obtained were summarized as follows. 1. There was no remarked change in the content of collagen-hydroxyproline of incisor and molar dentin according to days after delivery. 2. The Ca content in incisor dentin rose from approximately 15.4% of the dry weight in 7 days after delivery to 21.1% in 22 days while that in molar dentin from 17.6% in 7 days to 19.7% in 22 days. 3. The P content in molar dentin rose from 8.5% in 7 days after delivery to 11.65% in 22 days. while in incisor rose from 8.05% in 7 days to 11.85 in 22 day after delivery.

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Dose-dependent antifibrotic effect of G009 on experimental hepatic cirrhosis

  • Park, Eun-Jeon;Kim, Su-Ung;Lee, Seung-Yong;Kim, Jaebaek;Sohn, Dong-Hwan
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.110-110
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    • 1995
  • This study was carried out to investigate the dose dependent antifibrotic effects of G009, the water-soluble fraction of polysaccharide extracted from Ganoderma lucidum. The experimental hepatic cirrhosis was induced by bile duct ligation/scission (BDL/S) in rats. BDL/S rats in each group were dosed 0.5, 2, 5 or 10mg/rat/day orally for 4 weeks after the operation. Antifibrotic effects were evaluated by serum biochemical values, hydroxyproline contents, and light microscopical histology.

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Studies on Free Amino Acids Composition of Salted Internal Organs of Several Fishes (어류내장(魚類內臟) 숙성(熟成)젓의 Free Amino Acids 조성(組成)에 관(關)한 연구(硏究))

  • Shin, Dong-Wha;Kim, Hyong-Soo
    • Applied Biological Chemistry
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    • v.9
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    • pp.83-90
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    • 1968
  • In order to elucidate the differences among the parts of internal organs of salted fishes (Chang Zut), five parts of the organs were examined for their chemical constituents including amino acids. The moisture content varied from 59.8% to 72.8% Crude protein; 7.13% to 11.19%, Crude ash; to 16.1%, 24.8%, Sodium chloride; 15.3% to 22.4% and ash; 0.8% to 2.2%. Seven essential amino acids including leucine, isoleucine, phenylalanine, tryptophan, threonine, lysine and methionine except valine were existed in the every part of the organ. The contents of tyrosine and hydroxyproline were relatively high, on the other hand, tryptophan was trace amount in the every parts.

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