• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.133-140
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• 2017

Conflicting evidence has been obtained regarding whether transient receptor potential cation channels (TRPC) are store-operated channels (SOCs) or receptor-operated channels (ROCs). Moreover, the Ca/Na permeability ratio differs depending on whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. A TRPC4 specific activator, (-)-englerin A, induced both current and calcium fluorescence with the similar time course. Muscarinic receptor stimulator, carbachol, also induced both current and calcium fluorescence with the similar time course. By forming heteromers with TRPC4, TRPC1 significantly reduced the inward current with outward rectifying I-V curve, which also caused the decrease of calcium fluorescence intensity. These results suggest that GCaMP6s attached to TRPC4 can detect slight calcium changes near TRPC4 channels. Consequently, TRPC4-GCaMP6s can be a useful tool for testing the calcium permeability of TRPC4 channels.

• ALGAE
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• 제36권4호
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• Korean Journal of Acupuncture
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• 제28권3호
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• pp.85-98
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• 2011

Objectives : The aim of this thesis is to examine the effect of acupuncture treatment at the GB37 on normal humans by using the power spectral analysis of the EEG. Methods : EEG (Electroencephalogram) power spectrum exhibits site-specific and state-related differences in specific frequency bands. In this thesis, the power spectrum was measured by the complexity. the 32 channels EEG study was carried out in the 13 subjects (12 males ; age=22.58 years old, 1 females ; 22 years old). Results : In the ${\alpha}$ (alpha) band, the power values at F7, F3, F4, F8, FTC2, C4, T4, CP1, CP2, TCP2, TT2, Pz, P4, Po1, Po2, O1, Oz, O2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the ${\beta}$ (beta) band, the power values at Cz, C4, T4, Tcp1, T6, Po1, O1, Oz, O2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the ${\delta}$(delta) band, the power values at Fp1, TT2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the $\theta$ (theta) band, the power values at Fp1, F8, FTC2, Pz channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. Conclusions : This results suggest that the acupuncture treatment at the GB37 significantly mostly change the power spectrum value on the alpha (18 channels), beta (9 channels) bands.

• BMB Reports
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• 제48권1호
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• pp.1-5
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• 2015

Transient Receptor Potential, Melastatin-related, member 4 (TRPM4) channels are $Ca^{2+}$-activated $Ca^{2+}$-impermeable cation channels. These channels are expressed in various types of mammalian tissues including the brain and are implicated in many diverse physiological and pathophysiological conditions. In the past several years, the trafficking processes and regulatory mechanism of these channels and their interacting proteins have been uncovered. Here in this minireview, we summarize the current understanding of the trafficking mechanism of TRPM4 channels on the plasma membrane as well as heteromeric complex formation via protein interactions. We also describe physiological implications of protein-TRPM4 interactions and suggest TRPM4 channels as therapeutic targets in many related diseases.

• The Korean Journal of Physiology and Pharmacology
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• 제4권6호
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• pp.427-437
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• 2000

The early studies of cardiac and smooth muscle cells provided evidence for two different calcium channels, the L-type (also called high-voltage activated [HVA]) and T-type (low-voltage activated [LVA]). These calcium channels provided calcium for muscle contractions and pace-making activities. As might be expected, the number of different calcium channels increased when researchers studied neurons and the identification of the neuronal calcium channels has proven to be much more difficult than with the muscle calcium channels. There are two reasons for this difficulty; (1) a larger number of different calcium channels in neurons and (2) many of the different calcium channels have similar kinetic properties. This review uses the N-type calcium channel to illustrate the difficulties in identifying and characterizing calcium channels in neurons. It shows that the discovery of toxins that can specifically block single calcium channel types has made it possible to easily and rapidly discern the physiological roles of the different calcium channels in the neuron, Without these toxins it is unlikely that progress would have been as rapid.

• 전기학회논문지P
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• 제63권2호
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• pp.81-88
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• 2014

In this paper, an implementation of concurrent backoff delay process on a single chip with IEEE 802.15.4 hardware and 8051 processor core that can be used for analyzing the interference on IEEE 802.15.4 channels due to WiFi traffics is studied. The backoff delay process of IEEE 802.15.4 CSMA-CA algorithm is explained. The characteristics of random number generator, timer, and CCA register included in the single chip are described with their control procedure in order to implement the process. A concurrent backoff delay process to evaluate multiple IEEE 802.15.4 channels is proposed, and a method to service the associated tasks at sequentially ordered backoff delay events occurring on the channels is explained. For the implementation of the concurrent backoff delay process on a single chip IEEE 802.15.4 hardware, the elements for the single channel backoff delay process and their control procedure are used to be extended to multiple channels with little modification. The medium access delay on each channel, which is available after execution of the concurrent backoff delay process, is displayed on the LCD of an IEEE 802.15.4 channel analyzer. The experimental results show that we can easily identify the interference on IEEE 802.15.4 channels caused by WiFi traffics in comparison with the way displaying measured channel powers.

• 디지털산업정보학회논문지
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• 제4권1호
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• pp.17-24
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• 2008

This paper describes the measurements that provide a characterization of the code-domain channels of a CDMA base station transmitter. One of the measurements, called code-domain power(CDP), provides the distribution of power in the code domain channels. This measurement can be used to verify that the various channels are at expected power levels and to determine when one code channel is leaking energy into the other code channels. We develop module of CDP measurement in the CDMA system.

• International Journal of Oral Biology
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• 제31권4호
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• pp.135-140
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• 2006

Temperature signaling can be initiated by members of transient receptor potential (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. Since odontoblasts constitute a well-defined layer between the pulp and the mineralized dentin, being first to encounter thermal stimulation from oral cavity, they may be involved in sensory transduction process, in addition to their primary function as formation of dentin. We investigated whether thermo-TRP channels are expressed in a odontoblast cell line, MDPC-23. The expressions of thermo-TRP channels were examined using reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, fluorometric calcium imaging. Analysis of RT-PCR revealed mRNA expression of TRPV1, TRPV2, TRPV4 and TRPM8, but no TRPV3, TRPA1. Immunohistochemical approach failed to detect TRPV1 expression. Whereas the application of 4-phorbol-12,13-didecanoate($10\;{\mu}M$, a TRPV4 agonist), menthol(1 mM, a TRPM8 agonist) and icilin($10\;{\mu}M$, a TRPM8 agonist) produced the enhancement of intracellular calcium concentration, capsaicin($1\;{\mu}M$, a TRPV1 agonist) did not. Our results suggest that subfamily of thermo-TRP channels expressed in odontoblasts may serve as thermal or mechanical transducer in teeth.

• 인터넷정보학회논문지
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• 제15권2호
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• pp.19-31
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• 2014

이 논문에서는 IEEE 802.15.4 노드들이 WiFi 트래픽의 간섭을 피해 새로운 채널에서 동작하기 위해 다수의 IEEE 802.15.4 채널들에 대한 병렬적인 백오프지연과정과 WiFi 트래픽의 주파수 스펙트럼을 고려한 채널탐색방법에 연구되었다. WiFi 트래픽에 의해 점유되는 채널들을 탐색하기 위해, 인접한 채널들에 대한 전력을 동시에 측정하는 방법, 기준보다 큰 채널전력의 지속시간을 확인하는 방법, RSSI 샘플 데이터에 대한 신호처리로 비콘 프레임과 같은 주기성을 찾는 방법에 대해 분석되었다. IEEE 802.11 네트워크와 중첩된 무선채널에서 IEEE 802.15.4 노드들의 CSMA-CA 알고리즘의 동작에 대해서 설명하였다. 하나의 IEEE 802.15.4 장치로 다수의 IEEE 802.15.4 채널에 대해 병렬적으로 백오프지연과정을 수행하는 방법을 그 알고리즘의 설명과 함께 제안하였다. 제안된 방법이 구현된 실험시스템으로 측정된 데이터를 분석할 때, WiFi 트래픽이 발생될 때 이와 연관된 다수의 인접한 IEEE 802.15.4 채널에서 매체접근지연시간이 동시에 증가하는 것으로 관찰되었다. IEEE 802.15.4의 채널에서 다른 트래픽에 의한 간섭을 판단하기 위한 채널평가함수를 정의하였다. WiFi에 의해 간섭을 받는 IEEE 802.15.4 채널들을 탐색하기 위해 인접채널들에 대한 채널평가를 함께 고려하는 채널탐색방법을 제시하였고 실험결과는 WiFi에 의해 간섭이 일어나는 채널들을 올바르게 찾는 특성을 보인다.

• The Korean Journal of Physiology and Pharmacology
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• 제12권2호
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• pp.51-58
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• 2008

Cardiac fibroblasts constitute one of the largest cell populations in the heart, and contribute to structural, biochemical, mechanical and electrical properties of the myocardium. Nonetheless, their cardiac functions, especially electrophysiological properties, have often been disregarded in studies. $Ca^{2+}$-activated $K^+\;(K_{Ca})$ channels can control $Ca^{2+}$ influx as well as a number of $Ca^{2+}$-dependent physiological processes. We, therefore, attempted to identify and characterize $K_{Ca}$ channels in rat Cardiac fibroblasts. First, we showed that the cells cultured from the rat ventricle were cardiac fibroblasts by immunostaining for discoidin domain receptor 2 (DDR-2), a specific fibroblast marker. Secondly, we detected the expression of various $K_{Ca}$ channels by reverse transcription polymerase chain reaction (RT-PCR), and found all three family members of $K_{Ca}$ channels, including large conductance $K_{Ca}$ (BK-${\alpha}1-\;and\;-{\beta}1{\sim}4$subunits), intermediate conductance $K_{Ca}$ (IK), and small conductance $K_{Ca}$ (SK$1{\sim}4$ subunits) channels. Thirdly, we recorded BK, IK, and SK channels by whole cell mode patch clamp technique using their specific blockers. Finally, we performed cell proliferation assay to evaluate the effects of the channels on cell proliferation, and found that the inhibition of IK channel increased the cell proliferation. These results showed the existence of BK, IK, and SK channels in rat ventricular fibroblasts and involvement of IK channel in cell proliferation.