• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.873-882
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• 2008

Eight ileally cannulated pigs (BW $35.9{\pm}0.9kg$) were randomly allotted according to a $4{\times}3$ Latin square design to determine the effects of cellulose, pectin and starch on standardized ileal digestibility (SID) and apparent total tract digestibility (ATTD) of crude protein (CP) and amino acids (AA) as well as on the bacterial AA contribution in feces. The pigs were fed the control diet (20.2% CP, % dry matter (DM)) or one of the three experimental diets in which 25% of the control diet was substituted by cellulose, starch or pectin. Due to this substitution, dietary CP levels were lower in the cellulose (15.5% CP, % DM), pectin (15.4% CP, % DM) and starch diet (15.2% CP, % DM). Following a 15-d adaptation period, feces were collected for 5 d and ileal digesta for a total of 24 h. Starch increased SID of CP, while cellulose and pectin had no significant effect on the digestibility of CP. Overall, starch supplementation resulted in higher (p<0.05) SID values of histidine, isoleucine, threonine, alanine, aspartic acid, cysteine, glycine and serine compared with cellulose, while pectin decreased (p<0.05) SID of valine and proline compared with the starch and control diet. Both cellulose and pectin reduced (p<0.05) the ATTD of CP and AA, while starch decreased (p<0.05) ATTD of phenylalanine, alanine, proline and serine compared with the control. With regard to bacterial AA composition of the fecal mixed bacterial mass (MBM), cellulose supplementation increased (p<0.05) its content of N and almost all AA, except for valine, while pectin caused higher contents of arginine, histidine and proline compared with the control (p<0.05). The bacterial contribution of arginine in feces was higher (p<0.05) in the cellulose treatment, while pectin reduced (p<0.05) the bacterial contribution of leucine, alanine, glutamic acid and proline in feces compared with the control. In conclusion, the effects of cellulose, starch and pectin on SID were rather small. Bacterial activity in the large intestine can only explain the reduced ATTD values for arginine in the cellulose treatment, but not for the other AA in the cellulose and pectin treatments, suggesting higher endogenous losses of these AA in the large intestine.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4369-4376
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• 2015

Background: To investigate in-vitro antagonistic effect of low-dose liquiritigenin on gemcitabine-induced capillary leak syndrome (CLS) in pancreatic adenocarcinoma via inhibiting reactive oxygen species (ROS)-mediated signalling pathways. Materials and Methods: Human pancreatic adenocarcinoma Panc-1 cells and human umbilical vein endothelial cells (HUVECs) were pre-treated using low-dose liquiritigenin for 24 h, then added into gemcitabine and incubated for 48 h. Cell viability, apoptosis rate and ROS levels of Panc-1 cells and HUVECs were respectively detected through methylthiazolyldiphenyl-tetrazoliumbromide (MTT) and flow cytometry. For HUVECs, transendothelial electrical resistance (TEER) and transcellular and paracellular leak were measured using transwell assays, then poly (ADP-ribose) polymerase 1 (PARP-1) and metal matrix proteinase-9 (MMP9) activity were assayed via kits, mRNA expressions of p53 and Rac-1 were determined through quantitative polymerase chain reaction (qPCR); The expressions of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and PARP-1 were measured via western blotting. Results: Low-dose liquiritigenin exerted no effect on gemcitabine-induced changes of cell viability, apoptosis rate and ROS levels in Panc-1 cells, but for HUVECs, liquiritigenin ($3{\mu}M$) could remarkably elevate gemcitabine-induced decrease of cell viability, transepithelial electrical resistance (TEER), pro-MMP9 level and expression of ICAM-1 and VCAM-1 (p<0.01). Meanwhile, it could also significantly decrease gemcitabine-induced increase of transcellular and paracellular leak, ROS level, PARP-1 activity, Act-MMP9 level, mRNA expressions of p53 and Rac-1, expression of PARP-1 and apoptosis rate (p<0.01). Conclusions: Low-dose liquiritigenin exerts an antagonistic effect on gemcitabine-induced leak across HUVECs via inhibiting ROS-mediated signalling pathways, but without affecting gemcitabine-induced Panc-1 cell apoptosis. Therefore, low-dose liquiritigenin might be beneficial to prevent the occurrence of gemcitabine-induced CLS in pancreatic adenocarcinoma.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.651-656
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• 2014

Background: Cervical cancer is the third commonest type of cancer among women in Malaysia. Our aim was to determine the distribution of human papilloma virus (HPV) genotypes in cervical cancer in our multi-ethnic population. Materials and Methods: This was a multicentre study with a total of 280 cases of cervical cancer from 4 referral centres in Malaysia, studied using real-time polymerase chain reaction (qPCR) detection of 12 high risk-HPV genotypes. Results: Overall HPV was detected in 92.5% of cases, in 95.9% of squamous cell carcinomas and 84.3%of adenocarcinomas. The five most prevalent high-risk HPV genotypes were HPV 16 (68.2%), 18 (40%), 58 (10.7%), 33 (10.4%) and 52 (10.4%). Multiple HPV infections were more prevalent (55.7%) than single HPV infections (36.8%). The percentage of HPV positive cases in Chinese, Malays and Indians were 95.5%, 91.9% and 80.0%, respectively. HPV 16 and 18 genotypes were the commonest in all ethnic groups. We found that the percentage of HPV 16 infection was significantly higher in Chinese (75.9%) compared to Malays (63.7%) and Indians (52.0%) (p<0.05), while HPV 18 was significantly higher in Malays (52.6%) compared to Chinese (25.0%) and Indians (28%) (p<0.05). Meanwhile, HPV 33 (17.9%) and 52 (15.2%) were also more commonly detected in the Chinese (p<0.05). Conclusions: This study showed that the distribution of HPV genotype in Malaysia is similar to other Asian countries. Importantly, we found that different ethnic groups in Malaysia have different HPV genotype infection rates, which is a point to consider during the implementation of HPV vaccination.

• Ocean and Polar Research
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• 제34권1호
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• pp.93-99
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• 2012

The effect of water temperature and body weight on oxygen consumption by the fasted olive flounder Paralichthys olivaceus was investigated in order to assess the metabolic rate of this species under different conditions. The oxygen consumption rate (OCR) was measured at three different water temperatures (15, 20 and $25^{\circ}C$) and two different body weights [$9.1{\pm}1.2$ g (mean${\pm}$SD) for the juvenile group and $266.4{\pm}29.3$ g for the immature group] at an interval of 5 minutes for 24 hours using a closed flow-through respirometer. For each treatment condition, three replicates were set up and 135 fish in the juvenile group and 18 fish in the immature group were used. The OCRs exhibited a linear increase described by OCR=-82.06+28.30T ($r^2$=0.96, p<0.001) in the juvenile group and OCR=-52.52+14.73T ($r^2$=0.97, p<0.001) in the immature group. The OCRs decreased with increasing body weights at a given water temperature (p<0.001). The metabolic rate was related to the body weight of the fish as a power function with a weight exponent of between 0.77 and 0.82. $Q_{10}$ values ranged 1.67~2.28 when the temperature was between 15 and $20^{\circ}C$, 1.57~1.93 when the temperature was between 20 and $250^{\circ}C$, and 1.79~1.89 when the temperature was between 15 and $250^{\circ}C$. The energy expenditure by respiration increased with increasing water temperature and decreasing body weight (p<0.001). The mean energy loss rates at 15, 20 and $25^{\circ}C$ were 115.9, 149.8 and 208.2 kJ $kg^{-1}d^{-1}$ in the juvenile groups and 53.8, 81.2 and 101.9 kJ $kg^{-1}d^{-1}$ in the immature groups.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1197-1205
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• 2006

This experiment was conducted to compare the effects of feeding DL-2-hydroxy-4-(methylthio)butanoic acid (HMTBA) and DL-methionine (DLM) supplemented corn-soybean-cottonseed meal diets on growth performance, carcass composition, and muscle color of broilers. The trial was designed as a $2{\times}3{\times}2$ factorial experiment, including two methionine (Met) sources (HMTBA and DLM), three equimolar graded levels of Met supplementation (i.e., 0.08, 0.16, and 0.24% in the starter diet and 0.07, 0.14, and 0.21% in the grower and finisher diets, respectively), and two sexes (male and female). Additionally, one basal diet for each sex was formulated to be limiting in Met to test the dosage response of increasing supplemental Met levels. Four hundred and twenty 10-d-old broilers were randomly allotted to 14 treatments (seven each for males and females), with five replicate pens per treatment and six chicks per pen. There was no difference (p>0.05) between the two Met sources in growth performance and muscle deposition of broilers throughout the whole experimental period (d 10 to 49). With the increasing Met supplementation levels, average daily gain was increased (quadratic; p<0.01) during the starter, grower, and overall phases, average daily feed intake was increased (quadratic; p<0.01) during the starter phase, and feed:gain ratio was decreased (quadratic; p<0.05) during the grower and overall phases. At the end of finisher phase, Met supplementation increased breast muscle content (quadratic; p<0.01) and thigh muscle content (linear; p<0.05), and decreased abdominal fat content (quadratic; p<0.02). Compared to the broiler fed DLM, broilers fed HMTBA had superior breast and thigh muscle coloration (p<0.01). Male broilers had higher weight gain and feed intake and better feed conversion than female broilers (p<0.01). The fat content of thigh muscle in female broilers was higher than that of male broilers (p<0.03). The best fit comparison of HMTBA vs. DLM was determined by Schwarz Bayesian Criteria index, which indicated that the average relative bioefficacy of HMTBA vs. DLM was 120% with 95% confidence limit 67 to 172%. These results indicated that Met supplementation improved growth performance and carcass quality of broilers fed corn-soybean-cottonseed meal diets irrespective of Met sources. Compared to DLM, HMTBA has the same molar bioefficacy on improving the growth performance and carcass quality of broilers; however, HMTBA fed birds had superior meat color to DLM fed birds.

• Asian-Australasian Journal of Animal Sciences
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• 제29권12호
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• pp.1768-1773
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• 2016

This experiment was conducted to determine the effect of variety and planting year on the nutritive values of corn fed to growing pigs. Four corn varieties examined in this experiment were planted in the same village located in Longhua County, Heibei Province, China, in 2012, 2013, and 2014, respectively. During each year, corn was hand-harvested in early October and sun dried to about 14% moisture content. Three batches of twenty-four barrows ($33.27{\pm}4.30$, $31.88{\pm}2.93$, $34.21{\pm}3.81kg$ body wight [BW] in 2012, 2013, and 2014, respectively) were used and allotted to a complete block design with 4 diets and 6 replicate pigs per diet. Pigs were individually placed in metabolic crates. The four experimental diets were formulated by mixing each variety of corn and vitamins and minerals, respectively. A five-day collection period followed a seven-day diet acclimation period. The results indicated that variety of corn significantly influenced the available energy content (digestible energy [DE] on dry matter basis, p<0.05; metabolizable energy (ME) on dry matter basis, p<0.05, respectively), and the apparent total tract digestibility (ATTD) of organic matter (p<0.01), dry matter (p<0.05), gross energy (p<0.05), neutral detergent fiber (p<0.01), acid detergent fiber and ether extract (p<0.05). The planting year also significantly influenced the available energy contents (DE on dry matter basis, p<0.05; ME on dry matter basis, p<0.01, respectively) and the ATTD of neutral detergent fiber (p<0.01), acid detergent fiber (p<0.01), crude protein (p<0.01), and ether extract (p<0.01). No interaction was observed between the variety and planting year in DE and ME contents in corn. In conclusion, the variety and planting year significantly influenced the available energy and nutrient digestibility of corn fed to growing pigs.

• 한국의학물리학회지:의학물리
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• 제13권2호
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• pp.85-89
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• 2002

목적 : 사람에 비하여 크기가 현저히 작은 동물의 자기공명영상 촬영을 위하여 상용으로 인체에 적용하는 Head 코일보다 원통 반지름이 작은 Low-Pass Type Birdcage 코일을 제작함으로써 보다 큰 신호대잡음비 (Signal-to-Noise Ratio. SNR)를 얻고자 하였다. 방법 : 자기공명영상을 얻기 위해 Spin Echo 펄스시퀀스와 Fast Spin Echo 펄스시퀀스를 사용하였다. T1 강조영상을 얻기 위한 Spin Echo 펄스시퀀스의 매개변수는 TR/TE=300/17 ㎳, Matrix=256$\times$256, Field Of View (FOV)=150 mm, Slice Thickness=2 mm 이었다. T2 강조영상을 얻기 위한 Fast Spin Echo 펄스시퀀스의 매개변수는 TR/TE=3000/96 ㎳, Matrix=256$\times$256, Field Of View (FOV)=150 mm, Slice Thickness=2 mm 이었다. 원통의 지름이 13 cm인 Birdcage 코일은 12개의 elements로 구성되어 있으며 길이는 22 cm로 제작되었다. 결과 : 코일 원통의 반지름의 크기에 따른 SNR을 비교하기 위하여 인체용 Knee 코일과 동물용으로 제작된 코일을 이용하여 각각의 팬톰 영상을 획득하였다. 팬톰 영상으로부터 측정된 SNR의 값을 통해 반지름이 작은 동물용 코일의 SNR이 더 크다는 걸 확인할 수 있었다. 토의 및 결론 : 본 연구를 통하여 같은 형태의 Birdcage 코일일 경우 원통의 반지름에 따라 SNR이 다르며, 특히 반지름이 작을 때 SNR이 더 크다는 것을 알 수 있었다. 따라서 코일의 크기에 비해 촬영하고자 하는 대상물의 부피가 작은 경우 대상물의 부피에 맞추어 코일을 제작하면 SNR이 보다 뛰어난 영상을 얻을 수 있을 것으로 사료된다.

• 물과 미래
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• 제5권2호
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• pp.17-29
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• 1972

The persent study aims at finding out a means of prevention cool spell damages on the hilly areas. The irrigation plots of 24 hour stored water warm water way and warm water plots, cool water way are respectively established to find out water temperature and influnce on the growing rice plants. The results obtained are summed up as follows. 1. Warm water areas consisted of $5 m^2 Q=0.93 1{\ell}/sec$, V=31 cm/sec, S=1/1, 000, L=81.6m, B=5cm, h=6cm, t=4min 33sec, drops=9 areas, are constructed to help the water temperature of $14.5^{\circ}C$ rise to that of $21.6^{\circ}C$. This indicates lower temperature than $23^{\circ}C$ of critical water temperature in irrigation facilities by $1.45^{\circ}C$ and than $26.2^{\circ}C$ of balanced water temperature of Seoul arears by $4.6^{\circ}C$. But this does not give much influance on rice plant cultivation. 2. The rising of water temperature is influened according to the temperature, solar radiation but the water temperature changes according to the heat absorption of organized materials, weather and terraces. The difference of water temperature could be found in the first growing stage. 3. Through the warm water way of water rises to the temperature of $21.6^{\circ}C$ which also rises to the temperature of around $30^{\circ}C$ in the paddy field of submerged irrigation. The rice plants are comparatively free from prolonged cool damage, reproduction abstructive damage. 4. The water temperature in rice field in proportion to temperature influence of weather condition but the water temperature approaches to that of weather in the days of later growing stage and water temperature become lower than the air temperature in the fruit stage. 5. The water in the submreged field is $10^{\circ}C$ warmer than in the warm water way during the first growing stage period but the water temperature in the warm water way is warmer in the later growing stage period. The cool water of $14.5^{\circ}C$ is warmed to $30.1^{\circ}C$ and rice plants cultivation is free from other damages. 6. The 12% increased production or 570.98kg/10a is made cool water plot by rising the temperature of water from $14.5^{\circ}C$ to $21.6^{\circ}C$ making the water run through warm water way. 7. The damage inflicted by the cool water irrigation during the first growing stage period is the obstruction of peak tillering stage and the obstruction of heading the later growing stage period and the obstruction of fruiting and number of panides per fill.

• 한국작물학회지
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• 제46권5호
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• pp.387-394
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• 2001

Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

• Childhood Kidney Diseases
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• 제12권1호
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• pp.88-92
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• 2008

막성 신병증은 성인에 비해 소아에서는 드문 신병증으로 감염, 자가면역, 종양에 의한 이차성 신병증이 알려져 있다. 그 중에서도 엡스타인-바 바이러스 감염과의 관련은 잘 알려지지 않아, 최근 1개 보고에서 면역기능이 저하된 2례를 소개한 바 있을 뿐이다. 이에 저자들은 면역기능이 정상으로 보이는 소아에서 엡스타인-바 바이러스 감염과 관련한 막성 신병증 1례를 경험하였기에 보고하는 바이다.