• Title/Summary/Keyword: 3P

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MiR-141-3p regulates myogenic differentiation in C2C12 myoblasts via CFL2-YAP-mediated mechanotransduction

  • Nguyen, Mai Thi;Lee, Wan
    • BMB Reports
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    • v.55 no.2
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    • pp.104-109
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    • 2022
  • Skeletal myogenesis is essential to keep muscle mass and integrity, and impaired myogenesis is closely related to the etiology of muscle wasting. Recently, miR-141-3p has been shown to be induced under various conditions associated with muscle wasting, such as aging, oxidative stress, and mitochondrial dysfunction. However, the functional significance and mechanism of miR-141-3p in myogenic differentiation have not been explored to date. In this study, we investigated the roles of miR-141-3p on CFL2 expression, proliferation, and myogenic differentiation in C2C12 myoblasts. MiR-141-3p appeared to target the 3'UTR of CFL2 directly and suppressed the expression of CFL2, an essential factor for actin filament (F-actin) dynamics. Transfection of miR-141-3p mimic in myoblasts increased F-actin formation and augmented nuclear Yes-associated protein (YAP), a key component of mechanotransduction. Furthermore, miR-141-3p mimic increased myoblast proliferation and promoted cell cycle progression throughout the S and G2/M phases. Consequently, miR-141-3p mimic led to significant suppressions of myogenic factors expression, such as MyoD, MyoG, and MyHC, and hindered the myogenic differentiation of myoblasts. Thus, this study reveals the crucial role of miR-141-3p in myogenic differentiation via CFL2-YAP-mediated mechanotransduction and provides implications of miRNA-mediated myogenic regulation in skeletal muscle homeostasis.

Rotavirus P and G Genotypes Circulating in Kyungsangnamdo, Korea, during 2000~2001 (2000~2001년 경상남도에서 유행한 로타바이러스 유전자형)

  • So, Kyeung Jin;Lee, Mi Hyun;Ma, Sang Hyeok;Kim, Byung Chyeol;Yang, Jai Myung
    • Pediatric Infection and Vaccine
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    • v.11 no.1
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    • pp.59-72
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    • 2004
  • Purpose : Rotaviruses are the major cause of gastroenteritis in infants and young children worldwide. It is important to get the epidemiologic data of rotavirus genotype for the application of rotavirus vaccine. So we tried to investigate the distribution of rotavirus genotypes with RT-PCR. Methods : A total of 120 rotavirus latex agglutinin test positive stool samples were collected continually from 120 children from Sep. 2000 to Apr. 2001. Rotavirus P(VP4), G (VP7) genotypes were determined by RT-PCR. Results : The genotype was identified in 116 stool samples of total 120 samples(96%). The incidence of G genotype was as follow; G1 17(14.2%), G2 74(61.7%), G4 1(0.8%), G9 1(0.8%). There were four cases of multiple genotypes; G1/G2, G1/G4, G1/G9, G8/G9 and genotype of G3, G8 were not found. Twenty three(19.2%) samples were nontypeable. The incidence of P was as follow; P[4] 77(64.2%), P[6] 22(18.3%), P4/P6 12(10%), P[4]/P[8] 1(0.8%) p[8] 1(0.8%). Seven(5.9%) samples were nontypeable. Conclusion : Various combinations of G and P genotypes were observed. Most rotavirus strains were P[4]G2 62(51.74%), followed by P[6]G2 7(5.8%), and P[6]G1 7(5.8%), P[4/P[6] G1 4(3%), P[4]/P[6]G2 4(3%), P[4]G1 3(2.5%), P[8]G2 1(0.8%), P[4]G4 1(0.8%) in Kyoungsangnamdo, Korea during 2000~2001.

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A P2P System Design Using UML Based On 3-Tier Environment (UML을 이용한 3-Tier 환경에서의 p2p 시스템 설계)

  • 이승아;권영직
    • Proceedings of the Korea Society for Industrial Systems Conference
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    • 2002.11a
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    • pp.331-335
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    • 2002
  • 인터넷의 발전은 사용자들의 정보에 대한 요구를 급속히 증가시키고 있으며, 고급 정보 획득을 위한 사용료에 대한 부담도 함께 증가하고 있다. 따라서 사용자 각자가 보유한 정보들을 다른 사용자들에게 오픈 시켜 공유할 수 있도록 하는 시스템의 개발에 대한 요구가 발생하게 되었다. 이러한 요구에 맞추어 본 논문에서는 네티즌들이 서로의 컴퓨터에 접속하여 상대방이 가진 정보들을 검색하고 공유할 수 있는 p2p(peer to peer) 시스템인 $\ulcorner$p2p-3$\lrcorner$을 제안하게 되었다. $\ulcorner$p2p-3$\lrcorner$시스템은 객체지향설계 기법인 UML을 이용하여 개발함으로써 시스템 구현의 효율성을 추구하였다. 또한 일반적인 p2p 시스템의 문제점으로 대두된 보안문제를 해결하기 위하여 3-tier 분산시스템을 이용하였다.

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Effect of Propolis Addition on the Shelf-Life and Staling of White Bread (Propolis의 첨가가 식빵의 저장수명과 노화에 미치는 영향)

  • Kim, Chong-Tai;Lee, Soo-Jeong;Hwang, Jae-Kwan;Kim, Chul-Jin;Ahn, Byung-Hak
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.982-986
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    • 1997
  • Propolis extract was added to white bread(P1-1.48%, P2-0.74%, P3-0.37%), prepared in the straight-dough method, and its effects on shelf-life, antimicrobial activity, staling and sensory evaluation of white bread were investigated. In all P1, P2 samples, added propolis inhibited the growth of fungi, and the more propolis extract was added, the higher degree of inhibition of fungal growth was observed. The staling rates of white breads with P1, P2 and P3 were retarded by 22.5%, 19.2% and 6.4% respectively compared to that of control, and the Avrami exponent was similar in all samples. As a result of sensory evaluation, flavor, off-flavor, texture and overall acceptability of P2 and P3 were not significantly different from that of control.

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he Effects of Supplemental Levels of Bamboo Vinegar on Growth Performance, Serum Profile and Meat Quality in Fattening Hanwoo Cow (죽초액의 첨가수준이 비육 한우암소의 육생산성, 혈액성상 및 육질에 미치는 영향)

  • Kook, K.;Kim, K. H.
    • Journal of Animal Science and Technology
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    • v.45 no.1
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    • pp.57-68
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    • 2003
  • Effects of supplemental levels of Bomboo vinegar(BV) on growth performance, serum profile and meat quality in 15 Korean native Hanwoo cows was investigated. Concentrate diet was supplemented with Bamboo vinegar with 3% and 6% of the diet. Daily weight gain was increased slightly at 3% BV, but it was decreased in 6% BV. Feed intake was decreased(P<0.05) at 6% BV. Glucose concentration of serum profile was decreased(P<0.05) at 3% and 6% BV. Total protein and cholesterol concentrations were increased(P<0.05) at 3% and 6% BV. BUN concentration was increased(P<0.05) at 3% BV. In carcass characteristics the longissimus muscles of all BV treatments showed no significant(P<0.05) effects, but back fat thickness was decreased significantly(P<0.05) in 6% BV. Marbling score was increased significantly(P<0.05) at 3% BV, thus improving the meat quality. Cut meat production was not difference by treatment. Crude fat content of proximate chemical composition in longissimus muscle was increased(P<0.05) in 3% BV. Shear force and cholesterol contents were decreased(P<0.05) in 3% and 6% BV. The 16:0 of fatty acid composition in longissimus was decreased(P<0.05) whereas 18:1 was increased(P<0.05) at 3% and 6% BV. The composition of saturated fatty acids(SFA) was decreased(P< 0.05), whereas unsaturated fatty acids(USFA) was increased(P<0.05) in 3% BV. Odor and appearance of sensory evaluation were not difference by treatment. Taste was improved significantly (P<0.05) in 3% and 6% BV with the peculiar and savory taste of Hanwoo being more emphasized. The results of this experiment indicated that 3% BV improved the marbling score and crude fat content, decreased the shear force and cholesterol contents, increased the USFA composition, and improved the taste of sensory evaluation in Korean native Hanwoo cows.

New Approach on the Extinction of Spreading at the Phoenix Ridge, Antarctica (남극 피닉스 해령 확장작용 소멸시기에 대한 새로운 고찰)

  • Choe Won Hie;Lee Jong Ik;Lee Mi Jung;Hur Soon Do;Jin Yaung Keun
    • The Journal of the Petrological Society of Korea
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    • v.14 no.1
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    • pp.73-81
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    • 2005
  • K-Ar ages have been determined for the submarine basalts dredged from the P2 and P3 segments of the Phoenix Ridge, Drake Passage, Antarctica, for better understanding on the extinction of seafloor spreading. At the P3 segment, the K-Ar ages of the rifted ridge basalts are 3.5-6.4 Ma, and those for the axial seamount basalts are 1.5-3.1 Ma. The K-Ar ages for the basalts at the rifted ridge and axial central high in the P2 segment are 2.1 and 1.4-1.9 Ma, respectively. We suggest that the extinction of seafloor spreading at the P3 and P2 segments occurred at 3.3 and 2.0 Ma, respectively, on the basis of ridge structure and formation time of basalts. This result favors a stepwise extinction model rather than a simultaneous one on the extinction of the Phoenix Ridge.

A Mutual P3P Methodology for Privacy Preserving Context-Aware Systems Development (프라이버시 보호 상황인식 시스템 개발을 위한 쌍방향 P3P 방법론)

  • Kwon, Oh-Byung
    • Asia pacific journal of information systems
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    • v.18 no.1
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    • pp.145-162
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    • 2008
  • One of the big concerns in e-society is privacy issue. In special, in developing robust ubiquitous smart space and corresponding services, user profile and preference are collected by the service providers. Privacy issue would be more critical in context-aware services simply because most of the context data themselves are private information: user's current location, current schedule, friends nearby and even her/his health data. To realize the potential of ubiquitous smart space, the systems embedded in the space should corporate personal privacy preferences. When the users invoke a set of services, they are asked to allow the service providers or smart space to make use of personal information which is related to privacy concerns. For this reason, the users unhappily provide the personal information or even deny to get served. On the other side, service provider needs personal information as rich as possible with minimal personal information to discern royal and trustworthy customers and those who are not. It would be desirable to enlarge the allowable personal information complying with the service provider's request, whereas minimizing service provider's requiring personal information which is not allowed to be submitted and user's submitting information which is of no value to the service provider. In special, if any personal information required by the service provider is not allowed, service will not be provided to the user. P3P (Platform for Privacy Preferences) has been regarded as one of the promising alternatives to preserve the personal information in the course of electronic transactions. However, P3P mainly focuses on preserving the buyers' personal information. From time to time, the service provider's business data should be protected from the unintended usage from the buyers. Moreover, even though the user's privacy preference could depend on the context happened to the user, legacy P3P does not handle the contextual change of privacy preferences. Hence, the purpose of this paper is to propose a mutual P3P-based negotiation mechanism. To do so, service provider's privacy concern is considered as well as the users'. User's privacy policy on the service provider's information also should be informed to the service providers before the service begins. Second, privacy policy is contextually designed according to the user's current context because the nomadic user's privacy concern structure may be altered contextually. Hence, the methodology includes mutual privacy policy and personalization. Overall framework of the mechanism and new code of ethics is described in section 2. Pervasive platform for mutual P3P considers user type and context field, which involves current activity, location, social context, objects nearby and physical environments. Our mutual P3P includes the privacy preference not only for the buyers but also the sellers, that is, service providers. Negotiation methodology for mutual P3P is proposed in section 3. Based on the fact that privacy concern occurs when there are needs for information access and at the same time those for information hiding. Our mechanism was implemented based on an actual shopping mall to increase the feasibility of the idea proposed in this paper. A shopping service is assumed as a context-aware service, and data groups for the service are enumerated. The privacy policy for each data group is represented as APPEL format. To examine the performance of the example service, in section 4, simulation approach is adopted in this paper. For the simulation, five data elements are considered: $\cdot$ UserID $\cdot$ User preference $\cdot$ Phone number $\cdot$ Home address $\cdot$ Product information $\cdot$ Service profile. For the negotiation, reputation is selected as a strategic value. Then the following cases are compared: $\cdot$ Legacy P3P is considered $\cdot$ Mutual P3P is considered without strategic value $\cdot$ Mutual P3P is considered with strategic value. The simulation results show that mutual P3P outperforms legacy P3P. Moreover, we could conclude that when mutual P3P is considered with strategic value, performance was better than that of mutual P3P is considered without strategic value in terms of service safety.

Conducting Polymer Material Characterization Using High Frequency Planar Transmission Line Measurement

  • Cho, Young-Seek;Franklin, Rhonda R.
    • Transactions on Electrical and Electronic Materials
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    • v.13 no.5
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    • pp.237-240
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    • 2012
  • A conducting polymer, poly 3-hexylthiophene (P3HT) is characterized with the metal-insulator-semiconductor (MIS) measurement method and the high frequency planar circuit method. From the MIS measurement method, the relative dielectric constant of the P3HT film is estimated to be 4.4. For the high frequency planar circuit method, a coplanar waveguide is fabricated on the P3HT film. When applying +20 V to the CPW on P3HT film, the P3HT film is in accumulation mode and becomes lossy. The CPW on P3HT film is 1.5 dB lossier than the CPW on $SiO_2$ film without P3HT film at 50 GHz.

Low Spin-Casting Solution Temperatures Enhance the Molecular Ordering in Polythiophene Films

  • Lee, Wi Hyoung;Lee, Hwa Sung;Park, Yeong Don
    • Bulletin of the Korean Chemical Society
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    • v.35 no.5
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    • pp.1491-1494
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    • 2014
  • High-crystallinity poly(3-hexylthiophene) (P3HT) thin films were prepared by aging the precursor solutions, prepared using a good solvent, chloroform, at low temperatures prior to spin-casting. Lower solution temperatures significantly improved the molecular ordering in the spin-cast P3HT films and, therefore, the electrical properties of field-effect transistors prepared using these films. Solution cooling enhanced the electrical properties by shifting the P3HT configuration equilibrium away from random coils and toward more ordered aggregates. At room temperature, the P3HT molecules were completely solvated in chloroform and adopted a random coil conformation. Upon cooling, however, the chloroform poorly solvated the P3HT molecules, favoring the formation of ordered P3HT aggregates, which then yielded more highly crystalline molecular ordering in the P3HT thin films produced from the solution.