• Horticultural Science & Technology
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• v.34 no.6
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• pp.924-939
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• 2016

The great economic losses caused by Cucumber mosaic virus (CMV) infection of peppers has led to the development of genetically modified (GM) CMV-resistant peppers. We developed virus-resistant pepper plants using Agrobacterium tumefaciens -mediated transformation. The expressed recombinant protein was purified using nickel-nitrilotriacetic acid resin and immunoaffinity chromatography, and purity was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoblot analysis revealed the purified CMV coat protein (CMV-CP) had a molecular mass of 25 kDa. After in-gel digestion and desalting, the internal peptide fragments of CMV-CP were sequenced by matrix-assisted laser desorption/ionization-time of flight. Most GM pepper and Escherichia coli BL21 internal peptides had identical peptide sequences and contained 137 of 183 whole peptides in CMV-CP. A quantitative enzyme-linked immunosorbent assay was performed to detect CMV-resistant GM peppers. We also provide basic information about the expressed protein in GM peppers for further safety assessment. The contents of soluble protein and CMV-CP were measured in GM and control peppers cultivated in three different areas of Korea. Statistical significance in terms of cultivation areas, harvest times, generations, and plant tissue origin were determined based on a P value of 0.05. The highest amount of CMV-CP was detected at the seedling stage from plant grown in each region. T3 and T5 showed significantly different levels of CMV-CP from T4 in leaves in the whorl stage. No statistical differences were observed among GM peppers at different stages of maturity in any cultivation area. The results from this study contribute to the safety evaluation of newly designed CMV-resistant GM peppers and provide a standard against which to compare other virus-resistant GM peppers.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.2 no.1
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• pp.77-79
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• 2002

The urea cycle, consisting of a series of six enzymatic reactions, plays key roles to prevent the accumulation of toxic nitrogenous compound and synthesize arginine de novo. Five well characterized diseases have been described, resulting from an enzymatic defect in the biosynthesis of one of the normally expressed enzyme. This presentation will focus on two representative diseases; ornithine transcarbamylase(OTC) deficiency and citrullinemia(argininosuccinate synthetase deficiency). OTC deficiency is one of the most common inborn error of urea cycle, which is inherited in X-linked manner. We identified 17 different mutations in 20 unrelated Korean patients with OTC deficiency; L9X, R26P, R26X, T44I, R92X, G100R, R141Q, G195R, M205T, H214Y, D249G, R277W, F281S, 853 del C, R320X, V323M and 10 bp del at nt. 796-805. These mutations occur at well conserved nucleotide sequences across species or CpG hot spot. The L9X and R26X lead to the disruption of leader sequences, required for directing mitochondrial localization of the OTC precursor. Their phenotypes are severe, and neonatal onset. The G100R, R277W and V323M mutations were uniquely identified in patients with late onset OTC deficiency. The other genotypes are associated with neonatal onset. Out of 20 patients with OTC deficiency, only 6 patients are alive; two were liver transplanted, and normal in growth and development at 2, 4 years after transplantation respectively. Citrullinemia is an autosomal recessive disease, caused by the mutations in the argininosuccinate synthetase(ASS) gene. We identified in 3 major mutations in 11 unrelated Korean patients with citrullinemia; G324S, $IVS6^{-2}$ A to G, and 67 bp ins at nt 1125-1126. Among these, the 67 base pair insertion mutation is novel. The allele frequency of each mutation is; G324S(45%), IVS6-2 A to G(32%), and 67 base pair insertion(14%). All patients are diagnosed at neonatal or infantile age. Interestingly, two patients presented with stroke like episode. Out of 11 patients, 5 patients died. Among 6 patients alive, one patient was successfully liver transplanted.

• Journal of Korea Spatial Information System Society
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• v.3 no.1 s.5
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• pp.49-62
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• 2001

Recently, component-based software has become main trends in designing and developing computer software products. This component-based software has advantage of the interoperability on distributed computing environment and the reusability of pre-developed components. Also, GIS is designed and implemented with this component-based methodology, called Open GIS Component. OGC(OpenGIS Consortium) have announced various implementation and design specification and topic in GIS. In GIS, Spatial analysis functions like network analysis, TIN analysis are very important function and basically, estimate system functionality and performance using this analysis methods. The simple feature geometry specification is announced by OGC to increase the full interoperability of various spatial data. This specification includes just geometry spatial data model. However, in GIS which manages spatial data, not only geometric data but also topological data and various analysis functions have been used. The performance of GIS depends on how this geometric and topological data is managed well and how various spatial analyses are executed efficiently. So it requires integrated spatial data model between geometry and topology and extended data model of topology for spatial analysis, in case network analysis and TIN analysis in open GIS component. In this paper, we design analysis component like network analysis component and TIN analysis component. To manage topological information for spatial analysis in open GIS component, we design extended data model of simple feature geometry for spatial analysis. In addition to, we design the overall system architecture of open GIS component contained this topology model for spatial analysis.

• Korean Journal of Ecology and Environment
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• v.33 no.1 s.89
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• pp.38-50
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• 2000

Seasonal changes of the phytoplankton and the periphyton community were investigated from August 1998 to April 1999 at five stations at the Suer stream in Kwangyang. A total of 112 species of phytoplankton were identified. Of those, the diatoms were present at all stations but the green algae, the bluegreen algae, and the dioflagellates were present at Station 4 and Station 5 more frequently than the other stations. The phytoplankton standing crops varied from 10,100 cells/1 at Station 4 in April 1999 to 1,489,100 cells/1 at Station 4 in October 1998. The seasonal variation patterns of phytoplankton standing crops were different among stations as well as the pattern of presence. The dominant species were as follows: Achnanthes minutissima, Aulacoseira distans v. alpigena, Cocconeis placentula v. lineata, Cymbella minuta, C. silesiaca, Fragilaria arcus v. recta, Peridinium cinctum, Rhizosofenia longiseta, Synedra rumpens and filamentous algae. Of those, Achnanthes minutissima, Rhizosolenia longiseta, Synedra rumpens and filamentous algae showed the highest rate of occupation in the phytoplankton standing crops during the investigated periods. A total of 99 species of periphyton were identified. Among those, the diatoms of the periphyton community were observed frequently rather than those of the phytoplankton community. The ecological indicator values showed ${\bate}$-mesosaprobous in saprobity and was close to eutraphentic in trophic state. There were no considerable differences between the ecological indicator values by planktonic diatoms and periphytic diatoms.

• Korean Journal of Food Science and Technology
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• v.41 no.2
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• pp.186-190
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• 2009

Four different mixing ratios of Rose rugosa Thun and Vitis amurensis (Moru) were prepared. These included Rose rugosa Thun two vs. Moru one (Moru 33), Rose rugosa Thun one vs. Moru two (Moru 67), Rose rugosa Thun one vs. Moru five (Moru 83), and Moru (Moru 100). Their physiochemical changes were investigated during 28 days of fermentation followed by aging. The final brix, pH, and total titratable acidity values of the four experiments were in the following ranges: 6.0-8.2$^{\circ}$Bx, 3.87-4.03, and 0.94-1.18%, respectively. Final ethanol contents were 6.5% in Moru 33, 11.8% in Moru 67, 11.1% in Moru 83, and 11.4% in Moru 100. As the amount of Rose rugosa Thun increased, anthocyanin content, color intensity, organic acid, and free amino acid concentrations were reduced. These findings demonstrate that the supplementation of Rose rugosa Thun to Moru prior to alcohol fermentation may help change the acidity, colour, and taste in the final product.

• Korean Journal of Food Science and Technology
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• v.46 no.2
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• pp.231-236
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• 2014

The present study was undertaken to evaluate the anti-hyperlipidemic activity of Albizzia julibrissin and Cassia tora L. extract mixtures in high fat diet-induced hyperlipidemic rats. In high-fat diet fed hyperlipidemic rats, oral treatment with Albizzia julibrissin and Cassia tora L. extracts resulted in decreased levels of total serum cholesterol and triglycerides, epididymal fat pad (EFP) weights, and activities of hepatic glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme, while the levels of feces production and high-density lipoprotein (HDL)-cholesterol were increased, compared with untreated control. Therefore, these results suggest that Albizzia julibrissin and Cassia tora L. extract mixtures can ameliorate hyperlipidemia.

• Korean Journal of Head & Neck Oncology
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• v.13 no.2
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• pp.161-168
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• 1997

Background: Cancers of the maxillary sinuses are not common and are the most difficult head and neck malignancies in which to make an early diagnosis. Objectives: This reports was conducted to evaluated the efficacy of combination therapy and the relationship between the treatment modalities and their outcome of maxillary sinus cancers. Materials and Methods: We retrospectively analyzed a clinical datas of 46 patients who were treated at the department of Otolaryngology-Head and Neck Surgery. The Catholic University of Korea over 10 years between 1987 and 1996. Results: According to AJCC TNM system, 35 patients presented with $T_4$, 10 with $T_3$, one with T1. Two patients were treated with radiotherapy alone, 4 patients with chemotherapy alone, 17 patients with radiotherapy and chemotherapy, 23 patients with combination of surgery, radiotherapy and chemotherapy. The overall 5 years survival rate for combination therapy group were 57%, but 23 patients treated with the other treatment modalities all died within 2 years except two cases with chemotherapy and radiotherapy or radiotherapy alone. There was a statistical trend for better survival and local control in those patients treated with combination therapy than others(p<0.05). Conclusion: The results of this study suggest that it may be possible to acheive better results with aggressive combination treatment including surgery in advanced cases and to avoid orbital excentration in patients with orbital invasion.

• Korean Journal of Head & Neck Oncology
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• v.30 no.2
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• pp.53-61
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• 2014

Background and Objectives : Anaplastic thyroid carcinoma(ATC) is a rare but highly aggressive thyroid malignancy that is associated with an extremely poor survival despite the best multidisciplinary care. BRAF(V600E) mutation is detected in about a quarter of ATC, but unlike its high treatment response to selective BRAF inhibitor (PLX4032) in metastatic melanoma, the treatment response of ATC is reported to be low. The purpose of this study is to investigate the innate resistance mechanism responsible for this low treatment response to BRAF inhibitor and its effect on epithelial-mesenchymal transition(EMT). Materials and Methods : Two ATP cell lines, 8505C and FRO were selected and treated with PLX4032 and its drug sensitivity and effects on cell migration and EMT were examined and compared. Further investigation on the changes in signals responsible for the different treatment response to PLX4032 was carried out and the same experiment was performed on both orthotopic and ectopic xenograft mouse models. Results : FRO cell line was more sensitive to PLX4032 treatment compared to 8505C cell line. The resistance to BRAF inhibition in 8505C was due to increased expression of EGFR. Effective inhibition of both EGFR and p-AKT was achieved after dual treatment with BRAF inhibitor(PLX4032) and EGFR inhibitor(Erlotinib). Similar results were confirmed on in vivo study. Conclusion : EGFR-mediated reactivation of the PI3K/AKT pathway and MAPK pathway contributes to the relative insensitivity of BRAF(V600E) mutant ATC cells to PLX4032. Dual inhibition of BRAF and EGFR leads to sustained treatment response including cell invasiveness.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.1205-1208
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• 2010

본 연구는 초등학교 5, 6학년 학생의 인터넷 중독과 학교생활 적응과의 관계를 파악하여 인터넷 사용에 대한 교육적 지도방안과 인터넷 중독 예방프로그램 개발의 기초 자료를 제공하고자 시도되었다. 자료는 2009년 7월 1일에서 7월 24일까지 제주특별자치도에 위치한 초등학교 6개교(동지역 4개교, 읍지역 2개교)를 무작위 추출하여 5, 6학년 학생 총 1195명을 대상으로 조사하였다. 자료 분석은 SPSSWIN(12.0 한글판) 프로그램을 이용하여 카이제곱검정, t-검정, 일원배치 분산분석, 상관분석, 다중회귀분석의 통계적 방법을 이용하였다. 본 연구의 주요 결과는 첫째, 대상자의 인터넷 중독 정도는 고위험군 55명(5.5%), 잠재적위험군 49명 (4.9%), 정상군 900명(89.6%)으로 나타났으며, 둘째, 대상자의 일반적 특성에 따른 인터넷 중독 정도를 분석한 결과 성별, 형제자매 유무, 학교성적에 따라 유의한 차이가 있었다. 셋째, 대상자의 컴퓨터 사용 특성에 따른 인터넷 중독 정도는 최초 인터넷 이용 시기, 인터넷 이용용도, 하루 인터넷 사용 시간, 부모컴퓨터 사용 여부에 따라 유의한 차이가 있었으며, 넷째, 인터넷 중독과 학교생활 적응과의 관계를 분석한 결과 학교생활 부적응인 경우는 '고위험군' 32명(58.2%), '잠재적위험군' 24명(50.0%), '정상군' 257명 (28.7%)으로 고위험군과 잠재적위험군이 정상군보다 학교생활 적응 정도가 낮은 것으로 조사되었다(p<0.01). 다섯째, 인터넷 중독에 유의한 영향을 주는 변수는 학교생활 적응 정도, 성별, 하루 인터넷 사용시간, 인터넷 이용용도, 인터넷 이용 상황, 어머니 직장 유무, 거주 지역, 부모 컴퓨터 사용여부, 최초 인터넷 이용시기이었다. 학교생활 적응 정도가 낮을 때, 남학생, 하루 인터넷 사용시간이 3시간 이상, 인터넷 이용 용도가 '게임', '스트레스가 쌓였을 때' 인터넷을 이용하는 경우, 어머니 직장이 있는 경우, 거주 지역이 동지역, 부모님이 컴퓨터를 사용할 줄 모르는 경우, 최초 인터넷 이용 시기가 취학 전인 경우가 인터넷 중독 점수가 높은 것으로 나타났다. 여섯째, 학교생활 적응에 유의한 영향을 주는 변수는 인터넷 중독 점수, 학교성적, 가정경제수준, 거주지역, 인터넷 이용용도, 학년이었다. 인터넷 중독 점수가 낮을수록, 학교성적이 높을수록, 가정경제수준이 잘 살수록, 거주지역이 동지역, 인터넷 이용용도가 '정보검색, 홈페이지관리/메신저', 5학년인 경우에 학교생활 적응을 더 잘하고 있는 것으로 나타났다. 이상의 결과를 종합해 보면, 학교생활 적응 정도가 낮을수록 인터넷 중독 점수가 높게 나타나고 있고, 인터넷 중독 경향이 높을수록 학교생활 적응 수준이 낮게 나타나고 있음을 알 수 있다. 아동들은 하루중 대부분의 시간을 학교에서 생활하고 있기 때문에 학교생활 적응은 아동들이 건전한 성인으로 성장하는데 중요한 요인이 된다. 또한, 인터넷사용률 증가와 최초 인터넷 이용 연령층이 점점 낮아지고 있음에 따라 인터넷 중독률이 자연스럽게 점점 높아질 것으로 예측되어진다. 따라서, 가정과 학교가 연계하여 아동들의 인터넷 사용에 대한 지속적인 관심과 지도가 필요하며, 인터넷 중독경향이 높은 아동들이 학교생활 적응을 잘할 수 있도록 인터넷 중독 치료 및 예방교육 프로그램 운영이 필요하다 하겠다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.141-148
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• 1992

Field experiment was carried out to investigate the effect of plant growth regulator (PGR); IAA, GA, Kinetin to regrowth of sorghum and pearlmillet according to variety and plant growth stage. Kinetin application after cut increased tiller number and decreased dry weight of regrowth, but its application on sorghum stubble in water stress increased tiller number and leaf elongation rate, consequently increased regrowth dry weight. GA application reduced tiller production in both species, but tiller formation in pearlmillet was decreased more than in sorghum by promoting leaf elongation of old tiller. Nonstructural carbohydrate (NSC) of stubble during regrowth was consumed less at anthesis than at stem elongation stage because of senescence of tiller primordia. GA treatment reduced NSC content more than other PGR in both plant species, by consuming reserve NSC and stimulating rapid elongation of old tiller after cut. Dry matter increase during regrowth had high correlation with tiller number and tiller elongation a week after cut, while it did not have any correlation with NSC at cutting stage or with consumption of NSC during regrowth. Therefore, regrowth in sorghum and pearmillet must depend upon activity of tiller primordia more than upon amount of reserved NSC.