• 대한한방부인과학회지
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• 제19권1호
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• pp.69-80
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• 2006

Purpose : This study is to evaluate the synergistic cytotoxicity of Rhizoma Rehmanniae(RR), in adriamycin-treated HeLa human cervical carcinoma cells. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% $CO_2$. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : The combination of RR and adriamycin synergistically augmented the cytotoxicity of HeLa cells. The apoptotic cell death was accompanied by the activation of caspase-3 and -8 as well as cleavage of poly(ADP- ribose) polymerase (PARP) in HeLa cells. The co-treatment of RR with adriamycin didn't have any effect on either the expression of Bcl-2 or that of Bax. Interestingly, a synergistic increase in apoptosis by the combination of two drugs was accompanied by the enhancement of Pas and Fas ligand (FasL) expression in HeLa cells. Taken together, the combination of RR and adriamycin significantly augmented the apoptotic cytotoxicity of Fas-positive cells, such as HeLa cells. The pathway is not involved in mitochondria-dependent pathway. Conclusion : RR induces apoptosis in HeLa cells via p38 MAPK activation.

• Journal of Pharmaceutical Investigation
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• 제26권1호
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• pp.43-53
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• 1996

The objective of this study was to determine whether 4-phenylazobezyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Pz-peptide), an enhancer of hydrophilic solute permeability in the intestine, could elevate the paracellular permeability of hydrophilic drugs across cornea and conjunctiva in the rabbit. The in-vitro penetration of hydrophilic drugs (mannitol, atenolol) and lipophilic drug (propranolol) across the rabbit cornea and conjunctiva was studied either in the presence or absence of 3 mM Pz-peptide. Drug penetration was evaluated using the modified Ussing chamber. The conjunctiva was more permeable than the cornea to all drugs. Pz-peptide showed enhanced effects on the drug transport across cornea and conjunctiva in a concentration dependent manner. Effects or ion transport inhibitor on the mannitol penetration were then investigated. Mannitol penetration was not changed by serosal addition of $100\;{\mu}M$ ouabain, suggesting that $Na^+/K^+$ ion tranporter was not involved in the Pz-peptide induced elevation of paracellular drug permeability. Furthermore, effects of Pz-peptide and EDTA on the transport of atenolol and propranolol into the ocular tissues or blood circulation after its administration into both eyes were investigated. EDTA showed enhanced effect on propranolol transport into the ocular tissues, but Pz-peptide did not show significant difference. Systemic absorption of propranolol by the addition of EDTA or Pz-peptide was not changed. On the other hand, EDTA and Pz-peptide elavated the atenolol transport into the ocular tissues. The transport of atenolol into the blood circulation was also enhanced by the addition of EDTA, but no effect was observed by the addition of Pz-peptide. The above findings suggest that Pz-peptide would be used as an paracellular pathway enahncer of hydrophilic drugs into the eye, without affecting the systemic absortion of topically applied opthalmic drugs.

• Journal of Pharmaceutical Investigation
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• 제25권4호
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• pp.353-363
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• 1995

The purpose of this study was to investigate the intestinal and nasal absorption enhancement of cefotaxime (CTX) by ion-pairing with counterions and to design an effective oral and intranasal drug delivery system for antibiotics. Counterions for absorption promotion were cationic surfactants [cetylpyridinium chloride (CP), cetrimide (CT) and benzalkonium chloride (BA)]. In the presence of counterions, the apparent partition coefficient of cefotaxime was increased depending on the molar concentration of the counterions. Anion interference was observed for ion-pairing of cefotaxime with counterions because of the counterbalance between an anion and counterions. The present study employed the in situ simultaneous nasal and intestinal perfusion technique in rats. The apparent permeabilities $(P_{app})$ of cefotaxime were $1.43{\pm}0.04{\times}10^{-5}\;cm/sec(mean{\pm}S.E)$ in the nasal cavity and 0 in the jejunum, respectively, which indicated that the intrinsic absorptivity of cefotaxime was greater in the nasal cavity than in the jejunum. When ionupairing formers were used, the decreasing order of apparent cefotaxime permeability $(P_{app},\;10^{-5}\;cm/sec)$, corrected for surface area of absorption, was as followings: $BA\;(7.50{\pm}0.36)\;>\;CT\;(4.92{\pm}0.24)\;>\;CP\;(3.01{\pm}0.17)$ in the jejunum and $BA\;(22.31{\pm}1.36)\;>\;CP\;(18.24{\pm}0.81)\;>\;CT \;(16.22{\pm}1.87)$ in the nasal cavity. The increase in permeability of cefotaxime was about 13-fold in the rat nasal cavity and was marked in the rat jejunum for ion-pairing with counterions as compared to those without ion-pairing. The damages of jejunal and nasal mucosal membrane by counterions were observed within approximately 2hrs after removal of ion-pair of cefotaxime with counterions from the nasal cavity and jejunum. These results suggest that CP can be used as an ion-pairing former in the jejunum and CP and CT can be used as ion-pairing formers in the nasal cavity for cefotaxime, as well as for poorly absorbed drugs with a negative charge due to ionization.

• Journal of Ginseng Research
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• 제19권3호
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• pp.225-230
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• 1995

The effect of long-term ginseng (Panax ginseng C.A. Meyer) administration on the clearance of carboxyhemoglobin (CO-Hb) and the property of blood gases was investigated in rats. Rats were received ginseng water extract (0.025% in drinking water) for 42 weeks starting at the age of 6 weeks. They were exposed to the diluted mainstream smoke generated from 15 filter cigarettes for 20 min in a round polycarbonate chamber (D37 cmXH13 cm). Under this condition, the mean CO-Hb content of control and the ginseng-treated rats immediately after the exposure was nearly the same as 13.8$\pm$2.9 f) and 13.9$\pm$1.6%, respectively. However, CO-Hb was more rapidly removed from blood in the ginseng treated rats than in untreatEd control with the laps of time, namely, its biological half life In the former was 36.9$\pm$1.5 min and in the latter was 56.9$\pm$13.2 min. Although long-term ginseng treatment did not affect the content of hemoglobin and blood pH of rats, it slightly increased blood oxygen content and its partial pressure value, and decreased levels of carbon dioxide and bicarbonate. These results suggest that long-term administration of rats with ginseng extract accelerate the elimination of CO from the blood. This effect seems to be related to the enhancement of oxygen consumption of the rat by a certain action of ginseng components as previously reported.

• 한국한의학연구원논문집
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• 제16권1호
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• pp.111-118
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• 2010

Objectives : We identified the applicability of Sasang constitution for the enhancement of job satisfaction, job performance and organizational commitment in office workers. Methods : We conducted a survey using a Sasang constitution questionnaire to identify the Sasang types, and several questionnaires to identify organizational commitment, job performance and job satisfaction of 205 office workers who live in D city. Data were analyzed with ANOVA test. Results 1. In organizational commitment, Sasang constitution showed a significant correlation in the question of "I feel that I am not a real member of this company" and the order of score was Soeumin, Taeeumin, and Soyangin. 2. In job performance, Sasang constitution showed significant correlations in the questions of "I always achieve my goals" and "I am recognized as a person who has a good job performance" and the order of score was Soeumin, Taeeumin, and Soyangin. 3. In job satisfaction, Sasang constitution showed a significant correlation in the questions of "I am forced to go to the office" and the order of score was Soeumin, Soyangin, and Taeeumin. Sasang constitution also showed significant correlations in the questions of "I am usually passionated in my work", and "I was disappointed when I got at my job" and the order of score was Soeumin, Taeeumin, and Soyangin. Conclusions : Sasang constitution. had significant correlation in organizational commitment, job performance and job satisfaction in office workers.

• 한국산학기술학회논문지
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• 제13권8호
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• pp.3477-3485
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• 2012

본 논문은 혁신클러스터 추진의 전략방향 설정을 위해서 클러스터 현황을 클러스터분석을 통하여 살펴보았다. 클러스터 분석은 클러스터 내에서 일어나는 다양한 형태의 협력을 파악하고 정책적인 대안의 도출을 위해 활용되는 실제적인 분석도구이다. 이에 본 논문에서는 광교테크노밸리의 발전단계에 따른 협력현황 분석을 위하여 산학연협력체계 분석과 진단을 위한 클러스터분석(Cluster Analysis)을 실시하였다. 클러스터 분석결과 광교테크노밸리내 입주기업의 산학연 협력경험은 67.3%로 매우 높은 것으로 나타났다. 대학과 기업과는 연구개발, 연구기관과는 장비활용 중심으로 협력하고 있었다. 또한, 산학연 협력의 지원정책수요는 협력기관 현황제공, 기술별 정보취득 지원 등 다양한 부문에서 협력의 수요가 존재하고 있었다. 이러한 분석결과를 토대로 혁신클러스터 구성단계를 넘어서 확장단계로 발전하기 위해서는 첫째, 혁신클러스터 발전을 위한 새로운 비전을 조속히 제시하고 단지인근에서 입주기업의 산학연협력의 활성화를 위한 지원을 추진해야 한다. 둘째, 혁신클러스터 단계별 발전을 위한 통합적인 지원역량 강화가 필요하다. 마지막으로 인근의 타 혁신거점과 정책적 네트워크 구축을 통해서 타 혁신클러스터의 자원과 역량을 활용할 수 있는 지원망 구축이 필요하다.

• 대한한의학방제학회지
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• 제27권1호
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• pp.45-52
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• 2019

Objectives : Hyperthermia is a widely used therapeutic tool for cancer therapy and a well-known inducer of apoptosis. Although the Cinnamomi cortex (CC) is a potent anticancer agent for several human carcinomas, it is less potent in the human U937 cell line. To explore any enhancing effects of CC with hyperthermia induced apoptosis, this study investigated the combined effects and apoptotic mechanisms of hyperthermia and CC in U937 cells. Methods : U937 cells were heat treated at $43^{\circ}C$ for 30 min with or without pre-treatment for 1h with CC and then incubated at $37^{\circ}C$ with 5% $CO_2$. Cell viability was analyzed by MTT assay and Trypan blue assay. Morphological changes reflecting apoptosis were visualized under microscope. Synergy effect of CC combined with hyperthermia were calculated by Compusyn software. The expression of proteins related to apoptosis and signaling pathways was determined by western blotting. Results : Hyperthermia with CC reduced cell viability and induced apoptosis. Combined hyperthermia and CC treatment markedly augmented apoptosis by upregulating proapoptotic proteins and suppressing antiapoptotic proteins, culminating in caspase-3 activation. Furthermore, the combined treatment, decreased the expression of in Bcl-2 family, cyclin D1, VEGF, MMP2 and MMP9 expression. Conclusion : This study provides compelling evidence that hyperthermia, in combination with CC, is a promising therapeutic strategy for enhancement of apoptosis and suggests a promising therapeutic approach for cancer.

• 한국연안방재학회지
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• 제4권4호
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• pp.189-196
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• 2017

Ocean Research Stations (ORSs) is the ocean platform type observation towers and measured oceanic, atmospheric and environmental data. These station located on the offshore area far from the coast, so they can produce the data without land effect. This study focused to improve the wave data quality of ORS station. The wave observations at ORSs are used by the C-band (5.8 GHz, 5.17 cm) MIROS Wave and Current Radar (MWR). MWR is convenient to maintenance and produce reliability wave data under bad weather conditions. MWR measured significant wave height, peak wave period, peak wave direction and 2D wave spectrum, so it's can provide wave information for researchers and engineers. In order to improve the reliability of MWR wave data, Datawell Waverider Buoy was installed near the one ORS (Socheoncho station) during 7 months and validate the wave data of MWR. This study found that the wave radar tend to be overestimate the low wave height under wind condition. Firstly, this study carried out the wave Quality Control (QC) using wind data, however the quality of wave data was limited. So, this study applied the four filters (Correlation Check, Direction Filter, Reduce White Noise and Phillips Check) of MWR operating software and find that the filters effectively improve the wave data quality. After applying 3 effective filters in combination, the RMSE of significant wave height decreased from 0.81m to 0.23m, by 0.58m and Correlation increased from 0.66 to 0.96, by 0.32, so the reliability of MWR significant wave height was significantly improved.

• ALGAE
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• 제39권1호
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• pp.31-41
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• 2024

Acadian marine plant extract powder (AMPEP) and Kelpak are commercial biostimulants derived from brown algae Ascophyllum nodosum. This study was to determine if AMPEP and Kelpak can induce thermal resistance in Pyropia yezoensis. P. yezoensis blades were exposed to different concentrations (control: 0, low: 0.001, high: 1 ppm) of AMPEP and Kelpak at 10℃ for 6 and 7 days, respectively. Those blades were then cultivated in von Stosch enriched seawater medium at different temperatures (10, 15, 20, and 25℃) with 12 : 12 L : D photoperiod and 100 µmol m^-2 s^-1 of photosynthetically active radiation for additional 15 days. Results showed that P. yezoensisreproduced archeospores at 20 and 25℃ at all biostimulant conditions within 15 days. At lower temperatures (10 and 15℃), only AMPEP-treated P. yezoensis reproduced archeospores. P. yezoensis exposed to 1 ppm Kelpak exhibited higher phycoerythrin and phycocyanin contents than control and 0.001 ppm conditions at 15℃. AMPEP-treated conditions showed higher phycoerythrin and phycocyanin contents than control at 10℃. These results suggest that AMPEP and Kelpak may not enhance the thermal resistance of P. yezoensis. However, AMPEP stimulated archeospores release at lower temperatures. The treatment of AMPEP and Kelpak also increased the pigment contents in P. yezoensis. These results suggest that the use of seaweed-derived biostimulants can provide some economic benefits in P. yezoensis aquaculture. The enhancement of archeospores formation by AMPEP at lower temperature may also increase the productivity since Pyropia farming relies on the accumulation of secondary seedings via asexual reproduction.

• Physical Therapy Rehabilitation Science
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• 제13권2호
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• pp.152-162
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• 2024

Objective: This study was conducted to analyze the effect of wearable Electromyography-controlled functional electrical stimulation (EMG-controlled FES) System on Gait Function and cardiopulmonary metabolic efficiency during walking in older adults. Design: Cross-section study Methods: Total 22 older adult participants suitable to selection criteria of this study participated in this study. The EMG-controlled FES System, which functions as a wearable physical activity assist FES system was used. All participations performed randomly assigned two conditions (Non-FES assist [NFA], FES assist [FA]) of walking. In all conditions, spatio-temporal parameters and kinematics and kinetics parameters during walking was collected via 3D motion capture system and 6 minutes walking test (6MWT) and metabolic cost during walking and stairs climbing was collected via a portable metabolic device (COSMED K5, COSMED Srl, Roma, Italy). Results: In Spatio-temporal parameters aspects, The EMG-controlled FES system significantly improved gait functions measurements of older adults with sarcopenia at walking in comparison to the NFA condition (P<0.05). Hip, knee and ankle joint range of motion increased at walking in FA condition compared to the NFA condition (P<0.05). In the FA condition, moment and ground reaction force was changed like normal gait during walking of older adults in comparison to the NFA condition (P<0.05). The EMG-controlled FES system significantly reduced net cardiopulmonary metabolic energy cost, net energy expenditure measurement at stairs climbing (P<0.05). Conclusions: This study demonstrated that EMG-controlled FES is a potentially useful gait-assist system for improving gait function by making joint range of motion and moment properly.