• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.3
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• pp.239-252
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• 2024

Recombination activating genes (RAGs) play a crucial role in initiating V(D)J recombination, which is essential for developing adaptive immunity in vertebrates. In this study, we cloned and characterized RAG1/2 cDNA from the marine medaka Oryzias dancena (OdRAG1/2) and investigated their mRNA expression patterns during ontogenetic developmental stages. The OdRAG1 and OdRAG2 cDNA contained open reading frames (ORFs) encoding proteins containing 1,078 and 531 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that OdRAG1 and OdRAG2 are highly conserved with their corresponding orthologs, featuring distinct core and non-core regions. Notably, expression analysis showed that, in contrast to other fish RAGs studied, OdRAG1/2 expression peaked at 0 days post-hatching (DPH). Additionally, for the expression of T and B cell differentiation markers, CD3γ and CD20, also peaked at 0 DPH. Collectively, adaptive immunity in O. dancena potentially begins during embryonic development, which is critical for V(D)J recombination and essential immune component development, suggesting the early ontogenetic stage interactions between innate and adaptive immunity.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.2
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• pp.236-248
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• 2019

We investigated occurrence and molecular phylogeny of the toxic epiphytic dinoflagellate Ostreopsis at seven sampling sites in the coastal waters off Jeju Island of Korea in April, 2017. During the sampling period, surface water temperature ranged from 15.7 to $18.3^{\circ}C$ and salinity was relatively constant, ranging from 33.4 to 34.9. Of a total of 13 macroalgal species collected from all sampling sites, Ostreopsis cells were observed from 8 macroalgal species and the highest cell abundance ($157.5cells\;g^{-1}$) was recorded on the red alga Grateloupia filicina at St. 6. LSU rDNA D8/D10 sequences of all Korean Ostreopsis strains isolated from the 4 sampling sites were 100% identical. Molecular phylogentic analyses (BI and ML) inferred from LSU rDNA alignment showed that the Korean Ostreopsis strains placed into the previously described the Ostreopsis sp. 1 clade, which contained strains isolated from the temperate coastal waters of Japan. The Korean Ostreopsis sp. 1 strain grew in a wide range of temperature ($10-30^{\circ}C$) and salinity (25-30), with its maximum growth rate of $0.49d^{-1}$ at $25^{\circ}C$ and salinity of 30, indicating that they can be tolerated in temperate areas.

• 전자공학회논문지 IE
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• v.44 no.1
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• pp.27-32
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• 2007

This study was to suggest the predictive model of propagation, considering the effect by the multipath waves produced by the sending and receiving vehicles' left/right reflectors and the adjacent vehicles when the communication between the vehicles on the one-way two-lanes road in the urban city with a lot of traffic jams. Then, the radius of curved road was 600[m], the length of curved roads $52.4\sim471.2[m]$, and the bridge's pier of road was $5o\sim45o$. Also, it was simulated by changing the receiving vehicle located on the curved road's gap from minimum 3.3[m] to maximum 29.5[m], corresponding to the change of distance of the bridge's pier of road and curved road. As a result of this research above, in case of $5o\sim15o$ bridge's pier of road, it was within l[dB] regardless of the receiving vehicle's position on the curved road in case of propagation path loss. In case of $15o\sim45o$, it was approximately $1\sim8[dB]$ as the bridge's pier of road is changed. And, in case of propagation path, it found out that it was changed to $0.4\sim120[m]$ according to the change of bridge's pier of road. Then, the delay time of propagation was 400[nsec] as it produced 120[m] in the difference of propagation path.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.4
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• pp.894-900
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• 2004

There are many types of circularly polarized(CP) microstrip antenna elements, which are used as a good unit radiator. Since an advantage of CP antenna is no strict alignment requirements between Rx and Tx system, the printed antennas with circular polarization are very often used in numerous satellite and mobile radio systems. In order to realize the broad bandwidth of 3 dB axial ratio and impedance of CP microstrip antenna, complex feed structure and tri-plate patch element have been researched. This paper describes a design of wideband microstrip cross slot array antennas with circular polarization. The proposed antenna is composed of an open-ended microstrip feed line as a feeder and a cross slot as a radiator for circular polarization. To realize the wide bandwidth, tri-plate structure are considered and cross slot is electromagnetically coupled with feed line. Optimum parameters of 1-element cross slot antenna are analyzed and designed by method of moments. These parameters are also applied to may antennas design considered the mutual coupling between radiating elements. Right hand circular polarization(RHCP) and left hand circular polarization(LHCP) of the proposed antenna are easily controlled by asymmetrical cross slot structure and slot position. In 1-element and 15-element cross slot array antenna, the good axial ratio of 1 dB below and the broad bandwidth characteristics of antenna are obtained.

• The Plant Pathology Journal
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• v.25 no.1
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• pp.47-53
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• 2009

Sequence analysis of the Chlorella virus SS-2 revealed one putative nuclease gene that is 807 bp long and encodes a 31kDa protein. Multiple sequence alignment analysis reveals the presence of highly conserved PD-(D/E)XK residues in the encoded protein. The gene cloned into an expression vector was expressed as a His-tagged fusion protein in chaperone containing pKJE7 cells. The recombinant protein was purified using a His-Trap chelating HP column and used for functional analysis. Exonuclease activity of the SS-2 nuclease was detected when the DNA substrates, such as linear ssDNA, PCR amplicon, linear dsDNA with 5'-overhang ends, 3'-overhang ends, or blunt ends were used. Covalently closed circular DNA was also degraded by the SS-2 recombinant protein, suggesting that the SS-2 nuclease has an endonuclease activity. Stable activity of SS-2 nuclease was observed between $10^{\circ}C$ and $50^{\circ}C$. The optimum pH concentrations for the SS-2 nuclease were pH 6.0-8.5. Divalent ions inhibited the SS-2 nuclease activity.

• The Journal of the Korean dental association
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• v.54 no.2
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• pp.134-141
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• 2016

Use of ready-made orthodontic appliance can lead to inefficiencies in the final stages of the orthodontic treatment. Because patients' teeth have anatomic variations, brackets that have been designed to fit on average tooth surface may result in positional discrepancies when leveling and alignment is completed. As a result, additional steps such as rebonding, wire bending and use of auxiliaries may be needed. Even in patients who have normal tooth anatomy and proper tooth size relationships, precise bracket placement is crucial in order to efficiently control the tooth positions. Digital models can provide advantages in clinical orthodontics as virtual tooth setup could be performed, and clinicians can easily visualize the predicted final occlusion. Through this setup model, customized brackets with individualized prescription and archwires that optimally fit with the patients' dental arches can be produced using CAD/CAM technology. Also, the brackets can be accurately placed with an aid of 3D-printed jigs. The purpose of this article is to introduce the commonly used labial and lingual customized orthodontic appliance systems using digital technology.

• Journal of the Korea Society of Computer and Information
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• v.23 no.4
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• pp.57-64
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• 2018

In this paper, we propose the automatic detection method for individual region separation using panorama image. Finding areas that contain individual teeth is one of the most important tasks in automating 3D models through individual tooth separation. In the conventional method, the maxillary and mandibular teeth regions are separated using a straight line or a specific CT slide, and the tooth regions are separated using a straight line in the vertical direction. In the conventional method, since the teeth are arranged in a curved shape, there is a problem that each tooth region is incorrectly detected in order to generate an accurate tooth region. This is a major obstacle to automating the creation of individual tooth models. In this study, we propose a method to find the correct tooth curve by using the jawbone curve which is very similar to the tooth curve in order to overcome the problem of finding the area containing the existing tooth. We have proposed a new method to accurately set individual tooth regions using the feature that individual teeth are arranged in a direction similar to the normal direction of the tooth alignment curve. In the proposed method, the maxillary and mandibular teeth can be more precisely separated than the conventional method, and the area including the individual teeth can be accurately set. Experiments using real dental CT images demonstrate the superiority of the proposed method.

• Korean Journal of Optics and Photonics
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• v.12 no.5
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• pp.362-370
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• 2001

We prove that 3-D profile mapping using white light interferometry has systematic errors caused by aberrations of the optimal system. The tilt of an object invokes an offset between the object and the reference ray, which eventually makes the aberration cancel incompletely. The fringe peak of a white light interferogram is mainly affected by the aberration effect while the envelope peak remains stable. By the difference between the two peaks, it is easily confirmed how much the error existed in the fringe peak. Experimental results prove that the error caused by aberration is varied by object tilt, microscope NA, optics alignment within the range of $\pm$50nm.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2003.09a
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• pp.261-278
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• 2003

Concepts.Wafer-Level Chip-Scale Concept with Handling Substrate.Low Accuracy Placement Layout with Isolation Trench.Possible Pitch of Interconnections down to $10{\mu}{\textrm}{m}$ (Sn-Grains).Wafer-to-Wafer Equipment Adjustment Accuracy meets this Request of Alignment Accuracy (+/-1.5 ${\mu}{\textrm}{m}$).Adjustment Accuracy of High-Speed Chip-to-Wafer Placement Equipment starts to meet this request.Face-to-Face Modular / SLID with Flipped Device Orientation.interchip Via / SLID with Non-Flipped Orientation SLID Technology Features.Demonstration with Copper / Tin-Alloy (SLID) and W-InterChip Vias (ICV).Combination of reliable processes for advanced concept - Filling of vias with W as standard wafer process sequence.No plug filling on stack level necessary.Simultanious formation of electrical and mechanical connection.No need for underfiller: large area contacts replace underfiller.Cu / Sn SLID layers $\leq$ $10{\mu}{\textrm}{m}$ in total are possible Electrical Results.Measurements of Three Layer Stacks on Daisy Chains with 240 Elements.2.5 Ohms per Chain Element.Contribution of Soldering Metal only in the Range of Milliohms.Soldering Contact Resistance ($0.43\Omega$) dominated by Contact Resistance of Barrier and Seed Layer.Tungsten Pin Contribution in the Range of 1 Ohm

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.68.2-68.2
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• 2020

UVO-Multiband Polarizing Imager System (UVOMPIS) is an ultraviolet to visible light multi-wavelength polarization/imaging system for Compact Advanced Satellite. We developed Linear Astigmatism Free-Three Mirror System (LAF-TMS) D200F2 as an optical system of UVOMPIS which has an entrance pupil diameter of 200 mm, a focal ratio of 2, a field of view of 2° × 4°. LAF-TMS is a confocal off-axis reflecting telescope system that removes linear astigmatism, and its all mirrors (M1, M2, M3) are optimized with the freeform surface to reduce high-order aberrations. Through the sensitivity analysis and Monte-Carlo simulation as the tolerance analysis, we can confirm the feasibility of the system, relatively sensitive parameters (tilt, decenter, despace, surface RMS error), and considerations for optomechanical design. From the sensitivity analysis, we can discover the relatively sensitive optical alignment parameters to a single perturbation. Further more, in the monte-carlo simulation, we investigate the minimum tolerance budget satisfying the required optical performance and whether the tolerance range is satisfied within manufacturing error.