• Title/Summary/Keyword: 3D Cell Printing

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Stem cell attached 3-dimentional printed polycarprolactone scaffold (줄기세포 탑재 3차원 프린팅 polycarprolactone 스캐폴드)

  • Hong, Gyusik;Cho, Jeong Hwan;Yun, Seokhwan;Choi, Eunjeong;An, Seongmin;Kim, Jung Seok;Lee, Jae Sam;Shim, Jin-hyung;Jin, Songwan;Yun, Won-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.8
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    • pp.618-626
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    • 2019
  • Stem cell therapy is not expected to bestow any therapeutic benefit because of the low engraftment rates after transplantation.Various cell-carrying scaffolds have been developed in order to overcome this problem. When the scaffold is formed by 3-dimensional (3D) printing, it is possible to create various shapes of scaffolds for specific regions of injury. At the same time, scaffolds provide stem cells as therapeutic-agents and mechanically support an injured region. PCL is not only cost effective, but it is also a widely used material for 3D printing. Therefore, rapid and economical technology development can be achieved when PCL is printed and used as a cell carrier. Yet PCL materials do not perform well as cell carriers, and only a few cells survive on the PCL surface. In this study, we tried to determine the conditions that maximize the cell-loading capacity on the PCL surface to overcome this issue. By applying a plasma treated condition and then collagen coating known to improve the cell loading capacity, it was confirmed that the 3% collagen coating after plasma treatment showed the best cell engraftment capacity during 72 hours after cell loading. By applying the spheroid cell culture method and scaffold structure change, which can affect the cell loading ability, the spheroid cell culture methods vastly improved cell engraftment, and the scaffold structure did not affect the cell engraftment properties. We will conduct further experiments using PCL material as a cell carrier and as based the excellent results of this study.

Microfluidic Device for Ultrasound Image Analysis based on 3D Printing (초음파 영상 분석을 위한 3D 프린팅 기반 미세유체소자)

  • Kang, Dongkuk;Hong, Hyeonji;Yeom, Eunseop
    • Journal of the Korean Society of Visualization
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    • v.16 no.1
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    • pp.15-20
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    • 2018
  • For the measurement of biophysical properties related with cardiovascular diseases (CVD), various microfluidic devices were proposed. However, many devices were monitored by optical equipment. Ultrasound measurement to quantify the biophysical properties can provide new insights to understand the cardiovascular diseases. This study aims to check feasibility of microfluidic device for ultrasound image analysis based on 3D printer. To facilitate acoustic transmission, agarose solution is poured around 3D mold connected with holes of the acrylic box. By applying speckle image velocimetry(SIV) technique, flow information in the bifurcated channel was estimated. Considering that ultrasound signal amplitude is determined by red blood cell (RBC) aggregation, RBC aggregation in the bifurcated channel can be estimated through the analysis of ultrasound signal. As examples of microfluidic device which mimic the CVD model, velocity fields in microfluidic devices with stenosis and aneurysm were introduced.

Fabrication of Calcium Phosphate Scaffolds Using Projection-based Microstereolithography and Their Effects on Osteogenesis (투영기반 마이크로 광조형 기술을 이용한 3 차원 인산칼슘 인공지지체 제작 및 골 분화 영향)

  • Seol, Young-Joon;Park, Ju-Young;Cho, Dong-Woo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.11
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    • pp.1237-1242
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    • 2011
  • Calcium phosphates are very interesting materials for use as scaffolds for bone tissue engineering. These materials include hydroxyapatite (HA) and tricalcium phosphate (TCP), which are inorganic components of human bone tissue and are both biocompatible and osteoconductive. Although these materials have excellent properties for use as bone scaffolds, many researchers have used these materials as additives to synthetic polymer scaffolds for bone tissue regeneration, because they are difficult to manufacture three-dimensional (3D) scaffolds. In this study, we fabricated 3D calcium phosphate scaffolds with the desired inner and outer architectures using solid freeform fabrication technology. To fabricate the scaffold, the sintering behavior was evaluated for various sintering temperatures and slurry concentrations. After the fabrication of the calcium phosphate scaffolds, in-vitro cell proliferation and osteogenic differentiation tests were carried out.

Fabrication Method of OPV using ESD Spray Coating (ESD 스프레이를 이용한 OPV 제작 기법)

  • Kim, Jungsu;Jo, Jeongdai;Kim, Dongsoo
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.84.2-84.2
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    • 2010
  • PEMS (printed electro-mechanical system) is fabricated by means of various printing technologies. Passive and active components in 2D or 3D such as conducting lines, resistors, capacitors, inductors and TFT, which are printed with functional materials, can be classified in this category. And the issue of PEMS is applied to a R2R process in the manufacturing process. In many electro-devices, the vacuum process is used as the manufacturing process. However, the vacuum process has a problem: it is difficult to apply toa continuous process as a R2R printing process. In this paper, we propose an ESD (electro static deposition) printing process has been used to apply an organic solar cell of thin film forming. ESD is a method of liquid atomization by electrical forces, anelectrostatic atomizer sprays micro-drops from the solution injected into the capillary, with electrostatic force generated by electric potential of about tens of kV. ESD method is usable in the thin film coating process of organic materials and continuous process as a R2R manufacturing process. Therefore, we experiment the thin films forming of PEDOT:PSS layer and Active layer which consist of the P3HT:PCBM. The result of experiment, organic solar cell using ESD thin film coated method is occurred efficiency of about 1.4%. Also, the case of only used to ESD method in the active layer coating is occurred efficiency of about 1.86% as the applying a spin coating in the PEDOT:PSS layer. We can expect that ESD method is possible for continuous process to manufacture in the organic solar cell or OLED device.

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A Study on the Simulation of Ink Penetration into the Uncoated Papers in Gravure Printing (그라비어 인쇄에서 비도피지의 잉크 침투 시뮬레이션에 관한 연구)

  • Seo, Yea-Ri;Youn, Jong-Tae
    • Journal of the Korean Graphic Arts Communication Society
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    • v.28 no.1
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    • pp.1-13
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    • 2010
  • Gravure printing and manufacturing of advanced electronic components in a way that is going extra hold position. It is to print the electronic components of the rapid productivity improvements as well as cost-saving and environment-friendly industries such as the transition is a big advantage. However the mechanism of gravure is difficult to study scientifically because of high speed and excessively small size of the cell. To approach the mechanism we experimented using gravure printability. The condition of variables of IGT is pressure and velocity. By using Flow-3D simulation software, we built up the theoretical model under the constant variables. Then, we compared the real test with the simulation results. Therefore, it is studied the mechanism of gravure scientifically and it can be analysed the effect of the variable conditions.

Development of Biomimetic Scaffold for Tissue Engineering (조직공학을 위한 생체모사용 스캐폴드 개발)

  • Park, Su-A;Lee, Jun-Hee;Kim, Wan-Doo
    • Elastomers and Composites
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    • v.44 no.2
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    • pp.106-111
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    • 2009
  • Tissue engineering is a research field for artificial substitutes to improve or replace biological functions. Scaffolds play a important role in tissue engineering. Scaffold porosity and pore size provide adequate space, nutrient transportation and cell penetration throughout the scaffold structure. Scaffold structure is directly related to fabrication methods. This review will introduce the current technique of 3D scaffold fabrication for tissue engineering. The conventional technique for scaffold fabrication includes salt leaching, gas foaming, fiber bonding, phase seperation, melt moulding, and freeze drying. These conventional scaffold fabrication has the limitations of cell penetration and interconnectivity. In this paper, we will present the solid freeform fabrication (SFF) such as stereolithography (SLA), selective laser sintering (SLS), and fused deposition modeling (FDM), and 3D printing (3DP).

A study on the injection mold design application method of CAE mold analysis data (CAE 성형해석 데이터의 사출금형 설계 활용 방법에 관한 연구)

  • Nam, Seung-Don
    • Design & Manufacturing
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    • v.13 no.3
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    • pp.29-34
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    • 2019
  • Cell phone injection is characterized by its small size and thinness. In addition, the product has a short cycle time, requiring a very short production schedule. To produce more accurate products faster, data from experience in producing similar products is required. In this study, two mobile phone models are presented. In this study, the quality problems caused by molding analysis and actual injection molding were analyzed and made into a database. As a result, it was considered that all the defects in the molding analysis do not affect the product in some cases, rather than appear as defects in the actual product. All defects shown in the molding analysis can be made into a database, and based on this data, it will be possible to obtain an effect that can predict more accurately whether it will cause problems after injection.

Advances in Crystalline Silicon Solar Cell Technology

  • Lee, Hae-Seok;Park, Hyomin;Kim, Donghwan;Kang, Yoonmook
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.82-82
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    • 2015
  • Industrial crystalline silicon (c-Si) solar cells with using a screen printing technology share the global market over 90% and they will continue to be the same for at least the next decade. It seems that the $2^{nd}$ generation and the $3^{rd}$ generation technologies have not yet demonstrated competitiveness in terms of performance and cost. In 2014, new world record efficiency 25.6% (Area-$143.7cm^2$, Voc-0.740V, $Jsc-41.8mA/cm^2$, FF-0.827) was announced from Panasonic and its cell structure is Back Contact $HIT^*$ c-Si solar cell. Here, amorphous silicon passivated contacts were newly applied to back contact solar cell. On the other hand, 24.9% $TOPCon^{**}$ cell was announced from Fraunhofer ISE and its key technology is an excellent passivation quality applying tunnel oxide (<2 nm) between metal and silicon or emitter and base. As a result, to realize high efficiency, high functional technologies are quite required to overcome a theoretical limitation of c-Si solar cell efficiency. In this presentation, Si solar cell technology summarized in the International Technology Roadmap for Photovoltaics ($^{***}ITRPV$ 2014) is introduced, and the present status of R&D associated with various c-Si solar cell technologies will be reviewed. In addition, national R&D projects of c-Si solar cells to be performed by Korea University are shown briefly.

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Studies on the Width of Rectangular Channels of Fuel Cell Bipolar Plate Using FDM 3D Printer with PLA Filament

  • Kim, Jae-Hyun;Jin, Chul-Kyu
    • Journal of the Korean Society of Industry Convergence
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    • v.24 no.6_1
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    • pp.683-691
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    • 2021
  • Bipolar plates with channel width of 0.5 mm, 0.4 mm, and 0.3 mm respectively were printed using a 3D printer. The shape of three b ipolar plates was rectangular, the channel depth was 0.5 mm, and the thickness of base was 0.5 mm. The bipolar plate with channel width of 0.5 mm had 45 channels, and their active area was 44.5 mm × 50 mm. The bipolar plate with channel width of 0.4 mm had 57 channels and its active area was 45.2 mm × 50 mm, and the bipolar plate with channel width of 0.3 mm had 75 channels and its active area was 44.7 mm × 50 mm. The bipolar plates were printed using PLA filament. The cross-sectional lengths of the bipolar plates with channel widths of 0.5 mm and 0.4 mm were identical by 96% of the designed cross-sectional length. Whereas the bipolar plate with a channel length of 0.3 mm had a large difference of 25% from the designed cross-sectional length.

3D Printed Water Strider Robot with Environmental Monitoring (환경모니터링이 가능한 3D 프린팅 소금쟁이 로봇)

  • Shim, Ga-hyun;Lee, Kihak;Chun, Kyunghan;Cho, Chanseob;Kim, Bonghwan
    • Journal of Sensor Science and Technology
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    • v.28 no.6
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    • pp.407-413
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    • 2019
  • Using 3D printing technology, we created a biomimetic water strider robot that can monitor environments. We found ways to increase the bearing capacity of the fluid-driven water strider robot by conducting experiments then comparing with more stable robots. The controller of the robot is based on Arduino, and can be controlled wirelessly with a Bluetooth module. The speed of the robot is 7.37 cm/s, and the bearing capacity is 29 g. A lithium polymer battery that can be charged with a solar cell was used as a power source, and both the charging and driving times were also explored.