• Korean Journal of Applied Biomechanics
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• 제15권4호
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• pp.169-179
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• 2005

In the 500m short track speed skating, the matter of who reaches the first corner first can important factor since each competitor races with all speed from the start to the first line. A filed study was attempted to kinematical estimation six female foreign skaters, who participated in the 500m female final round competition, and two Korea skates during the World Short track Skating Championship. The three dimensional motion analysis with DLT method was executed using four video cameras for analyzing the actual competition situation. In point of analyzing the actual competition situation, it is expected that skaters and coaches the effective informations, and the following conclusions are drawn; The elapsed time by phase in start motion of the foreign skaters appeared shorter those of Korea skaters, so the start training of Korea skaters should be strengthed. Also the displacement of C.G in the foreign skaters appeared shorter displacement than those of Korea skaters. Especially in the starting position, the foreign skaters are superior to Korea skaters in displacement of first(left) and next following stroke(right). The velocity of C.G and maximum velocity of skate blade of foreign skaters art faster than those of Korea skaters. And the foreign skaters show the superior early velocity change. Both of leaning body angle, and left and knee angle of the foreign skaters lead to positive point of having the propulsive force in the early starting position. Observing in the most prominent feature of foreign and Korea skaters in start phase, foreign skaters skate quickly the third stroke. These features of Korea skaters would appear disadvantage of location selection in entering the coner course.

• Korean Journal of Applied Biomechanics
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• 제20권2호
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• pp.239-243
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• 2010

The putting stroke accounts for 40~50% of total stroke for a golf rounding and most golfers have difficulties on the puting. Studies for the putting stroke have been conducted by analyzing various factors such as kinematics, kinetics, psychologic and physiologic parameters. A lot of devices were developed to support the studies. However there was no appropriate method to measure the position of the ball quantitatively. In this study, we developed a new measurement system to measure and evaluate the putting result. The developed system uses a USB camera to take the 2-dimensional image of the surface including the hole cup at the center of the image and the ball. The position of the ball is extracted as a set of distance and angle in polar coordinate system. We evaluated the new system with an indoor set-up for putting experiments and the system provided accurate measurement results. The proposed system can be combined with the other measurement systems such as 3D motion capture system and force plate without any restriction.

• Korean Journal of Microbiology
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• 제29권1호
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• pp.49-55
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• 1991

The heat shock responses of Campylobacter jejuni were studied by examination of their survival rates and synthesis of heat shocd proteins. When C. jejuni cells were treated at the sublethal temperatures of 48.deg.C for 30 minutes, most of the cells maintained their viabilities and synthesized the heat shock proteins of 90, 73, and 66 kD in molecular weight. By the method of two-dimensional electrophoresis, the heat shock proteins of C. jejuni were identified to be Hsp90, Hsp73, and Hsp66. During the heat shock at 48.deg.C, the heat shock proteins were induced from about 5 minutes after the heat shock treatment. Their synthesis was continued upto 30 minutes, but remarkably retarded after 50 minutes. When C. jejune cells were heat shocked at 51.deg.C for 30 minutes, the survival rates of the cells were decreased by about $10^{3}$ fold and synthesis of heat shock proteins and normal proteins was also generally retarded. The cells exposed to 55.deg.C for 30 minutes died off by more than $10^{5}$ cells and the new protein synthesis was not observed. But when C. jejuni cells were heat-shocked at the sublethal temperature of 48.deg.C for 15 to 20 minutes and then were exposed at the lethal temperature of 55.deg.C for 30 minutes, their viabilities were higher than those exposed at 55.deg.C for 30 minutes without pre-heat shock at 48.deg.C. Therefore, the heat shock proteins synthesized at the sublethal temperature of 48.deg.C in C. jejuni were thought to be responsible for thermotolerance. However, when C. jejuni cells heat-shocked at various ranges of sublethal and lethal temperatures were placed back to the optimum temperature of 42.deg.C, the multiplication patterns of the cells pretreated at different temperatures were not much different each other.

• Journal of Biomedical Engineering Research
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• 제30권3호
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• pp.221-225
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• 2009

The purpose of this study was to investigate the biomechanical effects of an application of whole body vibration during strengthening exercise. Every participant performed four weeks exercise program using general leg-press versus vibrating leg-press. Participants did legpress exercise three sets of 25 repetitions with the load of 25 percent of 1RM during first week, three sets of 20 repetitions with 40 percent of 1RM during second week, three sets of 15 repetitions with 60 percents of 1RM during third week, and three sets of 15 repetitions with 80 percent of 1RM during last fourth week. The vibration(25Hz, 5mm) was applied only to the vibration exercise group. A three dimensional virtual lower extremity model for one of subject and virtual leg-press model were generated. The knee extensor muscle forces were analyzed using the virtual model and the knee joint torque(maximum extension torque) was measured using an isokinetic device. Calculated muscle forces were smaller in vibrating leg-press exercise than in general leg-press exercise. An increase of the maximum knee extension joint torque was 2.14 times larger approximately after the four week vibration leg-press exercise program was performed.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.925-935
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• 2014

A cold-adapted carbohydrate esterase, CEST, belonging to the carbohydrate esterase family 6, was cloned from Microbulbifer thermotolerans DAU221. CEST was composed of 307 amino acids with the first 22 serving as a secretion signal peptide. The calculated molecular mass and isoelectric point of the mature enzyme were 31,244 Da and pH 5.89, respectively. The catalytic triad consisted of residues Ser37, Glu192, and His281 in the conserved regions: GQSNMXG, QGEX(D/N), and DXXH. The three-dimensional structure of CEST revealed that CEST belongs to the ${\alpha}/{\beta}$-class of protein consisted of a central six-stranded ${\beta}$-sheet flanked by eight ${\alpha}$-helices. The recombinant CEST was purified by His-tag affinity chromatography and the characterization showed its optimal temperature and pH were $15^{\circ}C$ and 8.0, respectively. Specifically, CEST maintained up to 70% of its enzyme activity when preincubated at $50^{\circ}C$ or $60^{\circ}C$ for 6 h, and 89% of its enzyme activity when preincubated at $70^{\circ}C$ for 1 h. The results suggest CEST belongs to group 3 of the cold-adapted enzymes. The enzyme activity was increased by $Na^+$ and $Mg^{2+}$ ions but was strongly inhibited by $Cu^+$ and $Hg^{2+}$ ions, at all ion concentrations. Using p-nitrophenyl acetate as a substrate, the enzyme had a $K_m$ of 0.278 mM and a $k_{cat}$ of $1.9s^{-1}$. Site-directed mutagenesis indicated that the catalytic triad (Ser37, Glu192, and His281) and Asp278 were essential for the enzyme activity.

• Archives of Pharmacal Research
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• 제29권1호
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• pp.50-58
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• 2006

Translationally controlled tumor protein (TCTP), also known as histamine releasing factor (HRF), is found abundantly in different eukaryotic cell types. The sequence homology of TCTP between different species is very high, belonging to the MSS4/DSS4 superfamily of proteins. TCTP is involved in both cell growth and human late allergy reaction, as well as having a calcium binding property; however, its primary biological functions remain to be clearly elucidated. In regard to many possible functions, the TCTP of Plasmodium falciparum (Pf) is known to bind with an antimalarial agent, artemisinin, which is activated by heme. It is assumed that the endoperoxide-bridge of artemisinin is opened up by heme to form a free radical, which then eventually alkylates, probably to the Cys14 of PfTCTP. Study of the docking of artemisinin with heme, and subsequently with PfTCTP, was carried out to verify the above hypothesis on the basis of structural interactions. The three dimensional (3D) structure of PfTCTP was built by homology modeling, using the NMR structure of the TCTP of Schizosaccharomyces pombe as a template. The quality of the model was examined based on its secondary structure and biological function, as well as with the use of structure evaluating programs. The interactions between artemisinin, heme and PfTCTP were then studied using the docking program, FlexiDock. The center of the peroxide bond of artemisinin and the Fe of heme were docked within a short distance of $2.6{\AA}$, implying the strong possibility of an interaction between the two molecules, as proposed. When the activated form of artemisinin was docked on the PfTCTP, the C4-radical of the drug faced towards the sulfur of Cys14 within a distance of $2.48{\AA}$, again suggesting the possibility of alkylation having occurred. These results confirm the proposed mechanism of the antimalarial effect of artemisinin, which will provide a reliable method for establishing the mechanism of its biological activity using a molecular modeling study.

• Imaging Science in Dentistry
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• 제40권4호
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• pp.179-185
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• 2010

Purpose : Cortical bone thickness is one of the important factor in mini-implant stability. This study was performed to investigate the buccal cortical bone thickness at every interdental area as an aid in planning mini-implant placement. Materials and Methods : Two-dimensional slices at every interdental area were selected from the cone-beam computed tomography scans of 20 patients in third decade. Buccal cortical bone thickness was measured at 2, 4, and 6 mm levels from the alveolar crest in the interdental bones of posterior regions of both jaws using the plot profile function of $Ez3D2009^{TM}$ (Vatech, Yongin, Korea). The results were analyzed using by Mann-Whitney test. Results : Buccal cortical bone was thicker in the mandible than in the maxilla. The thickness increased with further distance from the alveolar crest in the maxilla and with coming from the posterior to anterior region in the mandible (p<0.01). The maximum CT value showed an increasing tendency with further distance from the alveolar crest and with coming from posterior to anterior region in both jaws. Conclusion : Interdental buccal cortical bone thickness varied in both jaws, however our study showed a distinct tendency. We expect that these results could be helpful for the selection and preparation of mini-implant sites.

• Journal of the Korea Institute of Information and Communication Engineering
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• 제18권6호
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• pp.1355-1360
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• 2014

In this paper, we apply the learning of the mobile robot insect augmented reality Digilog Book. In the era of electronic, book written in paper space just have moved to virtual reality space. The virtual reality, constraints spatial and physical, in the real world, it is a technique that enables to experience indirectly situation not experienced directly as user immersive experience type interface. Applied to the learning robot Digilog Book that allows the fusion of paper analog and digital content, using the augmented reality technology, to experience various interactions. Apply critical elements moving, three-dimensional images and animation to enrich the learning, for easier block assembly, designed to grasp more easily rank order between the blocks. Anywhere at any time, is capable of learning of the robot in Digilog Book to be executed by the mobile phone in particular.

• Steel and Composite Structures
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• 제1권4호
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• pp.427-440
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• 2001

The growing use of unprotected or partially protected steelwork in buildings has caused a lively debate regarding the safety of this form of construction. A good deal of recent research has indicated that steel members have a substantial inherent ability to resist fire so that additional fire protection can be either reduced or eliminated completely. A performance based philosophy also extends the study into the effect of structural continuity and the performance of the whole structural totality. As part of the structural system, thermal expansion during the heating phase or contraction during the cooling phase in most beams is likely to be restrained by adjacent parts of the whole system or sub-frame assembly due to compartmentation. This has not been properly addressed before. This paper describes an experimental programme in which unprotected steel beams were tested under load while it is restrained between two columns and additional horizontal restraints with particular concern on the effect of catenary action in the beams when subjected to large deflection at very high temperature. This paper also presents a three-dimensional mathematical modelling, based on the finite element method, of the series of fire tests on the part-frame. The complete analysis starts with an evaluation of temperature distribution in the structure at various time levels. It is followed by a detail 3-D finite element analysis on its structural response as a result of the changing temperature distribution. The principal part of the analysis makes use of an existing finite element package FEAST. The effect of columns being fire-protected and the beam being axially restrained has been modelled adequately in terms of their thermal and structural responses. The consequence of the beam being restrained is that the axial force in the restrained beam starts as a compression, which increases gradually up to a point when the material has deteriorated to such a level that the beam deflects excessively. The axial compression force drops rapidly and changes into a tension force leading to a catenary action, which slows down the beam deflection from running away. Design engineers will be benefited with the consideration of the catenary action.

• Geomechanics and Engineering
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• 제12권2호
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• pp.295-312
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• 2017

Cast in situ and grouted concrete helical piles with 150-200 mm diameter half cylindrical ribs have become an economical and effective choice in Shanghai, China for uplift piles in deep soft soils. Though this type of pile has been successful used in practice, the reinforcing mechanism and the contribution of the ribs to the total resistance is not clear, and there is no clear guideline for the design of such piles. To study the inclusion of ribs to the contribution of shear resistance, the shear behaviour between silty sand and concrete slabs with parallel ribs at different spacing and angles were tested in a large direct shear box ($600mm{\times}400mm{\times}200mm$). The front panels of the shear box are detachable to observe the soil deformation after the test. The tests were modelled with three-dimensional finite element method in ABAQUS. It was found that, passive zones can be developed ahead of the ribs to form undulated failure surfaces. The shear resistance and failure mode are affected by the ratio of rib spacing to rib diameter. Based on the shape and continuity of the failure zones at the interface, the failure modes at the interface can be classified as "punching", "local" or "general" shear failure respectively. With the inclusion of the ribs, the pull out resistance can increase up to 17%. The optimum rib spacing to rib diameter ratio was found to be around 7 based on the observed experimental results and the numerical modelling.